scholarly journals Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor †

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1178 ◽  
Author(s):  
Jorge Prada ◽  
Christina Cordes ◽  
Carsten Harms ◽  
Walter Lang
Keyword(s):  
Microfluidic Device ◽  
Heating Temperature ◽  
Low Cost ◽  
Reaction Chamber ◽  
Microfluidic System ◽  
Heating Process ◽  
Design And Manufacturing ◽  
On Chip ◽  
Working Parameters ◽  
Auto Fluorescence

This contribution outlines the design and manufacturing of a microfluidic device implemented as a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval was carried on after bacteria heat-lysis by an on-chip micro-heater, whose function was characterized at different working parameters. Carbon resistive temperature sensors were tested, characterized and printed on the biochip sealing film to monitor the heating process. Off-chip and on-chip processed RNA were hybridized with capture probes on the reaction chamber surface and identification was achieved by detection of fluorescence tags. The application of the mentioned techniques and materials proved to allow the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the integration potential of fully thermoplastic devices in biosensor systems.

scholarly journals Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Biosensor

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 810
Author(s):  
Jorge Prada ◽  
Christina Cordes ◽  
Carsten Harms ◽  
Walter Lang
Keyword(s):  
Heating Temperature ◽  
Low Cost ◽  
Reaction Chamber ◽  
Microfluidic System ◽  
Heating Process ◽  
Design And Manufacturing ◽  
Microfluidic Biosensor ◽  
On Chip ◽  
Auto Fluorescence ◽  
Reaction Processes

This contribution outlines the design and manufacturing of a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval is carried on after bacteria heat-lysis by an on-chip micro-heater. Two additional carbon resistive temperature sensors printed on the biochip sealing film monitor the heating process. RNA is hybridized with capture probes on the reaction chamber surface and identification is achieved by detection of fluorescence tags. The application of the mentioned techniques and materials facilitates the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the potential of fully thermoplastic devices as biosensors.

Low-cost polymer microfluidic device for on-chip extraction of bacterial DNA

2011 ◽  
Vol 155 (1) ◽  
pp. 422-429 ◽  
Author(s):  
Kyu-Youn Hwang ◽  
Joon-Ho Kim ◽  
Kahp-Yang Suh ◽  
Jong Soo Ko ◽  
Nam Huh
Keyword(s):  
Microfluidic Device ◽  
Low Cost ◽  
Bacterial Dna ◽  
On Chip

On–Chip Droplets Sensing using Capacitive Technique

2013 ◽  
Vol 61 (2) ◽  
Author(s):  
Mohamad Faizal Abdullah ◽  
P. L. Leow ◽  
M. A. Abd Razak ◽  
F. K. Che Harun
Keyword(s):  
Low Cost ◽  
Microfluidic Channel ◽  
High Sensitivity ◽  
Microfluidic Devices ◽  
Capacitive Sensor ◽  
Sample Volume ◽  
Microfluidic System ◽  
Coplanar Electrodes ◽  
On Chip ◽  
Significant Attention

Significant attention has been given on the development of droplets–based microfluidic system because of its potential and apparent advantages. Beside the advantages of reducing the sample volume, it’s also offer less time consuming for the analysis. Optical and fluorescence among the famous method that was used in detection of droplets but they are normally bulky, expensive and not easily accessed. This paper proposed a simple, low cost and high sensitivity for droplets sensing in microfluidic devices by using capacitive sensor. Coplanar electrodes are used to form a capacitance through the microfluidic channel. The design of eight pair of electrodes was used to detect the presence of a droplet. Changes in capacitance due to the presence of a droplet in the sensing area is detected and used to trigger the microscope to capture the image of detected droplets in microchannel. The measurement of droplets detected and counting are displayed through a LABVIEW interface in the real time.

scholarly journals Regenerable Bead-Based Microfluidic Device with integrated THIN-Film Photodiodes for Real Time Monitoring of DNA Detection

