scholarly journals Automated Optical Counting of Blood Platelets

Blood ◽  
1971 ◽  
Vol 38 (4) ◽  
pp. 422-430 ◽  
Author(s):  
GEOFFREY M. BRITTIN ◽  
SHIRLEY A. DEW ◽  
ELVI K. FEWELL
Keyword(s):  
Whole Blood ◽  
Blood Platelets ◽  
Venous Blood ◽  
Human Platelets ◽  
Blood Samples ◽  
Platelet Counts ◽  
Large Numbers ◽  
Significant Difference ◽  
Electronic Method ◽  
Electronic Counting

Abstract We have evaluated the use of an optical particle counter to perform automated platelet counts on whole blood. The erythrocytes were lysed by dilution of whole blood with 2 M urea and the remaining platelets and leukocytes were enumerated by a darkfield microscope optical system that detects light diffracted by them. A suspension of fixed human platelets available commercially was highly satisfactory for standardization. The method gave accurate and reproducible platelet counts, comparable with those of electronic particle counting on venous blood and substantially more reliable platelet counts on thrombocytopenic and finger-puncture blood samples. We believe that errors resulting from the electronic method were caused by technical difficulties of sample handling and not to an intrinsic error in electronic counting. By using the automated optical method we found no significant difference between the platelet counts of capillary and venous blood, although capillary platelet counts had twice the variability of venous counts. The optical technique has important advantages over electronic platelet counting, and its superiority appears to be due to the ability to count platelets in diluted whole blood rather than in plasma. It should prove especially useful in performing the large numbers of platelet counts on thrombocytopenic and finger-puncture blood samples that are increasingly important for management of patients receiving chemotherapy.

scholarly journals Perbedaan Hitung Jumlah Trombosit Menggunakan Darah Vena dan Darah Kapiler

2016 ◽  
Vol 3 (2) ◽  
pp. 81
Author(s):  
Hieronymus Rayi Prasetya ◽  
Maria Irena Dentri ◽  
Sistiyono Sistiyono
Keyword(s):  
Platelet Count ◽  
Blood Platelets ◽  
Venous Blood ◽  
Blood Platelet ◽  
Capillary Blood ◽  
Blood Capillaries ◽  
Blood Samples ◽  
Platelet Counts ◽  
Significant Difference ◽  
Blood Platelet Count

Background: Platelets play a role in hemostasis which is the body's mechanisms to prevent and stop the bleeding. Platelets participate in the effort to close the wound, so that the body does not experience a loss of blood and protected from foreign cells. Examination of the platelet count is very important in the diagnosis of diseases, one of which is the diagnosis of dengue hemorrhagic fever (DHF). Examination of blood counts, especially platelets in clinical laboratories causes blood samples in use are not always the venous blood but could use capillary blood. Capillary blood samples are used primarily in pediatric patients, because the venous blood sampling is difficult, patient loads, and also shorten the time when taking blood. The purpose of this study was to determine whether there is a difference in counting the number of platelets using samples of blood veins and capillaries. Methods: Quantitative research with observational approach using a cross sectional study design in the 30 samples of student D3 Health Analyst STIKes To Nation Yogyakarta. Statistical methods in use are independent T test. Results: The research subjects were 30 samples of student D3 Health Analyst STIKes To Nation Yogyakarta. The results of the examination of venous blood platelet count and blood capillaries have different average values ​​are 247 530 cells / ml of blood, for blood platelets veins and 184 270 cells / ml of blood for capillary blood platelets. Spearman correlation analysis Obtained results of the examination of venous blood platelet count and blood capillaries normal distribution (p> 0.05). 0.129 venous blood platelet counts, while the number of blood platelets kapilernya 0.089. Conclusion: There is a significant difference from the results of counting the number of blood platelets using veins and capillaries, where the use of capillary blood samples showed that lower platelet counts.

scholarly journals Evaluation of a Technic for Counting Dog and Human Platelets

Blood ◽  
1965 ◽  
Vol 25 (2) ◽  
pp. 261-266 ◽  
Author(s):  
ROBERT I. WEED ◽  
S. LEE CRUMP ◽  
SCOTT N. SWISHER ◽  
Norma C. Trabold
Keyword(s):  
Human Blood ◽  
Whole Blood ◽  
Blood Platelets ◽  
Ammonium Oxalate ◽  
Human Platelets ◽  
Blood Samples ◽  
Platelet Counts ◽  
Statistical Errors ◽  
Human Blood Samples

Abstract 1. The addition of Na2EDTA (2.5 to 5 mg./2 ml. of whole blood) to dog or human blood samples has been shown to eliminate the problem of platelet clumping and to provide satisfactory samples for use in enumeration of blood platelets. The statistical errors of platelet counts in blood samples prepared with Na2EDTA agree well with previously published results obtained with unmodified technic. In blood samples prepared without Na2EDTA, where clumping is appreciable, the field error is inflated. 2. Variation in the time during which the platelets are permitted to settle in the chamber before counting, between limits of 30 and 90 minutes, has no effect on the count. 3. The period of standing of the diluted blood in 1 per cent ammonium oxalate, from 30 minutes to four hours, appears to have no effect on the out-come of the platelet counting results when the platelets are not clumped.

