scholarly journals CHARACTERIZATION OF SCHIFF-POSITIVE SUBSTANCES IN ELASTIC FIBERS

1958 ◽  
Vol 6 (4) ◽  
pp. 260-264 ◽  
Author(s):  
FRANK S. LABELLA
Keyword(s):  
Enzymatic Hydrolysis ◽  
Positive Reaction ◽  
Mercuric Chloride ◽  
Staining Intensity ◽  
Elastic Fibers ◽  
Fatty Material ◽  
Extraction Techniques ◽  
Sulfurous Acid ◽  
Unsaturated Component

Schiff-positive substances in clastic fibers have been separated on the basis of differences in reactivity with the reagent. Alkali-treated ligamentum nuchae powder contains a cerebroside which is removed by chloroform or pyridine. Also present are a plasmalogen-like compound which depends upon its unsaturated component for its reactivity, and a substance whose Schiff positivity is negated by prior treatment with iodine; both reactants are resistant to extraction techniques. Mercuric chloride or enzymatic hydrolysis releases an unsaturated fatty material, probably the aldehydic component of the clastin-bound plasmalogen-like compound. Elastin powder which has been treated with phenylhydrazine, iodine and bromine still gives a positive reaction with fuchsin-sulfurous acid. Rat aortic clastica, like ligamentum nuchac, contains a non-extractable plasmalogen like compound whose reactivity with Schiff's reagent depends Upon unsaturation in the molecule. Staining intensity is reduced by prior chloroform extraction, suggesting a glycolipid as was demonstrated for ligamentum nuchae.

Characterization of Physical, Mechanical, and Biological Properties of SilkBridge Nerve Conduit after Enzymatic Hydrolysis

2020 ◽  
Vol 3 (12) ◽  
pp. 8361-8374
Author(s):  
Silvia Biggi ◽  
Giulia A. Bassani ◽  
Valentina Vincoli ◽  
Daniele Peroni ◽  
Valerio Bonaldo ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Biological Properties ◽  
Nerve Conduit

scholarly journals Extraction Techniques and Analytical Methods for Characterization of Active Compounds in Origanum Species

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4735
Author(s):  
Maša Knez Hrnčič ◽  
Darija Cör ◽  
Jana Simonovska ◽  
Željko Knez ◽  
Zoran Kavrakovski ◽  
...  
Keyword(s):  
Biological Activity ◽  
Abiotic Stresses ◽  
Plant Protection ◽  
Analytical Techniques ◽  
Extraction Techniques ◽  
Isolation And Identification ◽  
Activity Assessment ◽  
Current Contribution ◽  
Pharmaceutical Industries

Phytochemical research based on ethnopharmacology is gaining interest in industries such as functional food, nutraceuticals, cosmetics and pharmaceutical industries. Plants and plant extracts are a rich source of bioactive secondary metabolites. These compounds are often involved in plant protection against biotic or abiotic stresses. The exploitation of available technologies should be oriented and intensified to extend and enhance the continued usefulness of the plants as renewable sources of chemicals, especially medicinal compounds. This current contribution is focused on extraction and analytical techniques for their isolation from the oregano species, their characterization and their potential antioxidative, as well as their antimicrobial, antifungal and anticarcinogenic properties. The work is structured rendering to the different steps involved in the research; starting with extraction and sample preparation, followed by discussing the analytical techniques employed for the isolation and identification of compound/s responsible for the biological activity and methods and techniques for biological activity assessment.

scholarly journals Synthesis and spectral characterization of 2,2-diphenylethyl glucosinolate and HPLC-based reaction progress curve data for the enzymatic hydrolysis of glucosinolates by Sinapis alba myrosinase

Data in Brief ◽  
2017 ◽  
Vol 10 ◽  
pp. 151-181 ◽  
Author(s):  
Chase A. Klingaman ◽  
Matthew J. Wagner ◽  
Justin R. Brown ◽  
John B. Klecker ◽  
Ethan H. Pauley ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sinapis Alba ◽  
Spectral Characterization ◽  
Reaction Progress ◽  
Progress Curve ◽  
Hydrolysis Of

scholarly journals Intermediate filaments in non-neuronal cells of invertebrates: isolation and biochemical characterization of intermediate filaments from the esophageal epithelium of the mollusc Helix pomatia.

