scholarly journals Microscopy of Living Bone Marrow In Situ

Blood ◽  
1971 ◽  
Vol 38 (1) ◽  
pp. 87-95 ◽  
Author(s):  
ROBERT S. MCCUSKEY ◽  
SAMUEL G. MCCLUGAGE ◽  
WALDO J. YOUNKER
Keyword(s):  
Bone Marrow ◽  
Microscopic Examination ◽  
Hemopoietic Cells ◽  
Highly Active ◽  
Preliminary Examination ◽  
Yellow Marrow ◽  
Living Bone

Abstract A chamber has been designed to permit chronic microscopic examination of living bone marrow in situ. The amount of metal adjacent to the tissue within the gap of the chamber appears to be critical if normal hemopoiesis is to occur, since excessive metal resulted in the regeneration of a hypocellular, gelatinous marrow. When the amount of metal was reduced, relatively normal marrow regenerated into the chamber. Most frequently this histologically resembled yellow marrow but on occasion highly active marrow was observed. Preliminary examination of the microvascular system confirmed previous reports that sinusoids generally were arranged in interconnecting polygonal networks surrounding individual or clusters of fat and hemopoietic cells. The sinusoids were lined by a definite endothelium. No "intersinusoidal capillaries" were observed.

scholarly journals In situ detection of individual transplanted bone marrow cells using FISH on sections of paraffin-embedded whole murine femurs.

1996 ◽  
Vol 44 (9) ◽  
pp. 1069-1074 ◽  
Author(s):  
S K Nilsson ◽  
R Hulspas ◽  
H U Weier ◽  
P J Quesenberry
Keyword(s):  
Bone Marrow ◽  
Y Chromosome ◽  
Cell Tracking ◽  
Fluorescent Dyes ◽  
Bone Marrow Cells ◽  
Ex Vivo ◽  
Flow Cytometric Analysis ◽  
Hemopoietic Cells ◽  
Depth Analysis

Studies of transplantation biology rely on the detection of donor hemopoietic cells in transplant recipients. Traditionally this has been achieved through ex vivo techniques, including flow cytometric analysis of cell surface markers to detect cells expressing specific epitopes, histochemical detection of cytoplasmic proteins, and the detection of Y chromosome-specific sequences by DNA hybridization. Studies using congenic models, such as the Ly5.1/5.2 mouse, or the utilization of fluorescent dyes, such as PKH-26, have allowed more in-depth analysis of transplantation, beginning to address key issues such as cell homing through cell tracking and elucidation of the "stem cell niche." However, these methods are limited by labeling sensitivity, specificity, crossreactivity and, in the case of PKH-26 labeling, the number of cell divisions the transplanted cells can make before the signal disappears. We have developed a fluorescent in situ hybridization (FISH) technique that utilizes a murine Y chromosome-specific "painting" probe to identify in situ individual transplanted male cells in paraffin-embedded sections of female whole bone marrow while maintaining good morphological integrity. This method is highly sensitive and specific, labeling more than 99% of male cells and no female cells, allowing each transplant to be assessed at the individual cell level. The technique provides unique opportunities to follow the path taken by transplanted cells, both during homing into the marrow and through their maturation and differentiation into mature, functional hemopoietic cells.

scholarly journals Microscopy of Living Bone Marrow In Situ. II. Influence of the Microenvironment on Hemopoiesis

Blood ◽  
1971 ◽  
Vol 38 (1) ◽  
pp. 96-107 ◽  
Author(s):  
SAMUEL G. MCCLUGAGE ◽  
ROBERT S. MCCUSKEY ◽  
HOWARD A. MEINEKE
Keyword(s):  
Bone Marrow ◽  
Intimate Relationship ◽  
Fat Cells ◽  
Proliferating Cells ◽  
Dynamic Changes ◽  
Cellular Compartment ◽  
Local Factors ◽  
Living Bone