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 953
Author(s):  
Catarina R. F. Caneira ◽  
Denis R. Santos ◽  
Virginia Chu ◽  
João P. Conde
Keyword(s):  
Thin Film ◽  
Real Time ◽  
Microfluidic Device ◽  
Low Cost ◽  
High Sensitivity ◽  
Significant Loss ◽  
Real Time Monitoring ◽  
Target Dna ◽  
On Chip ◽  
Biosensing System

Nanoporous microbead-based microfluidic systems for biosensing applications allow enhanced sensitivities, while being low cost and amenable for miniaturization. The regeneration of the microfluidic biosensing system results in a further decrease in costs while the integration of on-chip signal transduction enhances portability. Here, we present a regenerable bead-based microfluidic device, with integrated thin-film photodiodes, for real-time monitoring of molecular recognition between a target DNA and complementary DNA (cDNA). High-sensitivity assay cycles could be performed without significant loss of probe DNA density and activity, demonstrating the potential for reusability, portability and reproducibility of the system.

scholarly journals Microoxygraph Device for Biosensoristic Applications

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
A. Aloisi ◽  
E. Tarentini ◽  
A. Ferramosca ◽  
V. Zara ◽  
R. Rinaldi
Keyword(s):  
Response Time ◽  
Low Cost ◽  
Reference Electrode ◽  
Reaction Chamber ◽  
Counter Electrodes ◽  
Permeable Membrane ◽  
Lab On Chip ◽  
Lift Off ◽  
On Chip

Oxygen consumption rate (OCR) is a significant parameter helpful to determinein vitrorespiratory efficiency of living cells. Oxygen is an excellent oxidant and its electrocatalytic reduction on a noble metal allows accurately detecting it. By means of microfabrication technologies, handy, low-cost, and disposable chip can be attained, minimizing working volumes and improving sensitivity and response time. In this respect, here is presented a microoxygraph device (MOD), based on Clark’s electrode principle, displaying many advantageous features in comparison to other systems. This lab-on-chip platform is composed of a three-microelectrode detector equipped with a microgrooved electrochemical cell, sealed with a polymeric reaction chamber. Au working/counter electrodes and Ag/AgCl reference electrode were fabricated on a glass slide. A microchannel was realized by photoresist lift-off technique and a polydimethylsiloxane (PDMS) nanoporous film was integrated as oxygen permeable membrane (OPM) between the probe and the microreaction chamber. Electrochemical measurements showed good reproducibility and average response time, assessed by periodic injection and suction of a reducing agent. OCR measurements on 3T3 cells, subjected, in real time, to chemical stress on the respiratory chain, were able to show that this chip allows performing consistent metabolic analysis.

A piezo-ring-on-chip microfluidic device for simple and low-cost mass spectrometry interfacing

The Analyst ◽  
2018 ◽  
Vol 143 (4) ◽  
pp. 981-988 ◽  
Author(s):  
Chia-Wen Tsao ◽  
I-Chao Lei ◽  
Pi-Yu Chen ◽  
Yu-Liang Yang
Keyword(s):  
Mass Spectrometry ◽  
Microfluidic Device ◽  
Low Cost ◽  
Ms Analysis ◽  
On Chip

Mass spectrometry (MS) interfacing technology provides the means for incorporating microfluidic processing with post MS analysis.

scholarly journals Low Cost Microfluidic Device for Assaying Blood Glucose

2019 ◽  
Vol 16 (2) ◽  
pp. 279-287
Author(s):  
Azmi Naqvi ◽  
Dinesh C. Sharma ◽  
Pradip Nahar
Keyword(s):  
Silica Gel ◽  
Microfluidic Device ◽  
Gravitational Force ◽  
Low Cost ◽  
Microfluidic System ◽  
Diagnostic Purpose ◽  
Pure Silica ◽  
External Power ◽  
Gravitational Pull

