scholarly journals Phytochemical, Antimicrobial and Antiplasmodial Investigations of Terminalia brownii

2013 ◽  
Vol 8 (6) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Francis Machumi ◽  
Jacob O. Midiwo ◽  
Melissa R. Jacob ◽  
Shabana I. Khan ◽  
Babu L. Tekwani ◽  
...  
Keyword(s):  
Ellagic Acid ◽  
Soluble Fraction ◽  
2D Nmr ◽  
Stem Bark ◽  
Bark Extract ◽  
Spectroscopic Methods ◽  
Group 4 ◽  
Phytochemical Investigation ◽  
Stem Bark Extract ◽  
Ellagic Acid Derivatives

Phytochemical investigation of the ethyl acetate-soluble fraction of stem bark extract of an African medicinal plant Terminalia brownii led to the isolation of a new oleanane-type triterpenoid, along with seven known triterpenoids, seven ellagic acid derivatives, and 3- O-β-D-glucopyranosyl-β-sitosterol. The new compound was identified using spectroscopic methods, notably 1D- and 2D NMR, as 3β,24- O-ethylidenyl-2α,19α-dihydroxyolean-12-en-28-oic acid. The isolated compounds were evaluated for their antimicrobial and antiplasmodial activities. Two compounds with a galloyl group (4 and 6) were found to be active against chloroquine sensitive (D6) and chloroquine resistant (W2) strains of Plasmodium falciparum, whereas three ellagic acid derivatives (5–7) were found active against three species of fungi and one species of bacteria.

scholarly journals Isolation of Epicatechin from the Stem Bark of Neocarya macrophylla (Sabine) Prance (Chrysobalanaceae)

2020 ◽  
Vol 27 (2) ◽  
pp. 101-107
Author(s):  
A.J. Yusuf ◽  
M.I. Abdullahi ◽  
A.M. Musa ◽  
A.K. Haruna ◽  
V. Mzozoyana ◽  
...  
Keyword(s):  
Soluble Fraction ◽  
2D Nmr ◽  
Stem Bark ◽  
Bark Extract ◽  
2D Nmr Spectroscopy ◽  
Chemical Test ◽  
Medicinal Uses ◽  
Wide Range ◽  
Stem Bark Extract ◽  
Existing Data

Neocarya macrophylla has a wide range of medicinal uses in traditional medicine. The aim of the study was to isolate and characterize compound from the stem bark of the plant. (-)-Epicatechin (a flavan-3-ol) was isolated from the ethylacetate soluble fraction of the methanol stem bark extract of the plant using a combination of silica gel and sephadex LH-20 column chromatography. The structure of the compound as (-)-epicatechin was confirmed on the basis of chemical test, 1D- & 2D-NMR spectroscopy and comparison with existing data in literature. This is the first report of isolation of epicatechin from the stem bark of the plant. Keywords: Neocarya macrophylla, stem bark, (-)-Epicatechin, NMR analysis

Antimicrobial triterpenes from the stem bark of Crossopteryx febrifuga

2015 ◽  
Vol 70 (7-8) ◽  
pp. 169-173 ◽  
Author(s):  
Jean Rodolphe Chouna ◽  
Jean-de-Dieu Tamokou ◽  
Pépin Nkeng-Efouet-Alango ◽  
Bruno Ndjakou Lenta ◽  
Norbert Sewald
Keyword(s):  
Candida Parapsilosis ◽  
Enterobacter Aerogenes ◽  
Stem Bark ◽  
Bark Extract ◽  
Dioic Acid ◽  
Antibacterial And Antifungal Activities ◽  
Phytochemical Investigation ◽  
Stem Bark Extract ◽  
Antibacterial And Antifungal

Abstract Phytochemical investigation of the stem bark extract of Crossopteryx febrifuga resulted in the isolation of epimeric mixtures of 3β-urs-12,20(30)-diene-27,28-dioic acid and 18-epi-3β-urs-12,20(30)-diene-27,28-dioic acid (1), as well as: 3β-D-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid and 18-epi-3β-D-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid (2), together with some known compounds such as the monoglyceride of palmitic acid, as well as β-sitosterol and its glucoside. The structures of the isolated compounds were determined by application of spectroscopic methods. The MeOH extract and compounds 1 and 2 were examined for antimicrobial activity in in vitro assays against bacteria (Enterobacter aerogenes ATCC13048, Escherichia coli ATCC8739, Klebsiella pneumoniae ATCC11296, Staphylococcus aureus) and fungi (Candida parapsilosis, Candida albicans ATCC 9002 and Cryptococcus neoformans IP 90526). The tested samples showed selective activities. The antibacterial and antifungal activities of compound 2 (MIC=8–64 μg/mL) were in some cases equal to or even higher than those of the respective reference drugs chloramphenicol (MIC=16– 64 μg/mL) and nystatin (MIC=128–256 μg/mL).