The Effect of Venous Occlusion on the Sequestration of Blood Platelets

1972 ◽  
Vol 28 (03) ◽  
pp. 383-392 ◽  
Author(s):  
J Hladovec ◽  
Z Koleilat ◽  
I Přerovský
Keyword(s):  
Blood Platelets ◽  
Venous Blood ◽  
Venous Occlusion ◽  
Blood Samples ◽  
Pronounced Decrease ◽  
Platelet Counts ◽  
Human Volunteers ◽  
Opposite Change

SummaryThe venous occlusion of all four legs in rats caused a highly significant decrease of platelet counts in venous blood especially after the correction for an opposite change in haematocrit. A very pronounced decrease in platelets was observed in human volunteers after a venostasis in one arm in the blood drawn from the occluded limb just before the release of occlusion. Similar decreases were found after a venostasis of both legs in postocclusion blood samples. The decrease in blood platelets results from temporary sequestration in the occluded limbs. The decreases of platelets after a 10 min occlusion of both legs are more pronounced in patients with post thrombotic states.

scholarly journals Analytical performance of lateral flow immunoassay for SARS-CoV-2 exposure screening on venous and capillary blood samples

2020 ◽  
Author(s):  
Margaret A Black ◽  
Guomiao Shen ◽  
Xiaojun Feng ◽  
Wilfredo Garcia Beltran ◽  
Yang Feng ◽  
...  
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Venous Blood ◽  
Capillary Blood ◽  
Lateral Flow ◽  
Antibody Detection ◽  
Lateral Flow Immunoassay ◽  
Blood Samples ◽  
Significant Difference ◽  
Finger Stick

Objectives: Numerous serologic immunoassays have been launched to detect antibodies to SARS-CoV-2, including rapid tests. Here, we validate use of a lateral flow immunoassay (LFI) intended for rapid screening and qualitative detection of anti-SARS-CoV-2 IgM and IgG in serum, plasma, and whole blood, and compare results with ELISA. We also seek to establish the value of LFI testing on blood obtained from a capillary blood sample. Methods: Samples collected by venous blood draw and capillary finger stick were obtained from patients with SARS-CoV-2 detected by RT-qPCR and control patients negative for SARS-CoV-2. Samples were tested with the 2019-nCoV IgG/IgM Detection Kit (Colloidal Gold) lateral flow immunoassay, and antibody calls were compared with results obtained by ELISA. Results: The Biolidics LFI kit shows clinical sensitivity of 92% at 7 days after PCR diagnosis of SARS-CoV-2 on venous blood. Test specificity was 92% for IgM and 100% for IgG. There was no significant difference in detecting IgM and IgG with Biolidics LFI and ELISA at D0 and D7 (p=1.00), except for detection of IgM at D7 (p=0.04). Finger stick whole blood of SARS-CoV-2 patients showed 93% sensitivity for antibody detection. Conclusions: Clinical performance of Biolidics 2019-nCoV IgG/IgM Detection Kit (Colloidal Gold) is comparable to ELISA and showed consistent results across different sample types. Furthermore, we show that capillary blood obtained by finger stick shows similar sensitivity for detecting anti-SARS-CoV-2 IgM and IgG antibodies as venous blood samples. This provides an opportunity for decentralized rapid testing in the community and may allow point-of-care and longitudinal self-testing for the presence of anti-SARS-CoV-2 antibodies.

Markers of Hemostatic Activation in Affected Coronary Arteries during Angioplasty

1994 ◽  
Vol 72 (05) ◽  
pp. 672-675 ◽  
Author(s):  
Nicolas W Shammas ◽  
Michael J Cunningham ◽  
Richard M Pomearntz ◽  
Charles W Francis
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Venous Blood ◽  
Balloon Inflation ◽  
Blood Samples ◽  
Hemostatic System ◽  
Significant Difference ◽  
Hemostatic Markers ◽  
Artery Disease ◽  
High Doses

SummaryTo characterize the extent of early activation of the hemostatic system following angioplasty, we obtained blood samples from the involved coronary artery of 11 stable angina patients during the procedure and measured sensitive markers of thrombin formation (fibrino-peptide A, prothrombin fragment 1.2, and soluble fibrin) and of platelet activation ((3-thromboglobulin). Levels of hemostatic markers in venous blood obtained from 14 young individuals with low pretest probability for coronary artery disease were not significantly different from levels in venous blood or intracoronary samples obtained prior to angioplasty. Also, there was no translesional (proximal and distal to the lesion) gradient in any of the hemostatic markers before or after angioplasty in samples obtained between 18 and 21 min from the onset of the first balloon inflation. Furthermore, no significant difference was noted between angioplasty and postangioplasty intracoronary concentrations. We conclude that intracoronary hemostatic activation does not occur in the majority of patients during and immediately following coronary angioplasty when high doses of heparin and aspirin are administered.

