Larvicidal Activity of Isoflavonoids from Muellera Frutescens Extracts Against Aedes Aegypti

The biological activity of extracts from the leaves, bark and roots of Muellera frutescens, an Amazonian ichtyotoxic plant, were evaluated to find new environmentally safe insecticides. The n-hexane extracts of bark, leaf, and root showed a strong toxic activity against Aedes aegypti mosquito larvae. Bioguided fractionation of the bark extract led to the isolation of seven isoflavonoids (12a-hydroxyelliptone, elliptone, (-)-variabilin, rotenone, rotenolone, tephrosin and deguelin). Rotenone and deguelin are responsible for the larvicidal activity of the plant. M. frutescens leaves contain up to 0.6%, w/w, deguelin. These results justify the traditional ichtyotoxic use of M. frutescens. The leaves contain a relatively high proportion of deguelin and, therefore, can be considered as a renewable source of this environmentally friendly insecticidal isoflavonoid.

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Synthesis and Biological Activity of Benzamides Substituted with Pyridine-Linked 1,2,4-Oxadiazole

Molecules ◽

10.3390/molecules25153500 ◽

2020 ◽

Vol 25 (15) ◽

pp. 3500

Author(s):

Sen Yang ◽

Xiao-Yu Tian ◽

Tian-Yang Ma ◽

Li Dai ◽

Chao-Li Ren ◽

...

Keyword(s):

Biological Activity ◽

High Activity ◽

1H Nmr ◽

Larvicidal Activity ◽

13C Nmr ◽

Inhibitory Activity ◽

Mosquito Larvae ◽

Lead Compounds ◽

Larvicidal Activities

To find pesticidal lead compounds with high activity, a series of novel benzamides substituted with pyridine-linked 1,2,4-oxadiazole were designed by bioisosterism, and synthesized easily via esterification, cyanation, cyclization and aminolysis reactions. The structures of the target compounds were confirmed by 1H-NMR, 13C-NMR and HRMS. The preliminary bioassay showed that most compounds had good larvicidal activities against mosquito larvae at 10 mg/L, especially compound 7a, with a larvicidal activity as high as 100%, and even at 1 mg/L was still 40%; at 50 mg/L, all the target compounds showed good fungicidal activities against the eight tested fungi. Moreover, compound 7h exhibited better inhibitory activity (90.5%) than fluxapyroxad (63.6%) against Botrytis cinereal. Therefore, this type of compound can be further studied.

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Larvicidal activity of aqueous extracts of Ilex paraguariensis and Ilex theezans on Aedes aegypti (L.)

Ciência e Natura ◽

10.5902/2179460x32377 ◽

2020 ◽

Vol 42 ◽

pp. e3

Author(s):

Ana Carla Knakiewicz ◽

Junir Antonio Lutinski ◽

Maria Assunta Busato ◽

Walter Antônio Roman Junior ◽

Daniel Albeny Simões

Keyword(s):

Aedes Aegypti ◽

Larvicidal Activity ◽

Zika Virus ◽

Ilex Paraguariensis ◽

Mosquito Larvae ◽

Yerba Mate ◽

Aqueous Extracts ◽

Plant Variation ◽

Chikungunya Fever ◽

Controlled Conditions

Aedes aegypti is the main vector of Dengue, Yellow Urban Fever, Chikungunya fever and Zika virus fever. The strategies for its control include synthetic products that cause damage to the environment and other organisms. This study evaluated the larvicidal activity of aqueous extracts of leaves and fruits of Ilex paraguariensis and Ilex theezans on Aedes aegypti mosquito larvae. The bioassays were conducted at the Ecological Entomology Laboratory, Unochapecó, under controlled conditions of temperature and photoperiod. The extracts of fresh and dried leaves of I. theezans showed greater larvicidal activity when compared to extracts of fruits of the same plant. Variation in larvicidal activity was also observed during exposure periods. The results suggest the use of extracts of these plants in the control of A. aegypti and the prospection of substances that can be used as an alternative to synthetic products. They point to the possibility of using yerba mate tissues that are not used commercially.

