Isolation of Epicatechin from the Stem Bark of <i>Neocarya macrophylla</i> (Sabine) Prance (Chrysobalanaceae)

Neocarya macrophylla has a wide range of medicinal uses in traditional medicine. The aim of the study was to isolate and characterize compound from the stem bark of the plant. (-)-Epicatechin (a flavan-3-ol) was isolated from the ethylacetate soluble fraction of the methanol stem bark extract of the plant using a combination of silica gel and sephadex LH-20 column chromatography. The structure of the compound as (-)-epicatechin was confirmed on the basis of chemical test, 1D- & 2D-NMR spectroscopy and comparison with existing data in literature. This is the first report of isolation of epicatechin from the stem bark of the plant. Keywords: Neocarya macrophylla, stem bark, (-)-Epicatechin, NMR analysis

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Phytochemical, Antimicrobial and Antiplasmodial Investigations of Terminalia brownii

Natural Product Communications ◽

10.1177/1934578x1300800619 ◽

2013 ◽

Vol 8 (6) ◽

pp. 1934578X1300800 ◽

Cited By ~ 5

Author(s):

Francis Machumi ◽

Jacob O. Midiwo ◽

Melissa R. Jacob ◽

Shabana I. Khan ◽

Babu L. Tekwani ◽

...

Keyword(s):

Ellagic Acid ◽

Soluble Fraction ◽

2D Nmr ◽

Stem Bark ◽

Bark Extract ◽

Spectroscopic Methods ◽

Group 4 ◽

Phytochemical Investigation ◽

Stem Bark Extract ◽

Ellagic Acid Derivatives

Phytochemical investigation of the ethyl acetate-soluble fraction of stem bark extract of an African medicinal plant Terminalia brownii led to the isolation of a new oleanane-type triterpenoid, along with seven known triterpenoids, seven ellagic acid derivatives, and 3- O-β-D-glucopyranosyl-β-sitosterol. The new compound was identified using spectroscopic methods, notably 1D- and 2D NMR, as 3β,24- O-ethylidenyl-2α,19α-dihydroxyolean-12-en-28-oic acid. The isolated compounds were evaluated for their antimicrobial and antiplasmodial activities. Two compounds with a galloyl group (4 and 6) were found to be active against chloroquine sensitive (D6) and chloroquine resistant (W2) strains of Plasmodium falciparum, whereas three ellagic acid derivatives (5–7) were found active against three species of fungi and one species of bacteria.

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Antibacterial secotirucallane triterpenes from the stem bark of Pseudocedrela kotschyi

Zeitschrift für Naturforschung C ◽

10.1515/znc-2017-0207 ◽

2018 ◽

Vol 73 (5-6) ◽

pp. 241-246 ◽

Cited By ~ 2

Author(s):

Christèle Sorèle Mambou ◽

Raymond Ngansop Nono ◽

Jean Rodolphe Chouna ◽

Jean-de-Dieu Tamokou ◽

Pépin Nkeng-Efouet-Alango ◽

...

Keyword(s):

2D Nmr ◽

Stem Bark ◽

Bark Extract ◽

Gram Negative Bacteria ◽

2D Nmr Spectroscopy ◽

Dioic Acid ◽

E Coli ◽

Gram Positive Bacteria ◽

Stem Bark Extract

AbstractThe antibacterial-guided investigation of the stem bark extract ofPseudocedrela kotschyiled to the isolation of a new secotirucallane triterpene derivative: 4-hydroxy-3,4-secotirucalla-7,24-dien-3,21-dioic acid (1), together with the known one: 3,4-secotirucalla-4(28),7,24-trien-3,21-dioic acid (2) and 3-methyl ester 3,4-secotirucalla-4(28),7,24-trien-3,21-dioic (3). The structures of the isolated compounds were elucidated on the basis of extensive 1D- and 2D-NMR spectroscopy. Extracts, fractions and compounds (1–3) were tested in vitro for antibacterial activity against two Gram positive bacteria (Bacillus subtilisandStaphylococcus aureusATCC 25923), and two Gram negative bacteria (Escherichia coliS2(1) andPseudomonas aeruginosa). The MeOH extract and the Hex/CH2Cl2(70:30) fraction showed significant levels of activity (MIC=64– 256 μg/mL) compared with the two reference drugs [ciprofloxacin: MIC (0.5–1 μg/mL) and amoxicillin: MIC (1–128 μg/mL)]. Moreover, the compound2isolated from this Hex/CH2Cl2(70:30) fraction had the greatest potential value againstS. aureus,E. coliandP. aeruginosa, with minimum inhibitory concentrations (MIC) ranging from 4–16 μg/mL.

