scholarly journals Occurrence of Sesquiterpene Derivatives in Scleria Striatonux De Wild (Cyperaceae)

2009 ◽  
Vol 4 (1) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Kennedy Nyongbela ◽  
Karine Ioset Ndjoko ◽  
Reto Brun ◽  
Sergio Wittlin ◽  
James Mbah ◽  
...  
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Structure Elucidation ◽  
Methylene Chloride ◽  
Methanol Extract ◽  
Antiplasmodial Activity ◽  
Spectral Studies ◽  
Preparative Hplc ◽  
Combination Of Methods

Scleria striatonux (Cyperaceae), a spice traditionally used in Cameroon, displayed an antiplasmodial activity. Two new sesquiterpenes, 1 (sclerienone A) and 2 (sclerienone B), have subsequently been isolated from the methylene chloride/methanol extract of the rhizome. Their separation involved a combination of methods including repeated silica gel open column chromatography, Sephadex LH-20 and semi-preparative HPLC. Structure elucidation was achieved by means of chemical tests and extensive spectral studies.

scholarly journals Isolation and Characterization of Sclerienone C from Scleria Striatinux

2016 ◽  
Vol 11 (1) ◽  
pp. 1934578X1601100
Author(s):  
Kennedy D. Nyongbela ◽  
Felix L. Makolo ◽  
Thomas R. Hoye ◽  
Simon MN Efange
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Structure Elucidation ◽  
Spectroscopic Analysis ◽  
Methylene Chloride ◽  
Isolation And Purification ◽  
Isolation And Characterization ◽  
Combination Of Methods

Herein, we report the isolation and characterization of sclerienone C, a novel sesquiterpene isolated from the methylene chloride/methanol (1:1) extract of Scleria striatinux that we have deduced to have structure 1. This medicinal spice of Cameroon has been shown to display antimicrobial and antiplasmodial activities. The isolation and purification involved a combination of methods including silica gel column chromatography, Sephadex LH-20, and semi-prep HPLC separations. Structure elucidation was carried-out by means of spectroscopic analysis and comparison with previously isolated sesquiterpene derivatives from the plant.

A Cytotoxic Natural Product from Patrinia villosa Juss

2019 ◽  
Vol 19 (11) ◽  
pp. 1399-1404 ◽  
Author(s):  
Yangcheng Liu ◽  
Wei Liu ◽  
Changlan Chen ◽  
Zheng Xiang ◽  
Hongwei Liu
Keyword(s):  
Cell Cycle ◽  
Cell Lines ◽  
Silica Gel ◽  
Column Chromatography ◽  
Bioactive Compound ◽  
Antitumor Agent ◽  
2D Nmr ◽  
Preparative Hplc ◽  
Dependent Manner ◽  
2D Nmr Spectra

Background and Purpose:: Patrinia villosa Juss is an important Chinese herbal medicine widely used for thousands of years, but few reports on the ingredients of the herb have been presented. In this study, we aim to isolate the bioactive compound from the plant. Material and Methods:: The air-dried leaves of P. villosa (15kg) were extracted three times with 70% EtOH under reflux. The condensed extract was suspended in H2O and partitioned with light petroleum, dichloromethane and n-BuOH. The dichloromethane portion was then subjected to normal-phase silica gel column chromatography, ODS silica gel column chromatography and semi-preparative HPLC to yield compound 1. Cytotoxicities of 1 were assayed on HepG2, A549 and A2780 cell lines. The mechanism of apoptosis and cell cycle on A549 was confirmed subsequently. Results: A new impecylone (Impecylone A) was isolated from the leaves of Patrinia villosa Juss, and its structures were established using 1D, 2D-NMR spectra and HR-ESI-MS. Impecylone A could selectivity inhibit HepG2 and A549 cell lines. The compound could induce apoptosis of A549 and arrest the cell cycle at G2/M phase in a dose-dependent manner. Conclusion: Impecylone A is a novel compound from Patrinia villosa Juss and could be a potential antitumor agent especially in the cell lines of A549.