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Tephrosia Toxicaria Pers Essential Oil: Chemical Composition and Larvicidal Activity

Natural Product Communications ◽

10.1177/1934578x0600100509 ◽

2006 ◽

Vol 1 (5) ◽

pp. 1934578X0600100 ◽

Cited By ~ 1

Author(s):

Walber H. F. Ribeiro ◽

Jackson N. Vasconcelos ◽

Angela M. C. Arriaga ◽

Maria C. F. de Oliveira ◽

Manoel Andrade-Neto ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Aedes Aegypti ◽

Essential Oils ◽

Larvicidal Activity ◽

Mosquito Larvae ◽

Main Constituent ◽

Germacrene D

The essential oils obtained by hydrodistillation of the leaves, stems, and shoots of Tephrosia toxicaria Pers (Papilionaceae) were analyzed using GC and GC/MS. Germacrene D was the major component (20.7-33.3 %) of the oils of leaves and shoots, whereas spathulenol was the main constituent (42.3%) of the stem oil. The essential oils from the leaves and stems, when tested for activity against mosquito larvae (Aedes aegypti), gave LC 50 values of 81.3 ± 2.9 and 63.1 ± 1.0 ppm, respectively.

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Larvicidal Activity of some Bacterial Insecticides and Insect Growth Regulators against Mosquito Larvae of Aedes aegypti (L.)

Alexandria Science Exchange Journal An International Quarterly Journal of Science Agricultural Environments ◽

10.21608/asejaiqjsae.2014.2816 ◽

2014 ◽

Vol 35 (OCTOBER- DECEMBER) ◽

pp. 256-262

Keyword(s):

Aedes Aegypti ◽

Larvicidal Activity ◽

Growth Regulators ◽

Mosquito Larvae ◽

Insect Growth Regulators ◽

Insect Growth

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Larvicidal Properties of the Three Major Furostanol Saponins of Balanites aegyptiaca Fruit Mesocarp against Aedes agypti Mosquito Larvae

Natural Product Communications ◽

10.1177/1934578x0700200804 ◽

2007 ◽

Vol 2 (8) ◽

pp. 1934578X0700200

Author(s):

Bishnu P. Chapagain ◽

Zeev Wiesman

Keyword(s):

Nmr Spectroscopy ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Biological Activities ◽

Mosquito Larvae ◽

Balanites Aegyptiaca ◽

Methanol Extracts ◽

H Nmr ◽

Furostanol Saponins ◽

Oligosaccharide Chain

Among various biological activities, saponin-rich extracts have been found to be larvicidal against mosquito larvae; however, no study so far has reported using pure furostanol saponins. In this study, three major furostanol saponins: 26-( O-β-D-glucopyranosyl)-3β,22,26-trihydroxyfurost-5-ene 3- O-β-D-glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (1), 26-( O-β-D-glucopyranosyl)-22- O-methylfurost-5-ene-3β,26-diol 3- O-β-D-xylopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (2), and 26-( O-β-D-glucopyranosyl)-22- O-methylfurost-5-ene.3β,26-diol 3- O-β-D-glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (3) were isolated from methanol extracts of the fruit mesocarp of Balanites aegyptiaca (L.) Del. (desert date). Their identification was carried out by ESI-MSn and structures were elucidated by 800 MHz 1H NMR spectroscopy. The larvicidal properties of these saponins against the Aedes aegypti mosquito larvae were evaluated. Results suggest that all three saponins possess larvicidal activity, but the activity was found to be related to the structure of the saponins, basically in the C-22 position of the aglycone and in the oligosaccharide chain. A furostanol saponin that presents -OCH3 at the C-22 position compared to an -OH group, and the lack of a xylose molecule compared to its presence is found to be more larvicidal.

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Application of Renewable Kenaf Cellulose Nanofiber as a Temephos Nanocarrier for Control of Aedes Aegypti (Diptera: Culicidae) Larvae

10.21203/rs.3.rs-302304/v1 ◽

2021 ◽

Author(s):

Hazirah binti Pengiran ◽

Jahangir Kamaldin ◽

Leo Bey Fen ◽

Hamdan Ahmad

Keyword(s):