scholarly journals Phytochemical Investigation and Anti-Microbial Activity of Clausena anisata on Cariogenic and Periodontopathic Bacteria

2020 ◽  
pp. 11-25
Author(s):  
Ashu Michael Agbor ◽  
Kuete Pierre Fotsing ◽  
Suzy Hermine Dountio ◽  
Ntungwen Charles Fokunang ◽  
Sudeshni Naidoo
Keyword(s):  
Antibacterial Activity ◽  
Public Health Problem ◽  
Fusobacterium Nucleatum ◽  
Stem Bark ◽  
Bark Extract ◽  
Major Public Health Problem ◽  
Bacteriostatic Activity ◽  
Oral Infections ◽  
Phytochemical Investigation ◽  
Stem Bark Extract

Clausen aanisata is used in African traditional medicine to treat a variety of infectious and parasitic diseases. Infectious diseases are a major public health problem especially in developing countries where resistance to commonly used antibiotics is growing exponentially. The objective of this study was to evaluate the antibacterial activity of Clausena anisata on cariogenic and periodonthopatic bacteria. This was a laboratory experimental study using macerated hydroethanolic extracts of the stem and leaves of Clausena anisata in a water-ethanol mixture (30:70) for 48 hours. Phytochemical screening revealed flavonoids, tannins, alkaloids, coumarins, phenols, triterpenes and saponosides, these being more abundant in the leaves. The antibacterial activity of the hydroethanolic extracts was evaluated against different clinically isolated strains of three Gram negative (Aggregatibacter actinomycetemcomitan, Fusobacterium nucleatum and Prevotella intermedia) and two Gram positive (Streptococcus mutans and Latobacillusspp) bacteria. Polyphenols found more in the leaves extract was estimated at 62.8 mgTAE/g against 36.8 mgTAE/g in the stem bark extract. The study revealed that the leaves extract had bactericidal and bacteriostatic activity on isolated strains of Aggregatibacter actinomycetemcomitan and Fusobacterium nucleatum being the most susceptible with MICs of 50 mg/ml whereas the stem bark showed bacteriostatic activity on all isolated strains with Aggregatibacter actinomycetemcomitan being the most susceptible with MIC of 50 mg/ml. The antimicrobial action of the plant could be exploited in formulation of mouth washes and other medications in the management of oral infections like periodontitis and tooth dacay.

scholarly journals Stigmasterol and Stigmasterone from Methanol Extract of Calophyllum soulattri Burm. F. Stem Bark

2021 ◽  
Vol 24 (4) ◽  
pp. 108-113
Author(s):  
Surya Dewi Marliyana ◽  
Fajar Rakhman Wibowo ◽  
Desi Suci Handayani ◽  
Triana Kusumaningsih ◽  
Venty Suryanti ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Methanol Extract ◽  
Biological Activities ◽  
Stem Bark ◽  
Bark Extract ◽  
Solvent Fractionation ◽  
Phytochemical Investigation ◽  
Stem Bark Extract ◽  
Flash Column Chromatography ◽  
Vacuum Liquid Chromatography

Stigmasterol and Stigmasterone from Methanol Extract of Calophyllum soulattri Burm. F. Stem Bark. Calophyllum soulattri Burm. F. has been widely used for herbal medicine. Phytochemical investigation of C. soulattri contains a secondary metabolite of the steroid class. Steroid compounds have various biological activities, such as anti-inflammatory, antioxidant, antiproliferative, antibacterial, antimalarial, and anticancer. Two secondary metabolites steroids have been isolated and identified from the stem bark extract of C. soulattri. Isolation was carried out through the extraction (maceration), fractionation, and purification stages. Maceration is carried out using methanol as a solvent. Fractionation was carried out by vacuum liquid chromatography (VLC), and purification was by flash column chromatography. Identification of combined fractions and determination of pure isolates were used through thin-layer chromatography (TLC). The solvent used in the chromatography methods was a mixture of n-hexane and ethyl acetate. The structure isolates were identified by FTIR, 1H NMR, and 13C NMR and compared with literature data. Secondary metabolites steroids that have been isolated are identical compounds to stigmasterol and stigmasterone.