Isolation of the antidiarrhoeal tiliroside and its derivative from Waltheria indica leaf extract

2021 ◽  
Vol 25 (1) ◽  
pp. 86-92
Author(s):  
B.A. Ayinde ◽  
J.O. Owolabi ◽  
I.S. Uti ◽  
P.C. Ogbeta ◽  
M.I. Choudhary
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Preparative Thin Layer Chromatography ◽  
Inhibitory Effects ◽  
Aqueous Fraction ◽  
Vacuum Liquid Chromatography ◽  
Ileum Contraction ◽  
Layer Chromatography ◽  
Dose Dependent

The antidiarrhoeal effect of Waltheria indica methanol extract and fractions have been reported earlier but, the present work examined the intestinal relaxant effects of two flavonoid-phenyl propanoids isolated from the methanol extract. The active aqueous fraction was subjected to vacuum liquid chromatography using dichloromethane with increasing concentration of ethyl acetate, and that of methanol and water successively. The ten (10) fractions obtained were combined to give seven (7). The fraction 2 (C, D) was subjected to preparative thin layer chromatography on silica gel GF254 (10-40μm) using CHCl3-CH3OH (8:2) to obtain compound coded F2. Fraction 4 (F) was subjected to column chromatography using silica gel (60-120μm mesh) and eluted with  dichloromethane with increasing concentrations of methanol. Fractions 9-28 were combined and subjected to column  chromatography using chloroform with increasing concentration of methanol. The fractions 1-16 of these were purified on Sephadex LH-20 to obtain compound BAA. The identities of the two compounds were established using spectroscopic methods. The  antidiarrheal effect of compound F2 was evaluated on mice using charcoal transit (100,200, 400mg/kg), castor oil (40, 60 mg/kg)  while the two compounds were examined for their inhibitory effects on Ach-induced ileum contraction. The effects of the  compounds were compared with loperamide (3mg/kg) and atropine (80μg). Compounds F2 and BAA were identified as tiliroside and 3’’’, 5’’’-dimethoxy tiliroside respectively. Tiliroside inhibited the charcoal transition in the animals in a dose dependent pattern with 400mg/ mL eliciting 63.41% inhibition compared to 59.23% produced by loperamide. The compound also elicited significantly (P<0.05) prolonged onset of stooling and reduced the number and weight of stools produced lower than the control. The two  compounds drastically inhibited the Ach-induced contractions of the ileum. The compound, tiliroside at 10mg, completely abolished  the contraction by Ach unlike 3’’’, 5’’’-dimethoxy tiliroside which reduced the contraction to 1.92% at 20mg. The identified compounds seem to be responsible for the ethnomedicinal use of the plant in treating diarrhea.

scholarly journals Isolation and Structure Elucidation of Soulatro Coumarin From Stem Bark of Calophyllum soulattri Burm F and In Vivo Antiplasmodial Activity by Using Mice Infected by Plasmodium berghei

2011 ◽  
Vol 2 (2) ◽  
pp. 242
Author(s):  
Jamilah Abbas ◽  
Achmad Darmawan ◽  
Syafruddin Syafruddin
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Column Chromatography ◽  
Plasmodium Berghei ◽  
2D Nmr ◽  
Stem Bark ◽  
Antiplasmodial Activity ◽  
Nmr Techniques ◽  
Coumarin Compound

The soulatro coumarin compound was isolated and elucidated from the stem bark of Calophyllum soulattri Burm F, the samples were collected from Jayapura Papua Irian Island in Indonesia. Isolation process was done by maceration at room temperature in methanol, than partitioned in a mixture of n hexane-water (1:1), followed by dichloromethane-water (1:1)  and ethyl acetate-water (1:1). A portion of ethyl acetate extract was subjected to column chromatography over silica gel packed and eluted with n-hexane a gradient of ethyl acetate to 100% followed by CHCl3  in MeOH (20:1, 10 :1, 5:1, 1:1). Fraction  B (CHCl3 in MeOH 20:1) was subjected to column chromatography  over silica gel 300 mesh  and eluted with EtOAc-MeOH mixtures of increasing polarity. Faction with the same Rf valeus were combined and eluted with EtOAc-MeOH  (19:1) showed one spot on TLC. They were combined and evaporated to yield a solid than was recrystallized in mixture of CH2Cl2-methanol to give soulatro coumarin compound. The structure was determinated by spectroscopic analysis, in particular by 1D and 2D NMR techniques, from these spectra data conclution that compound is soulatro coumarin. Antimalarial assay was tested against Plasmodium berghei parasite as in vivo using 18 mices rodent wich was infected by  Plasmodium berghei parasite. The soulatro coumarin  showed activity against P. berghei with dosage 0.0005867 mM/1 kg body weight ; 0.005867 mM/1 kg bw; 0.05867 mM/1 kg bw; 0.5867 mM/1 kg bw 5.867 mM/1 kg bw and 58.67 mM/1 kg bw could inhibite growth rate of parasite = 57.32%; 63.37%; 43.02%; 53.49%; 47.67% respectively.Keywords : Antiplasmodial activity, coumarin, Calophyllum soulattri Burm. F, in vivo, Chloroquine, Plasmodium berghei.

