scholarly journals Emerging biogenesis technologies of extracellular vesicles for tissue regenerative therapeutics

2021 ◽  
Vol 12 ◽  
pp. 204173142110190
Author(s):  
Jung-Hwan Lee ◽  
Ji-Young Yoon ◽  
Jun Hee Lee ◽  
Hae-Hyoung Lee ◽  
Jonathan C Knowles ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Chemical Treatment ◽  
Mass Production ◽  
Disease Status ◽  
Clinical Translation ◽  
Technological Advances ◽  
Tissue Healing ◽  
Bio Imaging ◽  
Key Issues ◽  
Treatment Application

Extracellular vesicles (EVs), including exosomes, carry the genetic packages of RNA, DNA, and proteins and are heavily involved in cell-cell communications and intracellular signalings. Therefore, EVs are spotlighted as therapeutic mediators for the treatment of injured and dysfunctional tissues as well as biomarkers for the detection of disease status and progress. Several key issues in EVs, including payload content and bioactivity, targeting and bio-imaging ability, and mass-production, need to be improved to enable effective therapeutics and clinical translation. For this, significant efforts have been made recently, including genetic modification, biomolecular and chemical treatment, application of physical/mechanical cues, and 3D cultures. Here we communicate those recent technological advances made mainly in the biogenesis process of EVs or at post-collection stages, which ultimately aimed to improve the therapeutic efficacy in tissue healing and disease curing and the possibility of clinical translation. This communication will help tissue engineers and biomaterial scientists design and produce EVs optimally for tissue regenerative therapeutics.

scholarly journals Technological advances towards extracellular vesicles mass production

2021 ◽  
pp. 113843
Author(s):  
Alice Grangier ◽  
Julien Branchu ◽  
Jeanne Volatron ◽  
Max Piffoux ◽  
Florence Gazeau ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Mass Production ◽  
Technological Advances

scholarly journals Rapid Formation of Stem Cell Spheroids Using Two-Dimensional MXene Particles

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 957
Author(s):  
JunHwee Jang ◽  
Eun-Jung Lee
Keyword(s):  
Stem Cell ◽  
Mass Production ◽  
Processing Time ◽  
Activity Levels ◽  
Two Dimensional ◽  
Spheroid Formation ◽  
Cell Spheroids ◽  
Tissue Healing ◽  
Rapid Formation ◽  
Cell Sources

Cell spheroids have been studied as a biomimic medicine for tissue healing using cell sources. Rapid cell spheroid production increases cell survival and activity as well as the efficiency of mass production by reducing processing time. In this study, two-dimensional MXene (Ti3C2) particles were used to form mesenchymal stem cell spheroids, and the optimal MXene concentration, spheroid-production times, and bioactivity levels of spheroid cells during this process were assessed. A MXene concentration range of 1 to 10 μg/mL induced spheroid formation within 6 h. The MXene-induced spheroids exhibited osteogenic-differentiation behavior, with the highest activity levels at a concentration of 5 μg/mL. We report a novel and effective method for the rapid formation of stem cell spheroids using MXene.

scholarly journals Implant Surgery Using Bio-compatible Guides

2018 ◽  
Vol 55 (1) ◽  
pp. 38-41
Author(s):  
Serban Talpos ◽  
Tareq Hajaj ◽  
Costin Timofte ◽  
Mircea Rivis ◽  
Felicia Streian ◽  
...  
Keyword(s):  
Primary Stability ◽  
Daily Practice ◽  
Implant Placement ◽  
Surgical Guides ◽  
Technological Advances ◽  
Tissue Healing ◽  
The Future ◽  
Oral Tissue ◽  
The One ◽  
User Friendly

Implants and biomaterials used in hard and soft oral tissue augmentation are very complex, but predictable to use nowadays, as the technological advances haven�t skipped this field of medicine. Cases that were impossible to treat with implant retained fixed prosthesis some years ago, have become the daily practice of oral surgeons and dentists around the world. The new user-friendly products, together with simplified protocols, increased the practitioners� predictability and success rate, thus the biomaterial industry took a huge leap forward. As the biomaterial industry keeps developing continuously, making better and safer products, the surgical and prosthetic protocols evolve and change as well. On this matter, the implant placement has become safer, using digital surgical guides. Guided implant placement doesn�t just allow the practitioner place the implant in the patient�s bone, but, moreover, it helps him place it in the correct, 3D, prosthetic position. And, thus, guiding the future bone augmentation and regeneration as well, accordingly. So, the implant placement has shifted from bone-orientated to prosthetic-orientated, offering at the same time a better primary stability for the implants, due to the prior planning. The present clinical study aims to analyze the outcome of the digital guided protocol. Unlike the free-handed surgery, the digital guided surgery allows dentists and oral surgeons to place implants according to the future prosthetic position of the crowns, even in conditions of alveolar ridges with bone resorption. Moreover, it makes possible the �one day implant� concept, the dental technician being able to create the provisional crown/s in advance, knowing precisely the future position of the implant placement. So, at the time of the surgery, the provisional crown is also put in place, guiding the soft and hard tissue healing and also giving the patient a greater satisfaction.

