scholarly journals Diterpenoid Constituents of Teucrium scordium L. Subsp. scordioides (Shreb.) Maire Et Petitmengin

2020 ◽  
Vol 15 (9) ◽  
pp. 1934578X2095952
Author(s):  
Petko I. Bozov ◽  
Plamen N. Penchev ◽  
Tania D. Girova ◽  
Velizar K. Gochev
Keyword(s):  
Mass Spectrometry ◽  
Acetone Extract ◽  
Ionization Mass ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Aerial Parts ◽  
Phytochemical Investigation ◽  
Nmr Techniques ◽  
Clerodane Diterpenoids ◽  
C Nmr

A new scordidesin А (1) and 2 known furo-clerodane diterpenoids, teucrin A (2) and 6-ketoteuscordin (3), were isolated by the phytochemical investigation of the acetone extract from the aerial parts of Teucrium scordium L. subsp. scordioides (Lamiaceae). The structure and stereochemistry of the compounds were established by high-resolution electrospray ionization mass spectrometry, infrared spectroscopy, and various 1H nuclear magnetic resonance (NMR) and 2-dimensional-NMR techniques. Additionally, we report 13C NMR spectral data of 3 that were not published before. Antimicrobial activity of the compounds was tested against Gram-positive and Gram-negative bacteria belonging to 5 different species and against 2 strains of yeast belonging to 2 different species of the genus Candida.

Glycosides from Cephalaria Species

2010 ◽  
Vol 65 (11) ◽  
pp. 1384-1392 ◽  
Author(s):  
Erkan Halay ◽  
Süheyla Kırmızıgül
Keyword(s):  
Mass Spectrometry ◽  
Oleanolic Acid ◽  
2D Nmr ◽  
Spectroscopic Methods ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Nmr Techniques ◽  
Basic Hydrolysis ◽  
Hydrolysis Of ◽  
Chemical Evidence

Three novel triterpene glycosides (1 - 3), namely lycicoside I, II and cilicicoside I, were isolated from two different Cephalaria (Dipsacaceae) species along with one known oleanane- and one iridoit- type of glycoside. The structures of these compounds were established as 3-O-[β -D-glucopyranosyl( 1→3)-α-L-rhamnopyranosyl(1→4)-β -D-xylopyranosyl(1→4)-β -D-xylopyranosyl]-28-O-[β -Dglucopyranosyl( 1→6)-β -D-glucopyranosyl]-oleanolic acid (1), 3-O-[β -D-xylopyranosyl(1→3)-α- L-rhamnopyranosyl(1→4)-β -D-xylopyranosyl]-28-O-[β -D-glucopyranosyl]-oleanolic acid (2) from Cephalaria lycica Matthew and 3-O-{β -D-glucopyranosyl(1→4)-β -D-xylopyranosyl(1→3)-α-Lrhamnopyranosyl( 1→2)-[β -D-glucopyranosyl(1→3)]-α -L-rhamnopyranosyl}-28-O-[β -D-glucopyranosyl( 1→6)-β -D-glucopyranosyl]-hederagenin (3) from Cephalaria cilicica Boiss. & Kotschy, on the basis of spectroscopic methods (1D and 2D NMR techniques, mass spectrometry) and chemical evidence. In addition, three new prosapogenins, 1B - 3B, were obtained from the basic hydrolysis of 1 - 3. The antimicrobial activity of 1 - 3 was tested against some Gram-positive and Gram-negative bacteria strains.

A dihydrochalcone derivative and further steroidal saponins from Sansevieria trifasciata Prain

2017 ◽  
Vol 72 (11-12) ◽  
pp. 477-482 ◽  
Author(s):  
Billy T. Tchegnitegni ◽  
Rémy B. Teponno ◽  
Kristina Jenett-Siems ◽  
Matthias F. Melzig ◽  
Tomofumi Miyamoto ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Spectroscopic Analysis ◽  
Ionization Mass ◽  
Steroidal Saponins ◽  
Chemical Methods ◽  
Aerial Parts ◽  
Phytochemical Investigation ◽  
Aconitic Acid ◽  
Related Compounds

AbstractPhytochemical investigation of the aerial parts ofSansevieria trifasciata, one of the most common Dracaenaceae plants, has resulted in the isolation of a new dihydrochalcone derivative named trifasciatine C (1), four previously unreported steroidal saponins as two pairs of inseparable regioisomers: trifasciatosides K/L (2/3), M/N (4/5), together with the known 1,2-(dipalmitoyl)-3-O-β-D-galactopyranosylglycerol (6), aconitic acid (7), and 1-methyl aconitic acid (8). Their structures were elucidated mainly by extensive spectroscopic analysis (1D and 2D nuclear magnetic resonance) and high-resolution electronspray ionization-mass spectrometry, as well as chemical methods and comparison of their spectral data with those of related compounds. Compounds2/3and4/5were evaluated for their antiproliferative activity on Hela cells, and no significant effect was observed.

