Rapid method for identification of macrophages in suspension by acid alpha-naphthyl acetate esterase activity.
1983 ◽
Vol 31
(7)
◽
pp. 960-963
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Keyword(s):
Azo Dye
◽
A supravital staining procedure for the identification of macrophages in cell suspension using a modification of a standard cytochemical assay for alpha-naphthyl acetate esterase (ANAE) activity is described. Macrophages are stained an intense red-brown after 5 min incubation in a buffer using ANAE as the substrate and hexazonium pararosaniline as the coupler for the azo dye. There is close agreement in the number of ANAE-positive cells found and the number of macrophages identified in smears by morphological criteria, by phagocytosis, and by the presence of Fc receptors. Therefore, this stain provides a quick, inexpensive method to estimate the number of macrophages present in suspensions of lymphocytic tissues from rats and mice.
1987 ◽
Vol 43
(1)
◽
pp. 92-96
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1983 ◽
Vol 4
(4)
◽
pp. 505-512
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Keyword(s):