Cytotoxic and antimicrobial activities of phytoconstituents of the stem bark extract of Psychotria djumaensis

2021 ◽  
Vol 14 (2) ◽  
pp. 11-15
Author(s):  
Mbasso Joseph Eric Tameko ◽  
Ngansop Raymond Nono ◽  
Marcel Frese ◽  
Jean Rodolphe Chouna ◽  
Ndjakou Bruno Lenta ◽  
...  
Keyword(s):  
Stem Bark ◽  
Antimicrobial Activities ◽  
Bark Extract ◽  
Cervix Carcinoma ◽  
Pentacyclic Triterpenoids ◽  
Phytochemical Investigation ◽  
Stem Bark Extract ◽  
Reported Data ◽  
Weak Antibacterial Activity ◽  
Human Cervix

Phytochemical investigation of the stem bark extract of Psychotria djumaensis led to the isolation of ten known pentacyclic triterpenoids among which one lupane-, three oleanane-,  and six ursane-types. The structures of the isolated compounds were established by means of spectroscopic methods and by comparison with previously reported data. The isolated compounds were tested against five strains of bacteria, and the cytotoxicity against the human cervix carcinoma KB-3-1 cell was evaluated. The isolates exhibited weak antibacterial activity against the five strains. The cytotoxic activity exhibited by 3β,19α-dihydroxyurs-12-en-28-oic acid (5) (IC50 5.9 µM) was about three times more significant than that of the reference [(+) griseofulvin, IC50 17-21 µM].

scholarly journals Antibacterial Screening and Phytochemical investigation of bark extracts of Acacia jacquemontii Benth.

2010 ◽  
Vol 2 (2) ◽  
pp. 21-26
Author(s):  
K Choudhary ◽  
M Singh ◽  
U Pillai ◽  
N S Shekhawat
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extract ◽  
Antimicrobial Agents ◽  
Stem Bark ◽  
Diffusion Method ◽  
Bark Extract ◽  
Pharmaceutical Sciences ◽  
Antibacterial Screening ◽  
Phytochemical Investigation ◽  
Stem Bark Extract

Acacia  jacquemontii was  assessed  for  active  principles  to  ascertain  the  rationale  for  its  use  in  traditional medicine. Preliminary phytochemical screening of the stem bark extracts showed that it possessed the active principles - alkaloids, glycosides, saponins, terpenoids and tannins. The antimicrobial activity of the extracts was assayed against pathogenic strains of Bacillus cereus, Bacillus pumilus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, S. pyrogenes, and Candida albcans using  the agar diffusion method. The plant extract exhibited antimicrobial activity against all the test microorganisms. B. cereus and B. pumilus were the most susceptible to the plant extract while Candida albicans was the most resistant. The minimum inhibitory  concentration  of  the  stem  bark  extract  of  the  plant  ranged  between  30  and  50 mg/ml while  the minimum bactericidal concentration ranged between 35 and 60 mg/ml. A. jacquemontii could be a potential source of antimicrobial agents.   Key words: Antibacterial; Antifungal; Baonli; Medicinal plants.  DOI: 10.3329/sjps.v2i2.2384Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 21-26

Antibacterial secotirucallane triterpenes from the stem bark of Pseudocedrela kotschyi

2018 ◽  
Vol 73 (5-6) ◽  
pp. 241-246 ◽  
Author(s):  
Christèle Sorèle Mambou ◽  
Raymond Ngansop Nono ◽  
Jean Rodolphe Chouna ◽  
Jean-de-Dieu Tamokou ◽  
Pépin Nkeng-Efouet-Alango ◽  
...  
Keyword(s):  
2D Nmr ◽  
Stem Bark ◽  
Bark Extract ◽  
Gram Negative Bacteria ◽  
2D Nmr Spectroscopy ◽  
Dioic Acid ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Stem Bark Extract

AbstractThe antibacterial-guided investigation of the stem bark extract ofPseudocedrela kotschyiled to the isolation of a new secotirucallane triterpene derivative: 4-hydroxy-3,4-secotirucalla-7,24-dien-3,21-dioic acid (1), together with the known one: 3,4-secotirucalla-4(28),7,24-trien-3,21-dioic acid (2) and 3-methyl ester 3,4-secotirucalla-4(28),7,24-trien-3,21-dioic (3). The structures of the isolated compounds were elucidated on the basis of extensive 1D- and 2D-NMR spectroscopy. Extracts, fractions and compounds (1–3) were tested in vitro for antibacterial activity against two Gram positive bacteria (Bacillus subtilisandStaphylococcus aureusATCC 25923), and two Gram negative bacteria (Escherichia coliS2(1) andPseudomonas aeruginosa). The MeOH extract and the Hex/CH2Cl2(70:30) fraction showed significant levels of activity (MIC=64– 256 μg/mL) compared with the two reference drugs [ciprofloxacin: MIC (0.5–1 μg/mL) and amoxicillin: MIC (1–128 μg/mL)]. Moreover, the compound2isolated from this Hex/CH2Cl2(70:30) fraction had the greatest potential value againstS. aureus,E. coliandP. aeruginosa, with minimum inhibitory concentrations (MIC) ranging from 4–16 μg/mL.

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