Cloning of a new glutathione peroxidase gene from tea plant (Camellia sinensis) and expression analysis under biotic and abiotic stresses

WRKY transcription factors (TFs) containing one or two WRKY domains are a class of plant TFs that respond to diverse abiotic stresses and are associated with developmental processes. However, little has been known about the function of WRKY gene in tea plant. In this study, a subgroup IId WRKY gene CsWRKY7 was isolated from Camellia sinensis, which displayed amino acid sequence homology with Arabidopsis AtWRKY7 and AtWRKY15. Subcellular localization prediction indicated that CsWRKY7 localized to nucleus. Cis-acting elements detected in the promotor region of CsWRKY7 are mainly involved in plant response to environmental stress and growth. Consistently, expression analysis showed that CsWRKY7 transcripts responded to NaCl, mannitol, PEG, and diverse hormones treatments. Additionally, CsWRKY7 exhibited a higher accumulation both in old leaves and roots compared to bud. Seed germination and root growth assay indicated that overexpressed CsWRKY7 in transgenic Arabidopsis was not sensitive to NaCl, mannitol, PEG, and low concentration of ABA treatments. CsWRKY7 overexpressing Arabidopsis showed a late-flowering phenotype under normal conditions compared to wild type. Furthermore, gene expression analysis showed that the transcription levels of the flowering time integrator gene FLOWERING LOCUS T (FT) and the floral meristem identity genes APETALA1 (AP1) and LEAFY (LFY) were lower in WRKY7-OE than in the WT. Taken together, these findings indicate that CsWRKY7 TF may participate in plant growth. This study provides a potential strategy to breed late-blooming tea cultivar.

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Identification, characterization and expression analysis of the VQ motif-containing gene family in tea plant (Camellia sinensis)

BMC Genomics ◽

10.1186/s12864-018-5107-x ◽

2018 ◽

Vol 19 (1) ◽

Cited By ~ 10

Author(s):

Junhong Guo ◽

Jiangfei Chen ◽

Jiankun Yang ◽

Youben Yu ◽

Yajun Yang ◽

...

Keyword(s):

Gene Family ◽

Expression Analysis ◽

Camellia Sinensis ◽

Tea Plant

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Isolation and expression analysis of 18 CsbZIP genes implicated in abiotic stress responses in the tea plant (Camellia sinensis)

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2015.10.030 ◽

2015 ◽

Vol 97 ◽

pp. 432-442 ◽

Cited By ~ 23

Author(s):

Hongli Cao ◽

Lu Wang ◽

Chuan Yue ◽

Xinyuan Hao ◽

Xinchao Wang ◽

...

Keyword(s):

Abiotic Stress ◽

Expression Analysis ◽

Camellia Sinensis ◽

Stress Responses ◽

Tea Plant

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Genome-Wide Analysis Reveals Stress and Hormone Responsive Patterns of JAZ Family Genes in Camellia Sinensis

International Journal of Molecular Sciences ◽

10.3390/ijms21072433 ◽

2020 ◽

Vol 21 (7) ◽

pp. 2433 ◽

Cited By ~ 1

Author(s):

Jiazhi Shen ◽

Zhongwei Zou ◽

Hongqing Xing ◽

Yu Duan ◽

Xujun Zhu ◽

...

Keyword(s):

Camellia Sinensis ◽

Abiotic Stresses ◽

Synergistic Effects ◽

Tea Plant ◽

Plant Genome ◽

Pcr Analysis ◽

Limited Information ◽

Genome Database ◽

Recombinant Cells ◽

Genome Wide

JAZ (Jasmonate ZIM-domain) proteins play pervasive roles in plant development and defense reaction. However, limited information is known about the JAZ family in Camellia sinensis. In this study, 12 non-redundant JAZ genes were identified from the tea plant genome database. Phylogenetic analysis showed that the 12 JAZ proteins belong to three groups. The cis-elements in promoters of CsJAZ genes and CsJAZ proteins interaction networks were also analyzed. Quantitative RT–PCR analysis showed that 7 CsJAZ genes were preferentially expressed in roots. Furthermore, the CsJAZ expressions were differentially induced by cold, heat, polyethylene glycol (PEG), methyl jasmonate (MeJA), and gibberellin (GA) stimuli. The Pearson correlations analysis based on expression levels showed that the CsJAZ gene pairs were differentially expressed under different stresses, indicating that CsJAZs might exhibit synergistic effects in response to various stresses. Subcellular localization assay demonstrated that CsJAZ3, CsJAZ10, and CsJAZ11 fused proteins were localized in the cell nucleus. Additionally, the overexpression of CsJAZ3, CsJAZ10, and CsJAZ11 in E. coli enhanced the growth of recombinant cells under abiotic stresses. In summary, this study will facilitate the understanding of the CsJAZ family in Camellia sinensis and provide new insights into the molecular mechanism of tea plant response to abiotic stresses and hormonal stimuli.

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