QUANTITATIVE HISTOCHEMISTRY OF RACHITIC RAT CARTILAGE ENZYME ACTIVITY DURING HEALING INDUCED BY VITAMIN D AND STARVATION

Quantitative histochemical methods were used for assaying several dehydrogenases in various cell zones of the rachitic rat epiphyseal cartilage during healing brought about by either vitamin D administration or fasting for 48 hr. 6-Phosphogluconic dehydrogenase activity was significantly greater in the cells of both the proliferating and hypertrophic zones of rachitic control rats as compared to those from vitamin D-treated or fasted rats. Activity of 6-phosphogluconic dehydrogenase in the cells from proliferating and hypertrophic zones was identical in the different groups. Lactic dehydrogenase activity of proliferating and hypertrophic cells obtained from rachitic controls was markedly higher than those from the fasted animals. Minor differences were noted in the malic and isocitric dehydrogenases and peptidase in the cartilage cells obtained from vitamin D-treated, untreated and fasted rats.

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QUANTITATIVE HISTOCHEMISTRY OF THE NEPHRON. III. LACTIC DEHYDROGENASE ACTIVITY IN MAN AND OTHER SPECIES*

Journal of Clinical Investigation ◽

10.1172/jci104157 ◽

1960 ◽

Vol 39 (9) ◽

pp. 1381-1385 ◽

Cited By ~ 26

Author(s):

Sjoerd L. Bonting ◽

Victor E. Pollak ◽

Robert C. Muehrcke ◽

Robert M. Kark

Keyword(s):

Dehydrogenase Activity ◽

Lactic Dehydrogenase ◽

Quantitative Histochemistry

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THE QUANTITATIVE HISTOCHEMISTRY OF HYPOTHALAMUS I. PENTOSE SHUNT ENZYMES IN THE ACTIVATED SUPRAOPTIC NUCLEUS OF THE RAT

Journal of Histochemistry & Cytochemistry ◽

10.1177/15.7.394 ◽

1967 ◽

Vol 15 (7) ◽

pp. 394-398 ◽

Cited By ~ 24

Author(s):

JOHAN F. JONGKIND

Keyword(s):

Dehydrogenase Activity ◽

Supraoptic Nucleus ◽

Lactic Dehydrogenase ◽

Glycolytic Enzyme ◽

Adjacent Area ◽

Hypothalamic Area ◽

Quantitative Histochemistry ◽

Phosphogluconate Dehydrogenase ◽

Nucleus Supraopticus ◽

Glucose 6 Phosphate Dehydrogenase

The activities of two enzymes involved in the oxidative part of the pentose cycle and one glycolytic enzyme have been measured by quantitative histochemical methods both in histologically pure nucleus supraopticus and in an adjacent area of the anterior hypothalamus of rat. In the nucleus supraopticus, glucose 6-phosphate dehydrogenase activity increased 34% and 6-phosphogluconate dehydrogenase activity declined by 9%, while lactic dehydrogenase activity did not change significantly after a thirsting period of 6 days. The nonsupraoptic, adjacent anterior hypothalamic area did not show significant changes in activity of any of the enzymes studied.

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THE INFLUENCE OF HAEMOGLOBIN-S AND G6PD DEFICIENCY ON THE ACTIVITY OF THE 17β-HYDROXYSTEROID DEHYDROGENASE OF INTACT HUMAN ERYTHROCYTES

Acta Endocrinologica ◽

10.1530/acta.0.0750793 ◽

1974 ◽

Vol 75 (4) ◽

pp. 793-800

Author(s):

A. O. Sogbesan ◽

O. A. Dada ◽

B. Kwaku Adadevoh

Keyword(s):

Enzyme Activity ◽

Dehydrogenase Activity ◽

Sex Steroids ◽

G6pd Deficiency ◽

Incubation Medium ◽

Hydroxysteroid Dehydrogenase ◽

G6pd Activity ◽

Reverse Transformation ◽

Oxidative Transformation ◽

Haemoglobin S

ABSTRACT The 17β-hydroxysteroid dehydrogenase activity in intact erythrocytes of Nigerian patients, in particular with regard to haemoglobin genotypes and G6PD* activity was studied. The G6PD activity of the erythrocyte did not affect the oxidative transformation of testosterone to androstenedione and of oestradiol to oestrone. The reduction (reverse transformation) was inhibited in G6PD-deficient erythrocytes but this inhibition was offset by the addition of 0.025 m glucose to the incubation medium. The per cent oxidation transformation of testosterone was higher in Hb-AA than in Hb-SS erythrocytes. It is suggested that the differences may be a result of either lower enzyme activity in the Hb-SS erythrocytes or of differences in the uptake and possibly binding of sex steroids by intact Hb-SS and Hb-AA erythrocytes.

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Serum lactic dehydrogenase activity in hepatic disorders in infancy and childhood

The Indian Journal of Pediatrics ◽

10.1007/bf02835151 ◽

1973 ◽

Vol 40 (9) ◽

pp. 305-311

Author(s):

Ishwar Chandra ◽

S. Inamdar ◽

A. T. Dabke ◽

M. A. Khan ◽

P. S. Mathur

Keyword(s):

Dehydrogenase Activity ◽

Lactic Dehydrogenase ◽

Serum Lactic Dehydrogenase ◽

Infancy And Childhood

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Changes in 3β-hydroxy-Δ5-steroid dehydrogenase activity in granulosa tissue during the ovulatory cycle of the laying hen (Gallus domesticus)

Journal of Endocrinology ◽

10.1677/joe.0.1060269 ◽

1985 ◽

Vol 106 (3) ◽

pp. 269-273 ◽

Cited By ~ 3

Author(s):

D. G. Armstrong

Keyword(s):

Enzyme Activity ◽

Dehydrogenase Activity ◽

Domestic Fowl ◽

Ovarian Follicle ◽

Laying Hen ◽

Gallus Domesticus ◽

Ovulatory Cycle ◽

Lh Surge ◽

Maximal Plasma ◽

Steroid Dehydrogenase

ABSTRACT The object of this study was to examine changes in the activity of granulosa 3β-hydroxy-Δ5-steroid dehydrogenase during the ovulatory cycle of the domestic fowl. The enzyme activity in granulosa tissue from the largest follicle increased significantly during the period 8–14 h before an expected ovulation. The increase in activity occurs before the preovulatory surge of LH and near the time of lights off. During the 4–8 h period before an ovulation, i.e. the time of maximal plasma LH concentrations, 3β-hydroxy-Δ5-steroid dehydrogenase activity decreased in granulosa tissue from the largest follicle. This observation is explained by proposing that the enzyme is inhibited by the large amounts of progesterone found in the tissue at this time. The results indicate that important biochemical changes are taking place within granulosa tissue of the largest ovarian follicle before the preovulatory LH surge. J. Endocr. (1985) 106, 269–273

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