scholarly journals Characterisation of novel endogenous geminiviral elements in macadamia

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Mohamed C. M. Zakeel ◽  
Andrew D. W. Geering ◽  
John E. Thomas ◽  
Olufemi A. Akinsanmi

Abstract Background The presence of geminivirus sequences in a preliminary analysis of sRNA sequences from the leaves of macadamia trees with abnormal vertical growth (AVG) syndrome was investigated. Results A locus of endogenous geminiviral elements (EGE) in the macadamia genome was analysed, and the sequences revealed a high level of deletions and/or partial integrations, thus rendering the EGE transcriptionally inactive. The replication defective EGE in the macadamia genome indicates its inability to be the source of new viral infections and thus cause AVG or any other disease in macadamia. The EGE sequences were detected in two edible Macadamia species that constitute commercial cultivars and the wild germplasm of edible and inedible species of Macadamia. This strongly suggests that the integration preceded speciation of the genus Macadamia. A draft genome of a locus of EGE in Macadamia was developed. The findings of this study provide evidence to suggest the endogenization of the geminiviral sequences in the macadamia genome and the ancestral relationship of EGE with Macadamia in the Proteaceae family. Random mutations accumulating in the EGE inform that the sequence is evolving. Conclusions The EGE in Macadamia is inactive and thus not a direct cause of any diseases or syndromes including AVG in macadamia. The insertion of the EGE in the macadamia genome preceded speciation of the genus Macadamia.

2021 ◽  
Vol 15 (6) ◽  
pp. 1634-1640
Author(s):  
Javad Jafari ◽  
Asra Nassehi ◽  
Mohammadali Zareez ◽  
Seydamalek Dadkhah ◽  
Najmeh Saberi ◽  
...  

Background: Among all aspects of nursing care, the spiritual one is the issue that has received little attention. Having spiritual wellbeing (SWB) is a necessity to provide appropriate spiritual care. In addition to, the Emotional intelligence (EI) is one of the most important factors in social and professional success and is essential for effective nursing practice. Therefore, aim of study was evaluating the Relationship between SWB and EI among nursing students. Methods: The sample of this descriptive-analytic study consisted of 136 nursing students studying at Bam University of Medical Sciences selected by convenience sampling method. The Bradberry and Greaves 28-item EI scale, Palutzian and Ellison SWB Scale were used to assess the total score of EI and SWB. Collected data were analyzed using descriptive statistics (mean and standard deviation), Pearson correlation coefficient, independent t-test and one-way ANOVA with SPSS v18. Results: The mean score of SWB and EI were 97.1±11.56 and 123.4 + 123.6, respectively. The mean score dimensions of SWB include (religious wellbeing 47.9±6.6, existential wellbeing 49.1±5.7) and dimensions EI: self-awareness, self-management, social awareness, and relationship management were 27.2 3 3.2, 36.2 2 5.4, 25.1 + 3.5 and 35.1 + 4.5, respectively. The majority of students have reported moderate level SWB and high level of emotional intelligence. The factors influencing their level of SWB were academic semester and age (p<0.05). Conclusion: Although the level of students' EI and SWB were at a desirable level in this study, due to the nature of nursing and the interaction between nurses and patients, providing a suitable learning environment for the development of EI is essential. Therefore, it is suggested that nursing policymakers should develop appropriate educational programs for nurses and provide curriculum for students to promote their knowledge and skills. Keywords: spiritual, religious, wellbeing, existential, nursing, emotional intelligence


Author(s):  
Zack Darby ◽  
Neelam Chandra Poudyal ◽  
Adam Frakes ◽  
Omkar Joshi

Municipal drawdowns at public reservoirs can negatively impact recreational uses on site. Therefore, sustaining recreation requires understanding how users relate themselves with the reservoir and the resource therein, and how they will respond to circumstances and policies impacting the resource. Researchers use placedbased theory, particularly sense of place (SOP), to assess the user community’s perspective on the natural resource or recreation site of interest. This study utilized visitor survey data (n=282) from Canton Reservoir in Oklahoma to assess visitors’ sense of place (SOP), and to evaluate the relationship of SOP with their acceptability of alternative water allocation strategies and future intention of visiting the reservoir under depleted water conditions. Visitors had a high level of SOP with the reservoir and supported protective water allocation strategies that either favor the retention of water on-site or ensure a fair distribution between recreation and municipal use. Results suggest a positive relationship between visitors' SOP and their intended trips to the reservoir even under depleted water conditions. The findings highlight the psychological, functional, and emotional benefits associated with the recreational use of the Canton Reservoir, which will in turn help managers make more informed and balanced decisions about water conservation and allocation. Insights from this study will also contribute in literature on the sense of place and protective norms and offers several implications for the management of public reservoirs.


