scholarly journals The ontogeny of key endoplasmic reticulum proteins in human embryonic and fetal red blood cells

Blood ◽  
1996 ◽  
Vol 87 (2) ◽  
pp. 762-770 ◽  
Author(s):  
R Hume ◽  
A Burchell ◽  
BB Allan ◽  
CR Wolf ◽  
RW Kelly ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Cytochrome P450 ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Glucose Production ◽  
Uridine Diphosphate ◽  
Protein Components ◽  
Cytochrome P450 Isozymes ◽  
Endoplasmic Reticulum Proteins

Recently, using immunohistochemical methods, we surprisingly found that endoplasmic reticulum glucose-6-phosphatase is present in human embryonic and fetal red blood cells (RBCs) but not in adult RBCs. The fact that an endoplasmic reticulum enzyme, whose major site of expression in adults is the liver, is present in human embryonic and fetal RBCs, particularly nucleated cells, indicated that it would be sensible to determine whether these cells also contain other endoplasmic reticulum enzyme systems normally found in adult liver. Therefore, we have studied the expression of other endoplasmic reticulum proteins and found that human embryonic and fetal RBC precursors contain other protein components of the glucose-6- phosphatase system, ie, the phosphate and glucose transport proteins as well as other enzymes (eg, uridine diphosphate- glucuronosyltransferases, cytochrome P450 isozymes, nicotinamide adenine dinucleotide phosphate cytochrome P450 oxidoreductase, and prostaglandin H synthase). In addition, we also found the predominantly cytosolic markers 15-hydroxyprostaglandin dehydrogenase, prostaglandins PGE2 and 13,14-dihydro-15-keto-PGE2. The expression of key enzymes that control glucose production, detoxification of endobiotics and xenobiotics, and the regulation of prostaglandin levels in embryonic and early fetal RBCs means that these cells may have an important role in protecting the developing conceptus before it establishes an efficient circulation and before all tissues fully express their normal complement of these enzymes.

scholarly journals УЛЬТРАМІКРОСКОПІЧНІ ПРОЯВИ У СТРУКТУРІ ПІДНИЖНЬОЩЕЛЕПНОЇ СЛИННОЇ ЗАЛОЗИ НАПРИКІНЦІ 2-ГО ТИЖНЯ ЕКСПЕРИМЕНТАЛЬНОГО ОПІОЇДНОГО ВПЛИВУ

World Science ◽  
2020 ◽  
Vol 1 (4(56)) ◽  
pp. 4-7
Author(s):  
Mykhalevych Marta
Keyword(s):  
Endothelial Cells ◽  
Endoplasmic Reticulum ◽  
Salivary Gland ◽  
Connective Tissue ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Acinar Cells ◽  
Ultrastructural Changes ◽  
Submandibular Salivary Gland ◽  
Perivascular Edema

This publication demonstrates the ultrastructural changes of the submandibular salivary gland under the opioid effect at the 2nd week of the experimental research.After 14 days of experimental opioid effect, we found the destructive changes in the organelles of exocrinocytes of acinar cells, as well as dyscirculatory processes. Capillary lumen was enlarged, overflowing with red blood cells. In the endothelium of the capillaries also noted the expansion of the granular endoplasmic reticulum. Small vacuoles appeared in the cytoplasm of endothelial cells. Single mitochondria located in the area of the endothelial cytoplasmic organelles are swollen. Due to the development of perivascular edema, the underlying connective tissue substance around the capillaries was impregnated with electron-illuminated transudate masses.

scholarly journals Platelet Purinergic Receptors in Thrombosis and Inflammation

2020 ◽  
Vol 40 (02) ◽  
pp. 145-152 ◽  
Author(s):  
Christian Gachet ◽  
Beatrice Hechler
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Adenosine Diphosphate ◽  
Specific Role ◽  
Uridine Diphosphate ◽  
Dense Granules ◽  
Acute Responses ◽  
Inflammatory Processes ◽  
Diphosphate Glucose ◽  
G Protein Coupled

AbstractIt took approximately 40 years from the seminal identification of adenosine diphosphate (ADP) as the factor R, an agent derived from red blood cells inducing platelet adhesion to glass, to the completion of the repertoire of its receptors on platelets and its importance in haemostasis and thrombosis. ADP, either derived from red blood cells or released by platelets themselves, stimulates platelets via two G protein-coupled receptors, P2Y1 and P2Y12. In addition, adenosine triphosphate, also contained in the platelet dense granules, activates the P2X1 cation channel. Each of these receptors plays a specific role during platelet activation and aggregation, with relevance to haemostasis, thrombosis and various inflammatory processes where platelets are involved including chronic responses such as atherosclerosis or acute responses such as sepsis, endotoxaemia or allergic asthma. Finally, platelets also express P2Y14, a receptor activated by released uridine diphosphate glucose. Although devoid of any known role in haemostasis, this receptor seems to play a specific role in neutrophil chemotaxis.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

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