scholarly journals Direct Identification and Characterization of Phenolic Compounds from Crude Extracts of Buds and Internodes of Grapevine (Vitis vinifera cv Merlot)

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901
Author(s):  
Said Qsaib ◽  
Nuno Mateus ◽  
Fatima Ez-zohra Ikbal ◽  
Lala Aicha Rifai ◽  
Victor de Freitas ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
Vitis Vinifera L ◽  
Reverse Phase Hplc ◽  
Direct Identification ◽  
Array Detection ◽  
First Time ◽  
Identification And Characterization

The crude methanol extracts of latent buds and internodes Vitis vinifera L. cv. Merlot were used for the determination of phenolic compounds by a combination of reverse phase HPLC with diode array detection (HPLC-DAD) and mass spectrometry (LC-MS). This method allowed the identification of 9 phenolic compounds without purification or fractionation. These 9 compounds were divided into three groups: procyanidins, flavonols and stilbenes. Detection by HPLC-DAD at different wave lengths of 280 nm to 320 nm, allowed the estimation of concentrations of those compounds. This method permitted, for the first time, both characterization and quantification of polyphenolic compounds in buds of grapevine. Comparison with the results obtained in internodes showed that quercetin, resveratrol tetramer and ε-viniferin had similar levels in buds and internodes while six other compounds identified had higher levels in buds.

scholarly journals Genome-wide identification and characterization of the metal tolerance protein (MTP) family in grape (Vitis vinifera L.)

3 Biotech ◽  
2019 ◽  
Vol 9 (5) ◽  
Author(s):  
Zahra Shirazi ◽  
Amin Abedi ◽  
Mojtaba Kordrostami ◽  
David J. Burritt ◽  
Mohammad Anwar Hossain
Keyword(s):  
Vitis Vinifera ◽  
Metal Tolerance ◽  
Vitis Vinifera L ◽  
Genome Wide ◽  
Metal Tolerance Protein ◽  
Identification And Characterization

Determination of Phenolic Compounds in the Methanolic Extract of Pourouma guianensis Leaves by UPLC-DAD-MS

2020 ◽  
Vol 10 (5) ◽  
pp. 566-570
Author(s):  
Queitilane de S. Sales ◽  
Marcelo da S. Mathias ◽  
Rodrigo R. de Oliveira
Keyword(s):  
Phenolic Compounds ◽  
Formic Acid ◽  
Solvent System ◽  
Efficient Tool ◽  
Phenolic Substances ◽  
Chemical Knowledge ◽  
First Time ◽  
Southeastern Region

Background: The presence of phenolic compounds is common in Urticaceae. However, only one report of the isolation of an apigenin in the Pourouma genus is available. Pourouma guianensis occurs widely throughout Brazil, mainly in the Southeastern region, and is popularly known as “pitinga”. The chemical profile of P. guianensis is marked by the presence of steroids and triterpenes. Objective: This study carried out the characterization of phenolic substances using UPLC-DAD-MS separation, in order to increase the chemical knowledge on the Pourouma genus. Methods: The extract was analyzed by UPLC-DAD-MS using a C-18 column, DAD detector at 190- 400nm and ESI-Q-TOF mass spectrometer. The solvent system consisted of water with 0.1% formic acid (phase A) and acetonitrile with 0.1% formic acid (phase B). Results: The extract analysis identified the presence of ten compounds: two aryl propanoids (5-Ocaffeoylquinic acid and 3-O-feruloylquinic acid) and eight glycosylated flavones derived from apigenin, luteonin, and chrysoeryol (vicenin 2, saponarin, orientin, isoorientin, isoschaftoside, schaftoside, isovitexin, and isoscoparin). Conclusion: The developed UPLC-DAD method was proven to be an efficient tool for the separation of phenolic mixtures and the UPLC-DAD-MS analysis enabled the identification of these compounds, reported for the first time in Pourouma genus.

scholarly journals Molecular cloning and characterization of a grapevine (Vitis vinifera L.) serotonin N-acetyltransferase (VvSNAT2) gene involved in plant defense

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Yihe Yu ◽  
Lu Bian ◽  
Zeling Jiao ◽  
Keke Yu ◽  
Yutong Wan ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Vitis Vinifera ◽  
Plant Defense ◽  
Marker Genes ◽  
Vitis Vinifera L ◽  
Melatonin Treatment ◽  
Gene Encoding ◽  
Ja Signaling ◽  
First Time

