Antimicrobial and free radical scavenging activities of crude extract and fractions from the tuberous root of Mirabilis jalapa L.

2012 ◽  
Vol 2 (5) ◽  
pp. 209-216
Author(s):  
J. Gogoi ◽  
K. S. Nakhuru ◽  
A. K. Rai ◽  
P. Chattophadhayay ◽  
H. K. Gogoi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Amyl Alcohol ◽  
Free Radical Scavenging ◽  
Tuberous Root ◽  
Diffusion Method ◽  
Mirabilis Jalapa ◽  
Free Radical Scavenging Activities

Mirabilis jalapa L. belonging to family Nyctaginaceae is a widely used traditionalmedicinal plant in different parts of the world. In present study, aqueous methanolic extract and fractions from tuberous root of M. jalapa were evaluated for antimicrobial and free radical scavenging activities. The tuberousroot was extracted in 80 % methanol. Phytochemical constituents were tested following standard procedures which revealed the presence of terpenoids, flavonoids, tannins, steroids, phenolic compounds and alkaloids. The dried extract was further fractionated by using solvents chloroform, ethyl acetate, amyl alcohol, methanol and water. Free radical scavenging capacity was evaluated by 2, 2 diphenyl 1 picrylhydrazine (DPPHô€ˆ©Ë™) and 2, 2’–azinobis (3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS+) assays. IC50 values of extractwere 60±0.71μg/ml and 80±0.56 μg/ml with DPPH and ABTS assays, respectively. Ethyl acetate and amyl alcohol fractions exhibited strong antioxidant activity among the fractions. Antimicrobial activity of extract and fractions was evaluated against selected human pathogens such as Staphylococcus aureus, Streptococcus mutans, Escherchia coli and Candida albicans by agar well diffusion method. The extract and fractions were most active against S. mutans and E. coli. The present study indicated that the root of M. jalapa possess potential antioxidant and broad spectrum antimicrobial activity which could be explored for pharmaceutical applications.

scholarly journals Phytochemical Composition and Biological Activities of Wild Scolymus maculatus L.

Medicines ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 53 ◽  
Author(s):  
Saleh Abu-Lafi ◽  
Mahmoud Rayan ◽  
Mahmud Masalha ◽  
Basheer Abu-Farich ◽  
Hashem Al-Jaas ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Salmonella Typhimurium ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Effective Concentration ◽  
Dpph Assay ◽  
Phytochemical Composition ◽  
Hexane Extracts

Background: The wild population of spotted golden thistle, Scolymus maculatus, which belongs to the Compositae family, is believed to be one of the multi-curative wild plants mentioned in Flora Palaestina. This study aims to disclose the phytochemical composition, antioxidant potential, and antimicrobial activity of wild S. maculatus collected from the farms of Kabul, a village in northwest Galilee, for the first time. Methods: The phytochemical components of crude S. maculatus extracts from methanol, ethyl acetate, and n-hexane solvents were separated and identified using gas chromatography-mass spectrometry (GC-MS) in the electron impact (EI) mode. The free radical scavenging of the plant extracts was measured by DPPH assay. The microdilution test was used to determine the minimum inhibitory concentrations (MICs) of different S. maculatus extracts and to evaluate their antimicrobial activities. Results: Thirty-two phytochemicals were found in S. maculatus extracts including stigmasterol, γ-sitosterol, lupeol, lupeol acetate, and β-amyrin. Phytochemicals, such as 2-linoleoylglycerol, γ-sitosterol, β-amyrin, lupeol, (3α)-12-oleanen-3-yl acetate, and lupenyl acetate, were found to dominate the methanol extract. Most of these compounds were also observed in ethyl acetate and n-hexane extracts, but at different levels, in addition to some other minor compounds. The various extracts were investigated for their antioxidant and antimicrobial activity. The ethanolic and the methanolic extracts were shown to exhibit the highest free radical scavenging by DPPH assay with a half-maximally effective concentration (EC50) of 0.37 and 0.65 mg/mL respectively, while the other three extracts (aqueous, ethyl acetate and n-hexane) were less active and their EC50 (effective concentration at which DPPH radical was scavenged by 50%) were above 1.0 mg/mL. Moreover, MICs were determined to be effective against Staphylococcus aureus, Salmonella typhimurium, and Candida albicans microorganisms. Ethyl acetate and the ethanolic extracts are active against the three types of microorganisms at a minimum inhibitory concentration (MIC) of 0.5 mg/mL, while aqueous and the n-hexane extracts are inactive against Salmonella typhimurium. Conclusions: The results show that S. maculatus extracts are a rich source of compounds that can play an important role in human health, and in a broader context, in the treatment of various diseases, such antimicrobial and antioxidant-related ailments.

