Cytotoxic and antimicrobial activities of phytoconstituents of the stem bark extract of Psychotria djumaensis

2021 ◽  
Vol 14 (2) ◽  
pp. 11-15
Author(s):  
Mbasso Joseph Eric Tameko ◽  
Ngansop Raymond Nono ◽  
Marcel Frese ◽  
Jean Rodolphe Chouna ◽  
Ndjakou Bruno Lenta ◽  
...  
Keyword(s):  
Stem Bark ◽  
Antimicrobial Activities ◽  
Bark Extract ◽  
Cervix Carcinoma ◽  
Pentacyclic Triterpenoids ◽  
Phytochemical Investigation ◽  
Stem Bark Extract ◽  
Reported Data ◽  
Weak Antibacterial Activity ◽  
Human Cervix

Phytochemical investigation of the stem bark extract of Psychotria djumaensis led to the isolation of ten known pentacyclic triterpenoids among which one lupane-, three oleanane-,  and six ursane-types. The structures of the isolated compounds were established by means of spectroscopic methods and by comparison with previously reported data. The isolated compounds were tested against five strains of bacteria, and the cytotoxicity against the human cervix carcinoma KB-3-1 cell was evaluated. The isolates exhibited weak antibacterial activity against the five strains. The cytotoxic activity exhibited by 3β,19α-dihydroxyurs-12-en-28-oic acid (5) (IC50 5.9 µM) was about three times more significant than that of the reference [(+) griseofulvin, IC50 17-21 µM].

Dialiumoside, an Olean-18-ene Triterpenoid from Dialium excelsum

2011 ◽  
Vol 66 (6) ◽  
pp. 624-628 ◽  
Author(s):  
Angelbert F. Awantu ◽  
Bruno N. Lenta ◽  
Tobias Bogner ◽  
Yanick F. Fongang ◽  
Silvère Ngouela ◽  
...  
Keyword(s):  
Biological Activity ◽  
Cytotoxic Activity ◽  
Stem Bark ◽  
Spectroscopic Methods ◽  
Cervix Carcinoma ◽  
Drug Resistant ◽  
Cytotoxicity Assays ◽  
Phytochemical Investigation ◽  
Reported Data ◽  
Human Cervix

Phytochemical investigation of the stem bark and fruits of Dialium excelsum led to the isolation of a new triterpenoid, named dialiumoside (1), together with twelve known compounds (2 - 13). The structure of the new compound as well as those of the known compounds were established by means of spectroscopic methods and by comparison with previously reported data. Compounds 1 - 13 were tested for their cytotoxic activity against the human cervix carcinoma KB-3-1 cells and the related multi drug-resistant P-gp-expressing KB-V1 cells. Compounds 1 and 13 showed weak biological activity in cytotoxicity assays while other compounds were inactive.

Bioactive Compounds and in vitro Antimicrobial Activities of Ethanol Stem Bark Extract of Trilepisium madagascariense DC.

2018 ◽  
Vol 14 (7) ◽  
pp. 901-912 ◽  
Author(s):  
Olufunmiso Olusola Olajuyigbe ◽  
Tolulope Esther Onibudo ◽  
Roger Murugas Coopoosamy ◽  
Anofi Omotayo Tom Ashafa ◽  
Anthony Jide Afolayan
Keyword(s):  
Bioactive Compounds ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Bark Extract ◽  
Stem Bark Extract

scholarly journals Evaluation of Antimicrobial Activities of Garcinia latissima Miq. Stem Bark Extract

2017 ◽  
Vol 9 (1s) ◽  
pp. s56-s59 ◽  
Author(s):  
Neneng Siti Silfi Ambarwati ◽  
Berna Elya ◽  
Amarila Malik ◽  
Muhamad Hanafi
Keyword(s):  
Stem Bark ◽  
Antimicrobial Activities ◽  
Bark Extract ◽  
Stem Bark Extract

