scholarly journals A synthetic biology approach to self-regulatory recombinant protein production in Escherichia coli

2012 ◽  
Vol 6 (1) ◽  
Author(s):  
Martin Dragosits ◽  
Daniel Nicklas ◽  
Ilias Tagkopoulos
Keyword(s):  
Escherichia Coli ◽  
Synthetic Biology ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Synthetic Biology Approach

scholarly journals Isolating Escherichia coli strains for recombinant protein production

2016 ◽  
Vol 74 (5) ◽  
pp. 891-908 ◽  
Author(s):  
Susan Schlegel ◽  
Pierre Genevaux ◽  
Jan-Willem de Gier
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production

scholarly journals Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases

2019 ◽  
Vol 116 (6) ◽  
pp. 1259-1268 ◽  
Author(s):  
Martin Lemmerer ◽  
Juergen Mairhofer ◽  
Alexander Lepak ◽  
Karin Longus ◽  
Rainer Hahn ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Functional Expression ◽  
Coli Cell

scholarly journals Recombinant protein production in an Escherichia coli reduced genome strain

2007 ◽  
Vol 9 (2) ◽  
pp. 133-141 ◽  
Author(s):  
Shamik S. Sharma ◽  
Frederick R. Blattner ◽  
Sarah W. Harcum
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Reduced Genome

scholarly journals Improved designs for pET expression plasmids increase protein production yield in Escherichia coli

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Patrick J. Shilling ◽  
Kiavash Mirzadeh ◽  
Alister J. Cumming ◽  
Magnus Widesheim ◽  
Zoe Köck ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Production Yield ◽  
Expression Plasmids ◽  
Report Design

AbstractThe pET series of expression plasmids are widely used for recombinant protein production in Escherichia coli. The genetic modules controlling transcription and translation in these plasmids were first described in the 1980s and have not changed since. Herein we report design flaws in these genetic modules. We present improved designs and demonstrate that, when incorporated into pET28a, they support increases in protein production. The improved designs are applicable to most of the 103 vectors in the pET series and can be easily implemented.

Sign in / Sign up

Export Citation Format

Share Document