scholarly journals Regulation of the antennal transcriptome of the dengue vector, Aedes aegypti, during the first gonotrophic cycle

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Sharon Rose Hill ◽  
Tanvi Taparia ◽  
Rickard Ignell
Keyword(s):  
Aedes Aegypti ◽  
Disease Transmission ◽  
Transcript Abundance ◽  
Gonotrophic Cycle ◽  
Differential Modulation ◽  
Dengue Vector ◽  
Regulatory Pathways ◽  
Future Studies ◽  
State Dependent ◽  
Insight Into

Abstract Background In the light of dengue being the fastest growing transmissible disease, there is a dire need to identify the mechanisms regulating the behaviour of the main vector Aedes aegypti. Disease transmission requires the female mosquito to acquire the pathogen from a blood meal during one gonotrophic cycle, and to pass it on in the next, and the capacity of the vector to maintain the disease relies on a sustained mosquito population. Results Using a comprehensive transcriptomic approach, we provide insight into the regulation of the odour-mediated host- and oviposition-seeking behaviours throughout the first gonotrophic cycle. We provide clear evidence that the age and state of the female affects antennal transcription differentially. Notably, the temporal- and state-dependent patterns of differential transcript abundance of chemosensory and neuromodulatory genes extends across families, and appears to be linked to concerted differential modulation by subsets of transcription factors. Conclusions By identifying these regulatory pathways, we provide a substrate for future studies targeting subsets of genes across disparate families involved in generating key vector behaviours, with the goal to develop novel vector control tools.

scholarly journals Plant nutrient quality impacts survival and reproductive fitness of the dengue vector Aedes aegypti

2020 ◽  
Author(s):  
Vincent Odhiambo Nyasembe ◽  
David Poumo Tchouassi ◽  
Martha Njeri Muturi ◽  
Christian W. W. Pirk ◽  
Catherine L. Sole ◽  
...  
Keyword(s):  
Amino Acid ◽  
Aedes Aegypti ◽  
Disease Transmission ◽  
Acid Content ◽  
Amino Acid Content ◽  
Reproductive Fitness ◽  
The Other ◽  
Gut Contents ◽  
Dengue Vector

Abstract Background: In a recent study using DNA barcoding, we identified the plants fed upon by four Afro-tropical mosquito species that vector dengue, malaria, and Rift Valley fever. Herein, we have expanded on this study by investigating the role of three of the plants Pithecellobium dulce (Fabaceae), Leonotis nepetifolia (Lamiaceae), and Opuntia ficus-indica (Cactaceae) on survival, fecundity, and egg viability of the dengue vector Aedes aegypti. Methods: We tested these effects using females that received a) an initial three rations of bloodmeal, and b) received no bloodmeal at all. Two controls were included; age-matched females fed on glucose solution with or without initial bloodmeal, and those fed exclusively on bloodmeal. Data was collected daily over a 30-day period. The amino acid content of Ae. aegypti guts and the amino acid content of their respective diets was detected by coupled liquid chromatography – mass spectrometry.Results: Females fed on P. dulce and exclusive bloodmeal had a shorter survival than those fed on glucose. On the other hand, females fed on L. nepetifolia survived longer than those fed exclusively on bloodmeal, whereas those fed on O. ficus-indica had the shortest survival time. With initial bloodmeal, females fed on L. nepetifolia laid 1.6-fold more eggs while those fed on the other diets laid fewer eggs, compared to those fed exclusively on bloodmeal. Hatching rates of the eggs laid varied with the diet. Mass spectroscopic analysis of gut contents of mosquitoes exposed to the different diets showed qualitative and quantitative differences in their amino acid levels. Conclusion: Our findings highlight the central role of plant nutrients in the reproductive fitness of dengue vectors which may impact their disease transmission potential.

scholarly journals Plant Nutrient Quality Promotes Survival and Reproductive Fitness of the Dengue Vector Aedes Aegypti

2020 ◽  
Author(s):  
Vincent Odhiambo Nyasembe ◽  
David P. Tchouassi ◽  
Martha N. Muturi ◽  
Christian W. W. Pirk ◽  
Catherine L. Sole ◽  
...  
Keyword(s):  
Amino Acid ◽  
Aedes Aegypti ◽  
Disease Transmission ◽  
Acid Content ◽  
Amino Acid Content ◽  
Reproductive Fitness ◽  
The Other ◽  
Gut Contents ◽  
Dengue Vector

