Serum SCC-Ag in Head and Neck Squamous Cell Carcinoma

1990 ◽  
Vol 5 (3) ◽  
pp. 118-120 ◽  
Author(s):  
F. Palermo ◽  
A. Carniato ◽  
A. Fede ◽  
F. Boccaletto ◽  
C. Marchiori
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cancer Patients ◽  
Squamous Cell ◽  
Serum Levels ◽  
Neck Squamous Cell Carcinoma ◽  
Control Group ◽  
Double Antibody ◽  
Combined Evaluation

We estimated the serum levels of SCC-Ag, CEA and TPA in 69 patients with head or neck neoplasia and 31 healthy patients using a radioimmunometric method (double antibody). SCC-Ag concentrations were significantly increased in 43.4% cancer patients with respect to the cut-off point value (1.7 ng/ml) of the control group, and the specificity was 96.7%. The data varied according to the evolutive phase of disease. Since the combined evaluation of SCC-Ag, TPA and CEA serum levels increased the sensitivity, that was 71.0%, we thought it opportune to use all these markers in the tumoral pahtology taken into consideration.

scholarly journals Identification and Confirmation of the miR-30 Family as a Potential Central Player in Tobacco-Related Head and Neck Squamous Cell Carcinoma

2021 ◽  
Vol 11 ◽  
Author(s):  
Tingting Zhang ◽  
Xueqin Zhu ◽  
Qiang Sun ◽  
Xing Qin ◽  
Zhen Zhang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Expression Profiles ◽  
Family Members ◽  
Neck Squamous Cell Carcinoma ◽  
Migration And Invasion ◽  
Control Group

Constituents of tobacco that can cause DNA adduct formation and oxidative stress are implicated in the development of head and neck squamous cell carcinoma (HNSCC). However, there are few studies on the mechanism(s) that underlie tobacco-associated HNSCC. Here, we used a model in which tumors were induced in rats using 4-nitroquinoline 1-oxide (4NQO), which mimicked tobacco-related HNSCC, and analyzed the expression profiles of microRNAs and mRNAs. Our results indicated that 57 miRNAs and 474 mRNA/EST transcripts exhibited differential expression profiles between tumor and normal tongue tissues. In tumor tissue, the expression levels of rno-miR-30 family members (rno-miR-30a, rno-miR-30a-3p, rno-miR-30b-5p, rno-miR-30c, rno-miR-30d, rno-miR-30e and rno-miR-30e-3p) were only 8% to 37% of those in the control group. The GO terms enrichment analysis of the differentially expressed miRNAs indicated that oxidation reduction was the most enriched process. Low expression of miR-30 family members in human HNSCC cell lines and tissues was validated by qPCR. The results revealed that the expression of miR-30b-5p and miR-30e-5p was significantly decreased in the TCGA HNSCC dataset and validation datasets, and this decrease in expression further distinguishes HNSCC associated with tobacco use from other subtypes of HNSCC. CCK8, colony formation, transwell migration and HNSCC xenograft tumor assays indicated that miR-30b-5p or miR-30e-5p inhibited proliferation, migration and invasion in vitro, and miR-30b-5p suppressed tumor growth in vivo. Moreover, we uncovered that KRAS might be the potential target gene of miR-30e-5p or miR-30b-5p. Thus, our data clearly showed that decreased expression of miR-30e-5p or miR-30b-5p may play a crucial role in cancer development, especially that of tobacco-induced HNSCC, and may be a novel candidate biomarker and target for this HNSCC subtype.

Serum levels of interleukins 4 and 10 in head and neck squamous cell carcinoma

2011 ◽  
Vol 126 (2) ◽  
pp. 175-179 ◽  
Author(s):  
Z Mojtahedi ◽  
B Khademi ◽  
A Yehya ◽  
A Talebi ◽  
M J Fattahi ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Tumour Progression ◽  
Serum Levels ◽  
Interleukin 10 ◽  
Neck Squamous Cell Carcinoma ◽  
Interleukin 4 ◽  
Tumour Stage

