Antibacterial secotirucallane triterpenes from the stem bark of Pseudocedrela kotschyi

AbstractThe antibacterial-guided investigation of the stem bark extract ofPseudocedrela kotschyiled to the isolation of a new secotirucallane triterpene derivative: 4-hydroxy-3,4-secotirucalla-7,24-dien-3,21-dioic acid (1), together with the known one: 3,4-secotirucalla-4(28),7,24-trien-3,21-dioic acid (2) and 3-methyl ester 3,4-secotirucalla-4(28),7,24-trien-3,21-dioic (3). The structures of the isolated compounds were elucidated on the basis of extensive 1D- and 2D-NMR spectroscopy. Extracts, fractions and compounds (1–3) were tested in vitro for antibacterial activity against two Gram positive bacteria (Bacillus subtilisandStaphylococcus aureusATCC 25923), and two Gram negative bacteria (Escherichia coliS2(1) andPseudomonas aeruginosa). The MeOH extract and the Hex/CH2Cl2(70:30) fraction showed significant levels of activity (MIC=64– 256 μg/mL) compared with the two reference drugs [ciprofloxacin: MIC (0.5–1 μg/mL) and amoxicillin: MIC (1–128 μg/mL)]. Moreover, the compound2isolated from this Hex/CH2Cl2(70:30) fraction had the greatest potential value againstS. aureus,E. coliandP. aeruginosa, with minimum inhibitory concentrations (MIC) ranging from 4–16 μg/mL.

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Antimicrobial triterpenes from the stem bark of Crossopteryx febrifuga

Zeitschrift für Naturforschung C ◽

10.1515/znc-2014-4168 ◽

2015 ◽

Vol 70 (7-8) ◽

pp. 169-173 ◽

Cited By ~ 6

Author(s):

Jean Rodolphe Chouna ◽

Jean-de-Dieu Tamokou ◽

Pépin Nkeng-Efouet-Alango ◽

Bruno Ndjakou Lenta ◽

Norbert Sewald

Keyword(s):

Candida Parapsilosis ◽

Enterobacter Aerogenes ◽

Stem Bark ◽

Bark Extract ◽

Dioic Acid ◽

Antibacterial And Antifungal Activities ◽

Phytochemical Investigation ◽

Stem Bark Extract ◽

Antibacterial And Antifungal

Abstract Phytochemical investigation of the stem bark extract of Crossopteryx febrifuga resulted in the isolation of epimeric mixtures of 3β-urs-12,20(30)-diene-27,28-dioic acid and 18-epi-3β-urs-12,20(30)-diene-27,28-dioic acid (1), as well as: 3β-D-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid and 18-epi-3β-D-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid (2), together with some known compounds such as the monoglyceride of palmitic acid, as well as β-sitosterol and its glucoside. The structures of the isolated compounds were determined by application of spectroscopic methods. The MeOH extract and compounds 1 and 2 were examined for antimicrobial activity in in vitro assays against bacteria (Enterobacter aerogenes ATCC13048, Escherichia coli ATCC8739, Klebsiella pneumoniae ATCC11296, Staphylococcus aureus) and fungi (Candida parapsilosis, Candida albicans ATCC 9002 and Cryptococcus neoformans IP 90526). The tested samples showed selective activities. The antibacterial and antifungal activities of compound 2 (MIC=8–64 μg/mL) were in some cases equal to or even higher than those of the respective reference drugs chloramphenicol (MIC=16– 64 μg/mL) and nystatin (MIC=128–256 μg/mL).

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Isolation of Epicatechin from the Stem Bark of Neocarya macrophylla (Sabine) Prance (Chrysobalanaceae)

Nigerian Journal of Basic and Applied Sciences ◽

10.4314/njbas.v27i2.14 ◽

2020 ◽

Vol 27 (2) ◽

pp. 101-107

Author(s):

A.J. Yusuf ◽

M.I. Abdullahi ◽

A.M. Musa ◽

A.K. Haruna ◽

V. Mzozoyana ◽

...

