scholarly journals Normal Human Lymph Node Cells: An Electron Microscopic Study

Blood ◽  
1966 ◽  
Vol 27 (5) ◽  
pp. 687-705 ◽  
Author(s):  
ROBERT E. BROOKS ◽  
BENJAMIN V. SIEGEL
Keyword(s):  
Lymph Node ◽  
Lymphoid Tissue ◽  
Plasma Cells ◽  
Cell Types ◽  
Lymph Node Cell ◽  
Electron Microscopic ◽  
Human Lymph Node ◽  
Lymph Node Cells ◽  
Normal Human ◽  
Characteristic Features

Abstract Lymph nodes, from 15 patients undergoing surgery for conditions not related to lymphoid tissue disease, have been examined with the electron microscope. The human lymph node cell types—including lymphocytic, reticular and plasma cells—have been described at low and medium electron microscopic magnifications, and the criteria for their identification are discussed. The characteristic features outlined for identification of these cell types provide a basis for comparison with pathologically altered lymph node cells.

scholarly journals PSVI-26 Cytological picture of the lymphoid tissue in the inguinal node with calval leptospirose

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 209-210
Author(s):  
Kirill Plemyashov ◽  
Suleyman Suleymanov ◽  
Konstantin Lobodin ◽  
Olga Pavlenko
Keyword(s):  
Lymph Node ◽  
Lymphoid Tissue ◽  
Plasma Cells ◽  
Inguinal Lymph Node ◽  
Cell Types ◽  
Reticular Cell ◽  
Study Material ◽  
Cytological Picture ◽  
Reticular Cells ◽  
Neutral Formalin

Abstract In this regard, the cytological picture of the lymphoid tissue in calves’ inguinal lymph node with spontaneous leptospirosis was studied. The study material was taken from the inguinal lymph node of the 11 calves who died of leptospirosis during the enzootic period in Azerbaijan. The study material samples were fixed in 10% neutral formalin solution, followed by pouring in paraffin, coloring azur sections with 2-eosin and counting 13 cell types (lymphoblast, prolymphocyte, lymphocyte, free reticular cell, process sinus reticular cell, endothelium, fibroblast, histiocyte, macrophage, polyblast, plasmablast, protoplasmocyte, plasmacyte) using MOV-15. It was established that the number of lymphoblasts in the inguinal lymph node with subacute leptospirosis decreased 2.2 times, the number of prolymphocytes decreased 1.4 times, the number of lymphocytes decreased 4.4 times. The number of free reticular cells from the cells of the reticuloendothelium decreased 3.7 times. However, the number of grown sinus reticular cells and the endothelium of the sinuses fluctuated within the normal range. The number of fibroblasts increased 1.7 times, histiocytes - 6.6 times, macrophages - 11.8 times, and polyblasts - 11 times (Table 1). At the same time, there was a sharp increase in the number of cells in the plasma row. Of those, the number of plasmablasts increased 8.5 times, protoplasmocytes - 30.4 times, plasma cells - 17 times. Overall, the cytological picture in the inguinal lymph node during spontaneous leptospirosis in calves was characterized by an increase in the number of plasma cells, fibroblasts, histiocytes, macrophages, polyblasts and a decrease in the number of lymphoblasts, prolymphocytes, lymphocytes and free reticular cells.

scholarly journals Immunohistochemical demonstration of Fc receptors of human lymph node cells using peroxidase-antiperoxidase (IgG).

1980 ◽  
Vol 28 (3) ◽  
pp. 271-275 ◽  
Author(s):  
G Itoh
Keyword(s):  
Lymph Node ◽  
Cell Surface ◽  
Microscopic Examination ◽  
Fc Receptors ◽  
Electron Microscopic Examination ◽  
Reaction Products ◽  
Electron Microscopic ◽  
Regional Lymph Nodes ◽  
Human Lymph Node ◽  
Lymph Node Cells

