Light microscopic visualization of colloidal gold on resin-embedded tissue.
1983 ◽
Vol 31
(12)
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pp. 1394-1398
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Keyword(s):
RNase labeled with colloidal gold was used as a model for the present technique evolved for the light microscopic localization of gold-labeled substances in semithin resin-embedded sections. Tissue sections placed on glass slides were treated with the gold-enzyme complex and subsequently exposed to a photographic developed containing silver lactate. During the development gold particles are encapsulated in growing shells of metallic silver and gradually made visible in the light microscope. The amplification method can be applied to paraffin-embedded and frozen sections as well. This technique may prove useful as a supplement to studies utilizing colloidal gold or silver as markers normally used at the electron microscopic level.
1989 ◽
Vol 37
(12)
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pp. 1799-1809
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Immunoelectron microscopic localization of estrogen receptor with monoclonal estrophilin antibodies.
1985 ◽
Vol 33
(9)
◽
pp. 915-924
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1978 ◽
Vol 36
(2)
◽
pp. 64-65
1985 ◽
Vol 43
◽
pp. 468-469
1986 ◽
Vol 34
(6)
◽
pp. 785-793
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1962 ◽
Vol 12
(2)
◽
pp. 385-410
◽
2003 ◽
Vol 51
(1)
◽
pp. 31-39
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