scholarly journals Performance of PCR/Electrospray Ionization-Mass Spectrometry on Whole Blood for Detection of Bloodstream Microorganisms in Patients with Suspected Sepsis

2020 ◽  
Vol 58 (9) ◽  
Author(s):  
Kristoffer Strålin ◽  
Richard E. Rothman ◽  
Volkan Özenci ◽  
Kieron Barkataki ◽  
David Brealey ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Molecular Diagnostics ◽  
Whole Blood ◽  
Antimicrobial Treatment ◽  
Ionization Mass ◽  
Gram Negative Bacteria ◽  
Suspected Sepsis ◽  
Gram Negative ◽  
Esi Ms

ABSTRACT Blood culture (BC) often fails to detect bloodstream microorganisms in sepsis. However, molecular diagnostics hold great potential. The molecular method PCR/electrospray ionization-mass spectrometry (PCR/ESI-MS) can detect DNA from hundreds of different microorganisms in whole blood. The aim of the present study was to evaluate the performance of this method in a multicenter study including 16 teaching hospitals in the United States (n = 13) and Europe (n = 3). First, on testing of 2,754 contrived whole blood samples, with or without spiked microorganisms, PCR/ESI-MS produced 99.1% true-positive and 97.2% true-negative results. Second, among 1,460 patients with suspected sepsis (sepsis-2 definition), BC and PCR/ESI-MS on whole blood were positive in 14.6% and 25.6% of cases, respectively, with the following result combinations: BC positive and PCR/ESI-MS negative, 4.3%; BC positive and PCR/ESI-MS positive, 10.3%; BC negative and PCR/ESI-MS positive, 15.3%; and BC negative and PCR/ESI-MS negative, 70.1%. Compared with BC, PCR/ESI-MS showed the following sensitivities (coagulase-negative staphylococci not included): Gram-positive bacteria, 58%; Gram-negative bacteria, 78%; and Candida species, 83%. The specificities were >94% for all individual species. Patients who had received prior antimicrobial medications (n = 603) had significantly higher PCR/ESI-MS positivity rates than patients without prior antimicrobial treatment—31% versus 22% (P < 0.0001)—with pronounced differences for Gram-negative bacteria and Candida species. In conclusion, PCR/ESI-MS showed excellent performance on contrived samples. On clinical samples, it showed high specificities, moderately high sensitivities for Gram-negative bacteria and Candida species, and elevated positivity rates during antimicrobial treatment. These promising results encourage further development of molecular diagnostics to be used with whole blood for detection of bloodstream microorganisms in sepsis.

Flavon- and Flavonolglycosides from Achillea pannonica Scheele

2001 ◽  
Vol 56 (7-8) ◽  
pp. 521-525 ◽  
Author(s):  
Denata Kasaj ◽  
Liselotte Krenn ◽  
Sonja Prinz ◽  
Antje Hüfner ◽  
Shi Shan Yuc ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
2D Nmr ◽  
Flavonoid Glycosides ◽  
Spectroscopic Methods ◽  
Esi Ms ◽  
Aerial Parts ◽  
Nmr Techniques ◽  
Chemotaxonomic Study ◽  
First Time ◽  
C Nmr

The detailed investigation of a methanolic extract of aerial parts of Achillea pannonica SCHEELE. within a chemotaxonomic study led to the isolation of 6 flavonoid glycosides. Besides rutin, apigenin-7-O-glucopyranoside, luteolin-7-O-glucopyranoside, apigenin-7-O-rutinoside and acacetin-7-O-rutinoside, an unusual flavondiglucoside was isolated. Its structure was established by UV, 1HNMR and 13C NMR spectroscopic methods including 2D-NMR techniques and ESI-MS as luteolin-7,4′-O-β-diglucoside. This substance is reported for the first time in the genus Achillea. Chemotaxonomic aspects are discussed briefly

scholarly journals Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry

2016 ◽  
Vol 120 ◽  
pp. 68-71 ◽  
Author(s):  
Gerald Larrouy-Maumus ◽  
Abigail Clements ◽  
Alain Filloux ◽  
Ronan R. McCarthy ◽  
Serge Mostowy
Keyword(s):  
Mass Spectrometry ◽  
Lipid A ◽  
Direct Detection ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Fusaricidins, Polymyxins and Volatiles Produced by Paenibacillus polymyxa Strains DSM 32871 and M1