Carbon Dioxide Equilibration in Canine Lungs during Rebreathing and Acute Alkalosis

1979 ◽  
Vol 57 (5) ◽  
pp. 385-388 ◽  
Author(s):  
R. D. Latimer ◽  
G. Laszlo
Keyword(s):  
Whole Blood ◽  
Base Excess ◽  
Venous Blood ◽  
Main Pulmonary Artery ◽  
Mixed Venous Blood ◽  
Arterial Blood ◽  
Significant Difference ◽  
Venous Pco2 ◽  
Pulmonary Arterial ◽  
Gas Pressures

1. The left lower lobe of the lungs of six anaesthetized dogs were isolated by the introduction of a bronchial cannula at thoracotomy. Catheters were introduced into the main pulmonary artery and a vein draining the isolated lobe. 2. Blood-gas pressures and pH were measured across the isolated lobe and compared with gas pressures in alveolar samples from the lobe. 3. When the isolated lobe was allowed to reach gaseous equilibrium with pulmonary arterial blood for 30 min, there was no significant difference between alveolar and pulmonary venous Pco2. Mean values of whole-blood base excess were similar in pulmonary arterial and pulmonary venous blood. 4. After injection of 20 ml of 8·4% sodium bicarbonate solution into a peripheral vein, Pco2, pH and plasma bicarbonate concentrations rose in the mixed venous blood. There was no change of whole-blood base excess across the lung, indicating that HCO−3, as distinct from dissolved CO2, did not enter lung tissue in measurable amounts. 5. No systematic alveolar—pulmonary venous Pco2 differences were demonstrated in this preparation other than those explicable by maldistribution of lobar blood flow.

Effects of Different Methods Used to Take Blood Samples on Blood Glucose Measurements

2021 ◽  
pp. 105477382110247
Author(s):  
Eda Ergin ◽  
Ayten Zaybak
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance Test ◽  
Venous Blood ◽  
Tolerance Test ◽  
Capillary Blood ◽  
Oral Glucose ◽  
Blood Samples ◽  
Significant Difference ◽  
Measurement Results ◽  
Quasi Experimental

The purpose of this study is to compare whether or not there is a difference between venous and capillary blood samples in blood glucose measurements and investigate the effects of different aseptic methods used in skin cleaning before collecting blood samples on measurement results. This quasi-experimental study was conducted with 109 patients. The capillary first and second blood drop values taken from the patients after fasting and at 2 hours following 75 g oral glucose tolerance test (OGTT) and capillary and venous blood glucose values were compared. There was no significant difference between the median venous blood glucose value and the capillary second blood drop value taken after wiping the finger with alcohol. There was no significant difference between the first and second blood drop values of capillary blood glucose 2 hours after OGTT.

scholarly journals The Effect of Exercise on the Total Leukocyte, Absolute Neutrophil, Lymphocyte and Platelet Counts among Sudanese Football Players

2021 ◽  
Vol 11 (6-S) ◽  
pp. 26-32
Author(s):  
Nihad Elsadig Babiker ◽  
Raheeg Mubark Anwer
Keyword(s):  
Physiological Stress ◽  
Hematological Parameters ◽  
Venous Blood ◽  
Age Groups ◽  
The Body ◽  
Case Group ◽  
Football Players ◽  
Platelet Counts ◽  
Significant Difference ◽  
Age Variation

Background: Aerobic exercise induces physiological stress on the body and brings changes in hematological parameters. This study aimed to determine the effect of playing football as an exercise on the total white blood cell count, absolute Neutrophil, lymphocyte and Platelet counts among Sudanese football players. Material and method:  This was a descriptive case control study, done on a number of football players in Khartoum state, Sudan and conducted within the period from August 2021 to October 2021. Three ml of venous blood samples had been collected from every participant in the study, parameters had been measured and calculated using blood cells counter (Sysmex KX-21N).                                                           Results: When compared the TWBCs, Absolute Neutrophil, Lymphocytes and platelet counts between cases and control there was a significant differences with (P ≤0.05). Also there was a significant difference in the case group before and after one hour of exercise with (P =0.00), and in significant differences between different age groups with (P ≥0.05). Conclusion: Playing football for 1 hour cause a significant increase in the TWBCs, absolute Neutrophils, absolute Lymphocytes and Platelets counts. Age variation has no effect on the measured parameters Keywords: Football, TWBCs, Neutrophils, Lymphocytes and Platelets.