AbstractObjective:There is currently controversy over the association between serum interleukin-4 and -10 levels and head and neck squamous cell carcinoma in patients of different ethnicity. This study aimed to investigate serum levels of these cytokines in Iranian patients with this pathology, and to analyse correlations with tumour location and tumour stage at diagnosis.Design:Serum cytokines levels were quantified using commercial enzyme-linked immunosorbent assays.Subjects:Study groups comprised 93 untreated patients and 53 healthy donors.Results:Serum interleukin-4 levels were significantly increased in patients compared with controls (p < 0.000), but were not significantly associated with tumour stage. Serum interleukin-10 levels were not raised in patients, nor associated with tumour stage.Conclusion:Serum levels of interleukin-4, but not -10, were increased in Iranian head and neck squamous cell carcinoma patients. These data do not support an association of these cytokines with tumour progression; this is consistent with previous findings.

scholarly journals Evaluation of salivary flow in patients during head and neck radiotherapy

2003 ◽  
Vol 17 (2) ◽  
pp. 156-160 ◽  
Author(s):  
Paulo Rogério Ferreti Bonan ◽  
Fábio Ramoa Pires ◽  
Márcio Ajudarte Lopes ◽  
Osvaldo Di Hipólito Jr
Keyword(s):  
Squamous Cell Carcinoma ◽  
Side Effects ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Salivary Flow ◽  
Neck Squamous Cell Carcinoma ◽  
Control Group ◽  
Head And Neck Radiotherapy

Radiotherapy is frequently employed for the treatment of head and neck squamous cell carcinoma. Among the side effects, xerostomia is one of the most important. With the objective of evaluating the role of radiotherapy in salivary flow, we performed three salivary sample collections: at the beginning of, during, and immediately after radiotherapy. The results showed that the salivary flow values of the first collection were very similar to those of the control group. However, during treatment, there was a significant decrease of the salivary flow (p = 0.0008), which continued low immediately after radiotherapy (p = 0.0009). Our study showed that radiotherapy leads to an important reduction of salivary flow during and after radiotherapy.

Serum levels of interleukin-7 and interleukin-8 in head and neck squamous cell carcinoma

2014 ◽  
Vol 51 (3) ◽  
pp. 227 ◽  
Author(s):  
A Ghaderi ◽  
Z Mojtahedi ◽  
Y Taregh ◽  
Z Rafati ◽  
B Khademi ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Serum Levels ◽  
Interleukin 8 ◽  
Neck Squamous Cell Carcinoma ◽  
Interleukin 7

scholarly journals Lipid Peroxidation and Antioxidant Status in Head and Neck Squamous Cell Carcinoma Patients

2009 ◽  
Vol 2 (2) ◽  
pp. 68-72 ◽  
Author(s):  
Aashita Gupta ◽  
Madan L. B. Bhatt ◽  
Mithilesh K. Misra
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Squamous Cell Carcinoma ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cancer Patients ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma

Oxidative stress, a consequence of an imbalance between the formation and inactivation of reactive oxygen species, may be involved in the pathogenesis of many diseases including cancer. To evaluate the magnitude of oxidative stress, a study on the plasma levels of superoxide dismutase, total thiols, ascorbic acid and malondialdehyde (MDA) has been done in head and neck squamous cell carcinoma patients before the start of any oncological treatment and compared with healthy controls. The specific activity of superoxide dismutase in cancer patients is decreased significantly when compared to the control (p < 0.05). The total thiol and ascorbic acid levels are significantly reduced (p < 0.005) whereas MDA levels are significantly increased in the patients (p < 0.00005). Our findings show that the oxidative stress is elevated in cancer patients as evidenced by elevated levels of lipid peroxidation products and depletion of enzymatic and non-enzymatic antioxidants.

scholarly journals A Naturally Occurring Feline Model of Head and Neck Squamous Cell Carcinoma

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Jackie M. Wypij
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cancer Patients ◽  
Squamous Cell ◽  
Human Cancer ◽  
Tumor Model ◽  
Neck Squamous Cell Carcinoma ◽  
Animal Tumor ◽  
Feline Model

Despite advances in understanding cancer at the molecular level, timely and effective translation to clinical application of novel therapeutics in human cancer patients is lacking. Cancer drug failure is often a result of toxicity or inefficacy not predicted by preclinical models, emphasizing the need for alternative animal tumor models with improved biologic relevancy. Companion animals (dogs and cats) provide an opportunity to capitalize on an underutilized and biologically relevant translational research model which allows spontaneous disease modeling of human cancer. Head and neck squamous cell carcinoma (HNSCC) is a common cancer with a poor prognosis and limited clinical advancements in recent years. One potential novel spontaneous animal tumor model is feline oral squamous cell carcinoma (FOSCC). FOSCC and HNSCC share similar etiopathogenesis (tobacco and papillomavirus exposure) and molecular markers (EGFR, VEGF, and p53). Both human and feline SCCs share similar tumor biology, clinical outcome, treatment, and prognosis. Future clinical trials utilizing FOSCC as a tumor model may facilitate translation of preclinical cancer research for human cancer patients.

