TLR4 and TLR9 polymorphism: Probable role in susceptibility among the population of Bihar for Indian visceral leishmaniasis

Genetic variations in the host TLRs genes play an important role in susceptibility and/or resistance to visceral leishmaniasis by altering the host-pathogen interaction. In this study, we investigated the association between polymorphisms of TLR4 (Asp299Gly, Thr399Ile) and TLR-9 (T-1237C), with susceptibility to visceral leishmaniasis. A bi-directional PCR amplification of specific alleles technique was used to characterize the distribution of TLR4 (Asp299Gly and Thr399Ile) and TLR9 (T-1237C) polymorphisms. A total of 60 samples were randomly selected from confirmed visceral leishmaniasis patients and 24 endemic healthy volunteers. The samples were genotyped and allele frequencies were determined. We observed that TLR4 Asp299Gly and Thr399Ile genotypes were more frequent in visceral leishmaniasis patients (10% and 15% respectively) compared to controls (4.2% and 8.3% respectively). However, the differences were not significant in TLR4 Asp299Gly and Thr399Ile alleles and genotypes. In the case of TLR9, we observed the frequency of T1237C genotype was higher in visceral leishmaniasis patients (43.3%) than in healthy controls (33.3%). Statistically significant differences were observed in TLR9 T1237C alleles and genotypes. We concluded that TLR9 T1237C, but not TLR4, gene polymorphisms can be regarded as contributors to visceral leishmaniasis susceptibility among the Indian population of Bihar state.

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The TLR2 and TLR4 gene polymorphisms in Moroccan visceral leishmaniasis patients

Acta Tropica ◽

10.1016/j.actatropica.2016.02.020 ◽

2016 ◽

Vol 158 ◽

pp. 77-82 ◽

Cited By ~ 4

Author(s):

Rajaâ Ejghal ◽

Moustapha Hida ◽

Mounya Lahkim Bennani ◽

Mariame Meziane ◽

Rabia Aurag ◽

...

Keyword(s):

Visceral Leishmaniasis ◽

Gene Polymorphisms ◽

Tlr4 Gene

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CD14 and TLR4 Gene Polymorphisms in Adult Periodontitis

Journal of Dental Research ◽

10.1177/154405910508401114 ◽

2005 ◽

Vol 84 (11) ◽

pp. 1042-1046 ◽

Cited By ~ 52

Author(s):

M.L. Laine ◽

S.A. Morré ◽

L.S. Murillo ◽

A.-J. van Winkelhoff ◽

A.S. Peña

Keyword(s):

Gene Polymorphisms ◽

Genetic Factors ◽

Healthy Controls ◽

Toll Like Receptor ◽

Periodontal Pathogens ◽

Toll Like Receptor 4 ◽

Severe Periodontitis ◽

Tlr4 Gene ◽

Host Genetic ◽

Host Genetic Factors

Bacterial deposits, smoking, and host genetic factors play a major role in an individual’s predisposition to periodontitis. Bacterial components are recognized by CD14 and toll-like receptor 4 (TLR4), resulting in a NF-κB-based inflammatory response. We hypothesized that functional CD14 and TLR4 polymorphisms contribute to periodontitis susceptibility. We aimed to investigate the occurrence of CD14-260C>T, TLR4 299Asp>Gly, and 399Thr>Ile gene polymorphisms in adult periodontititis. DNA was collected from 100 patients with severe periodontitis and from 99 periodontally healthy controls. The gene polymorphisms were determined by the PCR technique. The presence of the periodontal pathogens Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, and whether the subjects smoked, was included in the analyses. The CD14-260T/T genotype was found in 34.0% of periodontitis patients and in 20.2% of controls. Logistic regression analysis adjusted for gender, age, smoking, and prevalence of P. gingivalis and A. actinomycetemcomitans showed an association between the CD14-260T/T genotype and periodontitis (P = 0.004, OR 3.0, 95% CI 1.4–6.9). We conclude that the CD14-260T/T genotype contributes to the susceptibility to severe periodontitis in Dutch Caucasians.

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Modulation of Plasma Fibrinogen Levels by Ticlopidine in Healthy Volunteers and Patients with Stable Angina Pectoris

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650547 ◽

1996 ◽

Vol 76 (02) ◽

pp. 166-170 ◽

Cited By ~ 11

Author(s):

Moniek P M de Maat ◽

Alf E R Arnold ◽

Stef van Buuren ◽

J H Paul Wilson ◽

Cornells Kluft

Keyword(s):