Human lymph node cells, prepared from regional lymph nodes excised from four patients with gastric cancer, were incubated with peroxidase-antiperoxidase (IgG) (PAPIgG). After being washed, they were reacted with diaminobenzidine tetrahydrochloride in the presence of H2O2. Light microscopic examination revealed that a certain proportion of lymph node cells (18.2-32.2%) were labeled on their cell surface with brown-colored reaction products and that the labeled cells were composed of small lymphocytes. Electron microscopic examination demonstrated electron-dense irregular-shaped aggregates of reaction products on the cell surface of lymphocytes. Characterization experiments confirmed that the immune complexes of PAPIgG bound specifically with Fc receptors. PAPIgG, therefore, can be used as a specific indicator for Fc receptor of human lymph node cells.

scholarly journals Brief Report: Nuclear Bodies of Normal and Pathological Human Lymph Node Cells: An Electron Microscopic Study

Blood ◽  
1967 ◽  
Vol 29 (2) ◽  
pp. 269-275 ◽  
Author(s):  
ROBERT E. BROOKS ◽  
BENJAMIN V. SIEGEL
Keyword(s):  
Electron Microscope ◽  
Lymph Node ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Nuclear Bodies ◽  
Electron Microscopic ◽  
Dense Granules ◽  
Fibrillar Material ◽  
Human Lymph Node ◽  
Lymph Node Cells

Abstract Nuclear bodies in normal and pathologic human lymph node cells have been examined with the electron microscope and their structure has been illustrated and described. In normal lymph node cells, nuclear bodies are 0.3-0.5 microns in diameter, are slightly less electron dense than the nucleolus, and consist of peripheral fibrillar material with centrally located, dense granules, 200-400 Å in diameter. Morphologically abnormal nuclear bodies have been observed in a case of Hodgkin’s disease. The appearance of these atypical bodies would suggest either contact and fusion of two or more atypical bodies, or possibly the existence of single, large, irregular bodies.

scholarly journals ELECTRON MICROSCOPIC OBSERVATIONS ON ANTIBODY-PRODUCING LYMPH NODE CELLS

1966 ◽  
Vol 123 (1) ◽  
pp. 161-172 ◽  
Author(s):  
T. N. Harris ◽  
Klaus Hummeler ◽  
Susanna Harris
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Lymph Node ◽  
Golgi Apparatus ◽  
Plasma Cells ◽  
Single Cells ◽  
Electron Microscopic ◽  
Lymph Node Cells ◽  
Considerable Range ◽  
Current Terminology

Lymph node cells of rabbits injected with sheep erythrocytes, identified as antibody-producing by their ability to produce plaques of hemolysis in erythrocyte-containing agar layers, have been examined by electron microscopy, by the use of a procedure devised for subjecting single cells to such examination. The antibody-producing cells thus examined were found to fall into two classes, according to the current terminology: some were in the category of lymphocytes, and others, in the category of plasma cells. Within each class, cells were found to vary in certain characteristics, especially in the degree of development of such organelles as the nucleolus, Golgi apparatus, and the endoplasmic reticulum. In the case of the endoplasmic reticulum especially, it could be seen that a series of these plaque-producing cells, ranked in order of increasing size and development of the endoplasmic reticulum, would extend over a considerable range from those lymphocytes with the least developed organelles to the mature plasma cells with the greatest development of these structures.

Ultrastructural modification of cellular interactions in cancer tumor

1978 ◽  
Vol 36 (2) ◽  
pp. 318-319
Author(s):  
N. P. Dmitrieva
Keyword(s):  
Cancer Cells ◽  
Human Thyroid ◽  
Adjacent Cell ◽  
Main Role ◽  
Cellular Interactions ◽  
Electron Microscopic ◽  
Cancer Tumor ◽  
Normal Human ◽  
Characteristic Features

One of the most characteristic features of cancer cells is their ability to metastasia. It is suggested that the modifications of the structure and properties of cancer cells surfaces play the main role in this process. The present work was aimed at finding out what ultrastructural features apear in tumor in vivo which removal of individual cancer cells from the cell population can provide. For this purpose the cellular interactions in the normal human thyroid and cancer tumor of this gland electron microscopic were studied. The tissues were fixed in osmium tetroxide and were embedded in Araldite-Epon.In normal human thyroid the most common type of intercellular contacts was represented by simple junction formed by the parallelalignment of adjacent cell membranees leaving in between an intermembranes space 15-20 nm filled with electronlucid material (Fig. 1a). Sometimes in the basal part of cells dilatations of the intercellular space 40-50 nm wide were found (Fig. 1a). Here the cell surfaces may form single short microvilli.