2021 ◽  
Author(s):  
Pascal Mülner ◽  
Elisa Schwarz ◽  
Kristin Dietel ◽  
Stefanie Herfort ◽  
Jennifer Jähne ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Characteristic Feature ◽  
Fungal Pathogens ◽  
Paenibacillus Polymyxa ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Antifungal Activities ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Volatile Organic

Paenibacilli are efficient producers of potent agents against bacterial and fungal pathogens, which are of great interest both for therapeutic applications in medicine as well as in agrobiotechnol-ogy. Lipopeptides produced by such organisms play a major role in their inactivation potential. In this work we investigated two lipopeptide complexes, the fusaricidins and the polymyxins, produced by Paenibacillus polymyxa strains DSM 32871 and M1 by MALDI-TOF mass spectrometry. The fusaricidins show potent antifungal activities and are distinguished by an unusual variabil-ity. For strain DSM 32871 we identified numerous yet unknown variants mass spectrometrically. DSM 32871 produces polymyxins of type E (colistins), while M1 forms polymyxins P. For both strains novel, but not yet completely characterized polymyxin species were detected, which pos-sibly are glycosylated. These compounds may be of interest therapeutically, because polymyxins attain increasing attention as last-resort antibiotics against multiresistant pathogenic Gram-negative bacteria. In addition, the volatilomes of DSM 32781 and M1 were investigated with a GC-MS approach using different cultivation media. Production of volatile organic com-pounds (VOCs) was strain and medium dependent. In particular, strain M1 manifested as an effi-cient VOC-producer that exhibited formation of 25 volatiles in total. A characteristic feature of Paenibacilli is the formation of volatile pyrazine derivatives.

scholarly journals In vitro antimicrobial and antioxidant activities of the essential oil of Craniotome furcata

2010 ◽  
Vol 2 (1) ◽  
pp. 57-62 ◽  
Author(s):  
R. K. Joshi
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Radical Cation ◽  
Steam Distillation ◽  
Antioxidant Activities ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Aerial Parts ◽  
Tube Dilution

The aim of present study was, to investigate the essential oil of Craniotome furcata for its antimicrobial and antioxidant activities. The essential oil was obtained by steam distillation of the flowering aerial parts of C. furcata. The oil was tested for its antimicrobial activity in vitro against four Gram-positive, four Gram-negative bacteria and three fungi, by using disk diffusion and tube dilution methods. The antioxidant potential of the essential oil was investigated by two different models DPPH free radical and ABTS free radical cation scavenging. The essential oil was effective against all the tested microorganisms, with minimum inhibitory concentrations (MICs) ranged from 4.11±1.44 to 0.30±0.10 mg/ml. The oil showed antioxidant activity in DPPH free radical and ABTS free radical cation scavenging model. The results of this study revealed that the essential oil of C. furcata possesses antimicrobial and antioxidant activities.

scholarly journals Antibacterial Compounds from Glycosmis puberula Twigs

2014 ◽  
Vol 9 (12) ◽  
pp. 1934578X1400901
Author(s):  
Cholpisut Tantapakul ◽  
Tawanun Sripisut ◽  
Wisanu Maneerat ◽  
Thunwadee Ritthiwigrom ◽  
Surat Laphookhieo
Keyword(s):  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Isolation And Identification ◽  
Gram Positive ◽  
Antibacterial Compounds ◽  
Gram Negative ◽  
Spectroscopic Analyses ◽  
Phytochemical Investigation ◽  
Reported Data

The first phytochemical investigation of Glycosmis puberula twigs led to the isolation and identification of a new quinolone alkaloid, glycosmispuberulone (1), along with ten known compounds (2–11). The structures were elucidated by spectroscopic analyses and comparison with previously reported data. Their antibacterial activities against Gram-positive and Gram-negative bacteria were also evaluated.

scholarly journals neo-Clerodane Diterpenoids from Scutellaria galericulata

2014 ◽  
Vol 9 (3) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Petko I. Bozov ◽  
Plamen N. Penchev ◽  
Josep Coll
Keyword(s):  
Acetone Extract ◽  
Aerial Parts ◽  
Nmr Data ◽  
Clerodane Diterpenoids ◽  
New Compounds ◽  
First Time

Four neo-clerodane diterpenoids, neoajugapyrin A, scutegalerins A and B and scutecolumnin C have been isolated from the acetone extract of the aerial parts of Scutellaria galericulata. Neoajugapyrin A and scutecolumnin C are reported in this species for the first time, whereas scutegalerins A and B are new compounds. NMR data of neoajugapyrin A are discussed in detail to support the proposed revised structure of ajugapyrin A.

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