scholarly journals Ets1 and Ets2 are required for endothelial cell survival during embryonic angiogenesis

Blood ◽  
2009 ◽  
Vol 114 (5) ◽  
pp. 1123-1130 ◽  
Author(s):  
Guo Wei ◽  
Ruchika Srinivasan ◽  
Carmen Z. Cantemir-Stone ◽  
Sudarshana M. Sharma ◽  
Ramasamy Santhanam ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Target Genes ◽  
Cell Function ◽  
Endothelial Cell Function ◽  
Cell Functions ◽  
And Function ◽  
Target Promoters ◽  
Vascular Branching

Abstract The ras/Raf/Mek/Erk pathway plays a central role in coordinating endothelial cell activities during angiogenesis. Transcription factors Ets1 and Ets2 are targets of ras/Erk signaling pathways that have been implicated in endothelial cell function in vitro, but their precise role in vascular formation and function in vivo remains ill-defined. In this work, mutation of both Ets1 and Ets2 resulted in embryonic lethality at midgestation, with striking defects in vascular branching having been observed. The action of these factors was endothelial cell autonomous as demonstrated using Cre/loxP technology. Analysis of Ets1/Ets2 target genes in isolated embryonic endothelial cells demonstrated down-regulation of Mmp9, Bcl-XL, and cIAP2 in double mutants versus controls, and chromatin immunoprecipitation revealed that both Ets1 and Ets2 were loaded at target promoters. Consistent with these observations, endothelial cell apoptosis was significantly increased both in vivo and in vitro when both Ets1 and Ets2 were mutated. These results establish essential and overlapping functions for Ets1 and Ets2 in coordinating endothelial cell functions with survival during embryonic angiogenesis.

scholarly journals Methylglyoxal Impairs Brain Microvascular Endothelial Cell Function In Vivo and In Vitro

2009 ◽  
Vol 96 (3) ◽  
pp. 682a
Author(s):  
Aydin Tay ◽  
William G. Mayhan ◽  
Denise Arrick ◽  
Chun-Hong Shao ◽  
Hong Sun ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Function ◽  
Microvascular Endothelial Cell ◽  
Brain Microvascular Endothelial Cell ◽  
Endothelial Cell Function ◽  
Microvascular Endothelial

Lymphocytic microparticles inhibit angiogenesis by stimulating oxidative stress and negatively regulating VEGF-induced pathways

2008 ◽  
Vol 294 (2) ◽  
pp. R467-R476 ◽  
Author(s):  
Chun Yang ◽  
Bupe R. Mwaikambo ◽  
Tang Zhu ◽  
Carmen Gagnon ◽  
Josiane Lafleur ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Function ◽  
Umbilical Vein ◽  
Ros Generation ◽  
Vegf Receptor ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Endothelial Cell Function

Recent studies have demonstrated that lymphocyte-derived microparticles (LMPs) impair endothelial cell function. However, no data currently exist regarding the contribution of LMPs in the regulation of angiogenesis. In the present study, we investigated the effects of LMPs on angiogenesis in vivo and in vitro and demonstrated that LMPs strongly suppressed aortic ring microvessel sprouting and in vivo corneal neovascularization. In vitro, LMPs considerably diminished human umbilical vein endothelial cell survival and proliferation in a concentration-dependent manner. Mechanistically, the antioxidants U-74389G and U-83836E were partially protective against the antiproliferative effects of LMPs, whereas the NADPH oxidase (NOX) inhibitors apocynin and diphenyleneiodonium significantly abrogated these effects. Moreover, LMPs increased not only the expression of the NOX subunits gp91phox, p22phox, and p47phox, but also the production of ROS and NOX-derived superoxide (O2−). Importantly, LMPs caused a pronounced augmentation in the protein expression of the CD36 antiangiogenic receptor while significantly downregulating the protein levels of VEGF receptor type 2 and its downstream signaling mediator, phosphorylated ERK1/2. In summary, LMPs potently suppress neovascularization in vivo and in vitro by augmenting ROS generation via NOX and interfering with the VEGF signaling pathway.

Abstract 363: Activin receptor-like kinase 1 (Alk1) ligands modulate endothelial cell function in vitro and in vivo.

2013 ◽  
Author(s):  
Gareth C. Davies ◽  
James A. Harper ◽  
Richard C. Sainson ◽  
Lee Brown ◽  
Grace Opoku-Ansah ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Function ◽  
Endothelial Cell Function ◽  
Activin Receptor ◽  
Receptor Like Kinase

The tetrapeptide AcSDKP, an inhibitor of primitive hematopoietic cell proliferation, induces angiogenesis in vitro and in vivo

Blood ◽  
2003 ◽  
Vol 101 (8) ◽  
pp. 3014-3020 ◽  
Author(s):  
Jian-Miao Liu ◽  
Françoise Lawrence ◽  
Milica Kovacevic ◽  
Jérôme Bignon ◽  
Evangelia Papadimitriou ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Function ◽  
Active Form ◽  
Chorioallantoic Membrane ◽  
Endothelial Cell Migration ◽  
Endothelial Cell Function ◽  
Angiogenic Response ◽  
Matrix Metalloproteinase 1