Abstract Background: In a recent study using DNA barcoding, we identified the plants fed upon by four Afro-tropical mosquito species that vector dengue, malaria, and Rift Valley fever. Herein, we have expanded on this study by investigating the role of three of the plants Pithecellobium dulce (Fabaceae), Leonotis nepetifolia (Lamiaceae), and Opuntia ficus-indica (Cactaceae) on survival, fecundity, and egg viability of the dengue vector Aedes aegypti. Methods: We tested these effects using females that received a) an initial three rations of bloodmeal, and b) received no bloodmeal at all. Two controls were included; age-matched females fed on glucose solution with or without initial bloodmeal, and those fed exclusively on bloodmeal. Data was collected daily over a 30-day period. The amino acid content of Ae. aegypti guts and the amino acid content of their respective diets was detected by coupled liquid chromatography – mass spectrometry.Results: Females fed on P. dulce and exclusive bloodmeal had a shorter survival than those fed on glucose. On the other hand, females fed on L. nepetifolia survived longer than those fed exclusively on bloodmeal, whereas those fed on O. ficus-indica had the shortest survival time. With initial bloodmeal, females fed on L. nepetifolia laid 1.6-fold more eggs while those fed on the other diets laid fewer eggs, compared to those fed exclusively on bloodmeal. Hatching rates of the eggs laid varied with the diet. Mass spectroscopic analysis of gut contents of mosquitoes exposed to the different diets showed qualitative and quantitative differences in their amino acid levels. Conclusion: Our findings highlight the central role of plant nutrients in the reproductive fitness of dengue vectors which may impact their disease transmission potential.

scholarly journals Plant nutrient quality promotes survival and reproductive fitness of the dengue vector Aedes aegypti

2020 ◽  
Author(s):  
Vincent Odhiambo Nyasembe ◽  
David P. Tchouassi ◽  
Martha N. Muturi ◽  
Christian W. W. Pirk ◽  
Catherine L. Sole ◽  
...  
Keyword(s):  
Amino Acid ◽  
Aedes Aegypti ◽  
Disease Transmission ◽  
Acid Content ◽  
Amino Acid Content ◽  
Reproductive Fitness ◽  
The Other ◽  
Gut Contents ◽  
Dengue Vector

Abstract Background In a recent study using DNA barcoding, we identified the plants fed upon by four Afro-tropical mosquito species that vector dengue, malaria, and Rift Valley fever. Herein, we have expanded on this study by investigating the role of three of the plants Pithecellobium dulce (Fabaceae), Leonotis nepetifolia (Lamiaceae), and Opuntia ficus-indica (Cactaceae) on survival, fecundity, and egg viability of the dengue vector Aedes aegypti. Methods We tested these effects using females that received a) an initial three rations of bloodmeal, and b) received no bloodmeal at all. Two controls were included; age-matched females fed on glucose solution with or without initial bloodmeal, and those fed exclusively on bloodmeal. Data was collected daily over a 30-day period. The amino acid content of Ae. aegypti guts and the amino acid content of their respective diets was detected by coupled liquid chromatography – mass spectrometry. Results Females fed on P. dulce and exclusive bloodmeal had a shorter survival than those fed on glucose. On the other hand, females fed on L. nepetifolia survived longer than those fed exclusively on bloodmeal, whereas those fed on O. ficus-indica had the shortest survival time. With initial bloodmeal, females fed on L. nepetifolia laid 1.6-fold more eggs while those fed on the other diets laid fewer eggs, compared to those fed exclusively on bloodmeal. Hatching rates of the eggs laid varied with the diet. Mass spectroscopic analysis of gut contents of mosquitoes exposed to the different diets showed qualitative and quantitative differences in their amino acid levels. Conclusion Our findings highlight the central role of plant nutrients in the reproductive fitness of dengue vectors which may impact their disease transmission potential.

scholarly journals Sex, age, and parental harmonic convergence behavior affect the immune performance of Aedes aegypti offspring

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Christine M. Reitmayer ◽  
Ashutosh K. Pathak ◽  
Laura C. Harrington ◽  
Melinda A. Brindley ◽  
Lauren J. Cator ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Disease Transmission ◽  
Female Offspring ◽  
Physiological Status ◽  
Offspring Performance ◽  
Selective Pressures ◽  
Wide Range ◽  
Harmonic Convergence ◽  
And Control ◽  
Insight Into

AbstractHarmonic convergence is a potential cue, female mosquitoes use to choose male mates. However, very little is known about the benefits this choice confers to offspring performance. Using Aedes aegypti (an important vector of human disease), we investigated whether offspring of converging parental pairs showed differences in immune competence compared to offspring derived from non-converging parental pairs. Here we show that harmonic convergence, along with several other interacting factors (sex, age, reproductive, and physiological status), significantly shaped offspring immune responses (melanization and response to a bacterial challenge). Harmonic convergence had a stronger effect on the immune response of male offspring than on female offspring. Further, female offspring from converging parental pairs disseminated dengue virus more quickly than offspring derived from non-converging parental pairs. Our results provide insight into a wide range of selective pressures shaping mosquito immune function and could have important implications for disease transmission and control.