Plasma Platelet-Derived Growth Factor: Preliminary Study of a Potential Marker in Head and Neck Cancer

1996 ◽  
Vol 105 (9) ◽  
pp. 710-712 ◽  
Author(s):  
Lyon L. Gleich ◽  
Laxmi Srivastava ◽  
Jack L. Gluckman
Keyword(s):  
Squamous Cell Carcinoma ◽  
Growth Factor ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cancer Patients ◽  
Squamous Cell ◽  
Platelet Derived Growth Factor ◽  
Control Group ◽  
The Mean ◽  
Preliminary Study

The role of growth factors in the development and spread of head and neck cancers has received little attention. Platelet-derived growth factor (PDGF) is a potent mitogen that is released normally in wound healing, but is also secreted by human malignant epithelial cells. In breast and ovarian carcinomas, elevated plasma PDGF has correlated with a poorer prognosis. This preliminary study was designed 1) to determine if PDGF is elevated in the plasma of patients with squamous cell carcinoma of the head and neck and 2) to determine whether there is any change in levels following surgical ablation. The PDGF was measured by radioimmunoassay in 18 patients with head and neck squamous cell carcinoma and in 12 normal controls. In the control group the mean level was 7.4 fmol/100 μL (range 2.8 to 12.5, median 7.6), in spite of the fact that PDGF is reportedly not measurable in normal subjects. The mean PDGF level in the cancer patients was 20.4 fmol/100 μL (range 4.1 to 35.7, median 20.5) and as compared to the control group was significantly elevated (median two-sample test, p < .001). Of the 20 cancer patients, only 4 had levels less than 12.5 fmol/100 μL. Moreover, 2 of these had undergone prior radiotherapy. In all cases, PDGF levels decreased significantly after surgery. These results support the importance of the further investigation of plasma PDGF levels as a potential biomarker to evaluate efficacy of treatment, possibly to aid in the detection of tumor recurrence, and even to be a potential indicator of tumor aggressiveness.

scholarly journals Does Trans-Stimulation of L-Tyrosine Lead to an Increase in Boron Uptake in Head and Neck Squamous Cell Carcinoma Cells?

2021 ◽  
Vol 11 (16) ◽  
pp. 7286
Author(s):  
Matthias Gielisch ◽  
Maximilian Moergel ◽  
Bilal Al-Nawas ◽  
Peer W. Kämmerer
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Inductively Coupled Plasma ◽  
Therapeutic Option ◽  
Neck Squamous Cell Carcinoma ◽  
Control Group ◽  
Boron Uptake

(1) Background: For advanced head and neck squamous cell carcinoma (HNSCC), boron neutron capture therapy (BNCT) is a potential therapeutic option, but high concentrations of boron within HNSCC are necessary. Therefore, this in vitro pilot study examined the uptake and concentration of boron in HNSCC cells using the trans-stimulation effect of L-tyrosine when compared to non-stimulated samples. (2) Methods: Two HNSCC cell lines were incubated with L-tyrosine for up to two hours, followed by incubation with three L-para-boronophenylalanine (BPA) concentrations (5, 20, 50 ppm) at eight incubation times (1–4.5 h in half-hour steps). Subsequently, cellular boron uptake was measured via inductively coupled plasma mass spectrometry. (3) Results: No differences between laryngeal and oral SCC cells were seen; therefore, data were pooled. In total, boron uptake was not significantly increased in trans-stimulated samples when compared to the control group (all p > 0.05). Nevertheless, with trans-stimulation, higher BPA concentrations resulted in higher intracellular boron concentrations (5 < 20 < 50 ppm; all: p < 0.05), whereas these differences were less distinct in the non-trans-stimulated group. (4) Conclusions: The effect of trans-stimulation for up to two hours seems to be less relevant for HNSCC, though trans-stimulated HNSCC cells seem to have a more distinct BPA-dose-dependent cellular boron uptake that might be addressed in further research.

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