Numerical Modeling of Temperature-Dependent Cell Membrane Permeability to Water Based on a Microfluidic System with Dynamic Temperature Control

2021 ◽  
pp. 247263032110151
Author(s):  
Tianhang Yang ◽  
Ji Peng ◽  
Cifeng Fang ◽  
Songjing Li ◽  
Dayong Gao
Keyword(s):  
Cell Membrane ◽  
Constant Temperature ◽  
Temperature Control ◽  
Microfluidic System ◽  
Cell Membrane Permeability ◽  
Transfer Model ◽  
Temperature Dependent ◽  
Dynamic Temperature ◽  
Dependent Cell ◽  
On Chip

In order to describe temperature-dependent cell osmotic behaviors in a more reliable method, a novel mathematical mass transfer model coupled with dynamic temperature change has been established based on the combination of a time domain to temperature domain transformation equation and a constant temperature mass transfer model. This novel model is numerically simulated under multiple temperature changing rates and extracellular osmolarities. A microfluidic system that can achieve single-cell osmotic behavior observation and provide dynamic and swift on-chip temperature control was built and tested in this paper. Utilizing the temperature control system, the on-chip heating processes are recorded and then described as polynomial time–temperature relationships. These dynamic temperature changing profiles were performed by obtaining cell membrane properties by parameter fitting only one set of testing experimental data to the mathematical model with a constant temperature changing rate. The numerical modeling results show that predicting the osmotic cell volume change using selected dynamic temperature profiles is more suitable for studies concerning cell membrane permeability determination and cryopreservation process than tests using constant temperature changing rates.

Determination of the temperature-dependent cell membrane permeabilities using microfluidics with integrated flow and temperature control

Lab on a Chip ◽  
2017 ◽  
Vol 17 (5) ◽  
pp. 951-960 ◽  
Author(s):  
Cifeng Fang ◽  
Fujun Ji ◽  
Zhiquan Shu ◽  
Dayong Gao
Keyword(s):  
Cell Membrane ◽  
Temperature Control ◽  
Temperature Dependent ◽  
Microfluidic Platform ◽  
Instantaneous Flow ◽  
Dependent Cell ◽  
Membrane Permeabilities

We developed an integrated microfluidic platform for instantaneous flow and localized temperature control.

Fluorescence imaging of cholesterol and temperature dependent cell membrane dynamics

2007 ◽  
Author(s):  
Petra Weber ◽  
Michael Wagner ◽  
Wolfgang S. L. Strauss ◽  
Herbert Schneckenburger
Keyword(s):  
Cell Membrane ◽  
Fluorescence Imaging ◽  
Membrane Dynamics ◽  
Temperature Dependent ◽  
Dependent Cell

scholarly journals Determination of the Membrane Transport Properties of Jurkat Cells with a Microfluidic Device

Micromachines ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 832
Author(s):  
Tianhang Yang ◽  
Ji Peng ◽  
Zhiquan Shu ◽  
Praveen K. Sekar ◽  
Songjing Li ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Transport Properties ◽  
Membrane Transport ◽  
Jurkat Cell ◽  
Adoptive Cell Therapy ◽  
Microfluidic System ◽  
Jurkat Cells ◽  
Cell Membrane Permeability ◽  
Specific Protocol

The Jurkat cell is an immortalized line of human acute lymphocyte leukemia cells that is widely used in the study of adoptive cell therapy, a novel treatment of several advanced forms of cancer. The ability to transport water and solutes across the cell membrane under different temperatures is an important factor for deciding the specific protocol for cryopreservation of the Jurkat cell. In this study we propose a comprehensive process for determination of membrane transport properties of Jurkat cell. using a novel microfluidic controlled single cell-trapping system. The osmotic behavior of an individual Jurkat cell to water and dimethyl sulfoxide (DMSO), a commonly used cryoprotective agent (CPA), under constant temperature, was recorded under a microscope utilizing the modified microfluidic system. The images of the Jurkat cell under osmotic change were processed to obtain a relationship between cell volume change and time. The experimental results were fitted using a two-parameter transport numeric model to calculate the Jurkat cell membrane permeability to water and DMSO at room temperature (22 °C). This model and the calculated parameters can help scientists optimize the cryopreservation protocol for any cell type with optimal cryoprotective agents and cooling rate for future experiments.

On-chip rapid drug screening of leukemia cells by acoustic streaming

Lab on a Chip ◽  
2021 ◽  
Vol 21 (20) ◽  
pp. 4005-4015
Author(s):  
Shu-Kun Zhao ◽  
Xue-Jia Hu ◽  
Jiao-Meng Zhu ◽  
Zi-Yi Luo ◽  
Li Liang ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Drug Screening ◽  
Drug Concentration ◽  
Drug Absorption ◽  
Concentration Gradient ◽  
Acoustic Streaming ◽  
Cell Membrane Permeability ◽  
Leukemia Cells ◽  
Gradient Field ◽  
On Chip

An acoustic streaming based rapid single-cell drug screening microfluidic device is developed. It is achieved by acoustic streaming non-invasively changing cell membrane permeability to accelerate drug absorption in drug concentration gradient field.

