scholarly journals Immunohistochemical demonstration of Fc receptors of human lymph node cells using peroxidase-antiperoxidase (IgG).

1980 ◽  
Vol 28 (3) ◽  
pp. 271-275 ◽  
Author(s):  
G Itoh
Keyword(s):  
Lymph Node ◽  
Cell Surface ◽  
Microscopic Examination ◽  
Fc Receptors ◽  
Electron Microscopic Examination ◽  
Reaction Products ◽  
Electron Microscopic ◽  
Regional Lymph Nodes ◽  
Human Lymph Node ◽  
Lymph Node Cells

Human lymph node cells, prepared from regional lymph nodes excised from four patients with gastric cancer, were incubated with peroxidase-antiperoxidase (IgG) (PAPIgG). After being washed, they were reacted with diaminobenzidine tetrahydrochloride in the presence of H2O2. Light microscopic examination revealed that a certain proportion of lymph node cells (18.2-32.2%) were labeled on their cell surface with brown-colored reaction products and that the labeled cells were composed of small lymphocytes. Electron microscopic examination demonstrated electron-dense irregular-shaped aggregates of reaction products on the cell surface of lymphocytes. Characterization experiments confirmed that the immune complexes of PAPIgG bound specifically with Fc receptors. PAPIgG, therefore, can be used as a specific indicator for Fc receptor of human lymph node cells.

scholarly journals Immunohistochemical detection of Fc receptor. II. Electron microscopic demonstration of Fc receptor by using soluble immune complexes of ferritin-antiferritin immunoglobulin G.

1977 ◽  
Vol 25 (4) ◽  
pp. 259-265 ◽  
Author(s):  
G Itoh ◽  
I Suzuki
Keyword(s):  
Lymph Node ◽  
Cell Surface ◽  
Immunoglobulin G ◽  
Immune Complexes ◽  
Fc Receptor ◽  
Electron Microscopic ◽  
Microscopic Features ◽  
Lymph Node Cells ◽  
Soluble Immune Complexes ◽  
Ethanol Series

The mouse mesenteric lymph node cells were incubated in the soluble immune complexes of ferritin-antiferritin immunoglobulin G at 37 degrees C for 20 min. After being washed, postfixed with OsO4 and dehydrated by degraded ethanol series, the lymph node cells were observed by electron microscope. Apprroximately 15% of the cells (mainly composed of small lymphocytes) bound ferritin particles to the cell surface. The distribution pattern of the binding of ferritin particles (ferritin-antiferritin immunoglobulin G) took the form of discrete patches of irregular distribution interspaced with unlabeled portions. The electron microscopic features of ferritin particles (ferritin-antiferritin immunoglobulin G) attached to the cell surface suggest that a structure of constant conformation (Fc receptor) situated in the cell membrane takes part in the binding of ferritin-antiferritin immunoglobulin G.

scholarly journals Brief Report: Nuclear Bodies of Normal and Pathological Human Lymph Node Cells: An Electron Microscopic Study

Blood ◽  
1967 ◽  
Vol 29 (2) ◽  
pp. 269-275 ◽  
Author(s):  
ROBERT E. BROOKS ◽  
BENJAMIN V. SIEGEL
Keyword(s):  
Electron Microscope ◽  
Lymph Node ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Nuclear Bodies ◽  
Electron Microscopic ◽  
Dense Granules ◽  
Fibrillar Material ◽  
Human Lymph Node ◽  
Lymph Node Cells

Abstract Nuclear bodies in normal and pathologic human lymph node cells have been examined with the electron microscope and their structure has been illustrated and described. In normal lymph node cells, nuclear bodies are 0.3-0.5 microns in diameter, are slightly less electron dense than the nucleolus, and consist of peripheral fibrillar material with centrally located, dense granules, 200-400 Å in diameter. Morphologically abnormal nuclear bodies have been observed in a case of Hodgkin’s disease. The appearance of these atypical bodies would suggest either contact and fusion of two or more atypical bodies, or possibly the existence of single, large, irregular bodies.

A dermatosis resembling juvenile cellulitis in an adult dog

1995 ◽  
Vol 31 (3) ◽  
pp. 204-208 ◽  
Author(s):  
JG Jeffers ◽  
DD Duclos ◽  
MH Goldschmidt
Keyword(s):  
Lymph Node ◽  
Microscopic Examination ◽  
Otitis Externa ◽  
Histopathological Examination ◽  
Electron Microscopic Examination ◽  
Acute Onset ◽  
Adult Case ◽  
Electron Microscopic

A two-year-old, female Lhasa apso presented with an acute onset of fever, anorexia, lethargy, prescapular and mandibular lymphadenopathy, otitis externa, and a dermatitis involving the perioral and auricular skin. Histopathological examination of affected skin and a mandibular lymph node was diagnostic for juvenile cellulitis. Extensive hematological, serological, urine, skin, and fecal testing together with special staining, immunofluorescence, and electron microscopic examination of skin and lymph node biopsies failed to reveal an underlying etiology. After 15 weeks the condition resolved completely. This represents the first adult case of a dermatosis fitting the clinical, histological, and clinicopathological description ascribed to juvenile cellulitis.