Abstract The tetrapeptide acetyl-Ser-Asp-Lys-Pro (AcSDKP), purified from bone marrow and constitutively synthesized in vivo, belongs to the family of negative regulators of hematopoiesis. It protects the stem cell compartment from the toxicity of anticancer drugs and irradiation and consequently contributes to a reduction in marrow failure. This current work provides experimental evidence for another novel biologic function of AcSDKP. We report that AcSDKP is a mediator of angiogenesis, as measured by its ability to modulate endothelial cell function in vitro and angiogenesis in vivo. AcSDKP at nanomolar concentrations stimulates in vitro endothelial cell migration and differentiation into capillary-like structures on Matrigel as well as enhances the secretion of an active form of matrix metalloproteinase-1 (MMP-1). In vivo, AcSDKP promotes a significant angiogenic response in the chicken embryo chorioallantoic membrane (CAM) and in the abdominal muscle of the rat. Moreover, it induces the formation of blood vessels in Matrigel plugs implanted subcutaneously in the rat. This is the first report demonstrating the ability of AcSDKP to interact directly with endothelial cells and to elicit an angiogenic response in vitro and in vivo.

Toll-like receptor 3 regulates cord blood-derived endothelial cell function in vitro and in vivo

Angiogenesis ◽  
2013 ◽  
Vol 16 (4) ◽  
pp. 821-836 ◽  
Author(s):  
Aurore Grelier ◽  
Audrey Cras ◽  
Nicole Balitrand ◽  
Catherine Delmau ◽  
Séverine Lecourt ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cord Blood ◽  
Cell Function ◽  
Toll Like Receptor ◽  
Endothelial Cell Function

scholarly journals The Ubiquitously Expressed Csk Adaptor Protein Cbp Is Dispensable for Embryogenesis and T-Cell Development and Function

2005 ◽  
Vol 25 (23) ◽  
pp. 10533-10542 ◽  
Author(s):  
Marc-Werner Dobenecker ◽  
Christian Schmedt ◽  
Masato Okada ◽  
Alexander Tarakhovsky
Keyword(s):  
T Cell ◽  
Adaptor Protein ◽  
Regulatory Function ◽  
Loss Of Function ◽  
Thymic Development ◽  
Cell Functions ◽  
Carboxy Terminal ◽  
And Function

ABSTRACT Regulation of Src family kinase (SFK) activity is indispensable for a functional immune system and embryogenesis. The activity of SFKs is inhibited by the presence of the carboxy-terminal Src kinase (Csk) at the cell membrane. Thus, recruitment of cytosolic Csk to the membrane-associated SFKs is crucial for its regulatory function. Previous studies utilizing in vitro and transgenic models suggested that the Csk-binding protein (Cbp), also known as phosphoprotein associated with glycosphingolipid microdomains (PAG), is the membrane adaptor for Csk. However, loss-of-function genetic evidence to support this notion was lacking. Herein, we demonstrate that the targeted disruption of the cbp gene in mice has no effect on embryogenesis, thymic development, or T-cell functions in vivo. Moreover, recruitment of Csk to the specialized membrane compartment of “lipid rafts” is not impaired by Cbp deficiency. Our results indicate that Cbp is dispensable for the recruitment of Csk to the membrane and that another Csk adaptor, yet to be discovered, compensates for the loss of Cbp.

Polyphyllin D, a steroidal saponin from Paris polyphylla, inhibits endothelial cell functions in vitro and angiogenesis in zebrafish embryos in vivo

2011 ◽  
Vol 137 (1) ◽  
pp. 64-69 ◽  
Author(s):  
Judy Yuet-Wa Chan ◽  
Johnny Chi-Man Koon ◽  
Xiaozhou Liu ◽  
Michael Detmar ◽  
Biao Yu ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Zebrafish Embryos ◽  
Steroidal Saponin ◽  
Cell Functions ◽  
Paris Polyphylla ◽  
Polyphyllin D

Enhanced Vascular Endothelial Cell Function on Nanostructured Titanium Surface Features: The Role of Nano to Submicron Roughness

2008 ◽  
Vol 1136 ◽  
Author(s):  
Jing Lu ◽  
Dongwoo Khang ◽  
Thomas J. Webster
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Cell Function ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Endothelial Cell Function ◽  
Titanium Surfaces ◽  
Endothelial Cell Adhesion

ABSTRACTTo study the contribution of different surface feature properties in improving vascular endothelial cell adhesion, rationally designed nano/sub-micron patterns with various dimensions were created on titanium surfaces in this study. In vitro results indicated that endothelial cell adhesion was improved when the titanium pattern dimensions decreased into the nano-scale. Specifically, endothelial cells preferred to adhere on sub-micron and nano rough titanium substrates compared to flat titanium. Moreover, titanium with nano and sub-micron roughness and with the same chemistry as compared to flat titanium, had significantly greater surface energy. Thus, the present study indicated the strong potential of surface nanotopography and nano/sub-micron roughness for improving current vascular stent design.

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