scholarly journals Detecting Streptococcus suis by nanopore sequencing in endophthalmitis: A case report

2021 ◽  
Vol 19 ◽  
pp. 205873922110026
Author(s):  
Yi-Yan Wang ◽  
Qiong Huang ◽  
Yang Cheng
Keyword(s):  
Case Report ◽  
Traditional Method ◽  
Culture Method ◽  
Streptococcus Suis ◽  
Nanopore Sequencing ◽  
Pathogenic Microorganism ◽  
Sequencing Technology ◽  
Third Generation Sequencing ◽  
Infectious Endophthalmitis ◽  
Generation Sequencing

Endophthalmitis is a rare and infectious disease caused by Streptococcus suis (S suis). Traditionally, S suis is detected by the pathogenic microorganism culture method, which has low positivity and high false negativity. Nanopore sequencing (NS), which is a third-generation sequencing technology, has several advantages over the traditional method; in particular, it is cost and time effective and has a high throughput. In this report, a case of infectious endophthalmitis caused by trauma is examined. The NS results suggest that the pathogen in question is a mixed infection caused by S suis and Clostridium perfringens. This case report provides evidence of the fact that NS can quickly identify pathogens, which is of great significance for clinical diagnosis and treatment.

scholarly journals The study of transcriptomes of symbiotic tissue of pea using the third-generation sequencing technology Oxford Nanopore

2020 ◽  
Author(s):  
E. S. Gribchenko
Keyword(s):  
Nanopore Sequencing ◽  
Third Generation ◽  
Nitrogen Fixing ◽  
Sequencing Technology ◽  
The Third ◽  
Third Generation Sequencing ◽  
Oxford Nanopore ◽  
Mycorrhizal Roots ◽  
Gene Isoforms ◽  
Generation Sequencing

The transcriptome profiles the cv. Frisson mycorrhizal roots and inoculated nitrogen-fixing nodules were investigated using the Oxford Nanopore sequencing technology. A database of gene isoforms and their expression has been created.

scholarly journals Apollo: a sequencing-technology-independent, scalable and accurate assembly polishing algorithm

2020 ◽  
Vol 36 (12) ◽  
pp. 3669-3679 ◽  
Author(s):  
Can Firtina ◽  
Jeremie S Kim ◽  
Mohammed Alser ◽  
Damla Senol Cali ◽  
A Ercument Cicek ◽  
...  
Keyword(s):  
Genome Analysis ◽  
Supplementary Information ◽  
Third Generation ◽  
Sequencing Technology ◽  
Base Pairs ◽  
Sequencing Technologies ◽  
Third Generation Sequencing ◽  
Long Reads ◽  
Generation Sequencing ◽  
Large Genomes

Abstract Motivation Third-generation sequencing technologies can sequence long reads that contain as many as 2 million base pairs. These long reads are used to construct an assembly (i.e. the subject’s genome), which is further used in downstream genome analysis. Unfortunately, third-generation sequencing technologies have high sequencing error rates and a large proportion of base pairs in these long reads is incorrectly identified. These errors propagate to the assembly and affect the accuracy of genome analysis. Assembly polishing algorithms minimize such error propagation by polishing or fixing errors in the assembly by using information from alignments between reads and the assembly (i.e. read-to-assembly alignment information). However, current assembly polishing algorithms can only polish an assembly using reads from either a certain sequencing technology or a small assembly. Such technology-dependency and assembly-size dependency require researchers to (i) run multiple polishing algorithms and (ii) use small chunks of a large genome to use all available readsets and polish large genomes, respectively. Results We introduce Apollo, a universal assembly polishing algorithm that scales well to polish an assembly of any size (i.e. both large and small genomes) using reads from all sequencing technologies (i.e. second- and third-generation). Our goal is to provide a single algorithm that uses read sets from all available sequencing technologies to improve the accuracy of assembly polishing and that can polish large genomes. Apollo (i) models an assembly as a profile hidden Markov model (pHMM), (ii) uses read-to-assembly alignment to train the pHMM with the Forward–Backward algorithm and (iii) decodes the trained model with the Viterbi algorithm to produce a polished assembly. Our experiments with real readsets demonstrate that Apollo is the only algorithm that (i) uses reads from any sequencing technology within a single run and (ii) scales well to polish large assemblies without splitting the assembly into multiple parts. Availability and implementation Source code is available at https://github.com/CMU-SAFARI/Apollo. Supplementary information Supplementary data are available at Bioinformatics online.

