scholarly journals Composition, Antimicrobial and Free-radical Scavenging Activities of the Essential Oil of Plectranthus marrubatus

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700
Author(s):  
Kaleab Asres ◽  
Solomon Tadesse ◽  
Avijit Mazumder ◽  
Franz Bucar
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Broad Spectrum ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Traditional Use ◽  
Fungal Strains ◽  
Free Radical Scavenging Activities

Essential oil from the aerial part of Plectranthus marrubatus J. K. Morton (Lamiaceae), obtained by hydrodistillation was analyzed by gas chromatography/mass spectrometry (GC/MS) and evaluated for antimicrobial and free radical scavenging activities. Twenty-four compounds representing 99% of the total oil were identified. The major constituents were thymol, p-cymene and γ-terpinene. The oil was tested against 21 bacterial and 4 fungal strains using the disc diffusion method and found to be active against a broad spectrum of pathogens including Gram-positive and Gram-negative bacteria as well as some fungal strains. The minimum inhibitory concentrations (MICs) of the oil against the bacterial strains tested ranged from 10 to 800 μg/mL, and from 400 to 800 μg/mL against the fungal strains employed. The in vitro antioxidant activity was assessed using 2,2-diphenyl-1-picrylhydrazil (DPPH) and showed a low EC50 value of 0.15 μl/mL. The study provides evidence for the broad-spectrum antimicrobial and antioxidant effect of Plectranthus marrubatus essential oil, and a possible explanation for its traditional use in the treatment of cold, fever, stomach disorder, diarrhea and as a skin cleaner.

scholarly journals Composition and biological assays of the leaf essential oil of Asparagus flagellaris (Kunth) Bak

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Oluwakayode O. Odeja ◽  
Michael Gabriel Ibok ◽  
Ejike O. Okpala
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Klebsiella Pneumonia ◽  
Leaf Essential Oil

Abstract Background Asparagus flagellaris leaves are ethnomedicinally used to treat syphilis, gonorrhea and other sexually transmitted diseases (STDs), with no reports on the volatile constituents. This study was aimed to quantitatively and qualitatively characterise the composition of essential oil, evaluates the free radical scavenging and antimicrobial capacity of the essential oil. Methods The essential oil was isolated by hydrodistillation method using all-glass Clevenger-type apparatus, while the identification and quantification of constituents were performed by gas chromatography-mass spectrometry (GC-MS) technique. The antioxidant activity on 2, 2-diphenyl-1-picrylhydrazyl (DPPH) was evaluated and the oil was also tested against 10 strains of microorganisms consisting of 6 bacteria: Escherichia coli, Salmonella typhi, Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis and 4 fungi: Candida albicans, Penicillium notatum, Aspergillus niger and Rhizopus spp. using broth dilution and surface plate methods, respectively. Results A pale yellow essential oil with a characteristic scent was obtained, with a yield of 0.80% (w/w). A total of 28 compounds accounting for 97.41% of the total oil contents were identified. The oil was predominated by Thymol and its derivatives, accounting for 57.48%. The most abundance (% area) constituents of the essential oil were 5-Thymyl tiglate (18.49%), Thymyl-2-methyl butyrate (17.34%), Thymol hydroquinone dimethyl ether (10.52%), Thymol methyl ether (9.42%) and 5-Propyl-1, 3-benzodioxole (4.59%). The essential oil showed a significant free radical scavenging activity compared to the standard antioxidant drugs used in this study, with % inhibition varying from 88.06 ± 0.0001 to 93.05 ± 0.0006. The leaf essential oil exhibited antimicrobial activity on all the tested organisms at 500–125 μg/mL, with an 18–10 mm inhibitory zone. Conclusion The leaf essential oil of A. flagellaris contains notable chemical compounds responsible for its antioxidant and antimicrobial activities.

scholarly journals Chemical Composition of the Essential Oil of Ligularia hodgsonii and Free Radical Scavenging Activity of the Oil and Crude Extracts

2014 ◽  
Vol 9 (10) ◽  
pp. 1934578X1400901
Author(s):  
Zihao Zhou ◽  
Jun Tang ◽  
Xueping Song
Keyword(s):  
Free Radical ◽  
Chemical Composition ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Total Oil ◽  
Lower Capacity ◽  
Free Radical Scavenging Activities

