scholarly journals Tuftsin induces tissue factor-like activity in human mononuclear cells and in monocytic cell lines

Blood ◽  
1990 ◽  
Vol 76 (4) ◽  
pp. 814-819 ◽  
Author(s):  
A Kornberg ◽  
R Catane ◽  
S Peller ◽  
S Kaufman ◽  
M Fridkin
Keyword(s):  
Tissue Factor ◽  
Cell Lines ◽  
Mononuclear Cells ◽  
Monocytic Cell ◽  
Human Mononuclear Cells ◽  
Monocytes And Macrophages ◽  
Normal Human ◽  
Natural Stimulator ◽  
Coagulation Pathway ◽  
Lymphoid Cell Lines

Abstract Normal human monocytes and macrophages generate potent procoagulant activity (PCA) resembling tissue factor (TF) in response to various stimuli. In this study we show that tuftsin, a natural stimulator of many functions of monocytes and macrophages, also stimulates a potent PCA in mixed mononuclear cells and monocytes, and a mild PCA in lymphocytes and cell lines of monocytic origin (U937 and THP). No activity was generated by several lymphoid cell lines and HL-60 cells. The PCA resembled TF in that it accelerated clotting through the extrinsic coagulation pathway and was inhibited by concanavalin-A and by monoclonal anti-TF antibodies. The induction of TF-like activity by tuftsin was dose- and time-dependent. It was located in the cell membrane and did not require T cells for expression. Generation of TF- like activity was prevented by actinomycin D, while cytarabine had no effect on this process, suggesting that expression of the activity depends on protein synthesis. Studies with various tuftsin analogs suggest that tuftsin stimulates generation of TF-like activity, as well as other functions of monocytes via the same receptors. The results with the monocytic cell lines show that tuftsin affects mainly mature cells. The induction of TF-like activity in mononuclear cells by tuftsin constitutes an important link between mononuclear cells and the immune and coagulation systems. It may play a major role in the pathogenesis of thromboembolism and fibrin deposition in various inflammatory and immunologic disorders.

scholarly journals Tuftsin induces tissue factor-like activity in human mononuclear cells and in monocytic cell lines

Blood ◽  
1990 ◽  
Vol 76 (4) ◽  
pp. 814-819
Author(s):  
A Kornberg ◽  
R Catane ◽  
S Peller ◽  
S Kaufman ◽  
M Fridkin
Keyword(s):  
Tissue Factor ◽  
Cell Lines ◽  
Mononuclear Cells ◽  
Monocytic Cell ◽  
Human Mononuclear Cells ◽  
Monocytes And Macrophages ◽  
Normal Human ◽  
Natural Stimulator ◽  
Coagulation Pathway ◽  
Lymphoid Cell Lines

Normal human monocytes and macrophages generate potent procoagulant activity (PCA) resembling tissue factor (TF) in response to various stimuli. In this study we show that tuftsin, a natural stimulator of many functions of monocytes and macrophages, also stimulates a potent PCA in mixed mononuclear cells and monocytes, and a mild PCA in lymphocytes and cell lines of monocytic origin (U937 and THP). No activity was generated by several lymphoid cell lines and HL-60 cells. The PCA resembled TF in that it accelerated clotting through the extrinsic coagulation pathway and was inhibited by concanavalin-A and by monoclonal anti-TF antibodies. The induction of TF-like activity by tuftsin was dose- and time-dependent. It was located in the cell membrane and did not require T cells for expression. Generation of TF- like activity was prevented by actinomycin D, while cytarabine had no effect on this process, suggesting that expression of the activity depends on protein synthesis. Studies with various tuftsin analogs suggest that tuftsin stimulates generation of TF-like activity, as well as other functions of monocytes via the same receptors. The results with the monocytic cell lines show that tuftsin affects mainly mature cells. The induction of TF-like activity in mononuclear cells by tuftsin constitutes an important link between mononuclear cells and the immune and coagulation systems. It may play a major role in the pathogenesis of thromboembolism and fibrin deposition in various inflammatory and immunologic disorders.

scholarly journals The heparin binding PECAM-1 adhesion molecule is expressed by CD34+ hematopoietic precursor cells with early myeloid and B-lymphoid cell phenotypes

Blood ◽  
1993 ◽  
Vol 82 (9) ◽  
pp. 2649-2663 ◽  
Author(s):  
SM Watt ◽  
J Williamson ◽  
H Genevier ◽  
J Fawcett ◽  
DL Simmons ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Line ◽  
Cell Lines ◽  
Adhesion Molecule ◽  
Progenitor Cell ◽  
Precursor Cell ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Normal Human ◽  
B Lymphoid