scholarly journals New dibenzocyclooctadiene lignan from Schisandra chinensis (Turcz.) Baill. fruits

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Trong Nguyen Nguyen ◽  
Yeong-Geun Lee ◽  
Hyoung-Geun Kim ◽  
Dahye Yoon ◽  
Jin Tae Jeong ◽  
...  
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Absolute Configuration ◽  
Preparative Hplc ◽  
Schisandra Chinensis ◽  
Axial Chirality ◽  
Chemical Structures ◽  
Data Analyses ◽  
Nmr Data ◽  
Hplc Column

AbstractRepeated column chromatography using Sephadex LH-20, silica gel (SiO2), and octadecyl SiO2 (ODS) as well as preparative HPLC column chromatography led to isolation of a new dibenzocyclooctadiene lignan along with four known ones, gomisin L2 (1), L1 (2), M1 (3), and M2 (4). Their chemical structures were fixed based on MS, IR, and NMR data analyses. In addition, the stereochemistry of atropisomers, the absolute configuration of the axial chirality in a biphenyl structure, was confirmed by a CD experiment. The new lignan was named gomisin M3 (5).

scholarly journals IN VITRO ANTIPLASMODIAL ACTIVITY OF FLOWER EXTRACTS FROM COMBRETUM LEPROSUM MART (MOFUMBO)

2016 ◽  
Vol 39 (1) ◽  
pp. 84 ◽  
Author(s):  
Guilherme Matos Passarini ◽  
Daniel Sol Medeiros ◽  
Dionatas Ulisses de Oliveira Meneguetti ◽  
Renato Abreu Lima ◽  
Valdir Alves Facundo ◽  
...  
Keyword(s):  
At Risk ◽  
Silica Gel ◽  
Column Chromatography ◽  
Chloroform Extract ◽  
Antiplasmodial Activity ◽  
Pharmacological Studies ◽  
Combretum Leprosum ◽  
Resistant Strains ◽  
Active Substances

Malaria is the cause of hundreds of deaths per year , besides putting billions of people at risk of developing disease. When it comes to its therapy, the drugs used currently are losing its efficacy due to increase inn the frequency of resistant strains of the parasite, highlight the importance for the serach of new classes of molecules prsentign antiplasmodial activity. In the present work, the antiplasmodial activities of five extracts from the flowers of Comretum leprosum are described. The method employed for obtaingine the extracts was silica gel column chromatography, and the techniques used for the analysis of antiplasmodial activity and citotoxicity were ELISA and MTT respectively, were a selectivitu index was calculated after the obtainign of these two values. The extract presenting the highest antiplasmodial activity was the chloroform extract, however, this extrac also presented the higther cytotoxicity and therefore the extract presenting the best overall activity was the hexane extract. The study deminstrated the plant Combretum leprosum has active substances against P. falciparum and therefore is a potential to be expored in funther pharmacological studies.

Cycloartane triterpenoid saponins from the roots of Astragalus ernestii

2020 ◽  
Vol 75 (9-10) ◽  
pp. 821-824
Author(s):  
Jin-Qiang Li ◽  
Chao-Jiang Xiao ◽  
Han Yang ◽  
Xin-Yan Tian ◽  
Ye-Meng Li ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Silica Gel ◽  
Column Chromatography ◽  
Systematic Study ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Triterpenoid Saponins ◽  
Cycloasgenin C

