scholarly journals Ultrastructural differentiation within the central nervous system between indolamines and catecholamines by energy dispersive X-ray analysis following paraformaldehyde pretreatment.

1983 ◽  
Vol 31 (8) ◽  
pp. 1005-1009 ◽  
Author(s):  
R E McClung ◽  
J Wood
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Substantia Nigra ◽  
Energy Dispersive ◽  
X Ray ◽  
Endogenous Dopamine ◽  
Dense Bodies ◽  
The Central Nervous System ◽  
Selective Localization ◽  
Raphe Neurons

Dense bodies containing high amounts of chrome were localized in the perikarya of substantia nigra and dorsal raphe neurons following the cytochemical reaction of endogenous dopamine and serotonin (respectively) with glutaraldehyde-dichromate (GDC). Energy dispersive X-ray analysis of these bodies revealed chrome levels two to four times higher than those recorded from the cytoplasmic background. Pretreatment with paraformaldehyde blocked the GDC reaction within the dense bodies in the substantia nigra (chrome levels similar to background), while the chrome levels in the dense bodies of the raphe neurons remained elevated. This demonstrates that pretreatment with paraformaldehyde allows selective localization of central nervous system serotonin stores by the GDC technique.

scholarly journals Acid-beta-glycerophosphatase reaction products in the central nervous system mitochondria following x-ray irradiation.

1975 ◽  
Vol 23 (6) ◽  
pp. 402-410 ◽  
Author(s):  
L Roizin ◽  
D Orlovskaja ◽  
J C Liu ◽  
A L Carsten
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Acid Phosphatase ◽  
Enzyme Reaction ◽  
Reaction Products ◽  
Huntington's Chorea ◽  
X Ray ◽  
System A ◽  
Chemical Lesion ◽  
The Central Nervous System

A survey of the literature to date on the enzyme histochemistry of intracellular organelles has not yielded any reference to the presence of acid phosphatase reaction products in the mammalian mitochondria of the central nervous system. A combination of Gomori's acid phosphatase mehtod, however, with standard electron microscopy has disclosed the presence of enzyme reaction products in the mitochondria of the central nervous system of rats from 2 hr to 22 weeks after x-ray irradiation, as well as in a cerebral biopsy performed on a patient affected by Huntington's chorea. No enzyme reaction products, on the other hand, were observed in serial sections that had been incubated in substrates either containing sodium fluoride or lacking in beta-glycerophosphate. The abnormal mitochondrial enzyme reaction (chemical lesion) is considered to be the consequenco of the pathologic process affecting the ultrastructural-chemical organization of the organelle.

The practical value of the method of indirect X-ray therapy for certain skin diseases

1936 ◽  
Vol 32 (7) ◽  
pp. 849-852
Author(s):  
F. C. Epstein ◽  
V. O. Lushchitsky
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Autonomic Nervous System ◽  
Inflammatory Process ◽  
Skin Diseases ◽  
Sebaceous Glands ◽  
X Ray ◽  
Inflammatory Processes ◽  
The Central Nervous System ◽  
Experimental Works

The mechanism of action of X-ray irradiation on the course of certain skin diseases, especially those whose etiology is unknown, is not yet entirely clear. There is no doubt that the autonomic nervous system, functionally and anatomically connected both with the central nervous system and with all organs and tissues, and in particular with the skin (its vessels, sweat and sebaceous glands), has an effect on all functions of the skin, its trophism and course inflammatory processes. Although the details of this effect on the inflammatory process have not been clarified and are still very contradictory, nevertheless a number of experimental works of old and recent times indicate an intimate dependence of the inflammatory process in the skin on the nervous system (Spiess, Thomas, Luvis, Speransky, Greenstein, Alperin and others).

Presence of melatonin in various cat brainstem nuclei determined by radioimmunoassay

1982 ◽  
Vol 101 (2) ◽  
pp. 161-165 ◽  
Author(s):  
M. Sallanon ◽  
B. Claustrat ◽  
M. Touret
Keyword(s):  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Substantia Nigra ◽  
Brain Tissue ◽  
Local Synthesis ◽  
Locus Caeruleus ◽  
Brainstem Nuclei ◽  
Cat Brain ◽  
The Central Nervous System

Abstract. Microdissected samples of juvenile cat brain tissue were assayed for melatonin content using a double antibody radioimmunoassay. Immunoreactive melatonin was consistently detected, albeit in variable amounts, in pineal, habenula, the region of the nucleus gracilis, gigantocellular reticular formation of the pons and medulla oblongata. Among the negative areas were raphe nuclei, substantia nigra and locus caeruleus. These findings suggest that melatonin may play a role in some structures of the central nervous system outside the pineal-hypothalamo-pituitary axis. This immunoreactive melatonin could reflect a local synthesis, or a tissular uptake of melatonin from blood or cerebrospinal fluid.

Cyclization of 1-{[(4-Methyl-4H-1,2,4-triazol-3-yl)sulfanyl]acetyl}thiosemicarbazides to 1,2,4-Triazole and 1,3,4-Thiadiazole Derivatives and Their Pharmacological Properties

2005 ◽  
Vol 70 (1) ◽  
pp. 51-62 ◽  
Author(s):  
Alicja Maliszewska-Guz ◽  
Monika Wujec ◽  
Monika Pitucha ◽  
Maria Dobosz ◽  
Anna Chodkowska ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Aqueous Solution ◽  
Central Nervous System ◽  
Nervous System ◽  
Pharmacological Properties ◽  
Ethyl Bromoacetate ◽  
Acid Media ◽  
X Ray ◽  
The Central Nervous System ◽  
Thiadiazole Derivatives

By the reaction of 4-methyl-4H-1,2,4-triazole-3-thiol with ethyl bromoacetate, ethyl [(4-methyl-4H-1,2,4-triazol-3-yl)sulfanyl]acetate (1) was obtained. This compound was converted to [(4-methyl-4H-1,2,4-triazol-3-yl)sulfanyl]acetohydrazide (2). In the reaction of 2 with isothiocyanates, new thiosemicarbazides 3a-3g were obtained. The cyclization of 3a-3g in 2% aqueous solution of sodium hydroxide led to the formation of 4H-1,2,4-triazole-3(2H)-thione derivatives 4a-4g, whereas the cyclization in acid media led to the formation of 2-amino-1,3,4-thiadiazole derivatives 5a-5g. Molecular structure was confirmed by X-ray structure analysis of 3a, 4g, 5a and 5g. Compounds 4a, 4b and 4g were investigated pharmacologically to determine their effect on the central nervous system (CNS) in mice.

The use of X-rays and radium beams in the therapy of nervous diseases

1930 ◽  
Vol 26 (9) ◽  
pp. 946-946
Author(s):  
R. Gasul
Keyword(s):  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Radiation Therapy ◽  
X Rays ◽  
Pathological Changes ◽  
X Ray ◽  
Retarding Effect ◽  
Plexus Chorioideus ◽  
The Central Nervous System

Kazan Society of Radiologists and Radiologists. Joint meeting with the O vom of neuropathologists 27 / XI 1929 R. Gasul. The use of X-rays and radium beams in the therapy of nervous diseases. Having reported on the first experiments on the effect of rays on the nervous system of Tarkhanov (USSR), Rodet and Bertin (France), Obersteiner and Dеmel (Germany), the speaker described those pathological changes in the central nervous system under the influence of X-ray and radium rays, which lie in the basis of modern radiation therapy in a nervous clinic. Special attention was paid by the speaker to the retarding effect of the drug on the production of cerebrospinal fluid in the plexus chorioideus and the analgesic effect of X-rays and radium rays.

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