Molecular Mechanism Underlying the Antidiabetic Effects of a Siddha Polyherbal Preparation in the Liver of Type 2 Diabetic Adult Male Rats

2012 ◽  
Vol 18 (1) ◽  
pp. 29-42 ◽  
Author(s):  
Venkataraman Balaji ◽  
Jayaraman Selvaraj ◽  
Sampath Sathish ◽  
Chinnaiyan Mayilvanan ◽  
Karundevi Balasubramanian
Keyword(s):  
Insulin Receptor ◽  
Molecular Mechanism ◽  
Diabetic Rats ◽  
Male Rats ◽  
Tinospora Cordifolia ◽  
Syzygium Aromaticum ◽  
Mrna Levels ◽  
Type 2 Diabetic ◽  
Antidiabetic Effects

A siddha polyherbal preparation consisting of 5 medicinal plants, namely, Asparagus racemosus, Emblica officinalis, Salacia oblonga, Syzygium aromaticum, and Tinospora cordifolia, in equal ratio, was formulated to examine the molecular mechanism by which it exhibits antidiabetic effects in the liver of high-fat and fructose-induced type 2 diabetic rats. The polyherbal preparation treated type 2 diabetic rats showed an increase in insulin receptor, Akt, and glucose transporter2 mRNA levels compared with diabetic rats. Insulin receptor, insulin receptor substrate-2, Akt, phosphorylated Akt substrate of 160kDaThreonine642, α-Actinin-4, β-arrestin-2, and glucose transporter2 proteins were also markedly decreased in diabetic rats, whereas the polyherbal preparation treatment significantly improved the expression of these proteins more than that of metformin-treated diabetic rats. The expression pattern of insulin signaling molecules analyzed in the present study signifies the therapeutic efficacy of the siddha polyherbal preparation.

Lactobacillus casei LC89 exert antidiabetic effects through regulating hepatic glucagon response and gut microbiota in type 2 diabetic mice

2021 ◽  
Author(s):  
Yongli Zhang ◽  
Tao Wu ◽  
Wen Li ◽  
Yunjiao Zhao ◽  
Hairong Long ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Molecular Mechanism ◽  
Lactobacillus Casei ◽  
Diabetic Mice ◽  
Antihyperglycemic Activity ◽  
Underlying Mechanisms ◽  
Type 2 Diabetic ◽  
Antidiabetic Effects

Previous study suggests Lactobacillus casei exhibit antihyperglycemic activity, however, the molecular mechanism has rarely been elucidated. Here, the anti-diabetic effects and underlying mechanisms of Lactobacillus casei LC89 were investigated in...

Synergistic effect of quercetin and 6-gingerol treatment in streptozotocin induced type 2 diabetic rats and poloxamer P-407 induced hyperlipidemia

RSC Advances ◽  
2016 ◽  
Vol 6 (15) ◽  
pp. 12235-12242 ◽  
Author(s):  
Yi Shao ◽  
Yao Yu ◽  
Cheng Li ◽  
Jing Yu ◽  
Rongrong Zong ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Diabetic Rats ◽  
Type 2 Diabetic ◽  
Antidiabetic Effects

Ginger and quercetin have been reported to have significant antidiabetic effects.

Antidiabetic effects ofEmbelia ribesextract in high fat diet and low dose streptozotocin-induced type 2 diabetic rats

2013 ◽  
Vol 7 (3-4) ◽  
pp. 186-196 ◽  
Author(s):  
Uma Bhandari ◽  
Hemantkumar Somabhai Chaudhari ◽  
Geetika Khanna ◽  
Abul Kalam Najmi
Keyword(s):  
High Fat Diet ◽  
Low Dose ◽  
Diabetic Rats ◽  
High Fat ◽  
Type 2 Diabetic ◽  
Antidiabetic Effects

Antidiabetic Effects of Total Flavonoids from Litsea Coreana leve on Fat-Fed, Streptozotocin-Induced Type 2 Diabetic Rats

2010 ◽  
Vol 38 (04) ◽  
pp. 713-725 ◽  
Author(s):  
Yun-Xia Lu ◽  
Qiu Zhang ◽  
Jun Li ◽  
Yu-Xiu Sun ◽  
Ling-Yun Wang ◽  
...  
Keyword(s):  
Body Weight ◽  
Diabetic Rats ◽  
Control Group ◽  
Chow Diet ◽  
Total Flavonoids ◽  
Alcohol Extract ◽  
Oxidation Stress ◽  
Type 2 Diabetic ◽  
Antidiabetic Effects