scholarly journals BIOMIMETIC SYNTHESIS, CHARACTERIZATION AND EVALUATION OF ANTIOXIDANT, ANTIMICROBIAL EFFICACY OF SILVER NANOPARTICLES USING ANDREDERA CORDIFOLIA LEAF EXTRACT

2017 ◽  
Vol 10 (4) ◽  
pp. 329 ◽  
Author(s):  
Rajathi Palanivelrajan ◽  
Suja S
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Biomimetic Synthesis ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Frap Assay ◽  
Vis Spectroscopy

Objective: The present study is focussed on the biosynthesis of silver nanoparticles using aqueous extract of Andredera cordifolia and to investigate the free radical scavenging potential, antimicrobial activity of the nanoparticles against different human pathogens.Methods: The formation of silver nanoparticles was indicated by the colour change from colourless to reddish brown. Biosynthesized nanoparticles was characterised using several techniques, viz- UV-Vis spectroscopy, FT-IR, XRD, TEM, SEM and EDAX analysis. The free radical scavenging potential was measured by DPPH, FRAP assay, antimicrobial activity against six microorganisms was tested using disc diffusion method.  Results: UV-Vis spectral analysis showed silver Surface Plasmon Resonance band at 426 nm.  The crystalline morphology and size of the nanoparticles were determined by TEM, SEM, and X-ray diffraction studies which showed the average size of the nanoparticles in the range 40 –60nm.  The biologically synthesized nanoparticles efficiently inhibited pathogenic organisms such as E. coli, Staphylococcus aureus, Klebsiella Pneumonia, Pseudomonas aeruginosa and Proteus vulgaris.  The biosynthesized nanoparticles might serve as a potent antioxidant as revealed by DPPH assay and FRAP assay. Conclusion: The biosynthesis of silver nanoparticles had several advantages in pharmaceutical applications as well as large scale commercial production.Key Words: Silver nanoparticle, Andredera cordifolia leaf, SEM,TEM,XRD,antioxidant, antimicrobial,etc.

scholarly journals Chemical composition, antimicrobial and free radical scavenging activities of Grewia pubescens

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Fats And Oils ◽  
Free Radical Scavenging ◽  
Rhizopus Stolonifer ◽  
Penicillium Notatum ◽  
Methanol Extracts ◽  
Gas Chromatography Mass Spectroscopy ◽  
Free Radical Scavenging Activities

The phytochemical screening of n-hexane, ethyl acetate and methanol extracts of Grewia pubescens leaves showed the presence of alkaloids, steroids, terpenoids, flavonoids, saponins, anthraquinones, tannins, glycosides and fats and oils. The three extracts were tested on six bacterial and four fungal strains, and exhibited satisfactory inhibitory activities against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomona aeruginosa, Salmonella typhii, Klebsiellae pneumonae, Candida albicans, Aspergillus niger, penicillium notatum and Rhizopus stolonifer, except ethyl acetate extract which showed no antifungal property on penicillium notatum and Rhizopus stolonifer. Methanol extract of the plant possessed significant antioxidant activity by exhibiting DPPH free radical scavenging property with IC50 of 10.26 μM, using DPPH antioxidant assay. The compounds present in the extracts were characterised using Gas chromatography-Mass spectroscopy (GC-MS). Seven, three and six compounds were revealed in n-hexane, ethyl acetate and methanol extracts of Grewia pubescens leaves respectively, while hexadecanoic acid is the most abundant constituent in all three extracts with corresponding percentage of abundance of 47.92, 53.20 and 61.59.