Neurology ◽  
2021 ◽  
pp. 10.1212/WNL.0000000000012025
Author(s):  
Shahram Oveisgharan ◽  
Ana W. Capuano ◽  
Sukriti Nag ◽  
Sonal Agrawal ◽  
Lisa L. Barnes ◽  
...  

Objective.We tested the hypothesis that an inverse association exists between diabetes mellitus (DM) and hemoglobin A1C (A1C) with Transactive response DNA binding protein 43 (TDP-43) levels in older adults.Methods.We leveraged antemortem and postmortem data of decedents from three community-based clinical-pathological studies. DM status, A1C levels, and medications for DM were documented annually. TDP-43 cytoplasmic inclusions, evaluated in 6 brain regions using immunohistochemistry, were used to obtain a semiquantitative TDP-43 score (0-5) in each region, and scores were averaged across regions to obtain a TDP-43 severity score. We used linear regressions to test the association of DM and A1C with the TDP-43 severity score.Results.On average, participants (n=817) were 90 years old at the time of death, three fourth were women, and one fourth had DM. The mean A1C was 6.0% (SD=0.6). TDP-43 was observed in 54% of participants, and the mean TDP-43 score was 0.7 (range 0-4.5). A higher level of A1C was associated with a lower TDP-43 score (estimate=-0.156, S.E.=0.060, p=0.009) while DM had a borderline inverse association with the TDP-43 score (estimate=-0.163, S.E.=0.087, p=0.060). The association of higher levels of A1C with lower TDP-43 scores persisted after further adjustment by Apolipoprotein ε4, vascular risk factors, stroke, and hypoglycemic medications. Exclusion of the oldest old participants did not change the results.Conclusion.Overall, the results suggest that a high level of A1C is associated with less TDP-43 proteinopathy in older persons while the relationship of DM with TDP-43 needs further study.


2021 ◽  
pp. 22-28
Author(s):  
А.А. Чаицкий ◽  
Н.С. Баранова

Среди сложных белков молока – казеинов – чаще всего встречаются α- и β-казеин. Ген бета-казеина имеет 12 аллельных вариантов, среди которых получили преимущество варианты А1 и А2. При этом, А2 – естественная для человеческого организма разновидность белка бета-казеина, а тип А1 возник в результате природной генетической мутации. Для получения максимального объёма продукции лучшего качества при переориентации производства на молоко А2 необходимо учитывать как основные количественные показатели продуктивности крупного рогатого скота, так и коэффициенты, определяющие эффективность использования коров с точки зрения биологической полноценности продукции, такие как биологической эффективности коров (БЭК), биологической полноценности молока (КБП), кормового коэффициента (FCR) и валовой эффективности использования кормов (GFE). Представлено сравнение количественных и качественных показателей животных разного возраста и групп с учётом степени кровности по улучшающей породе, а также с учётом генотипа по бета-казеину. Впервые проведён анализ реализации биологического потенциала крупного рогатого скота костромской породы с разными аллельными вариантами гена бета-казеина. В ходе исследования установлено, что животные генотипа А2А2 по бета-казеину отличались высоким уровнем БЭК и КБП, а также характеризовались достоверно более высоким уровнем реализации сухого вещества корма в молоко (FCR, GFE), чем у животных А1А2. Так, удои у коров с генотипом А2А2 с кровностью 50% по улучшающей породе за полновозрастную лактацию на 2514 кг больше, чем у животных генотипа А1А2, а коэффициенты БЭК, КБП и FCRвыше на 39,79%, 15,81% и 0,57 кг соответственно. Among the complex proteins of milk – caseins – most often there are α- and β-casein. The beta-casein gene has 12 allelic variants, among which the A1 and A2 variants have gained advantage. At the same time A2 is a natural variety of beta-casein protein for the human body and type A1 arose as a result of a natural genetic mutation. To obtain the maximum volume of production of better quality when reorienting production to milk A2 it is necessary to take into account both the main quantitative indicators of cattle productivity and the coefficients that determine the efficiency of cows use in terms of biological usefulness of products, such as the biological efficiency of cows (BEC), the biological usefulness of milk (BUC), feed coefficient rate (FCR) and gross feed efficiency (GFE). A comparison of quantitative and qualitative indicators of animals of different ages and groups is presented, taking into account the degree of blood relationship by improving breed, as well as taking into account the genotype by beta-casein. For the first time an analysis of the implementation of the biological potential of cattle of the Kostroma breed with different allelic variants of the beta-casein gene was carried out. During the research it was established that animals of a genotype A2A2 on beta casein differed in the BEC and BUC high level and also were characterized by significantly higher level of implementation of dry matter of feed into milk (FCR, GFE), than at animals A1A2. Thus, yields in cows with a genotype A2A2 with a blood relationship of 50% for the improving breed for full-age lactation 2514 kg more than in animals of the genotype A1A2 and the coefficients of BEC, BUC and FCR are higher by 39.79%, 15.81% and 0.57 kg, respectively. Keywords:


1980 ◽  
Vol 87 (1) ◽  
pp. 180-196 ◽  
Author(s):  
A B Novikoff ◽  
P M Novikoff ◽  
O M Rosen ◽  
C S Rubin

In differentiating 3T3-L1 cells, lipid spheres, the endoplasmic reticulum (ER), microperoxisomes, and mitochondria form "constellations" that may reflect the interplay of lipid metabolizing enzymes in these organelles. ER cisternae are also situated very close to "rosettes,"plasmalemmal specializations found in mature adipocytes in vivo. As in hepatocytes and absorptive cells of the intestine, this spatial relationship of ER and plasmalemma suggests a role for rosettes in the uptake of exogenous lipid precursors. The morphological differentiation of 3T3-L1 preadipocytes includes the loss of "stress fibers" and the appearance of microfilament like structures that encase, in a complex manner, the cytosolic lipid spheres that appear during differentiation. Other features described for the first time in 3T3-L1 preadipocytes include: (a) the presence of an extensive acid phosphatase (AcPase) positive GERL from which coated vesicles apparently arise (these coated vesicles display AcPase activity and are much smaller and far more numerous than the coated vesicles that seem to arise from the plasmalemmal coated pits); (b) the abundance of AcPase-positive autophagic vacuoles; and (c) a high level of alpha-naphthyl-acetate-esterase activity which, by light microscopy cytochemistry, appears to be localized in the cytosol.


2019 ◽  
Vol 3 (Supplement_1) ◽  
pp. S559-S560
Author(s):  
Katherine Kennedy ◽  
John R Bowblis ◽  
Katherine M Abbott

Abstract Stabilizing certified nursing assistant (CNA) employment is necessary for maintaining care networks and providing high quality of care for nursing home (NH) residents. This study’s objective was to examine the relationship of high wages and empowerment practices on CNA retention. We used the 2015 Ohio Biennial Survey to construct a facility-level dataset of 547 NHs and estimated multivariable linear regressions. NHs that provided both high wages and high empowerment were associated with a 12.95 percentage-point improvement in the CNA retention rate (SE = 4.53, t-value = 2.86, p = 0.0045). High wages and a high empowerment score did not have significant effects individually (p &gt; .05). Retention rates were similar between NHs that lacked high wages and scored low on the empowerment scale, and NHs that provided one at a high level but not the other. Implications for better retaining CNAs require multiple empowerment practices combined with high hourly wages.


1982 ◽  
Vol 26 (9) ◽  
pp. 818-822 ◽  
Author(s):  
Mary M. Harbeson ◽  
Robert S. Kennedy ◽  
Michele Krause ◽  
Alvah C. Bittner

Two information processing tasks were considered for inclusion in a test battery which was being developed for repeated measures investigations of adverse environmental effects. The tests, adapted from the Rose Battery were: Baron's Graphemic and Phonemic Analysis, and Posner's Letter Classification. Alternate forms of the the tests were administered on 15 consecutive workdays (Monday to Friday) to 21 Navy enlisted men. A total of 23 measures were taken. These scores were examined to determine when in practice they obtained unchanging or linearly changing means, homogeneous variances, and constant (differentially stable) intertrial correlations of an acceptably high level (task definition). In general, correlations of the basic measures tended to become stable with sufficient practice, but derived measures such as difference, slope, and ratio scores did not attain stability. The Baron and Posner tasks had high reliabilities and were highly correlated with each other. Preliminary analysis indicated that both tests may be measuring the same thing. Either the Baron SN or Posner Name score, test may be recommended for repeated measures experimentation.


Author(s):  
Jie Gao ◽  
Leonidas Guibas

This paper surveys the use of geometric methods for wireless sensor networks. The close relationship of sensor nodes with their embedded physical space imposes a unique geometric character on such systems. The physical locations of the sensor nodes greatly impact on system design in all aspects, from low-level networking and organization to high-level information processing and applications. This paper reviews work in the past 10 years on topics such as network localization, geometric routing, information discovery, data-centric routing and topology discovery.


1983 ◽  
Vol 28 (7) ◽  
pp. 532-535 ◽  
Author(s):  
Gary Taerk

This paper describes the working of an oncology nurses coping group which functioned under the guidance of a consultation-liaison psychiatrist at the Toronto General Hospital. Nurses were helped to deal more effectively with the many and varied stresses which they face in treating patients with cancer. As a result ward atmosphere, patient care and inter-staff communication improved. The success of the group was due to a number of factors that included the high motivation of the staff the high level of stress on the ward, the support of the head nurse and the consistent relationship of the consulting psychiatrist. A series of cases are presented to illustrate these points.


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