Abstract Background Melatonin is a ubiquitous molecule and exists across kingdoms. Studies on melatonin in plants have mainly focused on its physiological influence on growth and development, and on its biosynthesis. A number of studies have been conducted on the melatonin content and exogenous melatonin treatment of grapevine (Vitis vinifera L.). However, key genes or enzymes of the melatonin biosynthetic pathway remain unclear. Results In this study, we cloned and identified the gene encoding serotonin N-acetyltransferase (SNAT) in grapevine (VvSNAT2). The VvSNAT2 protein was identified from a collection of 30 members of the grapevine GCN5-related N-acetyltransferase (GNAT) superfamily. Phylogenetic and protein sublocalization analyses showed that the candidate gene VvGNAT16 is VvSNAT2. Characterization of VvSNAT2 showed that its enzymatic activity is highest at a pH of 8.8 and a temperature of 45 °C. Analysis of enzyme kinetics showed the values of Km and Vmax of VvSNAT2 using serotonin were 392.5 μM and 836 pmol/min/mg protein, respectively. The expression of VvSNAT2 was induced by melatonin treatment and pathogen inoculation. Overexpression of VvSNAT2 in Arabidopsis resulted in greater accumulation of melatonin and chlorophyll and enhanced resistance to powdery mildew in the transgenic plants compared with the wild type (WT). Additionally, our data showed that the marker genes in the salicylic acid (SA) signaling pathway were expressed to higher levels in the transgenic plants compared with the WT. Conclusions The VvSNAT2 gene was cloned and identified in grapevine for the first time. Our results indicate that VvSNAT2 overexpression activates the SA and JA signaling pathways; however, the SA pathway plays a central role in VvSNAT2-mediated plant defense.

scholarly journals Characterization of Yew Tree (Taxus) Varieties by Fingerprint and Principal Component Analyses

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700
Author(s):  
Kalle Truus ◽  
Merike Vaher ◽  
Maria Borissova ◽  
Marju Robal ◽  
Tuuli Levandi ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Principal Component ◽  
Baccatin Iii ◽  
Peak Areas ◽  
Array Detection ◽  
Using Data ◽  
Yew Tree

The composition of varieties of Taxus growing in Estonia was analyzed by capillary electrophoresis with diode array detection (CE-DAD) for the separation of phenolic compounds, and by high performance liquid chromatography-mass spectrometry (HPLC-MS) for the determination of taxoids. The main purpose of this study was the chemotaxonomic differentiation of varieties of Taxus by using data from these analyses. Fingerprints scanned at 214 nm on the basis of CE separation at pH 9.3 were used to characterize seven varieties of yew. The contents of four key taxoids (10-deacetylbaccatin, baccatin III, cephalomannine and paclitaxel) in six Taxus varieties were comparatively determined by HPLC-MS. The set of electropherograms/chromatograms of the various Taxus extracts were subjected to principal component analysis (PCA), using the peak areas of 16 phenolic compounds and 14 taxoids as characteristics. The formation of distinct clusters in accordance with botanical classification proves the suitability of PCA for differentiating varieties of Taxus.

scholarly journals Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv. Syrah grape pomace extract

2019 ◽  
Vol 10 (4) ◽  
pp. 1856-1869 ◽  
Author(s):  
Joana R. Costa ◽  
Manuela Amorim ◽  
Ana Vilas-Boas ◽  
Renata V. Tonon ◽  
Lourdes M. C. Cabral ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
Human Health ◽  
Grape Pomace ◽  
Vitis Vinifera L ◽  
Gastrointestinal Digestion ◽  
Gastrointestinal Health ◽  
Bioactive Properties

Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including in gastrointestinal health.

Rootstock effects on grape anthocyanins, skin and seed proanthocyanidins and wine color and phenolic compounds from Vitis vinifera L. Merlot grapevines

2019 ◽  
Vol 99 (6) ◽  
pp. 2846-2854 ◽  
Author(s):  
Gastón Gutiérrez‐Gamboa ◽  
Encarna Gómez‐Plaza ◽  
Ana B Bautista‐Ortín ◽  
Teresa Garde‐Cerdán ◽  
Yerko Moreno‐Simunovic ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
Vitis Vinifera L ◽  
Wine Color
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