Keyword(s):

Soluble Fraction ◽

2D Nmr ◽

Stem Bark ◽

Bark Extract ◽

2D Nmr Spectroscopy ◽

Chemical Test ◽

Medicinal Uses ◽

Wide Range ◽

Stem Bark Extract ◽

Existing Data

Neocarya macrophylla has a wide range of medicinal uses in traditional medicine. The aim of the study was to isolate and characterize compound from the stem bark of the plant. (-)-Epicatechin (a flavan-3-ol) was isolated from the ethylacetate soluble fraction of the methanol stem bark extract of the plant using a combination of silica gel and sephadex LH-20 column chromatography. The structure of the compound as (-)-epicatechin was confirmed on the basis of chemical test, 1D- & 2D-NMR spectroscopy and comparison with existing data in literature. This is the first report of isolation of epicatechin from the stem bark of the plant. Keywords: Neocarya macrophylla, stem bark, (-)-Epicatechin, NMR analysis

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Spondias purpurea L. Stem Bark Extract: Antioxidant and in vitro Photoprotective Activities

Journal of the Brazilian Chemical Society ◽

10.21577/0103-5053.20210082 ◽

2021 ◽

Author(s):

Francisco Alessandro Rodrigues ◽

Priscylla Giffony ◽

Sarah dos Santos ◽

Jhonyson Guedes ◽

Maria Elenir Ribeiro ◽

...

Keyword(s):

Active Ingredient ◽

Antioxidant Activities ◽

Stem Bark ◽

Ultra Performance Liquid Chromatography ◽

Bark Extract ◽

Protection Factor ◽

Phenolic Constituents ◽

Stem Bark Extract ◽

Spondias Purpurea

Plant phenolic extracts are widely recognized as an important source of natural antioxidant substances and potential compounds for cosmetic formulations. This study aimed to evaluate the chemical profile, photoprotective and antioxidant activities of stem bark extract of Spondias purpurea L. (ciriguela) and its application in photoprotective formulations. Thirty phenolic constituents were annotated by ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry in mode negative (UPLC‑QTOF-MS2). The stem bark extract antioxidant and chelation potential, expressed in half maximal inhibitory concentration (IC50), showed 6.25 and 352.22 μg mL−1, respectively. The phenolic extract was used as an active ingredient in six sunscreen formulations, with concentrations ranging from 0.2 to 10%. The ultraviolet (UV) protection properties of the formulations were evaluated by sun protection factor (SPF) values obtained in 0.2 mg mL−1 (0.495 to 2.27) and 2.0 mg mL−1 (2.29 to 15.87). The SPF value for the extract (14.37 and 26.16) was high, but there was a reduction in the base formulation. However, these results suggested that the bioactive extracted of stem bark of Spondias purpurea L. has interesting potential to reduce the damage caused by UV radiation and may be utilized as an active ingredient in a sunscreen formulation.

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Antibacterial Potential of the Methanol Stem Bark Extract of Stachytarpheta Indica

Asian Journal of Medical Sciences ◽

10.3126/ajms.v4i4.8248 ◽

2013 ◽

Vol 4 (4) ◽

pp. 5-10

Author(s):

Christian Godwin Akuodor ◽

Monday Pius Udia ◽

Charles Emeka Udenze ◽

John Onyemaechi Ogbonna

Keyword(s):