Optimization of Oxygen Delivery within Hydrogels

2021 ◽  
Author(s):  
Sophia M Mavris ◽  
Laura M Hansen
Keyword(s):  
Tissue Engineering ◽  
Oxygen Transport ◽  
Current Medical ◽  
The Body ◽  
Clinical Translation ◽  
Technological Innovations ◽  
Technological Advances ◽  
Cell Sources

Abstract The field of tissue engineering has been continuously evolving since its inception over three decades ago with numerous new advancements in biomaterials and cell sources and widening applications to most tissues in the body. Despite the substantial promise and great opportunities for the advancement of current medical therapies and procedures, the field has yet to capture wide clinical translation due to some remaining challenges, including oxygen availability within constructs, both in vitro and in vivo. While this insufficiency of nutrients, specifically oxygen, is a limitation within the current frameworks of this field, the literature shows promise in new technological advances to efficiently provide adequate delivery of nutrients to cells. This review attempts to capture the most recent advances in the field of oxygen transport in hydrogel-based tissue engineering, including a comparison of current research as it pertains to the modeling, sensing, and optimization of oxygen within hydrogel constructs as well as new technological innovations to overcome traditional diffusion-based limitations. The application of these findings can further the advancement and development of better hydrogel-based tissue engineered constructs for future clinical translation and adoption.

scholarly journals Low-Cost Microfabrication Tool Box

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 135 ◽  
Author(s):  
Charmet ◽  
Rodrigues ◽  
Yildirim ◽  
Challa ◽  
Roberts ◽  
...  
Keyword(s):  
Mass Production ◽  
Screen Printing ◽  
Low Cost ◽  
Unit Cost ◽  
Functional Materials ◽  
Technological Advances ◽  
Wide Range ◽  
In Vitro Diagnostic ◽  
Key Enabling Technologies

Microsystems are key enabling technologies, with applications found in almost every industrial field, including in vitro diagnostic, energy harvesting, automotive, telecommunication, drug screening, etc. Microsystems, such as microsensors and actuators, are typically made up of components below 1000 microns in size that can be manufactured at low unit cost through mass-production. Yet, their development for commercial or educational purposes has typically been limited to specialized laboratories in upper-income countries due to the initial investment costs associated with the microfabrication equipment and processes. However, recent technological advances have enabled the development of low-cost microfabrication tools. In this paper, we describe a range of low-cost approaches and equipment (below £1000), developed or adapted and implemented in our laboratories. We describe processes including photolithography, micromilling, 3D printing, xurography and screen-printing used for the microfabrication of structural and functional materials. The processes that can be used to shape a range of materials with sub-millimetre feature sizes are demonstrated here in the context of lab-on-chips, but they can be adapted for other applications. We anticipate that this paper, which will enable researchers to build a low-cost microfabrication toolbox in a wide range of settings, will spark a new interest in microsystems.

scholarly journals Automatized follow-up and alert system for patients with chronic hypertension

2020 ◽  
Vol 26 (4) ◽  
pp. 2625-2636 ◽  
Author(s):  
Claudio Urrea ◽  
Daniel Venegas
Keyword(s):  
Blood Pressure ◽  
Chronic Diseases ◽  
Monitoring System ◽  
Disease Status ◽  
Integrated System ◽  
Chronic Hypertension ◽  
Alert System ◽  
Telecommunication Technology ◽  
Technological Advances

This article presents the development and implementation of a monitoring system for patients with chronic hypertension. Technological advances in wireless communication are increasingly used today to send and receive information through smartphones. This also applies to devices for measuring blood pressure, which can be efficiently integrated with smartphones. Telemedicine is used in a variety of health fields, and in the past 5 years, it has extended its reach to the online monitoring of patients. The objective of this study is to create an integrated system capable of conducting the follow-up, through mobile communication (smartphones), of patients with chronic diseases such as hypertension. An iHealth equipment certified by the Food and Drug Administration is used. The blood pressure values from users are uploaded via Internet and stored in an integral system for processing. The monitoring system developed not only informs users about their disease status but also sends them alerts generated during monitoring. This work uses the telecommunication technology existing through smartphones. The integrated system developed ensures the follow-up of the blood pressure of a large number of users. In addition, this system can be further applied to diseases such as diabetes and metabolic syndrome. The system developed was easy to use and efficient to monitor patients with chronic diseases such as high blood pressure.

scholarly journals Extracellular Vesicles As Nanomedicine: Hopes And Hurdles In Clinical Translation

2019 ◽  
Vol Volume 14 ◽  
pp. 8847-8859 ◽  
Author(s):  
Thierry Burnouf ◽  
Vibhuti Agrahari ◽  
Vivek Agrahari
Keyword(s):  
Extracellular Vesicles ◽  
Clinical Translation

scholarly journals Mesenchymal Stem Cell-Derived Extracellular Vesicles: Opportunities and Challenges for Clinical Translation

2020 ◽  
Vol 8 ◽  
Author(s):  
Marie Maumus ◽  
Pauline Rozier ◽  
Jérémy Boulestreau ◽  
Christian Jorgensen ◽  
Danièle Noël
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Extracellular Vesicles ◽  
Clinical Translation

scholarly journals An introduction to the proceedings and a synthesis of the 2010 ICES Symposium on Fishery-Dependent Information

2011 ◽  
Vol 68 (8) ◽  
pp. 1593-1597 ◽  
Author(s):  
Norman Graham ◽  
Richard Grainger ◽  
William A. Karp ◽  
David N. MacLennan ◽  
Philip MacMullen ◽  
...  
Keyword(s):  
Fishery Management ◽  
Ecosystem Approach ◽  
Marine Science ◽  
New Approaches ◽  
Technological Advances ◽  
Key Issues

Abstract Graham, N., Grainger, R., Karp, W. A., MacLennan, D. N., MacMullen, P., and Nedreaas, K. 2011. An introduction to the proceedings and a synthesis of the 2010 ICES Symposium on Fishery-Dependent Information. – ICES Journal of Marine Science, 68: 1593–1597. Current problems and issues concerning the collection and use of fishery-dependent information are reviewed and selected papers in the pages following highlight emergent findings in the field. Key issues include relationships between stakeholders, especially fishers and scientists, and how to develop these constructively, the increasing demands on the quality and range of data on fishing activities and exploitation rates needed to promote the ecosystem approach to fishery management, and technological advances that have allowed new approaches and insights.

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