The chemical composition of the essential oil from roots and rhizomes of Ligularia hodgsonii was analyzed by gas chromatography (GC) and GC-mass spectrometry (MS). Forty-seven components were identified, representing 77.0% of the total oil. The main constituents were l-(+)-ascorbic acid 2,6-dihexadecanoate (15.7%), selina-6-en-4-ol (8.4%) and 9,10-dimethyl-1,2,3,4,5,6,7,8-octahydroanthracene (6.6%). The free radical scavenging activities of the essential oil, aqueous extract, ethanolic extract, and crude polysaccharides of L. hodgsonii, as well as some of their major components, were investigated using DPPH and ABTS assays. The essential oil displayed a lower capacity to quench free radicals than the extracts; the ethanolic and aqueous extracts showed considerably higher antioxidant potential that deserves further study.

Antimicrobial and free radical scavenging activities of crude extract and fractions from the tuberous root of Mirabilis jalapa L.

2012 ◽  
Vol 2 (5) ◽  
pp. 209-216
Author(s):  
J. Gogoi ◽  
K. S. Nakhuru ◽  
A. K. Rai ◽  
P. Chattophadhayay ◽  
H. K. Gogoi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Amyl Alcohol ◽  
Free Radical Scavenging ◽  
Tuberous Root ◽  
Diffusion Method ◽  
Mirabilis Jalapa ◽  
Free Radical Scavenging Activities

Mirabilis jalapa L. belonging to family Nyctaginaceae is a widely used traditionalmedicinal plant in different parts of the world. In present study, aqueous methanolic extract and fractions from tuberous root of M. jalapa were evaluated for antimicrobial and free radical scavenging activities. The tuberousroot was extracted in 80 % methanol. Phytochemical constituents were tested following standard procedures which revealed the presence of terpenoids, flavonoids, tannins, steroids, phenolic compounds and alkaloids. The dried extract was further fractionated by using solvents chloroform, ethyl acetate, amyl alcohol, methanol and water. Free radical scavenging capacity was evaluated by 2, 2 diphenyl 1 picrylhydrazine (DPPHô€ˆ©Ë™) and 2, 2’–azinobis (3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS+) assays. IC50 values of extractwere 60±0.71μg/ml and 80±0.56 μg/ml with DPPH and ABTS assays, respectively. Ethyl acetate and amyl alcohol fractions exhibited strong antioxidant activity among the fractions. Antimicrobial activity of extract and fractions was evaluated against selected human pathogens such as Staphylococcus aureus, Streptococcus mutans, Escherchia coli and Candida albicans by agar well diffusion method. The extract and fractions were most active against S. mutans and E. coli. The present study indicated that the root of M. jalapa possess potential antioxidant and broad spectrum antimicrobial activity which could be explored for pharmaceutical applications.

Free radical scavenging and antiacetylcholinesterase activities of Origanum majorana L. essential oil

2011 ◽  
Vol 30 (10) ◽  
pp. 1501-1513 ◽  
Author(s):  
A T H Mossa ◽  
GAM Nawwar
Keyword(s):  
Mass Spectrometry ◽  
Free Radical ◽  
Essential Oil ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Inhibitory Effects ◽  
Cholinesterase Inhibitory Activity ◽  
Origanum Majorana

In the present study, Origanum majorana L. essential oil (EO) was analyzed by gas chromatography-mass spectrometry (GC-MS) and evaluated for free radical scavenging and anticholinesterase activities. GC-MS analysis revealed the presence of 4-terpineol (29.97%), γ-terpinene (15.40%), trans-sabinene hydrate (10.93), α-terpinene (6.86%), 3-cycolohexene-1-1 methanal,a,a4-trimethyl-,(S)-(CAS) (6.54%), and sabinene (3.91%) as main constituents. Origanum majorana L. EO exhibited concentration-dependent inhibitory effects on 2,2′-diphenylpicrylhydrazyl (DPPH•), hydroxyl radical, hydrogen peroxide, reducing power, and lipid peroxidation with IC50 values of 58.67, 67.11, 91.25, 78.67, and 68.75 µg/mL, respectively; while the IC50 values for the standard trolox were noted to be 23.95, 44.97, 51.30, 42.22, and 52.72 µg/mL, respectively. Interestingly, cholinesterase inhibitory activity was also found with IC50 values of 36.40 µg/mL. We can conclude that the marjoram EO has a significant potential to be used as a natural antioxidant and anti-AChE.

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