The platelet-endothelial cell adhesion molecule-1 (PE-CAM-1), defined by the CD31 monoclonal antibody (MoAb), was initially described as a cell-cell adhesion molecule mediating both homotypic and heterotypic adhesion. In this report, we show that enriched CD34+ human hematopoietic progenitor cell populations, containing early myeloid, erythroid, and multipotential progenitor cells, are CD31+. Analyses of CD34+ cell lines representing early myeloid, multipotential, and pre- pre-B-lymphoid progenitors indicate that precursors of both myeloid and B-lymphoid cells express PECAM-1 at high levels. Three-color flow- cytometric analyses also show that normal human bone marrow CD31+ CD34+ subsets coexpress myeloid (CD33) or B-lymphoid (CD19, CD10) markers. Except for the monocytic cell line, U937, all CD34- cell lines tested, which represent more mature stages of the myeloid, erythroid, and lymphoid lineages, expressed substantially lower or negligible levels of PECAM-1. Western blotting studies indicated that the CD31 MoAb, JC/70A, detected molecules in the 120- to 140-kD molecular weight range on the monocytic CD34- CD33+ CD31+ cell line, U937; on the CD34+ CD31+ CD33+ CD19- multipotential/lymphomyeloid precursor cell lines, KG1 and KG1B; on the CD34+ CD31+ CD19+ CD10+ CD33- precursor pre-pre-B-cell line, MIK-ALL; and on a CD34(+)-enriched precursor cell population from normal human bone marrow. A single molecular weight species was generally observed with enriched membrane preparations, whereas two PECAM-1 molecules were present in whole-cell lysates of cell lines and the CD34+ bone marrow cell subset. Preliminary studies show that a proportion of the PECAM-1 molecules on the lymphomyeloid/multipotential progenitor cell line, KG1, and on the monocytic cell line, U937, binds to heparin-sepharose. A soluble form of PECAM-1 also binds heparin- sepharose. The high level of expression of PECAM-1 on CD34+ cells suggests that this glycoprotein may function as a heterotypic adhesion molecule, possibly mediating multipotential, myeloid, and early-B- lymphoid precursor cell interactions with stromal cells and extracellular matrix molecules via heparan sulfate proteoglycans. It may also act as a homotypic adhesion molecule by interacting with PECAM- 1 on bone marrow stromal macrophage-like cells and endothelial cells or on endothelial cells during stem/progenitor cell migration. Thus, this molecule has the potential importance of directing both lineage commitment and trafficking of early hematopoietic progenitor cells.

scholarly journals Heterogeneity of B cell involvement in acute nonlymphocytic leukemia

Blood ◽  
1985 ◽  
Vol 66 (2) ◽  
pp. 342-344 ◽  
Author(s):  
AM Ferraris ◽  
WH Raskind ◽  
BH Bjornson ◽  
RJ Jacobson ◽  
JW Singer ◽  
...  
Keyword(s):  
Stem Cell ◽  
B Cell ◽  
Progenitor Cells ◽  
Cell Lines ◽  
Lymphoid Cell ◽  
Mononuclear Cells ◽  
Acute Nonlymphocytic Leukemia ◽  
Peripheral Blood Mononuclear ◽  
B Lymphoid ◽  
Lymphoid Cell Lines

Abstract In order to study the pattern of B cell involvement in acute nonlymphocytic leukemia (ANLL), multiple B lymphoid cell lines were established by Epstein-Barr virus transformation of peripheral blood mononuclear cells from two patients with the disease who were heterozygous for the X chromosome-linked glucose-6-phosphate dehydrogenase (G6PD). In one patient, the progenitor cells involved by the leukemia exhibited multipotent differentiative expression, whereas in the other patient the cells showed differentiative expression restricted to the granulocytic pathway. In the patient whose abnormal clone showed multipotent expression, the ratio of B-A G6PD in B lymphoid cell lines was skewed in the direction of type B (the enzyme characteristic of the leukemia clone) and significantly different from the 1:1 ratio expected. It is, therefore, likely that the neoplastic event occurred in a stem cell common to the lymphoid series as well as to the myeloid series. In contrast, evidence for B cell involvement was not detected in the patient whose ANLL progenitor cells exhibited restricted differentiative expression. These findings underscore the heterogeneity of ANLL. Clinically and morphologically similar malignancies in these two patients originated in progenitors with different patterns of stem cell differentiative expression. This difference may reflect differences in cause and pathogenesis.

scholarly journals Human malignant and mitogen-transformed cells contain retroviral P15E-related antigen.

1984 ◽  
Vol 159 (3) ◽  
pp. 964-969 ◽  
Author(s):  
G J Cianciolo ◽  
D Phipps ◽  
R Snyderman
Keyword(s):  
Cell Lines ◽  
Mononuclear Cells ◽  
Human Tumor ◽  
Malignant Cell ◽  
Transformed Cells ◽  
Blast Transformation ◽  
Structural Protein ◽  
Human Tumor Cells ◽  
Human Mononuclear Cells ◽  
Protein P15e

Virus-related oncogenes have been demonstrated in human tumor cells and may play a role in neoplastic transformation. Cancerous effusions contain inhibitors of monocyte function and are absorbed by monoclonal antibodies to the immunosuppressive retroviral structural protein, P15E. We therefore examined eight human malignant cell lines for P15E-related antigens, by indirect immunofluorescence. Up to 87% of fixed malignant cells were reactive with two different monoclonal anti-P15E antibodies, while under identical conditions approximately 7% of freshly isolated human mononuclear cells were positive. Differentiation of two tumor cell lines with dibutyryl cyclic AMP resulted in decreased anti-P15E reactivity. Blast transformation of human mononuclear cells with mitogens induced reactivity with anti-P15E. Thus human malignant and blast-transformed cells contain antigens related to P15E. Expression of this viral-related gene may occur during rapid cell division and be abnormally regulated in cancer cells, thus rendering them more resistant to immune destruction.

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