A study of specialized cells of the tracheal gills of Paragnetina media (Plecoptera)

1973 ◽  
Vol 51 (9) ◽  
pp. 983-986 ◽  
Author(s):  
N. N. Kapoor ◽  
K. Zachariah
Keyword(s):  
Plasma Membrane ◽  
Cell Surface ◽  
Microscopic Examination ◽  
External Environment ◽  
Electron Microscopic Examination ◽  
Active Surface ◽  
Electron Microscopic ◽  
Close Relationship ◽  
Paragnetina Media ◽  
Tracheal Gills

Light and electron microscopic examination of the gills of the plecopteran nymph Paragnetina media revealed a highly tracheated epithelium with many specialized cells. These cells show several features characteristic of the osmoregulatory tissue of other animals. In the cell, numerous mitochondria are lodged in elongated folds of the plasma membrane in such a way that they are brought into a very close relationship to an area of the cell surface. It is assumed that this arrangement provides metabolically active surface for the exchange of materials, absorption, or excretion. It is most likely that the gills can absorb salt from the water, and thus compensate for the loss of salt through the urine. The distinctive cuticular plaque which forms the interface of each cell with the external environment is featured and discussed too.

scholarly journals Normal Human Lymph Node Cells: An Electron Microscopic Study

Blood ◽  
1966 ◽  
Vol 27 (5) ◽  
pp. 687-705 ◽  
Author(s):  
ROBERT E. BROOKS ◽  
BENJAMIN V. SIEGEL
Keyword(s):  
Lymph Node ◽  
Lymphoid Tissue ◽  
Plasma Cells ◽  
Cell Types ◽  
Lymph Node Cell ◽  
Electron Microscopic ◽  
Human Lymph Node ◽  
Lymph Node Cells ◽  
Normal Human ◽  
Characteristic Features

Abstract Lymph nodes, from 15 patients undergoing surgery for conditions not related to lymphoid tissue disease, have been examined with the electron microscope. The human lymph node cell types—including lymphocytic, reticular and plasma cells—have been described at low and medium electron microscopic magnifications, and the criteria for their identification are discussed. The characteristic features outlined for identification of these cell types provide a basis for comparison with pathologically altered lymph node cells.

Properties of Isolated Mitochondria of Agaricus Bisporus: Their Relationship to Dormancy and the Germination of Spores

1973 ◽  
Vol 31 ◽  
pp. 458-459
Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel
Keyword(s):  
Mitochondrial Dna ◽  
Ethidium Bromide ◽  
Microscopic Examination ◽  
Agaricus Bisporus ◽  
Osmotic Shock ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Isolated Mitochondria ◽  
Dna Strands ◽  
Cytoplasmic Ribosomes

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.

Artifact-free electron microscopic immunocytochemistry on rat brain tissue

1991 ◽  
Vol 49 ◽  
pp. 272-273
Author(s):  
Veronika Burmeister ◽  
N. Ludvig ◽  
P.C. Jobe
Keyword(s):  
Microscopic Examination ◽  
Free Electron ◽  
Electron Microscopic Examination ◽  
Ultrastructural Localization ◽  
Rabbit Serum ◽  
Electron Microscopic ◽  
Electron Microscopic Immunocytochemistry ◽  
Phosphate Buffered Saline ◽  
Rat Brain Tissue ◽  
Immune Rabbit

Electron microscopic immunocytochemistry provides an important tool to determine the ultrastructural distribution of various molecules in both normal and pathologic tissues. However, the specific immunostaining may be obscured by artifactual immunoreaction product, misleading the investigator. Previous observations show that shortening the incubation period with the primary antibody from the generally used 12-24 hours to 1 hour substantially reduces the artifactual immunostaining. We now extend this finding by the demonstration of artifact-free ultrastructural localization of the Ca2/calmodulindependent cyclic nucleotide phosphodiesterase (CaM-dependent PDE) immunoreactivity in brain.Anesthetized rats were perfused transcardially with phosphate-buffered saline followed by a fixative containing paraformaldehyde (4%) and glutaraldehyde (0.25%) in PBS. The brains were removed, and 40μm sections were cut with a vibratome. The sections were processed for immunocytochemistry as described by Ludvig et al. Both non-immune rabbit serum and specific CaM-dependent PDE antibodies were used. In both experiments incubations were at one hour and overnight. The immunostained sections were processed for electron microscopic examination.

Ultrastructural studies of Chlamydia psittaci 6BC variant strains. I. Ultrastructure of the surface layers of egg-passaged 6BC strain

1973 ◽  
Vol 19 (8) ◽  
pp. 887-894
Author(s):  
Linda Poffenroth ◽  
J. W. Costerton ◽  
Nonna Kordová ◽  
John C. Wilt
Keyword(s):  
Chick Embryo ◽  
Microscopic Examination ◽  
Electron Microscopic Examination ◽  
Chlamydia Psittaci ◽  
Gram Negative Bacteria ◽  
Surface Layers ◽  
Electron Microscopic ◽  
Gram Negative ◽  
Ultrastructural Studies ◽  
First Time

Electron microscopic examination of a semipurified Chlamydia psittaci 6BC strain attenuated in chick embryo yolk sac revealed for the first time two morphologically distinct small elementary bodies which differ both in the ultrastructure of their surface layers and in their buoyant densities in sucrose gradients. Also, the morphology of the surface layers of the larger reticulate forms in cell-free systems is described for the first time. Many points of difference between the surface envelopes and internal structure of chlamydial particles and those of Gram-negative bacteria are discussed.

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