Angina Pectoris ◽

Healthy Volunteers ◽

Acute Phase ◽

Stable Angina ◽

Gene Polymorphisms ◽

Acute Phase Reaction ◽

Plasma Fibrinogen ◽

Phase Reaction ◽

Stable Angina Pectoris ◽

Lowering Effect

SummaryElevated plasma fibrinogen levels are associated with an increased risk for cardiac events. Ticlopidine is a drug that inhibits the ADP-induced aggregation of blood platelets and it also has been described that ticlopidine can decrease the plasma fibrinogen level in patients with vascular diseases. The mechanism of this decrease has not yet been elucidated and therefore mechanisms that are known to affect fibrinogen levels were studied, viz. the acute phase reaction, total fibrin plus fibrinogen degradation (TDP) levels and the polymorphisms of the fibrinogen β-gene.The fibrinogen lowering effect of ticlopidine was studied in 26 healthy volunteers, selected on genotype of the BclI polymorphism of the fibrinogen β-gene, and in 26 patients with stable angina pectoris in a double blind, randomized cross-over study. Functional plasma fibrinogen levels were measured with the Clauss assay. Fibrinogen antigen, C-reactive protein (CRP) and TDP levels were measured using an enzyme immuno assay (EIA).In the healthy volunteers the functional fibrinogen levels had decreased by 0.20 g/l (9%, p = 0.005 using the paired Student t-test) after 4 weeks of 250 mg bid ticlopidine administration, whereas fibrinogen antigen, CRP and TDP levels were not significantly changed. In the stable angina pectoris patients the pre-treatment fibrinogen, CRP and TDP levels were significantly higher than in the volunteer group. After four weeks 250 mg bid ticlopidine administration the functional fibrinogen levels had decreased by 0.38 g/l (11%, p < 0.005), whereas the fibrinogen antigen, CRP and TDP levels were not significantly changed. The levels of functional and antigen fibrinogen, CRP and TDP did not change significantly during the placebo period in the volunteers or the patients. Neither in the volunteers nor in the patients was the effect of ticlopidine on the fibrinogen levels associated with the fibrinogen β-gene polymorphisms.Therefore, the fibrinogen lowering effect of ticlopidine is likely to be a modulation of the functionality of the molecule and unlikely to be modulated by the acute phase reaction, TDP-levels or the fibrinogen β-gene polymorphisms.

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Gene polymorphisms associated with an increased risk of exudative age-related macular degeneration in a Spanish population

European Journal of Ophthalmology ◽

10.1177/11206721211002698 ◽

2021 ◽

pp. 112067212110026

Author(s):

Pablo Gili ◽

Leyre Lloreda Martín ◽

José-Carlos Martín-Rodrigo ◽

Naon Kim-Yeon ◽

Laura Modamio-Gardeta ◽

...

Keyword(s):

Macular Degeneration ◽

Gene Polymorphisms ◽

Age Related Macular Degeneration ◽

Spanish Population ◽

Healthy Controls ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Exudative Amd ◽

Age Related ◽

Increased Risk

Purpose: To identify the association between single-nucleotide polymorphisms (SNPs) in CFH, ARMS2, HTRA1, CFB, C2, and C3 genes and exudative age-related macular degeneration (AMD) in a Spanish population. Methods: In 187 exudative AMD patients and 196 healthy controls (61% women, mean age 75 years), 12 SNPs as risk factors for AMD in CFH (rs1410996, rs1061170, r380390), ARMS2 (rs10490924, rs10490923), HTRA1 (rs11200638), CFB (rs641153), C2 (rs547154, rs9332739), and C3 (rs147859257, rs2230199, rs1047286) genes were analyzed. Results: The G allele was the most frequent in CFH gene (rs1410996) with a 7-fold increased risk of AMD (OR 7.69, 95% CI 3.17–18.69), whereas carriers of C allele in CFH (rs1061170) showed a 3-fold increased risk for AMD (OR 3.22, 95% CI 1.93–5.40). In CFH (rs380390), the presence of G allele increased the risk for AMD by 2-fold (OR 2.52, 95% CI 1.47–4.30). In ARMS2 (rs10490924), the T-allele was associated with an almost 5-fold increased risk (OR 5.49, 95% CI 3.23–9.31). The A allele in HTRA1 (rs11200638) was more prevalent in AMD versus controls (OR 6.44, 95% CI 3.62–11.47). In C2 gene (rs9332739) the presence of C increased risk for AMD by 3-fold (OR 3.10, 95% CI 1.06–9.06). Conclusion: SNPs in CFH, ARMS2, HTRA1, and C2 genes were associated in our study with an increased risk for exudative AMD in Spanish patients.

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Association of TLR4 gene polymorphisms with sepsis after a burn injury: findings of the functional role of rs2737190 SNP

Genes and Immunity ◽

10.1038/s41435-021-00121-z ◽

2021 ◽

Author(s):

Claudia A. Colín-Castro ◽

Rafael Franco-Cendejas ◽

Hector I. Rocha-González ◽

Esteban Cruz-Arenas ◽

Norberto Leyva-García ◽

...