HUMAN LYMPH-NODE-CELL TRANSFORMATION

The Lancet ◽  
1967 ◽  
Vol 289 (7489) ◽  
pp. 579 ◽  
Author(s):  
C.A Rubio ◽  
J Zajicek
Keyword(s):  
Lymph Node ◽  
Cell Transformation ◽  
Lymph Node Cell ◽  
Human Lymph Node

scholarly journals "Intermediate" Cell Types and Mixed Cell Proliferation in Multiple Myeloma: Electron Microscopic Observations

Blood ◽  
1966 ◽  
Vol 27 (2) ◽  
pp. 212-226 ◽  
Author(s):  
JORGE E. MALDONADO ◽  
ROBERT A. KYLE ◽  
ARNOLD L. BROWN ◽  
EDWIN D. BAYRD
Keyword(s):  
Multiple Myeloma ◽  
Cell Proliferation ◽  
Plasma Cells ◽  
Cell Transformation ◽  
Cell Types ◽  
Lymphoid Cells ◽  
Intermediate Cell ◽  
Electron Microscopic ◽  
Mixed Cell ◽  
Reticulum Cells

Abstract Bone marrow studies of multiple myeloma revealed, in some cases, a conspicuous proliferation of "lymphoid" cells, virtually indistinguishable by light microscopy from those seen in lympho-proliferative disorders. Electron microscopy demonstrated a variety of cells ranging from typical lymphocytes to cells with plasmocytoid features. Between these two types of elements there were cells with intermediate characteristics. In addition, in several cases of myeloma the presence of fixed reticuloendothelial cells and "reticular" plasma cells (or reticulum cells with plasmocytic features) was frequently noted. The presence of reticulum cells and lymphocytes and of cells apparently "intermediate" between these cellular elements and plasma cells, as judged from electron microscopic observations, is suggestive morphologic evidence of a phenomenon of cell transformation and evidence of a mixed cell proliferation in certain cases of multiple myeloma.

scholarly journals ANTIGEN RECOGNITION: IN VITRO STUDIES ON THE SPECIFICITY OF THE CELLULAR IMMUNE RESPONSE

1969 ◽  
Vol 130 (5) ◽  
pp. 1031-1045 ◽  
Author(s):  
Stuart F. Schlossman ◽  
Judith Herman ◽  
Arieh Yaron
Keyword(s):  
Immune Response ◽  
Lymph Node ◽  
Cellular Immune Response ◽  
Lymph Node Cell ◽  
Antigen Receptors ◽  
Lymph Node Cells ◽  
Cellular Immune ◽  
Conventional Antibody ◽  
Sensitive Population

Studies of the immunochemical specificity of antigen-induced thymidine-2-14C incorporation in lymph node cells obtained from animals immunized to a series of closely related α-DNP-oligolysines, ϵ-DNP-oligolysines, and oligolysines have shown that the sensitized cell exhibits an extraordinary degree of specificity for antigen. The sensitized cell is maximally stimulated by the homologous immunizing antigen and can discriminate among compounds which differ from one another only in the position of a dinitrophenyl group or D-lysine residue on an identical oligolysine backbone. These studies support the view that the immunogen is not degraded prior to the induction of the immune response, and that the majority of cells produced as a consequence of immunization have stereospecific antigen receptors for the DNP-oligolysine used to induce the response; a smaller and more variably sized population of cells is produced with receptors specific for the oligolysine portion of the immunizing antigen. When specifically sensitized lymph node cell cultures are stimulated in vitro by heterologous DNP-oligolysines, the oligolysine- and not the DNP-oligolysine-sensitive population of cells appears to play a crucial role in the specificity of such cross-reactions. It is concluded from these studies that the antigen receptor on the sensitized lymph node cell differs in both kind and degree from conventional antibody. The chemical nature of the receptor and the means by which this receptor reacts with antigen to initiate the biosynthetic or proliferative cellular immune response still remain undefined.

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