AbstractA systematic study on chemical constituents of the roots of Astragalus ernestii was performed by silica gel, sephadex LH-20, MCI gel and Rp-18 column chromatography and spectral analysis such as MS, 1D and 2D NMR. As a result, nine cycloartane triterpenoid saponins were obtained from the methanol extract including a new compound asernestioside D (1), along with eight known constituents, 3-O-β-d-xylopyranosyl-3β,6a,16β,20(S),24(R),25-hexahydroxycycloartane (2), cycloasgenin C-3-O-β-d-xylopyranoside (3), cycloascidoside (4), cyclosiversigenin-3-O-β-d-xylopyranoside (5), isoastragaloside IV (6), asernestioside A (7), asernestioside B (8), and asernestioside C (9).

scholarly journals Determination of Polysorbate 60 in Salad Dressings by Colorimetric and Thin Layer Chromatographic Techniques

1982 ◽  
Vol 65 (1) ◽  
pp. 162-165
Author(s):  
Daniel H Daniels ◽  
Charles R Warner ◽  
Sami Selim
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
Column Chromatography ◽  
Chromatographic Analysis ◽  
Methylene Chloride ◽  
Chromatographic Techniques ◽  
Methylene Chloride Extract ◽  
Dragendorff Reagent ◽  
Thin Layer Chromatographic Analysis

Abstract Methods are described for the quantitation and verification of polysorbate 60 (PS 60) in salad dressing. The sample is partitioned between methylene chloride and water. The methylene chloride extract is further treated by silica gel column chromatography. The isolated PS 60 is complexed with ammonium cobaltothiocyanate and determined spectrophotometrically at 620 nm. Additional evidence indicating the presence of PS 60 is obtained from thin layer chromatographic analysis using the modified Dragendorff reagent for visualization of spots.

Grandidentatin isomer from bark of suwon poplar (Populus alba L.×Populus glandulosa Uyeki)

Holzforschung ◽  
2009 ◽  
Vol 63 (3) ◽  
Author(s):  
Jin-Kyu Kim ◽  
Young-Soo Bae
Keyword(s):  
Nmr Spectroscopy ◽  
Silica Gel ◽  
Column Chromatography ◽  
Structure Elucidation ◽  
Hybrid Poplar ◽  
2D Nmr ◽  
Populus Alba ◽  
Phenolic Glucoside

Abstract The phenolic glucoside cis-2-hydroxycyclohexyl 6-O-p-coumaroyl-β-d-glucopyranoside was isolated from the bark of the hybrid poplar Populus alba×glandulosa by column chromatography over Sephadex LH-20 and Silica gel preparative TLC. The structure elucidation was performed by NMR spectroscopy (2D NMR COSY, DEPT, HMQC, and HMBC) and MS spectrometry.

scholarly journals Studies on the chemical compositions of Hyptis suaveolens (L.) Poit

2019 ◽  
Vol 84 (3) ◽  
pp. 245-252 ◽  
Author(s):  
Gengqiu Tang ◽  
Xile Liu ◽  
Xue Gong ◽  
Xiaojing Lin ◽  
Xiudi Lai ◽  
...  
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Scientific Basis ◽  
Chemical Compositions ◽  
Preparative Hplc ◽  
Chemical Structures ◽  
Aerial Parts ◽  
Medicinal Value ◽  
Hyptis Suaveolens ◽  
First Time

This study aimed at identifying the chemical compounds isolated from the aerial parts and roots of Hyptis suaveolens (L.) Poit. The compounds were isolated and purified by a combination of various chromatographic techniques including silica gel column chromatography, silica gel reverse phase column chromatography, Sephadex LH-20 gel column chromatography, semi-preparative HPLC and recrystallization. The chemical structures were analyzed and elucidated based on physiochemical properties and spectroscopic analysis. From the aerial parts of H. suaveolens, eight compounds were isolated and identified as quercetin 3-O-?-D-glucopyranoside (1), apigenin (2), sorbifolin (3), quercetin (4), kaempferol (5), genkwanin (6), rosmarinic acid (7) and methyl rosmarinate (8). Two compounds were isolated from the roots of H. suaveolens and identified as podophyllotoxin (9) and picropodophyllotoxin (10). Compounds 2?6 were isolated from H. suaveolens for the first time while compound 10 was isolated from the genus of Hyptis for the first time. The results of this study provided a scientific basis for the development of the medicinal value of H. suaveolens and have important theoretical significance for the chemical utilization of H. suaveolens resources.

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