scholarly journals Phagocytosis and intracellular killing by peripheral blood monocytes of patients with monocytic leukemia

Blood ◽  
1982 ◽  
Vol 59 (6) ◽  
pp. 1234-1238
Author(s):  
R van Furth ◽  
PC Leijh ◽  
TL van Zwet ◽  
MT van den Barselaar
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Monocytes ◽  
Intracellular Killing ◽  
Monocytic Leukemia ◽  
Interesting Phenomenon ◽  
Opsonic Activity ◽  
Peripheral Blood Monocytes ◽  
Serum Factors ◽  
Healthy Donors ◽  
The Mean

This article concerns a study on the endocytic functions of circulating monocytes from 12 patients with acute or chronic monocytic leukemia. The results show that phagocytosis and intracellular killing of Staphylococcus aureus are impaired in only two patients and that the opsonic activity of the serum of all patients is normal. With respect to the intracellular killing of ingested Staphylococcus aureus, an interesting phenomenon was found in that the cells of patients with monocytic leukemia proved to be in a state of activation, as shown by the finding that patients' monocytes with normal phagocytosis killed about 64% of the ingested bacteria in the absence of extracellular stimulation by serum factors. When extracellular serum was present, the mean killing index rose to only 69%. This is unlike the situation seen in monocytes from healthy donors, where no killing occurs in the absence of extracellular serum and extracellular stimulation by serum factors is mandatory for optimal intracellular killing.

scholarly journals Phagocytosis and intracellular killing by peripheral blood monocytes of patients with monocytic leukemia

Blood ◽  
1982 ◽  
Vol 59 (6) ◽  
pp. 1234-1238 ◽  
Author(s):  
R van Furth ◽  
PC Leijh ◽  
TL van Zwet ◽  
MT van den Barselaar
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Monocytes ◽  
Intracellular Killing ◽  
Monocytic Leukemia ◽  
Interesting Phenomenon ◽  
Opsonic Activity ◽  
Peripheral Blood Monocytes ◽  
Serum Factors ◽  
Healthy Donors ◽  
The Mean

Abstract This article concerns a study on the endocytic functions of circulating monocytes from 12 patients with acute or chronic monocytic leukemia. The results show that phagocytosis and intracellular killing of Staphylococcus aureus are impaired in only two patients and that the opsonic activity of the serum of all patients is normal. With respect to the intracellular killing of ingested Staphylococcus aureus, an interesting phenomenon was found in that the cells of patients with monocytic leukemia proved to be in a state of activation, as shown by the finding that patients' monocytes with normal phagocytosis killed about 64% of the ingested bacteria in the absence of extracellular stimulation by serum factors. When extracellular serum was present, the mean killing index rose to only 69%. This is unlike the situation seen in monocytes from healthy donors, where no killing occurs in the absence of extracellular serum and extracellular stimulation by serum factors is mandatory for optimal intracellular killing.

scholarly journals Cytochemical, functional, and proliferative characteristics of promonocytes and monocytes from patients with monocytic leukemia

Blood ◽  
1983 ◽  
Vol 62 (2) ◽  
pp. 298-304 ◽  
Author(s):  
R van Furth ◽  
TL van Zwet
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Predictive Value ◽  
Mononuclear Phagocytes ◽  
Acute Monocytic Leukemia ◽  
Blood Monocytes ◽  
Monocytic Leukemia ◽  
Peripheral Blood Monocytes ◽  
Normal Individuals ◽  
The Mean