Laboratory Oviposition Choice of Aedes aegypti (Diptera: Culicidae) From Kenya and Gabon: Effects of Conspecific Larvae, Salinity, Shading, and Microbiome

2021 ◽  
Author(s):  
Siyang Xia
Keyword(s):  
Aedes Aegypti ◽  
Disease Transmission ◽  
Mosquito Control ◽  
Oviposition Behavior ◽  
Oviposition Choice ◽  
African Populations ◽  
Primary Vector ◽  
Insight Into ◽  
Forest Habitats ◽  
Oviposition Cues

Abstract The mosquito Aedes aegypti (L.) is the primary vector of several arboviruses. Mosquito control and surveillance are essential to restrict disease transmission, the effectiveness of which depends on our understanding of the mosquito’s behaviors, including oviposition. Previous studies have identified a variety of oviposition cues. However, most of these studies involved only Ae. aegypti outside of the species’ native range, Africa. Populations outside Africa differ in their genetics and some behaviors from their African counterparts, suggesting possibly different oviposition preferences. Within Africa, Ae. aegypti can be found in both ancestral forest habitats and domestic habitats. The African domestic populations may represent an intermediate state between the forest and the truly domesticated non-African populations. Comparing mosquitoes from these three habitats (African forest, African domestic, and non-African domestic) might provide insight into the evolution of oviposition behavior. In this study, I examined the oviposition choices of multiple Ae. aegypti colonies from all three habitats in laboratory settings. I applied a two-choice assay to test four oviposition cues: the preexistence of conspecific larvae, salinity, shading, and microbiome. A subset of African colonies showed similar oviposition choices as their non-African counterparts, whereas the rest show little response to the factors tested. Within the African colonies, oviposition choices of the domestic colonies were significantly different from the forest colonies in most experiments. Yet, their preferences were not always intermediate between that of mosquitoes from the other two habitats. Collectively, this study adds to our understanding of Ae. aegypti oviposition, especially in previously understudied African populations.

Development and Evaluation of a Pyriproxyfen-treated Device to Control the Dengue Vector, Aedes aegypti (L.) (Diptera: Culicidae)

2013 ◽  
Author(s):  
Alongkot Ponlawat ◽  
Thanyalak Fansiri ◽  
Somwang Kurusarttra ◽  
Arissra Pongsiri ◽  
Patrick W. McCardle ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Vector

Molecular mechanisms associated with increased tolerance to the neonicotinoid insecticide imidacloprid in the dengue vector Aedes aegypti

2013 ◽  
Vol 126 ◽  
pp. 326-337 ◽  
Author(s):  
Muhammad Asam Riaz ◽  
Alexia Chandor-Proust ◽  
Chantal Dauphin-Villemant ◽  
Rodolphe Poupardin ◽  
Christopher M. Jones ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Molecular Mechanisms ◽  
Dengue Vector ◽  
Neonicotinoid Insecticide

scholarly journals Comparisons among rainbow trout, Oncorhynchus mykiss, populations of maternal transcript profile associated with egg viability

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Gregory M. Weber ◽  
Jill Birkett ◽  
Kyle Martin ◽  
Doug Dixon ◽  
Guangtu Gao ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Egg Quality ◽  
Transcript Abundance ◽  
Differentially Expressed ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Analysis Group ◽  
Maternal Transcripts ◽  
Single Batch ◽  
Insight Into

Abstract Background Transcription is arrested in the late stage oocyte and therefore the maternal transcriptome stored in the oocyte provides nearly all the mRNA required for oocyte maturation, fertilization, and early cleavage of the embryo. The transcriptome of the unfertilized egg, therefore, has potential to provide markers for predictors of egg quality and diagnosing problems with embryo production encountered by fish hatcheries. Although levels of specific transcripts have been shown to associate with measures of egg quality, these differentially expressed genes (DEGs) have not been consistent among studies. The present study compares differences in select transcripts among unfertilized rainbow trout eggs of different quality based on eyeing rate, among 2 year classes of the same line (A1, A2) and a population from a different hatchery (B). The study compared 65 transcripts previously reported to be differentially expressed with egg quality in rainbow trout. Results There were 32 transcripts identified as DEGs among the three groups by regression analysis. Group A1 had the most DEGs, 26; A2 had 15, 14 of which were shared with A1; and B had 12, 7 of which overlapped with A1 or A2. Six transcripts were found in all three groups, dcaf11, impa2, mrpl39_like, senp7, tfip11 and uchl1. Conclusions Our results confirmed maternal transcripts found to be differentially expressed between low- and high-quality eggs in one population of rainbow trout can often be found to overlap with DEGs in other populations. The transcripts differentially expressed with egg quality remain consistent among year classes of the same line. Greater similarity in dysregulated transcripts within year classes of the same line than among lines suggests patterns of transcriptome dysregulation may provide insight into causes of decreased viability within a hatchery population. Although many DEGs were identified, for each of the genes there is considerable variability in transcript abundance among eggs of similar quality and low correlations between transcript abundance and eyeing rate, making it highly improbable to predict the quality of a single batch of eggs based on transcript abundance of just a few genes.

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