scholarly journals Nanopore base-calling from a perspective of instance segmentation

2019 ◽  
Author(s):  
Yao-zhong Zhang ◽  
Arda Akdemir ◽  
Georg Tremmel ◽  
Seiya Imoto ◽  
Satoru Miyano ◽  
...  
Keyword(s):  
Supplementary Information ◽  
Sequencing Technology ◽  
One Dimensional ◽  
Sequential Dependencies ◽  
Base Calling ◽  
Third Generation Sequencing ◽  
Pass Through ◽  
Segmentation Task ◽  
Generation Sequencing ◽  
Instance Segmentation

AbstractBackgroundNanopore sequencing is a rapidly developing third-generation sequencing technology, which can generate long nucleotide reads of molecules within a portable device in real time. Through detecting the change of ion currency signals during a DNA/RNA fragment’s pass through a nanopore, genotypes are determined. Currently, the accuracy of nanopore base-calling has a higher error rate than short-read base-calling. Through utilizing deep neural networks, the-state-of-the art nanopore base-callers achieve base-calling accuracy in a range from 85% to 95%.ResultIn this work, we proposed a novel base-calling approach from a perspective of instance segmentation. Different from the previous sequence labeling approaches, we formulated the base-calling problem as a multi-label segmentation task. Meanwhile, we proposed a refined U-net model which we call UR-net that can model sequential dependencies for a one-dimensional segmentation task. The experiment results show that the proposed base-caller URnano achieves competitive results compared to recently proposed CTC-featured base-caller Chiron, on the same amount of training and test data for in-domain evaluation. Our results show that formulating the base-calling problem as a one-dimensional segmentation task is a promising approach.AvailabilityThe source code and data are available at https://github.com/yaozhong/[email protected] informationSupplementary data are available at attachment online.

scholarly journals Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences

2021 ◽  
Vol 118 (8) ◽  
pp. e2007192118
Author(s):  
Wan-Chen Li ◽  
Chia-Yi Lee ◽  
Wei-Hsuan Lan ◽  
Tai-Ting Woo ◽  
Hou-Cheng Liu ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Single Molecule ◽  
Amino Acid Residues ◽  
Sequencing Technology ◽  
Structural Variations ◽  
Genome Sequences ◽  
Third Generation Sequencing ◽  
L1 And L2 ◽  
Generation Sequencing ◽  
Better Than

Most eukaryotes possess two RecA-like recombinases (ubiquitous Rad51 and meiosis-specific Dmc1) to promote interhomolog recombination during meiosis. However, some eukaryotes have lost Dmc1. Given that mammalian and yeast Saccharomyces cerevisiae (Sc) Dmc1 have been shown to stabilize recombination intermediates containing mismatches better than Rad51, we used the Pezizomycotina filamentous fungus Trichoderma reesei to address if and how Rad51-only eukaryotes conduct interhomolog recombination in zygotes with high sequence heterogeneity. We applied multidisciplinary approaches (next- and third-generation sequencing technology, genetics, cytology, bioinformatics, biochemistry, and single-molecule biophysics) to show that T. reesei Rad51 (TrRad51) is indispensable for interhomolog recombination during meiosis and, like ScDmc1, TrRad51 possesses better mismatch tolerance than ScRad51 during homologous recombination. Our results also indicate that the ancestral TrRad51 evolved to acquire ScDmc1-like properties by creating multiple structural variations, including via amino acid residues in the L1 and L2 DNA-binding loops.

scholarly journals Versatile Quality Control Methods for Nanopore Sequencing

2019 ◽  
Vol 15 ◽  
pp. 117693431986306
Author(s):  
Davide Bolognini ◽  
Roberto Semeraro ◽  
Alberto Magi
Keyword(s):  
Quality Control ◽  
Control Method ◽  
Nanopore Sequencing ◽  
Control Methods ◽  
Sequencing Data ◽  
Third Generation Sequencing ◽  
Quality Control Method ◽  
Sequencing Platforms ◽  
Generation Sequencing

Third-generation sequencing using nanopores as biosensors has recently emerged as a strategy capable to overcome next-generation sequencing drawbacks and pitfalls. Assessing the quality of the data produced by nanopore sequencing platforms is essential to decide how useful these may be in making biological discoveries. Here, we briefly contextualized NanoR, a quality control method for nanopore sequencing data we developed, in the scenario of preexistent similar tools. We also illustrated 2 quality control pipelines, readily applicable to nanopore sequencing data, respectively, based on NanoR and PyPore, a second quality control method published by our group.

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