Antimicrobial Agents ◽

Medical Science ◽

Stem Bark ◽

Bark Extract ◽

Significant Activity ◽

E Coli ◽

Diffusion Technique ◽

Shigella Spp ◽

Test Organisms ◽

Stem Bark Extract

Objective: There is increasing need for potent antimicrobial agents to tackle the problem of diseases in man. In view of this, the activities of methanol stem bark extract of Stachytarpheta indica was evaluated against some disease causing microorganisms. Method: The activity of the extract against S. aureus, P.aeruginosa, E. coli, S. typhi and Shigella spp was determined using agar diffusion technique. Results: The methanol stem bark extract demonstrated significant activity against the test organisms. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the stem bark extract ranged from 12-60 μg/ml. Conclusion: Further isolation of active compound responsible for the antibacterial activity could be the potential sources of new antibacterial agents. Asian Journal of Medical Science, Volume-4 (2013), Pages 5-10 DOI: http://dx.doi.org/10.3126/ajms.v4i4.8248

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Bioactive Compounds and in vitro Antimicrobial Activities of Ethanol Stem Bark Extract of Trilepisium madagascariense DC.

International Journal of Pharmacology ◽

10.3923/ijp.2018.901.912 ◽

2018 ◽

Vol 14 (7) ◽

pp. 901-912 ◽

Cited By ~ 2

Author(s):

Olufunmiso Olusola Olajuyigbe ◽

Tolulope Esther Onibudo ◽

Roger Murugas Coopoosamy ◽

Anofi Omotayo Tom Ashafa ◽

Anthony Jide Afolayan

Keyword(s):

Bioactive Compounds ◽

Stem Bark ◽

Antimicrobial Activities ◽

Bark Extract ◽

Stem Bark Extract

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Lack of in vitro antihyaluronidase activity of methanolic leaf extract of Indigofera tinctoria L and methanolic stem bark extract of Stereospermum suaveolens DC

Journal of Pharmaceutical Negative Results ◽

10.4103/0976-9234.157391 ◽

2015 ◽

Vol 6 (1) ◽

pp. 40

Author(s):

WanigasekaraDaya Ratnasooriya ◽

ChathurangaBharathee Ranaweera ◽

WalimuniPrabhashini Abeysekara ◽

Ranjith Pathirana

Keyword(s):

Leaf Extract ◽

Stem Bark ◽

Bark Extract ◽

Stem Bark Extract ◽

Indigofera Tinctoria

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ANTI-INFLAMMATORY ACTIVITY OF KNEMA ATTENUATA (HOOK. F. AND THOMSON) WARB ETHANOLIC STEM BARK EXTRACT IN ALBINO WISTAR RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i4.30500 ◽

2019 ◽

pp. 330-334

Author(s):

SUPRIYA RAJA H

Keyword(s):

Stem Bark ◽

Inflammatory Activity ◽

Assay Method ◽

Bark Extract ◽

Raw 264.7 ◽

Myeloperoxidase Activity ◽

Stem Bark Extract ◽

Anti Inflammatory

Objective: Knema attenuata (Myristicaceae), popularly known as “wild nutmeg,” is an endemic tree species from Western Ghats, which has been used in folk medicine. Conventionally, the stem bark of K. attenuata is used for treating inflammatory conditions without any scientific information available for the same. The present study was undertaken to evaluate the anti-inflammatory activity of the ethanolic stem bark extract (ESBE) of K. attenuata using in vivo and in vitro screening models. Methods: The ethanolic extract of stem bark was prepared by soxhlation, and its cytotoxicity in RAW 264.7 cell line was assessed using MTT assay method. In vivo anti-inflammatory effect of extract was estimated in rats using carrageenan-induced paw edema model and cotton pellet-induced granuloma model. The in vitro anti-inflammatory activity of the extract was evaluated by cyclooxygenase and lipoxygenase inhibition assay, estimation of myeloperoxidase activity, and determination of cellular nitrite levels in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Results: Toxic symptoms were not observed for the ESBE. The extract demonstrated significant anti-inflammatory activity in both in vivo and in vitro models. The anti-inflammatory action exhibited by the extract was a result of the inhibition of leukocyte migration and nitric oxide pathway and partially by inhibition of mediators such as prostaglandins and leukotrienes. Conclusion: Findings from the study provide the evidence for the popular use of stem bark extract of K. attenuata as a potential anti-inflammatory agent.

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