Antimicrobial triterpenes from the stem bark of Crossopteryx febrifuga

2015 ◽  
Vol 70 (7-8) ◽  
pp. 169-173 ◽  
Author(s):  
Jean Rodolphe Chouna ◽  
Jean-de-Dieu Tamokou ◽  
Pépin Nkeng-Efouet-Alango ◽  
Bruno Ndjakou Lenta ◽  
Norbert Sewald
Keyword(s):  
Candida Parapsilosis ◽  
Enterobacter Aerogenes ◽  
Stem Bark ◽  
Bark Extract ◽  
Dioic Acid ◽  
Antibacterial And Antifungal Activities ◽  
Phytochemical Investigation ◽  
Stem Bark Extract ◽  
Antibacterial And Antifungal

Abstract Phytochemical investigation of the stem bark extract of Crossopteryx febrifuga resulted in the isolation of epimeric mixtures of 3β-urs-12,20(30)-diene-27,28-dioic acid and 18-epi-3β-urs-12,20(30)-diene-27,28-dioic acid (1), as well as: 3β-D-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid and 18-epi-3β-D-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid (2), together with some known compounds such as the monoglyceride of palmitic acid, as well as β-sitosterol and its glucoside. The structures of the isolated compounds were determined by application of spectroscopic methods. The MeOH extract and compounds 1 and 2 were examined for antimicrobial activity in in vitro assays against bacteria (Enterobacter aerogenes ATCC13048, Escherichia coli ATCC8739, Klebsiella pneumoniae ATCC11296, Staphylococcus aureus) and fungi (Candida parapsilosis, Candida albicans ATCC 9002 and Cryptococcus neoformans IP 90526). The tested samples showed selective activities. The antibacterial and antifungal activities of compound 2 (MIC=8–64 μg/mL) were in some cases equal to or even higher than those of the respective reference drugs chloramphenicol (MIC=16– 64 μg/mL) and nystatin (MIC=128–256 μg/mL).

scholarly journals Antimicrobial activities of extracts from Urtica urens

Food Research ◽  
2020 ◽  
Vol 4 (5) ◽  
pp. 1487-1492
Author(s):  
M.K. Pillai ◽  
R.P. Matamane ◽  
S.B. Mekbib
Keyword(s):  
Escherichia Coli ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Bark Extract ◽  
Root Extract ◽  
Fungal Isolates ◽  
Stem Bark Extract ◽  
Urtica Urens

The antibacterial and antifungal activities of various extracts from Urtica urens were evaluated against selected microorganisms using agar hole-plate diffusion method. A total of ten extracts namely U. urens hexane leaf extract (HLE), chloroform leaf extract (CLE), ethyl acetate leaf extract (ELE), methanolic leaf extract (MLE), hexane stem-bark extract (HSB), chloroform stem-bark extract (CSB), ethyl acetate stem-bark extract (ESB), methanolic stem-bark extract (MSB), chloroform root extract (CRT) and methanolic root extract (MRT) were prepared. These extracts were evaluated against two Gram-positive bacteria viz. Listeria monocytogenes and Staphylococcus aureus, four Gram-negative bacteria viz. Serratia marcescens, Pseudomonas aeruginosa, Escherichia coli (wild strain) and Escherichia coli (O157:H7) and two fungal isolates viz. Candida albicans and Penicillium digitatum. The inhibition zones of these extracts were found to be in the range of 9.0±0.0 to 40.5±0.7 mm against bacterial isolates and 16.0±4.2 to 17.5±4.9 mm against C. albicans. However, all these extracts exhibited no visible zone of inhibition against P. digitatum. Additionally, the minimum inhibitory concentrations (MICs) of these extracts were also determined and were found to be in the range of <31.25 to >1000 µg/mL against both bacterial and fungal isolates. From this study, we concluded that ELS, MLE, CRT and MRT showed promising antimicrobial activities.

scholarly journals Phytochemical Screening and Antimicrobial Activities of Lannea acida (a. rich) Stem Bark Extract

2020 ◽  
pp. 21-26
Author(s):  
O. A. Olatunji ◽  
C. I. Ihediuche ◽  
O. W. Bolaji ◽  
A. O. Akala ◽  
S. E. Edet ◽  
...  
Keyword(s):  
Cardiac Glycosides ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Bark Extract ◽  
Klebsiella Pneumonia ◽  
Continuous Extraction ◽  
Antimicrobial Drugs ◽  
Stem Bark Extract ◽  
Plant Stem ◽  
Micro Organisms