scholarly journals Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0256423
Author(s):  
William M. Dickerson ◽  
Rebecca Yu ◽  
Helena U. Westergren ◽  
Jonathan Paraskos ◽  
Philipp Schatz ◽  
...  
Keyword(s):  
Point Of Care ◽  
Venous Blood ◽  
Reference Method ◽  
Time Data ◽  
Platelet Recovery ◽  
Blood Samples ◽  
Impedance Analyzer ◽  
Platelet Counts ◽  
Platelet Antibodies ◽  
Care Assessment

Background Point-of-care (PoC) testing of platelet count (PLT) provides real-time data for rapid decision making. The goal of this study is to evaluate the accuracy and precision of platelet counting using a new microvolume (8 μL), absolute counting, 1.5 kg cytometry-based blood analyzer, the rHEALTH ONE (rHEALTH) in comparison with the International Society of Laboratory Hematology (ISLH) platelet method, which uses a cytometer and an impedance analyzer. Methods Inclusion eligibility were healthy adults (M/F) ages 18–80 for donation of fingerprick and venous blood samples. Samples were from a random N = 31 volunteers from a single U.S. site. Samples were serially diluted to test thrombocytopenic ranges. Interfering substances and conditions were tested, including RBC fragments, platelet fragments, cholesterol, triglycerides, lipids, anti-platelet antibodies, and temperature. Results The concordance between the rHEALTH and ISLH methods had a slope = 1.030 and R2 = 0.9684. The rHEALTH method showed a correlation between capillary and venous blood samples (slope = 0.9514 and R2 = 0.9684). Certain interferents changed platelet recovery: RBC fragments and anti-platelet antibodies with the ISLH method; platelet fragments and anti-platelet antibodies on the rHEALTH; and RBC fragments, platelets fragments, triglycerides and LDL on the clinical impedance analyzer. The rHEALTH’s precision ranged from 3.1–8.0%, and the ISLH from 1.0–10.5%. Conclusions The rHEALTH method provides similar results with the reference method and good correlation between adult capillary and venous blood samples. This demonstrates the ability of the rHEALTH to provide point-of-care assessment of normal and thrombocytopenic platelet counts from fingerprick blood with high precision and limited interferences.

scholarly journals Diadenosine 5',5'''-p1,p4-tetraphosphate deficiency in blood platelets of the Chediak-Higashi syndrome

Blood ◽  
1985 ◽  
Vol 66 (3) ◽  
pp. 735-737 ◽  
Author(s):  
BK Kim ◽  
FC Chao ◽  
R Leavitt ◽  
AS Fauci ◽  
KM Meyers ◽  
...  
Keyword(s):  
Whole Blood ◽  
Marked Decrease ◽  
Blood Platelets ◽  
Human Platelets ◽  
Dense Granule ◽  
Atp Level ◽  
Dense Granules ◽  
Moderate Reduction ◽  
Normal Human ◽  
Chediak Higashi Syndrome

Abstract Diadenosine tetraphosphate (AP4A) is an unusual nucleotide found in a variety of cells, including platelets. It has been suggested that platelet AP4A is stored in the dense granules and is metabolically inactive. We have studied the AP4A content of blood platelets in two patients and three cattle with Chediak-Higashi syndrome (CHS), a hereditary platelet defect with dense granule deficiency. Acid-soluble extractions of whole blood and platelets were neutralized. The adenosine triphosphate (ATP) level was measured by luminescence technique. To measure the AP4A content, the neutralized extract was treated with phosphomonoesterase for removal of ATP. The AP4A content was then measured by coupling the phosphodiesterase and luciferase reaction. The AP4A content was 0.43 nmol/mg protein for normal human platelets and 0.004 nmol/mg protein for CHS platelets. The ATP/AP4A ratio was 67 for normal and 3,023 for CHS platelets. The whole blood AP4A was reduced by 89% in CHS patients who had only a slight decrease in ATP level (26% reduction). Similarly, bovine platelets with CHS showed a marked decrease of AP4A content and a moderate reduction of the ATP level. The platelet ATP/AP4A ratio was 351 and 3,133 for normal and CHS cattle, respectively. Results demonstrate a marked reduction of AP4A in CHS platelets and suggest that AP4A may be a useful marker for the measurement of dense granule content in platelets.

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