Abstract The present study examined in vivo the dynamic changes of hypocellular bone marrow during increased hemopoietic activity induced by phlebotomy or administration of erythropoietin. During increased hemopoietic activity, large venules and venous sinusoids located within the marrow spaces were replaced by dilated or polygonal networks of sinusoids supplied by small arterioles or capillaries that coursed from surrounding spicules of bone into the marrow spaces. An increased cellularity (hemopoietic and fat cells) was associated with this change in vascularity. The results suggest the presence of two functional parts of the microvascular system in bone marrow. The first is associated with hemopoietic areas of the marrow and is erythropoietin-sensitive; the second, with regeneration and resorption of bone. The presence of cancellous bone and the morphology of the microvascular system may determine the extent of hemopoietic activity of bone marrow. During increased hemopoietic activity, the intimate relationship between the microvascular system, bone spicules, and the developing cellular compartment suggests local factors resident in bone are transported to these proliferating cells to exert a hemopoietic effect.

In Situ Activated Co3–xNixO4 as a Highly Active and Ultrastable Electrocatalyst for Hydrogen Generation

ACS Catalysis ◽  
2021 ◽  
pp. 8174-8182
Author(s):  
Kailu Guo ◽  
Yantao Wang ◽  
Junfeng Huang ◽  
Min Lu ◽  
Hua Li ◽  
...  
Keyword(s):  
Hydrogen Generation ◽  
Highly Active

scholarly journals An Unsuspected Finding of t(9;22): A Rare Case of Philadelphia Chromosome-Positive B-Lymphoblastic Lymphoma

2017 ◽  
Vol 2017 ◽  
pp. 1-4 ◽  
Author(s):  
Prajwal Boddu ◽  
C. Cameron Yin ◽  
Rashmi Kanagal-Shamanna ◽  
Guillin Tang ◽  
Beenu Thakral ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Kinase Inhibitors ◽  
Bone Marrow Involvement ◽  
Philadelphia Chromosome ◽  
Lymphoblastic Lymphoma ◽  
Accurate Diagnosis ◽  
Biopsy Specimens ◽  
B Lymphoblastic Lymphoma ◽  
Prophylactic Chemotherapy

While rare, cases of isolated extramedullary disease of B-cell Lymphoblastic Lymphoma (B-LBL) without morphologic bone marrow involvement have been described. In this report, we illustrate the case of an elderly gentleman who presented with isolated testicular and vertebral LBL involvement but had no morphologic bone marrow involvement. The initial plan of treatment was to treat along the lines of Philadelphia negative B-ALL/LBL. During this time, fluorescence in situ hybridization (FISH) and PCR testing for BCR-ABL1 rearrangements were being performed on the marrow specimens as a part of routine diagnostic workup. While the FISH returned negative, PCR testing unexpectedly detected BCR-ABL1 fusion transcripts at a low level of 0.48%. Given their presence, we performed FISH for BCR/ABL1 rearrangement in both testicular and L5 vertebral specimens which were 80–90% positive. He subsequently received rituximab, hyper-CVAD, and dasatinib, along with prophylactic intrathecal prophylactic chemotherapy. The patient achieved a prolonged remission but eventually relapsed, 4 years later. Had it not been for this fortuitous discovery, the patient would not have been treated with tyrosine kinase inhibitors. We emphasize that FISH and PCR testing for BCR-ABL1 rearrangement are integral to arriving at an accurate diagnosis and should be routinely tested on B-LBL biopsy specimens.

A new approach to arylhydrazides via the reaction of the Mitsunobu reagent with arynes: further application to access diverse nitrogen-containing heterocycles in one pot

2017 ◽  
Vol 4 (8) ◽  
pp. 1636-1639 ◽  
Author(s):  
Bin Cheng ◽  
Bian Bao ◽  
Yanhe Chen ◽  
Ning Wang ◽  
Yun Li ◽  
...  
Keyword(s):  
One Pot ◽  
New Approach ◽  
Mild Conditions ◽  
Highly Active ◽  
Active Intermediates

A new route to arylhydrazides involving the reaction of two highly active intermediates, the 1,3-zwitterion generated in situ from the Mitsunobu reagent and arynes, under mild conditions has been developed.

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