1985 ◽  
Vol 101 (2) ◽  
pp. 427-440 ◽  
Author(s):  
E Bartnik ◽  
M Osborn ◽  
K Weber
Keyword(s):  
Positive Reaction ◽  
Intermediate Filaments ◽  
Biochemical Characterization ◽  
Helix Pomatia ◽  
Molar Ratio ◽  
Negative Stain ◽  
Epithelial Morphology ◽  
Lower Chordate

To screen invertebrate tissues for the possible expression of intermediate filaments (IFs), immunofluorescence microscopy with the monoclonal antibody anti-IFA known to detect all mammalian IF proteins was used (Pruss, R. M., R. Mirsky, M. C. Raff, R. Thorpe, A. J. Dowding, and B. H. Anderton. 1981. Cell, 27:419-428). In a limited survey, the lower chordate Branchiostoma as well as the invertebrates Arenicola, Lumbricus, Ascaris, and Helix pomatia revealed a positive reaction primarily on epithelia and on nerves, whereas certain other invertebrates appeared negative. To assess the nature of the positive reaction, Helix pomatia was used since a variety of epithelia was strongly stained by anti-IFA. Fixation-extraction procedures were developed that preserve in electron micrographs of esophagus impressive arrays of IFs as tonofilament bundles. Fractionation procedures performed on single cell preparations document large meshworks of long and curvilinear IF by negative stain. These structures can be purified. One- and two-dimensional gels show three components, all of which are recognized by anti-IFA in immunoblotting: 66 kD/pl 6.35, 53 kD/pl 6.05, and 52 kD/pl 5.95. The molar ratio between the larger and more basic polypeptide and the sum of the two more acidic forms is close to 1. After solubilization in 8.5 M urea, in vitro filament reconstitution is induced when urea is removed by dialysis against 2-50 mM Tris buffer at pH 7.8. The reconstituted filaments contain all three polypeptides. The results establish firmly the existence of invertebrate IFs outside neurones and demonstrate that the esophagus of Helix pomatia displays IFs which in line with the epithelial morphology of the tissue could be related to keratin IF of vertebrates.

Enzymatic hydrolysis and characterization of lignocellulosic biomass exposed to electron beam irradiation

2012 ◽  
Vol 90 (2) ◽  
pp. 1038-1045 ◽  
Author(s):  
K. Karthika ◽  
A.B. Arun ◽  
P.D. Rekha
Keyword(s):  
Electron Beam ◽  
Enzymatic Hydrolysis ◽  
Lignocellulosic Biomass ◽  
Electron Beam Irradiation ◽  
Beam Irradiation

Rheological and structural characterization of whey protein gelation induced by enzymatic hydrolysis

2016 ◽  
Vol 61 ◽  
pp. 211-220 ◽  
Author(s):  
Ozgur Tarhan ◽  
Maria Julia Spotti ◽  
Sam Schaffter ◽  
Carlos M. Corvalan ◽  
Osvaldo H. Campanella
Keyword(s):  
Enzymatic Hydrolysis ◽  
Whey Protein ◽  
Structural Characterization ◽  
Protein Gelation

Characterization of Organic Phosphorus Form and Bioavailability in Lake Sediments using 31 P Nuclear Magnetic Resonance and Enzymatic Hydrolysis

2015 ◽  
Vol 44 (3) ◽  
pp. 882-894 ◽  
Author(s):  
Courtney D. Giles ◽  
Lydia G. Lee ◽  
Barbara J. Cade-Menun ◽  
Jane E. Hill ◽  
Peter D. F. Isles ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Enzymatic Hydrolysis ◽  
Lake Sediments ◽  
Organic Phosphorus ◽  
Phosphorus Form ◽  
Nuclear Magnetic

Elemental characterization of coal ash and its leachates using sequential extraction techniques

1995 ◽  
Vol 192 (2) ◽  
pp. 265-274 ◽  
Author(s):  
S. Landsberger ◽  
J. F. Cerbus ◽  
S. Larson
Keyword(s):  
Sequential Extraction ◽  
Coal Ash ◽  
Extraction Techniques ◽  
Elemental Characterization
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