Abstract This article deals with a prospective study on the cytochemical, functional, and proliferative characteristics of promonocytes and bone marrow and peripheral blood monocytes of 20 patients with acute monocytic leukemia and 7 patients with chronic monocytic leukemia. The results show a wide variation in the peroxidase and esterase activities in these cells, whereas the percentages of mononuclear phagocytes with Fc gamma and C3b receptors did not differ appreciably from those in normal individuals. A discriminant analysis of these data and corresponding data from normal individuals showed that a below-normal peroxidase activity of circulating monocytes has predictive value for the presence of monocytic leukemia; a below-normal esterase activity has less, but nevertheless some, predictive value in this respect. An increase in the percentage of circulating monocytes, a decrease in the percentage of Fc gamma or C3b receptors, and a decline in the ability to phagocytose bacteria has no predictive value for the presence of monocytic leukemia. The mean percentage of patients' promonocytes that incorporated 3H-thymidine amounted to 80.9%, which is close to the control value in normal individuals. The mean values for the labeling indices of cultured bone marrow and peripheral blood monocytes are 1.0% and 0.74%, respectively; when 3H-thymidine was added to whole blood, the labeling index of the monocytes amounted to 3.6%. These percentages are only a little higher than those found for monocytes of normal individuals. These results indicate that the majority of the circulating monocytes in acute and chronic monocytic leukemia are not actively dividing or blast cells.

scholarly journals Cytochemical, functional, and proliferative characteristics of promonocytes and monocytes from patients with monocytic leukemia

Blood ◽  
1983 ◽  
Vol 62 (2) ◽  
pp. 298-304
Author(s):  
R van Furth ◽  
TL van Zwet
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Predictive Value ◽  
Mononuclear Phagocytes ◽  
Acute Monocytic Leukemia ◽  
Blood Monocytes ◽  
Monocytic Leukemia ◽  
Peripheral Blood Monocytes ◽  
Normal Individuals ◽  
The Mean

This article deals with a prospective study on the cytochemical, functional, and proliferative characteristics of promonocytes and bone marrow and peripheral blood monocytes of 20 patients with acute monocytic leukemia and 7 patients with chronic monocytic leukemia. The results show a wide variation in the peroxidase and esterase activities in these cells, whereas the percentages of mononuclear phagocytes with Fc gamma and C3b receptors did not differ appreciably from those in normal individuals. A discriminant analysis of these data and corresponding data from normal individuals showed that a below-normal peroxidase activity of circulating monocytes has predictive value for the presence of monocytic leukemia; a below-normal esterase activity has less, but nevertheless some, predictive value in this respect. An increase in the percentage of circulating monocytes, a decrease in the percentage of Fc gamma or C3b receptors, and a decline in the ability to phagocytose bacteria has no predictive value for the presence of monocytic leukemia. The mean percentage of patients' promonocytes that incorporated 3H-thymidine amounted to 80.9%, which is close to the control value in normal individuals. The mean values for the labeling indices of cultured bone marrow and peripheral blood monocytes are 1.0% and 0.74%, respectively; when 3H-thymidine was added to whole blood, the labeling index of the monocytes amounted to 3.6%. These percentages are only a little higher than those found for monocytes of normal individuals. These results indicate that the majority of the circulating monocytes in acute and chronic monocytic leukemia are not actively dividing or blast cells.

Is the Success of Cefazolin plus Ertapenem in Methicillin-Susceptible Staphylococcus aureus Bacteremia Based on Release of Interleukin 1-beta?

2022 ◽  
Author(s):  
Dan Smelter ◽  
Mary Hayney ◽  
George Sakoulas ◽  
Warren Rose
Keyword(s):  
Staphylococcus Aureus ◽  
Peripheral Blood ◽  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Blood Monocytes ◽  
Salvage Regimen ◽  
Peripheral Blood Monocytes ◽  
Staphylococcus Aureus Bacteremia

Cefazolin and ertapenem has been shown to be an effective salvage regimen for refractory methicillin-susceptible Staphylococcus aureus bacteremia. Our findings suggest cefazolin plus ertapenem in vitro stimulates interleukin-1β release from peripheral blood monocytes both with and without S. aureus presence. This IL-1β augmentation was primarily driven by ertapenem. These findings support further exploration of cefazolin plus ertapenem in MSSA bacteremia and may partially explain its marked potency in vivo despite modest synergy in vitro .