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Antimicrobial activity and phytochemistry of methanolic stem bark extract of Melia azedarach (Linn.)

Bayero Journal of Pure and Applied Sciences ◽

10.4314/bajopas.v12i1.61s ◽

2020 ◽

Vol 12 (1) ◽

pp. 407-411

Author(s):

M. Ahmed ◽

A.H. Bukar ◽

M.Y. Amuda ◽

A. Babagana

Keyword(s):

Antimicrobial Activity ◽

Developing Countries ◽

Traditional Medicine ◽

Stem Bark ◽

Bark Extract ◽

Melia Azedarach ◽

Human Pathogens ◽

Zone Of Inhibition ◽

Medicinal Uses ◽

Stem Bark Extract

The use of traditional medicine is increasing and gaining popularity throughout the developed and developing countries. About 80 % of the people in developing countries rely on traditional medicine for primary health care. The present study was carried out to evaluate the phytochemical constituents and antimicrobial activity of the stem bark of Melia azedarach extracted using methanol as solvent. Phytochemical screening revealed that methanolic stem bark extract contain alkaloid, cardiac glycoside, flavonoids, saponins, steroid, tannin and terpenoid. Antimicrobial efficiency were tested against four human pathogens: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Streptococcus spp. The diameter of zone of inhibition (ZOI) was measured and it was found that the extract showed a maximum zone of inhibition against Gram negative and Gram positive pathogens and the zone of inhibition was found to be concentration dependent. The result obtained in this study may be indication that alcoholic extracts of this plant could be a possible source of obtaining new and effective herbal medicine to treat infections which justify the ethno medicinal uses of Melia azedarach against infectious diseases agents. Key words: Melia azedarach, antimicrobial, activity, traditional medicine, extract

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Antioxidant and hepatoprotective potentials of miliusa velutina stem bark extract

Science and Technology Development Journal - Natural Sciences ◽

10.32508/stdjns.v4i3.880 ◽

2020 ◽

Vol 4 (3) ◽

pp. First

Author(s):

Đái Thị Xuân Trang ◽

Hieu Le Trung Bui ◽

Linh Chi Tran ◽

Danh Thai Luu ◽

Tuan Trong Nguyen

Keyword(s):

Antioxidant Activity ◽

Body Weight ◽

Ethanol Extract ◽

Stem Bark ◽

Hepatoprotective Effect ◽

Bark Extract ◽

Control Group ◽

Antioxidant Power ◽

Medicinal Uses ◽

Stem Bark Extract

Miliusa velutina (MV) stem bark has various medicinal uses, but its hepatoprotective effect has not yet been studied. This study investigated the antioxidant and hepatoprotective activity of the ethanol extract of MV stem bark against carbon tetrachloride (CCl4)-induced liver injury. The ethanol extract of MV stem bark was evaluated for in vitro antioxidant activity which exhibited good antioxidant activity in terms of ferric reducing-antioxidant power assay (EC50, FRAP=4.04±0.00 µg/mL), total antioxidant capacity assay (EC50, TAC=8.73±1.08 µg/mL) and 1,1-diphenyl-2-picrylhydrazyl (EC50, DPPH=9.33±0.07 µg/mL) radical scavenging assay. Mice were pretreated with CCl4 (2.5 mL/kg body wight per day) in 4 consecutive weeks. After one hour taking CCl4 by oral administration, mice were treated with the ethanol stem bark extract of MV at various concentrations of 100, 200, and 400 mg/kg body weight. The MV stem bark at the dose of 400 mg/kg body weight effectively reduced the level of alanine transaminase (38±6.78 U/L) and aspartate transaminase (AST) in serum. Besides, the MV stem bark at the dose 400 mg/kg body weight reduced the malondialdehyde (3.12±1,19 nM MDA/g tissue) level, and increased the activity of reduced glutathione (896.21±22.69 nM GSH/g tissue) in liver. The observation of the microscopic cross section of liver tissue also revealed that the mice treated with stem bark extract of MV at the dose of 200 and 400 mg/kg body weight had significantly improvement in liver tissues compared to the non-treated control group. Histological analyses of the MV-treated group exhibited reducing inflammatory process and preventing liver necrosis and fibrosis. In summary, the hepatoprotective effect of MV stem bark was seemingly associated with its antioxidant activity.

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