scholarly journals Comparative proteomic analysis of tear fluid versus nasal mucus in allergic rhinitis patients and healthy controls

2013 ◽  
Vol 3 (S2) ◽  
Author(s):  
Peter Valentin Tomazic ◽  
Ruth Birner-Grünberger ◽  
Britta Obrist ◽  
Hanno Wolf ◽  
Doris Lang-Loidolt
Keyword(s):  
Allergic Rhinitis ◽  
Proteomic Analysis ◽  
Tear Fluid ◽  
Healthy Controls ◽  
Comparative Proteomic Analysis ◽  
Nasal Mucus

Comparative proteomic analysis of tear fluid in Graves' disease with and without orbitopathy

2016 ◽  
Vol 85 (5) ◽  
pp. 805-812 ◽  
Author(s):  
C. Aass ◽  
I. Norheim ◽  
E. F. Eriksen ◽  
E. C. Børnick ◽  
P. M. Thorsby ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Tear Fluid ◽  
Graves Disease ◽  
Comparative Proteomic Analysis

scholarly journals Comparative proteomic analysis of skin fibroblasts of patients with familial Alzheimer's and healthy controls: Searching for new therapeutic targets

2020 ◽  
Vol 16 (S4) ◽  
Author(s):  
Gustavo Lopez‐Toledo ◽  
Maria del Carmen Silva‐Lucero ◽  
Jose Antonio Arias‐Montaño ◽  
Mariaarmen del CCardenas‐Aguayo
Keyword(s):  
Proteomic Analysis ◽  
Therapeutic Targets ◽  
Skin Fibroblasts ◽  
Healthy Controls ◽  
Comparative Proteomic Analysis ◽  
New Therapeutic Targets

scholarly journals The acute phase response protein SERPINA3 is increased in tear fluid from the unaffected eyes of patients with unilateral acute anterior uveitis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jon Roger Eidet ◽  
Maja Akopian ◽  
Ole K. Olstad ◽  
Øystein Kalsnes Jørstad ◽  
Morten C. Moe ◽  
...  
Keyword(s):  
Acute Phase ◽  
Anterior Uveitis ◽  
Acute Phase Response ◽  
Phase Response ◽  
Tear Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Bacterial Keratitis ◽  
Acute Anterior Uveitis ◽  
Potential Biomarker

Abstract Background To identify candidate tear fluid biomarkers in patients with unilateral acute anterior uveitis (AAU) that can aid in the differentiation between these patients and patients with bacterial keratitis or healthy controls. Methods Thirteen patients (40.1 ± 16.2 years of age) with unilateral AAU, seven patients with unilateral bacterial keratitis (40.2 ± 15.3 years of age), and 14 healthy subjects (41.1 ± 11.6 years of age) were included. The tear proteome of affected eyes was compared with that of the unaffected eye or healthy controls. Proteins were identified by liquid chromatography tandem mass spectrometry and enzyme-linked immunosorbent assay. Results Relative protein ratios were detected and calculated for 272 unique proteins. Compared with healthy controls and the unaffected eye, the top upregulated proteins in AAU eyes were submaxillary gland androgen regulated protein 3B (SMR3B) and SMR3A. Similarly, the top upregulated proteins in bacterial keratitis were S100 calcium-binding protein A9 and orosomucoid 2. The acute phase response protein Serpin Family A Member 3 (SERPINA3) was increased in the healthy eye of AAU patients (P = 0.019) compared with healthy controls. Laser flare measurements in affected eyes of AAU patients showed positive logarithmic correlation with SERPINA3 in tear samples of the unaffected eye (P = 0.022). The use of SERPINA3 as a tear biomarker yielded a sensitivity of 85% and a specificity of 71% in detecting patients with AAU in the study population. Conclusions The acute phase response protein SERPINA3 was increased in tear samples of unaffected eyes of patients with unilateral AAU compared with healthy controls. This study highlights SERPINA3 as a potential biomarker for AAU. Future research should explore the dynamic properties of SERPINA3 in the tear fluid of active and quiescent uveitis eyes.

scholarly journals Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

2021 ◽  
Vol 22 (12) ◽  
pp. 6323
Author(s):  
Alexander L. Rusanov ◽  
Peter M. Kozhin ◽  
Olga V. Tikhonova ◽  
Victor G. Zgoda ◽  
Dmitry S. Loginov ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Living Organism ◽  
Peritoneal Macrophages ◽  
In Vitro Models ◽  
Individual Characteristics ◽  
Essential Proteins ◽  
Comparative Proteomic Analysis ◽  
Immortalized Cell ◽  
The Individual

In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.

scholarly journals Comparative proteomic analysis of mitochondria isolated from Euglena gracilis under aerobic and hypoxic conditions

PLoS ONE ◽  
2019 ◽  
Vol 14 (12) ◽  
pp. e0227226 ◽  
Author(s):  
Shun Tamaki ◽  
Kohei Nishino ◽  
Takahisa Ogawa ◽  
Takanori Maruta ◽  
Yoshihiro Sawa ◽  
...  
Keyword(s):  
Euglena Gracilis ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis ◽  
Hypoxic Conditions

Diagnosing Nasal Hyperreactivity with Positional Rhinomanometry

1996 ◽  
Vol 105 (11) ◽  
pp. 901-904 ◽  
Author(s):  
Guido Altissimi ◽  
Marco Rossetti ◽  
Luigi Gallucci ◽  
Costantino Simoncelli
Keyword(s):  
Allergic Rhinitis ◽  
Functional System ◽  
Nasal Fossa ◽  
Nasal Resistance ◽  
Healthy Controls ◽  
Perennial Allergic Rhinitis ◽  
Average Percentage ◽  
Nasal Hyperreactivity ◽  
Vasomotor Activity

Positional rhinomanometry is a physiologic method for estimating nasal resistance, which is variable, difficult to predict, and linked to vasomotor activity of the functional system culminating in the turbinate valves. Thirty subjects suffering from allergic rhinitis, 25 patients affected by aspecific rhinitis, and 40 healthy controls underwent positional rhinomanometry. Test positions included the seated (baseline), supine, and recumbent (homolateral and contralateral to the nasal fossa under examination). In patients with perennial allergic rhinitis and in those with aspecific rhinitis, positional rhinomanometry elicited two pathologic responses: either an average percentage rise of more than 80% in nasal resistance in the supine and homolateral and contralateral recumbent positions compared with basal values, or a paradoxical fall in the supine and homolateral recumbent positions.

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