Investigate the role of GPR15/BOB in the SLE patients

2018 ◽  
Vol 6 (1) ◽  
pp. 19-32
Author(s):  
Caroline G. Jackson ◽  
Donald R. Kwan
Keyword(s):  
Peripheral Blood ◽  
Messenger Rna ◽  
Viral Envelope ◽  
Orphan Receptor ◽  
Blood Monocytes ◽  
Rt Pcr ◽  
Peripheral Blood Monocytes ◽  
Healthy Donors ◽  
Hiv 1

GPR15 functions as a cellular co-receptor for some isolates of HIV-1, HIV-2, and SIV through interactions with several viral envelope proteins. The objective of this study was to investigate the expression of orphan receptor GPR15/BOB in the serum of SLE patients and non-SLE healthy people. GPR15/BOB expression was analysed by flow cytometry while, GPR15/BOB messenger RNA was examined in peripheral blood monocytes by RT-PCR. GPR15/BOB mRNA was detected in all periphral blood of SLE patients examined. Further, a significant increase in GPR15/BOB expression as measured by mean fluorescence intensity was observed on SLE PB neutrophils compared to these cell populations from healthy donors. We concluded that GPR15/BOB is expressed in monocytes and neutrophils in peripheral blood, and expression is up-regulated in SLE patients compared to controls. GPR15/BOB may play a role in SLE pathogenesis.

scholarly journals Expression of c-fos, c-myb, and c-myc in human monocytes: correlation with monocytic differentiation

Blood ◽  
1987 ◽  
Vol 69 (5) ◽  
pp. 1542-1545
Author(s):  
J Lee ◽  
K Mehta ◽  
MB Blick ◽  
JU Gutterman ◽  
G Lopez-Berestein
Keyword(s):  
Human Peripheral Blood ◽  
Terminal Differentiation ◽  
Functional Differentiation ◽  
Leukemia Cells ◽  
Blood Monocytes ◽  
Monocytic Differentiation ◽  
Monocytic Leukemia ◽  
Peripheral Blood Monocytes ◽  
Human Monocytic Leukemia ◽  
Monocytic Lineage

Terminal differentiation of human monocytic leukemia cells (THP-1 cells) was associated with the induction of c-fos, the down regulation of c-myb, and no significant change in the level of c-myc expression. Gamma interferon, which resulted in a slight decrease in c-myb but no change in c-fos or c-myc expression, had a transient antiproliferative effect without a morphological or functional differentiation of THP-1 cells. Resting human peripheral blood monocytes have a high c-fos, a low c-myc, and no detectable c-myb expression. These findings suggest that a switch in c-fos/c-myb expression is associated with the terminal differentiation of cells of the monocytic lineage.

scholarly journals Staphylococcus aureus as a target of peripheral blood microbicidal factors from healthy donors

2021 ◽  
Vol 38 (2) ◽  
pp. 6-13
Author(s):  
Anatoliy P. Godovalov ◽  
Iosif A. Boev
Keyword(s):  
Staphylococcus Aureus ◽  
Hydroxyl Radicals ◽  
Immunoglobulin G ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Clinical Manifestations ◽  
Blood Monocytes ◽  
Complement Components ◽  
Bacteriolytic Activity ◽  
Healthy Donors

Objective. The aim of investigation was to evaluate the bacteriolytic activity of the peripheral blood of healthy donors against S. aureus. Currently, much attention is paid to the involvement of opportunistic microorganisms in the development of infectious and inflammatory diseases, among which one of the leading places is occupied by the processes of staphylococcal etiology. Staphylococcus aureus possesses a unique spectrum of pathogenic factors, which, together with intracellular persistence, allow staphylococci to avoid exposure to immune factors and other agents. Materials and methods. The bacteriolytic activity of the peripheral blood of 32 healthy donors, as well as the ability of the whole blood and serum to destroy biofilms, were evaluated. The phagocytic activity of peripheral blood monocytes and neutrophils and the ability to produce hydroxyl radicals were analyzed. For opsonization of S. aureus, a commercial immunoglobulin G or donor serum was used. Results. It was shown that the whole peripheral blood practically does not have a significant effect on the number of viable cells of S. aureus. However, freshly obtained blood serum significantly destroys the biofilm. It has been established that a fifth part of peripheral blood leukocytes is absorbed by S. aureus. After opsonization of microbial cells with immunoglobulin G, the indices of phagocytic activity of monocytes and neutrophils did not change significantly. When using freshly obtained serum for opsonization of objects, a stimulating effect on the production of hydroxyl radicals by leukocytes was revealed (2758.7 725.3 and 870.6 197.4 related light units, respectively; p 0.05). After heating the serum at 56 C, the stimulating effect was leveled (1091.1 234.7 related light units; p 0.05 for samples with non-opsonized objects). In general, the obtained data indicate that the complement components can be recognized as the most effective system for the elimination of S. aureus. Conclusions. Thus, S. aureus uniquely adapted to the human body that allows staphylococci to persist for a long time without clinical manifestations. It can be assumed that among the factors of the immune system, the proteins of the complement system, which destroy both S. aureus cells and the biofilm matrix, probably have the most effective bactericidal action. However, the effectiveness of this system depends on the protein-synthesizing function of the liver, the availability of microorganisms to the action of complement.