This study was initiated to determine the possible antidiabetic effects of total flavonoids of Litsea Coreana leve (TFLC), an alcohol extract from the dried leaves of Litsea Coreana leve, on type 2 diabetic rats. Male Sprague-Dawley rats ( n = 40, 160–180 g) were divided into two groups and fed with normal chow diet (Normal Control group) or high-fat diet (HFD) for a period of 4 weeks. After 4 weeks of dietary manipulation, the HFD-fed rats were injected with 30 mg/kg streptozocin (STZ) to induce diabetes 72 hours after STZ injection. These diabetic rats were randomly divided into 3 groups ( n = 10): Diabetic Control group, Diabetic + TFLC group and Diabetic + PIO group. Diabetic + TFLC group and Diabetic + PIO group were orally administered with 400 mg/kg TFLC or 10 mg/kg pioglitazone (all suspended in 0.5% CMC-Na) respectively for 6 weeks. All rats were examined for body weight, serum and hepatic biochemical indices, content of malondialdehyde (MDA), activities of superoxide dismutase (SOD) and pathological changes in liver and pancreas, as well as protein tyrosine phosphatase 1B (PTP1B) expression in liver. The diabetic rats became obese, insulin resistant, hyperglycemic and hyperlipidemic. Treatment with TFLC showed a significant increase in insulin sensitivity, serum HDL-C level and SOD activities, meanwhile marked decrease in body weight, serum FFA, TC, TG, LDL-C, CRP, MDA content. TFLC also attenuated pathologic alterations in liver and pancreatic islet. Furthermore, TFLC was found to decrease the expression of PTP1B in diabetic rat liver. These results suggested that TFLC could ameliorate hyperglycemia, hyperlipoidemia, inflammation and oxidation stress, as well as insulin resistance of type 2 diabetic rats.

Zhenqing Recipe Improves Glucose Metabolism and Insulin Sensitivity by Repressing Hepatic FOXO1 in Type 2 Diabetic Rats

2012 ◽  
Vol 40 (04) ◽  
pp. 721-733 ◽  
Author(s):  
Wenfan Huang ◽  
Jie Yu ◽  
Xuming Jia ◽  
Liang Xiong ◽  
Ningxu Li ◽  
...  
Keyword(s):  
Insulin Sensitivity ◽  
Glucose Metabolism ◽  
Mrna Expression ◽  
Diabetic Rats ◽  
Control Group ◽  
Oral Glucose ◽  
Sensitivity Index ◽  
Mrna Levels ◽  
Type 2 Diabetic

Forkhead box O1 (FOXO1) plays an important role in glucose metabolism at the gene transcription level. Increased FOXO1 activity results in hyperglycemia by promoting the expression of gluconeogenic enzymes such as phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase), and inhibiting glucokinase (GK). This study evaluates the effect of Zhenqing Recipe (ZQR), a Chinese herbal medicine, on hyperglycemia and its molecular mechanisms. Type 2 diabetic rats, developed by high-fat diet combined with low-dose STZ injections, were randomly divided into untreated diabetic, ZQR and metformin group. Normal rats served as control. After an eight-week treatment, fasting blood glucose was significantly decreased and insulin sensitivity index was obviously increased in the ZQR group. ZQR also improved the oral glucose tolerance. Compared with the control group, the mRNA levels of PEPCK and G6Pase were significantly elevated, while GK mRNA expression was decreased in the liver of untreated diabetic rats. ZQR significantly reduced the mRNA levels of PEPCK and G6Pase, and increased GK mRNA expression. The hepatic mRNA and protein expression of FOXO1 in the untreated diabetic group was markedly increased compared to controls. The administration of ZQR significantly decreased the mRNA and protein levels of hepatic FOXO1. The data suggest that ZQR improves glucose metabolism and insulin sensitivity, which is accompanied with regulating mRNA expression of GK and gluconeogenic genes. This anti-diabetic effect of ZQR is due to its ability to repress hepatic FOXO1 at the mRNA and protein level.

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