Platelet-associated autoantibodies as detected by a solid-phase modified antigen capture ELISA test (MACE) are a useful prognostic factor in idiopathic thrombocytopenic purpura

Blood ◽  
2004 ◽  
Vol 103 (12) ◽  
pp. 4562-4564 ◽  
Author(s):  
Fabrizio Fabris ◽  
Raffaella Scandellari ◽  
Elisabetta Ruzzon ◽  
Maria Luigia Randi ◽  
Guido Luzzatto ◽  
...  
Keyword(s):  
Idiopathic Thrombocytopenic Purpura ◽  
Clinical Course ◽  
Solid Phase ◽  
Elisa Test ◽  
Patient Admission ◽  
Enzyme Linked Immunosorbent Assay ◽  
Thrombocytopenic Purpura ◽  
Antigen Capture ◽  
Clinical Worsening ◽  
Platelet Autoantibodies

Abstract There were 50 consecutive idiopathic thrombocytopenic purpura (ITP) adult patients (platelet count < 100 × 109/L) grouped according to positivity or negativity of a solid-phase modified antigen capture enzyme-linked immunosorbent assay (ELISA) test (MACE) against glycoprotein IIb/IIIa (GPIIb/IIIa), Ib/IX, and IIa/IIIa. Observation started on the day of MACE assay and lasted at least 6 months. Clinical worsening was defined as the need for starting or modifying therapy because of thrombocytopenia lower than 20 × 109/L or patient admission due to bleeding symptoms. MACE-positive patients had a higher probability of clinical worsening than MACE-negatives (P < .004). The proportion of patients worsening was 18 (72%) of 25 among MACE-positives and 8 (32%) of 25 among MACE-negatives. The median time to clinical worsening was 2.1 months for MACE-positive patients and 27.7 months for MACE-negatives. The assay of specific platelet autoantibodies may be a useful prognostic tool for the clinical course of ITP. (Blood. 2004;103:4562-4564)

scholarly journals Evaluation by quantitative acid elution and radioimmunoassay of multiple classes of immunoglobulins and serum albumin associated with platelets in idiopathic thrombocytopenic purpura

Blood ◽  
1986 ◽  
Vol 67 (4) ◽  
pp. 1126-1131 ◽  
Author(s):  
AJ Hotchkiss ◽  
CA Leissinger ◽  
ME Smith ◽  
JV Jordan ◽  
CA Kautz ◽  
...  
Keyword(s):  
Serum Albumin ◽  
Idiopathic Thrombocytopenic Purpura ◽  
Solid Phase ◽  
Similar Group ◽  
Thrombocytopenic Purpura ◽  
Large Numbers ◽  
Wide Range ◽  
Platelet Concentration ◽  
Associated Proteins ◽  
Solid Phase Radioimmunoassay

Abstract Immunoglobulins (Igs) and serum albumin were eluted from normal platelets and platelets from patients with idiopathic thrombocytopenic purpura (ITP) with a quantitative acid elution procedure followed by solid-phase radioimmunoassay (SPRIA). Acid elution was shown to release a reproducible fraction of platelet-associated Igs, and the amounts released per platelet were independent of the platelet concentration over a wide range of concentrations. This procedure is suitable for sensitive, reproducible, and specific quantitation of large numbers of samples. Washed platelets from 13 normal donors contained the following components (expressed in femtograms per platelet, mean +/- 2 SEM): IgG, 1.40 +/- 0.26; IgA, 0.72 +/- 0.36; IgM 0.078 +/- 0.036; albumin 7.7 +/- 1.5. Immunoglobulins and albumin eluted from the platelets of ten ITP patients (two in remission), expressed as femtograms per platelet, mean (range), were: IgG 104 (0.3 to 750); IgA 90 (0.9 to 715); IgM 162 (1.2 to 1,300); and albumin 34 (6.8 to 199). All platelet-associated Igs from thrombocytopenic ITP patients were found to be elevated twofold to 2,300-fold with one Ig class occasionally elevated 50-fold to 100-fold higher than the others. A similar group of ten thrombocytopenic ITP patients was found to have twofold to 26-fold elevations of platelet- associated albumin. This demonstration of increases in multiple classes of Igs as well as serum albumin associated with platelets from ITP patients suggests that some nonimmune process may be contributing to the phenomenon of increased platelet-associated proteins in ITP.

Regulation of Serum Thrombopoietin Levels by Platelets and Megakaryocytes in Patients with Aplastic Anaemia and Idiopathic Thrombocytopenic Purpura

1996 ◽  
Vol 76 (02) ◽  
pp. 156-160 ◽  
Author(s):  
Naoaki Ichikawa ◽  
Fumihiro Ishida ◽  
Shigetaka Shimodaira ◽  
Tomoyuki Tahara ◽  
Takashi Kato ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Platelet Count ◽  
Aplastic Anaemia ◽  
Idiopathic Thrombocytopenic Purpura ◽  
Immunosorbent Assay ◽  
Regulatory Mechanism ◽  
Inverse Correlation ◽  
Normal Subjects ◽  
Enzyme Linked Immunosorbent Assay ◽  
Thrombocytopenic Purpura