Sarcolemmal permeability changes during early myocardial anoxia

1978 ◽  
Vol 36 (2) ◽  
pp. 642-643
Author(s):  
M. Ashraf ◽  
L. Landa ◽  
L. Nimmo ◽  
C. M. Bloor
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Cell Membrane Permeability ◽  
Enzyme Release ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Sarcolemmal Permeability ◽  
Membrane Changes

Following coronary artery occlusion, the myocardial cells lose intracellular enzymes that appear in the serum 3 hrs later. By this time the cells in the ischemic zone have already undergone irreversible changes, and the cell membrane permeability is variably altered in the ischemic cells. At certain stages or intervals the cell membrane changes, allowing release of cytoplasmic enzymes. To correlate the changes in cell membrane permeability with the enzyme release, we used colloidal lanthanum (La+++) as a histological permeability marker in the isolated perfused hearts. The hearts removed from sprague-Dawley rats were perfused with standard Krebs-Henseleit medium gassed with 95% O2 + 5% CO2. The hypoxic medium contained mannitol instead of dextrose and was bubbled with 95% N2 + 5% CO2. The final osmolarity of the medium was 295 M osmol, pH 7. 4.

scholarly journals Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor †

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1178 ◽  
Author(s):  
Jorge Prada ◽  
Christina Cordes ◽  
Carsten Harms ◽  
Walter Lang
Keyword(s):  
Microfluidic Device ◽  
Heating Temperature ◽  
Low Cost ◽  
Reaction Chamber ◽  
Microfluidic System ◽  
Heating Process ◽  
Design And Manufacturing ◽  
On Chip ◽  
Working Parameters ◽  
Auto Fluorescence

This contribution outlines the design and manufacturing of a microfluidic device implemented as a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval was carried on after bacteria heat-lysis by an on-chip micro-heater, whose function was characterized at different working parameters. Carbon resistive temperature sensors were tested, characterized and printed on the biochip sealing film to monitor the heating process. Off-chip and on-chip processed RNA were hybridized with capture probes on the reaction chamber surface and identification was achieved by detection of fluorescence tags. The application of the mentioned techniques and materials proved to allow the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the integration potential of fully thermoplastic devices in biosensor systems.

Closed-loop feedback control of microfluidic cell manipulation via deep-learning integrated sensor networks

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Ningquan Wang ◽  
Ruxiu Liu ◽  
Norh Asmare ◽  
Chia-Heng Chu ◽  
Ozgun Civelekoglu ◽  
...  
Keyword(s):  
Deep Learning ◽  
Sensor Networks ◽  
Feedback Control ◽  
Closed Loop ◽  
Microfluidic System ◽  
Cell Manipulation ◽  
Integrated Sensor ◽  
Loop Feedback ◽  
Closed Loop Feedback ◽  
On Chip

An adaptive microfluidic system changing its operational state in real-time based on cell measurements through an on-chip electrical sensor network.

scholarly journals Induction of macrophage pyroptosis-related factors by pathogenic E. coli high pathogenicity island (HPI) in Yunnan Saba pigs

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Chunlan Shan ◽  
Shushu Miao ◽  
Chaoying Liu ◽  
Bo Zhang ◽  
Weiwei Zhao ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Inflammatory Diseases ◽  
Pathogenicity Island ◽  
Cell Membrane Permeability ◽  
Inflammatory Factor ◽  
Related Factors ◽  
Membrane Pore ◽  
E Coli ◽  
Caspase 1 ◽  
High Pathogenicity

Abstract Background Pyroptosis plays a pivotal role in the pathogenesis of many inflammatory diseases. The molecular mechanism by which pyroptosis is induced in macrophages following infection with pathogenic E. coli high pathogenicity island (HPI) will be evaluated in our study. Results After infection with the HPI+/HPI− strains and LPS, decreased macrophage cell membrane permeability and integrity were demonstrated with propidium iodide (PI) staining and the lactate dehydrogenase (LDH) assay. HPI+/HPI−-infection was accompanied by upregulated expression levels of NLRP3, ASC, caspase-1, IL-1β, IL-18 and GSDMD, with significantly higher levels detected in the HPI+ group compared to those in the HPI− group (P < 0.01 or P < 0.05). HPI+ strain is more pathogenic than HPI− strain. Conclusion Our findings indicate that pathogenic E. coli HPI infection of Saba pigs causes pyroptosis of macrophages characterized by upregulated expression of pyroptosis key factors in the NLRP3/ASC/caspase-1 signaling pathway, direct cell membrane pore formation, and secretion of the inflammatory factor IL-1β and IL-18 downstream of NLRP3 and caspase-1 activation to enhance the inflammatory response.

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