The persistent resistance of micro-organisms necessitates the need to intensify studies on the use of possible strategies for proper control of pathogens using extracts from natural products. The stem bark of Lannea acida was extracted by two methods of extraction: Cold and Soxhlet (continuous) extraction. The extract of the stem bark was screened to confirm the presence of alkaloids, flavonoids, saponins, tannins and cardiac glycosides in all the samples of ethanol and hexane/acetone/methanol mixture extracts. The phytochemicals examined varied among the stem bark extracts of other solvents (hexane, chloroform, acetone, and methanol). The result of the antimicrobial test shows that the stem bark extracts exhibited efficacy against some microbes (Bacillus cereus, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa Staphylococcus aureus and Streptococcus pyogenes) that were employed for the study by exhibiting clear inhibition/clearance zones against these microbes which ranges from 06 mm -21 mm. The plant stem bark could therefore be seen as a potential natural source for useful antimicrobial drugs.

Antibacterial effects of black mulberry (Morus nigra) stem bark extract on Streptococcus mutans

2021 ◽  
pp. 4399-4402
Author(s):  
Diah Lia Aulifa ◽  
Sakinah Haque ◽  
Hesti Riasari ◽  
Arif Budiman
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Morphological Changes ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Bark Extract ◽  
Ion Leakage ◽  
Bacterial Cells ◽  
Morus Nigra ◽  
Stem Bark Extract

Secondary metabolite compounds from Morus nigra (MN), has been shown to have antioxidant and antimicrobial activities. moreover, phenolics and flavonoids contained in this species are partly responsible for those activities. This study aims to evaluate the mode of action of MN stem bark extract and its antibacterial activity against Streptococcus mutans. The stem bark of MN was macerated using ethanol as a solvent for 72 h. Furthermore, the antibacterial activities of the extract were evaluated using Agar diffusion and microdilution methods, by determining the zone of inhibition and the minimum inhibitory concentration (MIC). Scanning electron microscopy (SEM) was used to observe the morphological changes induced by the extract. The protein and ion leakage from the bacterial cells were analyzed spectrophotometrically. The extract exhibited antibacterial activity against Streptococcus mutans, at a MIC value of 8mg/mL. Furthermore, it discovered via SEM that at 4xMIC, this extract could damage the membrane cell of Streptococcus mutans. Finally, protein and ion leakage were observed in bacterial cells of Streptococcus mutans induced with this extract. MN stem bark extract is a potential herbal medicine, which has antibacterial activity against Streptococcus mutans.

scholarly journals Antimicrobial activities of extracts from stem bark of Tagetes minuta

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2089-2094
Author(s):  
M.K. Pillai ◽  
L.I. Santi ◽  
S.B. Mekbib
Keyword(s):  
Escherichia Coli ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bark Extract ◽  
Bacterial Isolates ◽  
Tagetes Minuta ◽  
Food And Beverage ◽  
Stem Bark Extract

Tagetes minuta hexane stem bark extract (TMHESB), chloroform stem bark extract (TMCHSB), ethyl acetate stem bark extract (TMEASB) and methanolic stem bark extract (TMMESB) were evaluated for their antimicrobial activities using hole-plate diffusion method. Six bacterial isolates viz. Staphylococcus aureus, Listeria monocytogenes, Escherichia coli (wild), Escherichia coli (O157:H7), Pseudomonas aeruginosa and Serratia marcescens and two fungal isolates viz. Candida albicans and Penicillium digitatum were used for this study. The inhibition zones were found to be in the ranges of 10.0±1.6 to 15.5±1.9 mm against bacterial isolates and 11.3±2.1 to 13.4±1.2 mm against P. digitatum. However, these extracts did not exhibit any visible inhibition zone against C. albicans. Additionally, the minimum inhibitory concentrations (MICs) of these extracts were also determined and was found to be in the range of <31.25 to 1000 µg/mL. From this study, we concluded that extracts of the stem bark of T. minuta showed a moderate to significant antimicrobial activities. T. minuta has been used in food and beverage industries as preservative, coloring and flavoring agents. T. minuta also finds therapeutic applications in the traditional medicine.

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