Aedes Aegypti ◽

High Performance ◽

Control Method ◽

Cellulose Nanofiber ◽

Effective Control ◽

Mosquito Larvae ◽

Renewable Source ◽

Transmission Electron ◽

Materials Used ◽

Microscopy Examination

Abstract Larviciding is an effective control method in managing mosquito-borne diseases. However, current carrier materials used in larvicides formulation raised environmental concerns due to its non-renewable origin. Herein, our study aimed to evaluate the application of the renewable source kenaf cellulose nanofiber (KCNF) impregnated with temephos for the control of Aedes aegypti mosquito larvae. The morphology of KCNF+T was examined using field emission scanning electron microscope (FESEM) and transmission electron microscopy (TEM) while the quantity of temephos impregnated, released, and retained on the fibers upon dispersion in water were determined using high performance liquid chromatography (HPLC). Subsequently, the bioefficacy of the KCNF+T was evaluated against Ae. aegypti larvae. The FESEM and TEM micrograph verified the presence of temephos on the KCNF after the impregnation. HPLC analysis showed the amount of temephos impregnated on KCNF was 97 % of the 0.1 mg temephos. Upon dispersion in water, KCNF+T released 53% temephos and the retention of temephos on KCNF+T gradually decreased to 30, 17 and 7 % on the first, third and fifth month, respectively. The exposure of Ae. aegypti larvae to KCNF+T at concentration 0.006 to 0.01 mg/L was effective at 17 - 25 folds in killing Ae. aegypti larvae compared to temephos without KCNF. Microscopy examination revealed accumulation of the KCNF on the larval appendages. Overall, our study demonstrated that KCNF made from renewable source is an effective nanocarrier of temephos for the control of Ae. aegypti mosquito larvae.

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Larvicidal Activity of Gliricidia sepium Against Mosquito Larvae of Anopheles stephansi, Aedes aegypti and Culex quinquefasciatus

Pharmaceutical Biology ◽

10.1076/phbi.36.1.3.4616 ◽

1998 ◽

Vol 36 (1) ◽

pp. 3-7 ◽

Cited By ~ 11

Author(s):

Nirmal Sharma ◽

J.S. Qadry ◽

B. Subramanium ◽

T. Verghese ◽

S.J. Rahman ◽

...

Keyword(s):

Aedes Aegypti ◽

Larvicidal Activity ◽

Culex Quinquefasciatus ◽

Gliricidia Sepium ◽

Mosquito Larvae

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Bioefficacy of Epaltes divaricata (L.) n-Hexane Extracts and Their Major Metabolites against the Lepidopteran Pests Spodoptera litura (fab.) and Dengue Mosquito Aedes aegypti (Linn.)

Molecules ◽

10.3390/molecules26123695 ◽

2021 ◽

Vol 26 (12) ◽

pp. 3695

Author(s):

Kesavan Amala ◽

Sengodan Karthi ◽

Raja Ganesan ◽

Narayanaswamy Radhakrishnan ◽

Kumaraswamy Srinivasan ◽

...

Keyword(s):

Aedes Aegypti ◽

Larvicidal Activity ◽

Spodoptera Litura ◽

Insect Pests ◽

Bioactive Metabolites ◽

Dependent Manner ◽

Hexadecanoic Acid ◽

Chemical Screening ◽

Hexane Extracts ◽

Dose Dependent

The present research investigated the chemical characterization and insecticidal activity of n-Hexane extracts of Epaltes divaricata (NH-EDx) along with their chief derivatives n-Hexadecanoic acid (n-HDa) and n-Octadecanoic acid (n-ODa) against the dengue vector Aedes aegypti and lepidopteran pest Spodoptera litura. Chemical screening of NH-EDx through GC–MS analysis delivered nine major derivatives, and the maximum peak area percentage was observed in n-Hexadecanoic acid (14.63%) followed by n-Octadecadienoic acid (6.73%). The larvicidal activity of NH-EDx (1000 ppm), n-HDa (5 ppm), and n-ODa (5 ppm) against the A. aegypti and S. litura larvae showed significant mortality rate in a dose-dependent way across all the instars. The larvicidal activity was profound in the A. aegypti as compared to the S. litura across all the larval instars. The sublethal dosages of NH-EDx (500 ppm), n-HDa (2.5 ppm), and n-ODa (2.5 ppm) also showed alterations in the larval/pupal durations and adult longevity in both the insect pests. The enzyme activity revealed that the α- and β-carboxylesterase levels were decreased significantly in both the insect pests, whereas the levels of GST and CYP450 uplifted in a dose-dependent manner of NH-EDx, n-HDa, and n-ODa. Correspondingly, midgut tissues such as the epithelial layer (EL), gut lumen (GL), peritrophic matrix (Pm), and brush border membrane (BBM) were significantly altered in their morphology across both A. aegypti and S. litura against the NH-EDx and their bioactive metabolites. NH-EDx and their bioactive metabolites n-HDa and n-ODa showed significant larvicidal, growth retardant, enzyme inhibition, and midgut toxicity effects against two crucial agriculturally and medically challenging insect pest of ecological importance.

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