Herein, gravitational force based low cost colorimetric microfluidic device is developed for diagnostic purpose. Microfluidic system is developed by using discarded pen refills. Refill is filled with three layers of polymer. Bottom of the refill is filled with the polymer polyvinyl chloride (PVC). Second layer from the bottom i.e the layer above PVC layer is filled with silica gel immobilized with horse radish peroxidase (HRP), glucose oxidase (GOD) and o- dianisidine (dye). Whereas, third and the top most layer is filled with untreated silica gel. One drop of blood is poured at the inlet of microfluidic device. Without applying any external power, blood moves through the silica packed region by gravitational pull and capillary action of silica gel. Serum separation started within 30 seconds and subsequently within 2 min., serum successfully separates from blood by pure silica gel. The separated serum then comes in contact with the silica gel immobilized with enzymes and dye. The colour of the silica beads immobilized with enzymes and dye changes from white to orange when comes in contact with glucose in serum. Determination of the glucose in the blood is carried out on a desktop scanner. The developed microfluidic device do not require (i) pump or device to propel the fluid (ii) any type of special mesh or sieve to separate the serum from the blood. Microfluidic device developed is cheap and suitable for low cost setting areas.

Study of Liquid Metal Based Thermal Micro-System for On-Chip Cell Culture Purpose

2013 ◽  
Author(s):  
Meng Gao ◽  
Lin Gui
Keyword(s):  
Cell Culture ◽  
Temperature Distribution ◽  
Liquid Metal ◽  
Temperature Sensor ◽  
Low Cost ◽  
Protein Crystallization ◽  
Heating Process ◽  
Temperature Liquid ◽  
On Chip ◽  
Accurate Temperature Control

Room temperature liquid metal has been widely used in many MEMS applications, such as integrated heaters, sensors, electrodes and stretchable wires. Injecting the liquid metal into microchannels provides a simple, rapid and low-cost way to fabricate micro heaters and sensors. The liquid-metal-filled microstructures can be designed in any shape and easily integrated into micro devices. In this study, a liquid-metal based thermal micro-system was proposed for on-chip cell culture purpose. The thermal micro-system consisted of two same microchannels filled with the liquid metal as electrical heaters. At the same time, the heater also worked as a resistance temperature sensor to control the heating process. The temperature sensor was calibrated from 20 °C to 70 °C to give an accurate temperature control for the microsystem. To justify whether this micro-system is capable of providing a uniform temperature distribution, Rhodamine B was filled into the micro cell culture chamber of interest to monitor the temperature distribution. Thermal analysis was numerically carried out to reveal the temperature field of the chip. This thermal micro-system has great potential use in many microfluidic applications, such as on-chip PCR, temperature gradient focusing, protein crystallization or chemical synthesis.

Disposable Smart Plastic Biochips For Clinical Diagnostics

2002 ◽  
Vol 729 ◽  
Author(s):  
Chong H. Ahn ◽  
Jin-Woo Choi ◽  
Sanghyo Kim ◽  
Young-Soo Sohn ◽  
Aniruddha Puntambekar ◽  
...  
Keyword(s):  
Passive Control ◽  
Point Of Care ◽  
Low Cost ◽  
Microfluidic System ◽  
Clinical Diagnostics ◽  
Plastic Substrate ◽  
Driving Mechanisms ◽  
Care Health ◽  
Diagnostic Applications ◽  
On Chip

AbstractThis paper presents an overview of the development of novel disposable smart plastic fluidic biochips for clinical diagnostic applications. The biochip is manufactured using a low-cost, rapid turn around injection molding/embossing process on a plastic substrate. The plastic fluidic biochip uses a novel sPROMs (structurally programmable microfluidic system) approach to achieve passive control of fluidic sequencing [1-2]. The plastic biochip also uses an on-chip pressurized air source for fluidic movement thus eliminating the need for active driving mechanisms and allowing for a truly disposable approach. Furthermore, electrochemical biosensors are also integrated on-chip to analyze various metabolically significant parameters such as PO2(partial pressure of oxygen), Glucose, Lactate,and pH. The fluidic biochip is being developed for point-of-care health monitoring applications where parameters such as small size, simplicity of operation, disposability, reduced cross-contamination are vital. The issues mentioned above are successfully addressed using the approach of this work and are discussed in this paper.

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