Metabolomic fingerprinting of serum samples based on direct infusion mass spectrometry for lung cancer diagnosis

The current high mortality of human lung cancer stems largely from the lack of feasible, early disease detection tools. An effective test with serum metabolomics predictive models able to suggest patients harboring disease could expedite triage patient to specialized imaging assessment. Here, using a training-validation-testing-cohort design, we establish our high-resolution magic angle spinning (HRMAS) magnetic resonance spectroscopy (MRS)-based metabolomics predictive models to indicate lung cancer presence and patient survival using serum samples collected prior to their disease diagnoses. Studied serum samples were collected from 79 patients before (within 5.0 y) and at lung cancer diagnosis. Disease predictive models were established by comparing serum metabolomic patterns between our training cohorts: patients with lung cancer at time of diagnosis, and matched healthy controls. These predictive models were then applied to evaluate serum samples of our validation and testing cohorts, all collected from patients before their lung cancer diagnosis. Our study found that the predictive model yielded values for prior-to-detection serum samples to be intermediate between values for patients at time of diagnosis and for healthy controls; these intermediate values significantly differed from both groups, with an F1 score = 0.628 for cancer prediction. Furthermore, values from metabolomics predictive model measured from prior-to-diagnosis sera could significantly predict 5-y survival for patients with localized disease.

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Tandem mass spectrometry-based newborn screening strategy could be used to facilitate rapid and sensitive lung cancer diagnosis

OncoTargets and Therapy ◽

10.2147/ott.s99099 ◽

2016 ◽

pp. 2479 ◽

Cited By ~ 1

Author(s):

Yunfeng Cao ◽

Peng Gao ◽

ting huang ◽

Jun Dong ◽

Xiaoyu Sun ◽

...

Keyword(s):

Mass Spectrometry ◽

Lung Cancer ◽

Tandem Mass Spectrometry ◽

Newborn Screening ◽

Cancer Diagnosis ◽

Screening Strategy ◽

Tandem Mass ◽

Lung Cancer Diagnosis

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PSV-B-17 Additional dietary choline increases serum metabolites as determined by direct infusion mass spectrometry in post-gonadectomy kittens

Journal of Animal Science ◽

10.1093/jas/skab235.616 ◽

2021 ◽

Vol 99 (Supplement_3) ◽

pp. 335-336

Author(s):

Hannah Godfrey ◽

Alexandra Rankovic ◽

Caitlin E Grant ◽

Anna-Kate Shoveller ◽

Sarah Abood ◽

...

Keyword(s):

Mass Spectrometry ◽

Repeated Measure ◽

Acid Oxidation ◽

Healthy Weight ◽

Serum Concentrations ◽

Direct Infusion ◽

Serum Samples ◽

Intact Male ◽

Serum Metabolites ◽

Direct Infusion Mass Spectrometry

Abstract This study aimed to investigate changes in serum metabolites using direct infusion mass spectrometry (Di-MS) after 12 weeks with or without additional choline in growing, post-gonadectomy kittens. The present study was approved by the University of Guelph Animal Care Committee (AUP#4118). Intact, male kittens (3-months old; n = 15) were fed a base-diet to growth requirements (3310mg choline/kg DM) over an 11-week acclimation. Kittens were gonadectomized (week 1) and assigned to base-diet (n = 7) or base-diet with 300mg/kgBW0.75 additional choline (n = 8) and fed to mimic ad libitum food intake (3 meals totaling thrice daily requirements) for 12-weeks. Fasted serum samples were obtained and analyzed for 130 metabolites via Di-MS (week 0 and 12). The proc GLIMMIX procedure (SAS) was used to analyze differences between groups as a repeated measure. Additional choline resulted in higher serum concentrations of one-carbon metabolites such as methionine, serine, betaine, and sarcosine (P < 0.05). As expected, serum concentrations of phosphatidylcholines increased with additional choline, as did serum concentrations of lysophosphatidylcholines and sphingomyelins, compared to no additional choline (P < 0.05). There was minimal impact on acyl carnitines with a decrease in medium length acyl carnitines (P < 0.05). Additionally, there was an increase in serum concentrations of spermine, trans hydroxyproline, methionine sulfoxide, proline, and indole acetic acid, and a decrease in serum trimethylamine N-Oxide compared to control (P < 0.05). Effects of growth stage or gonadectomy may have resulted in increased serum amino acid concentrations such as asparagine, glutamine, glutamic acid, histidine, lysine, methionine, threonine, and tyrosine in both groups (P < 0.05). These results may indicate benefits for providing additional choline to post-gonadectomy kittens on one-carbon metabolism and fatty acid oxidation which could promote healthy weight and body condition. However, more research is warranted to further understand choline’s role in energy balance and its potential in prevention and treatment of feline obesity.

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