scholarly journals Expression of c-fos, c-myb, and c-myc in human monocytes: correlation with monocytic differentiation

Blood ◽  
1987 ◽  
Vol 69 (5) ◽  
pp. 1542-1545 ◽  
Author(s):  
J Lee ◽  
K Mehta ◽  
MB Blick ◽  
JU Gutterman ◽  
G Lopez-Berestein
Keyword(s):  
Human Peripheral Blood ◽  
Terminal Differentiation ◽  
Functional Differentiation ◽  
Leukemia Cells ◽  
Blood Monocytes ◽  
Monocytic Differentiation ◽  
Monocytic Leukemia ◽  
Peripheral Blood Monocytes ◽  
Human Monocytic Leukemia ◽  
Monocytic Lineage

Abstract Terminal differentiation of human monocytic leukemia cells (THP-1 cells) was associated with the induction of c-fos, the down regulation of c-myb, and no significant change in the level of c-myc expression. Gamma interferon, which resulted in a slight decrease in c-myb but no change in c-fos or c-myc expression, had a transient antiproliferative effect without a morphological or functional differentiation of THP-1 cells. Resting human peripheral blood monocytes have a high c-fos, a low c-myc, and no detectable c-myb expression. These findings suggest that a switch in c-fos/c-myb expression is associated with the terminal differentiation of cells of the monocytic lineage.

scholarly journals The Effect of Bacteriophage Preparations on Intracellular Killing of Bacteria by Phagocytes

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Ewa Jończyk-Matysiak ◽  
Marzanna Łusiak-Szelachowska ◽  
Marlena Kłak ◽  
Barbara Bubak ◽  
Ryszard Międzybrodzki ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Phage Therapy ◽  
Practical Importance ◽  
Polymorphonuclear Neutrophils ◽  
Blood Monocytes ◽  
Intracellular Killing ◽  
Chronic Infections ◽  
Peripheral Blood Monocytes ◽  
Gram Negative ◽  
Bactericidal Properties

Intracellular killing of bacteria is one of the fundamental mechanisms against invading pathogens. Impaired intracellular killing of bacteria by phagocytes may be the reason of chronic infections and may be caused by antibiotics or substances that can be produced by some bacteria. Therefore, it was of great practical importance to examine whether phage preparations may influence the process of phagocyte intracellular killing of bacteria. It may be important especially in the case of patients qualified for experimental phage therapy (approximately half of the patients with chronic bacterial infections have their immunity impaired). Our analysis included 51 patients with chronic Gram-negative and Gram-positive bacterial infections treated with phage preparations at the Phage Therapy Unit in Wroclaw. The aim of the study was to investigate the effect of experimental phage therapy on intracellular killing of bacteria by patients’ peripheral blood monocytes and polymorphonuclear neutrophils. We observed that phage therapy does not reduce patients’ phagocytes’ ability to kill bacteria, and it does not affect the activity of phagocytes in patients with initially reduced ability to kill bacteria intracellularly. Our results suggest that experimental phage therapy has no significant adverse effects on the bactericidal properties of phagocytes, which confirms the safety of the therapy.

Sign in / Sign up

Export Citation Format

Share Document