SummaryTo clarify the regulatory mechanism of thrombopoietin (TPO, c-Mpl ligand) in chronic thrombocytopenic conditions, we determined TPO levels in the sera of patients with aplastic anaemia (AA; n = 26) and idiopathic thrombocytopenic purpura (ITP; n = 32) by an enzyme-linked immunosorbent assay. Despite a similarity in platelet counts, serum TPO levels in the AA group were markedly higher than those in the ITP group: 20.41 ± 9.71 f mol/ml (mean ± SD) and 1.66 ± 0.55 f mol/ml, respectively, both of which were significantly elevated compared to normal subjects (n = 41; 1.22 ± 0.37). In both groups, serum TPO level showed an inverse correlation with the platelet count. We determined the megakaryocyte volume using bone marrow clot section and found that it was markedly small in the AA group; while in the ITP group it was augmented with a correlation to serum TPO level. Our findings suggest that TPO levels may be regulated not only by platelets but also megakaryocytes in AA and ITP.

scholarly journals Microcystins Presence in Mussels(M. galloprovincialis)and Water of Two Productive Mediterranean’s Lagoons (Sardinia, Italy)

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Elena Baralla ◽  
Maria Vittoria Varoni ◽  
Tiziana Sedda ◽  
Valeria Pasciu ◽  
Antonello Floris ◽  
...  
Keyword(s):  
Public Health ◽  
Mass Spectrometry ◽  
Extraction Method ◽  
Mytilus Galloprovincialis ◽  
Solid Phase ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Phase Extraction ◽  
Mass Spectrometry Detection ◽  
The World

Microcystins (MCs) are hepatotoxins harmful for animal and human health. The most toxic type between them is MC-LR whose presence has been investigated in different reservoirs all around the world. In this work microcystins were monitored in spring and summer in water and mussels(Mytilus galloprovincialis)of two Sardinia lagoons: Cabras and Calich lagoons. A Solid Phase Extraction method was developed to clean and concentrate samples before the Enzyme Linked Immunosorbent Assay (ELISA) and the following Mass Spectrometry detection. MCs presence was detected using the screening ELISA test in both lagoons. MCs peak was revealed in July for water and mussels belonging to Cabras lagoon (0.75±0.07 ng/L in water and0.12±0.04 ng/g ww in mussels). In water of Calich lagoon there was a constant trend in the concentration of MCs during the considered months, while there was a MCs peak in July (0.6±0.5 ng/g ww) in mussels. The following LC-MS/MS analysis did not reveal MC-LR presence in all analyzed samples. These results can be useful to enrich knowledge on public health and consumer’s safeguard.

Selectins and IL-6 during the Clinical Course of Idiopathic Thrombocytopenic Purpura

1999 ◽  
Vol 101 (1) ◽  
pp. 16-20 ◽  
Author(s):  
İbrahim C. Haznedaroglu ◽  
Yahya Büyükaşık ◽  
Ali Koşar ◽  
Osman İ. Özcebe ◽  
Şerafettin Kirazlı ◽  
...  
Keyword(s):  
Idiopathic Thrombocytopenic Purpura ◽  
Clinical Course ◽  
Thrombocytopenic Purpura

scholarly journals Serological evaluation for the current epidemic situation of foot and mouth disease among cattle and buffaloes in Egypt

2020 ◽  
Vol 13 (1) ◽  
pp. 1-9
Author(s):  
Mariam M. Abd El-Rhman ◽  
Diea G. Abo El-Hassan ◽  
Walid S. Awad ◽  
Sayed A. H. Salem
Keyword(s):  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Elisa Test ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Structural Protein ◽  
Fmdv Serotypes ◽  
Foot And Mouth ◽  
Trivalent Vaccine

Aim: The present study was aimed to investigate the epidemic situation of foot-and-mouth disease (FMD) in Egypt from 2016 to 2018 based on the detection of FMD virus (FMDV) in carrier or previously infected animals, by determination of antibodies against non-structural protein (NSP), implementation a pilot study on circulating FMDV serotypes and assure the efficacy of locally produced inactivated trivalent vaccine. Materials and Methods: A total of 1500 sera were collected from apparent healthy vaccinated cattle and buffaloes from three Egyptian geographical sectors, representing ten governorates. Determination of FMD antibodies against NSP was carried out using 3ABC enzyme-linked immunosorbent assay (ELISA) test. Serotyping of the circulating FMDV and assure the vaccine efficacy was performed using solid-phase competitive ELISA. Results: The 3ABC ELISA test revealed 26.4% and 23.7% positive for FMDV-NSP antibodies in cattle and buffalo sera, respectively. The highest positivity was in Delta Sector among both cattle 42.3% and buffaloes 28.8%. Serotyping of FMDV-positive NSP sera in El-Qalyubia Governorate for the circulating FMDV serotypes O, A, and Southern African Territories (SAT) 2 was 52.2%, 17.4%, and 30.4% in cattle and 31.8%, 27.3%, and 40.9% in buffaloes, respectively. The overall protection level due to the vaccination program was 62.1 and 60.9% in cattle and buffaloes, respectively, while the protective level of the FMDV serotypes O, A, and SAT2 included in the inactivated trivalent vaccine was 73.9, 84.6, and 63.8% in cattle and 72.3, 82.3, and 63.5% in buffaloes, respectively. Conclusion: The present study recommended full determination for the immunogenic relationship between the vaccine strains and the field strains to attain maximum protection against the circulating viruses.

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