Keyword(s):

Gene Polymorphisms ◽

Functional Role ◽

Burn Injury ◽

Tlr4 Gene

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Association between apolipoprotein gene polymorphisms and hyperlipidemia: a meta-analysis

BMC Genomic Data ◽

10.1186/s12863-021-00968-1 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Xiao-Ning Zhao ◽

Quan Sun ◽

You-Qin Cao ◽

Xiao Ran ◽

Yu Cao

Keyword(s):

Risk Factor ◽

Cerebrovascular Disease ◽

Gene Polymorphisms ◽

Meta Analysis ◽

Control Group ◽

Case Group ◽

Healthy Controls ◽

Ε4 Allele ◽

Different Populations ◽

The Impact

Abstract Background Hyperlipidemia plays an important role in the etiology of cardio-cerebrovascular disease. Over recent years, a number of studies have explored the impact of apolipoprotein genetic polymorphisms in hyperlipidemia, but considerable differences and uncertainty have been found in their association with different populations from different regions. Results A total of 59 articles were included, containing in total 13,843 hyperlipidemia patients in the case group and 15,398 healthy controls in the control group. Meta-analysis of the data indicated that APOA5–1131 T > C, APOA1 -75 bp, APOB XbaI, and APOE gene polymorphisms were significantly associated with hyperlipidemia, with OR values of 1.996, 1.228, 1.444, and 1.710, respectively. All P-values were less than 0.05. Conclusions Meta-analysis of the data indicated that the C allele of APOA5 1131 T > C, the A allele at APOA1-75 bp, the APOB XbaI T allele, and the ε2 and ε4 allele of APOE were each a risk factor for susceptibility for hyperlipidemia.

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Association between TLR2 and TLR4 Gene Polymorphisms and the Susceptibility to Inflammatory Bowel Disease: A Meta-Analysis

PLoS ONE ◽

10.1371/journal.pone.0126803 ◽

2015 ◽

Vol 10 (5) ◽

pp. e0126803 ◽

Cited By ~ 22

Author(s):

Yang Cheng ◽

Yun Zhu ◽

Xiuping Huang ◽

Wei Zhang ◽

Zelong Han ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Bowel Disease ◽

Gene Polymorphisms ◽

Meta Analysis ◽

Tlr4 Gene ◽

Inflammatory Bowel

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Tumor necrosis factor-α and interleukin-1β gene polymorphisms and risk of brain abscess in North Indian population

Cytokine ◽

10.1016/j.cyto.2015.07.009 ◽

2015 ◽

Vol 75 (1) ◽

pp. 159-164 ◽

Cited By ~ 4

Author(s):

Priyanka Mishra ◽

Kashi Nath Prasad ◽

Kamini Singh ◽

Anamika Bajpai ◽

Rabi Narayan Sahu ◽

...

Keyword(s):

Tumor Necrosis Factor ◽

Brain Abscess ◽

Gene Polymorphisms ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Indian Population ◽

Interleukin 1Β ◽

North Indian Population ◽

Factor Α ◽

Necrosis Factor

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Lingual–Alveolar Contact Pressure During Speech in Amyotrophic Lateral Sclerosis: Preliminary Findings

Journal of Speech Language and Hearing Research ◽

10.1044/2016_jslhr-s-16-0107 ◽

2017 ◽

Vol 60 (4) ◽

pp. 810-825 ◽

Cited By ~ 5

Author(s):

Jeff Searl ◽

Stephanie Knollhoff ◽

Richard J. Barohn

Keyword(s):

Amyotrophic Lateral Sclerosis ◽

Healthy Volunteers ◽

Contact Pressure ◽

Statistical Difference ◽

Healthy Controls ◽

Perceptual Judgments ◽

Before And After ◽

Preliminary Study ◽

Contact Pressures ◽

Lateral Sclerosis

Purpose This preliminary study on lingual–alveolar contact pressures (LACP) in people with amyotrophic lateral sclerosis (ALS) had several aims: (a) to evaluate whether the protocol induced fatigue, (b) to compare LACP during speech (LACP-Sp) and during maximum isometric pressing (LACP-Max) in people with ALS (PALS) versus healthy controls, (c) to compare the percentage of LACP-Max utilized during speech (%Max) for PALS versus controls, and (d) to evaluate relationships between LACP-Sp and LACP-Max with word intelligibility. Method Thirteen PALS and 12 healthy volunteers produced /t, d, s, z, l, n/ sounds while LACP-Sp was recorded. LACP-Max was obtained before and after the speech protocol. Word intelligibility was obtained from auditory–perceptual judgments. Results LACP-Max values measured before and after completion of the speech protocol did not differ. LACP-Sp and LACP-Max were statistically lower in the ALS bulbar group compared with controls and PALS with only spinal symptoms. There was no statistical difference between groups for %Max. LACP-Sp and LACP-Max were correlated with word intelligibility. Conclusions It was feasible to obtain LACP-Sp measures without inducing fatigue. Reductions in LACP-Sp and LACP-Max for bulbar speakers might reflect tongue weakness. Although confirmation of results is needed, the data indicate that individuals with high word intelligibility maintained LACP-Sp at or above 2 kPa and LACP-Max at or above 50 kPa.

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