Sensitivity and specificity of loop-mediated isothermal amplification assay for diagnosis of extra-pulmonary tuberculosis: A cross-sectional study

INTRODUCTION: Extrapulmonary Tuberculosis (EPTB) accounts for 15- 25% of all TB cases. It is more difficult to diagnose than Pulmonary tuberculosis and often requires invasive procedures to obtain tissue and or fluid samples. Histology is time-consuming and establishing a diagnosis of TB with high specificity remains difficult. Tissue smear microscopy after special staining is often negative. Tissue culture often leads to considerable delays compromising patient care and outcomes. AIMS AND OBJECTIVES:1. To diagnose Extra Pulmonary Tuberculosis by Gene Xpert(Xpert MTB/Rif assay or CBNAAT) and Liquid Cultures. 2. To evaluate the Sensitivity and Specificity of Gene Xpert in Extra Pulmonary Tuberculosis in comparison with Liquid Culture MGIT960 system. MATERIALS AND METHODS: This retrospective cross-sectional study was carried out by reviewing all suspected extra pulmonary tuberculosis samples of 430 patients attending OPD at Institute of Respiratory Diseases, Jaipur from April 2020 to March 2021.The extrapulmonary samples (pleural fluid,CSF,pus,BAL,Ascitic fluid,Synovial fluid,Gastric aspirate,Liver aspirate) were subjected to GeneXpert and Liquid culture MGIT960 system. RESULTS: Of the 430 Extra Pulmonary Samples, The Sensitivity and Specificity of CBNAAT was 79.77% and 95.30% respectively in comparison with Liquid Culture. Out of the 430 Samples CBNAAT was Positive in 87 samples of which 71(81.60%) were Rifampicin sensitive and 16(18.39%) were Rifampicin Resistant.Out of the 430 Samples,Liquid cultures was Positive in 89 samples. CONCLUSION: Gene Xpert has a notable advantage of detecting tuberculosis within two hours which is acceptable to all clinicians to institute early treatment.CBNAAT is one of the rapid diagnostic tests available in the country and it should be routinely used under the public and private health sector effectively to detect early tuberculosis in Extra Pulmonary Samples.

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Evaluation of the performance of clinical predictors in estimating the probability of pulmonary tuberculosis among smear-negative cases in Northern Ethiopia: a cross-sectional study

BMJ Open ◽

10.1136/bmjopen-2020-037913 ◽

2020 ◽

Vol 10 (11) ◽

pp. e037913

Author(s):

Mala George ◽

Geert-Jan Dinant ◽

Efrem Kentiba ◽

Teklu Teshome ◽

Abinet Teshome ◽

...

Keyword(s):

Pulmonary Tuberculosis ◽

Cross Sectional Study ◽

Clinical Suspicion ◽

Secondary Outcome ◽

Northern Ethiopia ◽

Sectional Study ◽

Cross Sectional ◽

Smear Negative ◽

Culture Positive ◽

Culture Negative

ObjectivesTo evaluate the performance of the predictors in estimating the probability of pulmonary tuberculosis (PTB) when all versus only significant variables are combined into a decision model (1) among all clinical suspects and (2) among smear-negative cases based on the results of culture tests.DesignA cross-sectional study.SettingTwo public referral hospitals in Tigray, Ethiopia.ParticipantsA total of 426 consecutive adult patients admitted to the hospitals with clinical suspicion of PTB were screened by sputum smear microscopy and chest radiograph (chest X-ray (CXR)) in accordance with the Ethiopian guidelines of the National Tuberculosis and Leprosy Program. Discontinuation of antituberculosis therapy in the past 3 months, unproductive cough, HIV positivity and unwillingness to give written informed consent were the basis of exclusion from the study.Primary and secondary outcome measuresA total of 354 patients were included in the final analysis, while 72 patients were excluded because culture tests were not done.ResultsThe strongest predictive variables of culture-positive PTB among patients with clinical suspicion were a positive smear test (OR 172; 95% CI 23.23 to 1273.54) and having CXR lesions compatible with PTB (OR 10.401; 95% CI 5.862 to 18.454). The regression model had a good predictive performance for identifying culture-positive PTB among patients with clinical suspicion (area under the curve (AUC) 0.84), but it was rather poor in patients with a negative smear result (AUC 0.64). Combining all the predictors in the model compared with only the independent significant variables did not really improve its performance to identify culture-positive (AUC 0.84–0.87) and culture-negative (AUC 0.64–0.69) PTB.ConclusionsOur finding suggests that predictive models based on clinical variables will not be useful to discriminate patients with culture-negative PTB from patients with culture-positive PTB among patients with smear-negative cases.

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Frequency and patterns of first- and second-line drug resistance-conferring mutations in Mycobacterium tuberculosis isolated from pulmonary tuberculosis patients in a cross-sectional study in Tigray Region, Ethiopia

Journal of Global Antimicrobial Resistance ◽

10.1016/j.jgar.2020.11.017 ◽

2021 ◽

Vol 24 ◽

pp. 6-13

Author(s):

Letemichael Negash Welekidan ◽

Eystein Skjerve ◽

Tsehaye Asmelash Dejene ◽

Mengistu Welday Gebremichael ◽

Ola Brynildsrud ◽

...

Keyword(s):

Drug Resistance ◽

Mycobacterium Tuberculosis ◽

Pulmonary Tuberculosis ◽

Cross Sectional Study ◽

Second Line ◽

Sectional Study ◽

Cross Sectional ◽

Tuberculosis Patients ◽

Tigray Region ◽

Line Drug

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Profile of glycated-hemoglobin, antioxidant vitamin and cytokine levels in pulmonary tuberculosis patients: A cross sectional study at Pulmonary Diseases Center Semarang City, Indonesia

Biomedical Journal ◽

10.1016/j.bj.2016.01.011 ◽

2016 ◽

Vol 39 (5) ◽

pp. 354-360 ◽

Cited By ~ 4

Author(s):

Praba Ginandjar ◽

Lintang Dian Saraswati ◽

Bagoes Widjanarko

Keyword(s):

Pulmonary Tuberculosis ◽

Glycated Hemoglobin ◽

Cross Sectional Study ◽

Pulmonary Diseases ◽

Antioxidant Vitamin ◽

Sectional Study ◽

Cross Sectional ◽

Tuberculosis Patients ◽

Cytokine Levels

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A CROSS SECTIONAL STUDY ON KNOWLEDGE OF TUBERCULOISIS AMONG RURAL AND URBAN POPULATION OF UDAIPUR DISTRICT, RAJASTHAN

10.36106/gjra/1301996 ◽

2020 ◽

pp. 1-2

Author(s):

Ganesh Salvi ◽

Rakhi Luthra

Keyword(s):

Risk Factors ◽

Pulmonary Tuberculosis ◽

Urban Population ◽

Cross Sectional Study ◽

Sectional Study ◽

Duration Of Treatment ◽

Cross Sectional ◽

Rural And Urban ◽

Tuberculosis Risk

AIM: To assess the knowledge regarding Tuberculosis cause, spread, types and duration of treatment among rural and urban population of Udaipur, Rajasthan. Methodology: A cross sectional study was conducted during January to March 2020 in OPD’s of PHC and UHC of Udaipur city. Total of 216 participants were included. Results: Our study showed that 30.09% were believed that Tb spread form Air, 42.22% were aware about Pulmonary tuberculosis and 53.24% were know that treatment of Tb lasts for 6 months. Conclusion: There is a need to aware people more about Tuberculosis risk factors, symptoms and treatment.

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