scholarly journals Composition, Antimicrobial and Free-radical Scavenging Activities of the Essential Oil of Plectranthus marrubatus

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700
Author(s):  
Kaleab Asres ◽  
Solomon Tadesse ◽  
Avijit Mazumder ◽  
Franz Bucar
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Broad Spectrum ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Traditional Use ◽  
Fungal Strains ◽  
Free Radical Scavenging Activities

Essential oil from the aerial part of Plectranthus marrubatus J. K. Morton (Lamiaceae), obtained by hydrodistillation was analyzed by gas chromatography/mass spectrometry (GC/MS) and evaluated for antimicrobial and free radical scavenging activities. Twenty-four compounds representing 99% of the total oil were identified. The major constituents were thymol, p-cymene and γ-terpinene. The oil was tested against 21 bacterial and 4 fungal strains using the disc diffusion method and found to be active against a broad spectrum of pathogens including Gram-positive and Gram-negative bacteria as well as some fungal strains. The minimum inhibitory concentrations (MICs) of the oil against the bacterial strains tested ranged from 10 to 800 μg/mL, and from 400 to 800 μg/mL against the fungal strains employed. The in vitro antioxidant activity was assessed using 2,2-diphenyl-1-picrylhydrazil (DPPH) and showed a low EC50 value of 0.15 μl/mL. The study provides evidence for the broad-spectrum antimicrobial and antioxidant effect of Plectranthus marrubatus essential oil, and a possible explanation for its traditional use in the treatment of cold, fever, stomach disorder, diarrhea and as a skin cleaner.

Anti-Acne Inducing Bacteria and Free Radical Scavenging Activities of Turmeric Rhizome Extracts Prepared Using Different Solvents

2017 ◽  
Author(s):  
R Nurficahyanti ◽  
P Charoenwiwattanakij ◽  
R Wattanalai ◽  
W Pothitirat
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activities

Antioxidant and Free Radical Scavenging Activities of Culinary-Medicinal Mushrooms, Golden Chanterelle Cantharellus cibarius and Angel's Wings Pleurotus porrigens

2010 ◽  
Vol 12 (3) ◽  
pp. 265-272 ◽  
Author(s):  
Mohammad Ali Ebrahimzadeh ◽  
Seyed Mohammad Nabavi ◽  
Seyed Fazel Nabavi ◽  
Shahram Eslami
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Medicinal Mushrooms ◽  
Free Radical Scavenging Activities

MICROWAVE-ASSISTED SEED ESSENTIAL OIL OF ELEUTHEROCOCCUS SENTICOSUS AND ITS ANTIOXIDANT AND FREE RADICAL-SCAVENGING ACTIVITIES

2012 ◽  
Vol 37 (1) ◽  
pp. 119-127 ◽  
Author(s):  
VIVEK K. BAJPAI ◽  
AJAY SHARMA ◽  
SUNG HONG KIM ◽  
YONGHO KIM ◽  
JONG-JOO KIM ◽  
...  
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Eleutherococcus Senticosus ◽  
Microwave Assisted ◽  
Free Radical Scavenging Activities

scholarly journals On the free radical scavenging activities of melatonin's metabolites, AFMK and AMK

2012 ◽  
Vol 54 (3) ◽  
pp. 245-257 ◽  
Author(s):  
Annia Galano ◽  
Dun Xian Tan ◽  
Russel J. Reiter
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activities
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