scholarly journals Reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Bin Wei ◽  
Ying Guo ◽  
Liangjun Zhang ◽  
Huixiu Zhong ◽  
Qiang Miao ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocyte ◽  
Indirect Method ◽  
Southwest China ◽  
Lymphocyte Subsets ◽  
Cd8 T Cell ◽  
Reference Intervals ◽  
Healthy Population ◽  
Reference Ranges ◽  
T Lymphocyte Subsets

Abstract Background Appropriate reference ranges of T lymphocyte subsets are essential for immune status evaluation of patients with immunological diseases. We aim to establish the age- and sex-related reference intervals of T lymphocyte subsets by single-platform for the southwest China population using the indirect method with the data resulting from 53,822 cases of periodic health examination individuals in the Laboratory Information System (LIS) of West China Hospital from 2018 to 2020. Methods We used the Box-Cox conversion combined with the Tukey method to normalize the data and eliminate the outliers, and the nonparametric method to estimate the 95% distribution reference intervals. Results We initially established the reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China by indirect method (See text for details). Using the standard normal deviate test (z-test) suggested by Harris and Boyd according to CLSI EP28-A3C, which is more scientific, we found the reference ranges of T lymphocyte subsets should be differentiated by ages and genders since the reference ranges of T lymphocyte subsets by single-platform in different ages and genders are significantly different. Conclusions We further demonstrated the absolute count of CD3 + T cell, CD3 + CD4 + T cell, CD3 + CD8 + T cell decreased with aging, which is more marked in men and CD3 + CD8 + T cell count, and the obtained reference intervals were superior to the reference intervals derived from the reagent specification currently in use.

scholarly journals T-lymphocyte subsets in lung transplant recipients: association between nadir CD4 T-cell count and viral infections after transplantation

2015 ◽  
Vol 69 ◽  
pp. 110-116 ◽  
Author(s):  
Sandra A. Calarota ◽  
Antonella Chiesa ◽  
Annalisa De Silvestri ◽  
Monica Morosini ◽  
Tiberio Oggionni ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocyte ◽  
Lung Transplant ◽  
Lymphocyte Subsets ◽  
Viral Infections ◽  
Cd4 T Cell ◽  
Transplant Recipients ◽  
T Lymphocyte Subsets ◽  
Lung Transplant Recipients ◽  
Cd4 T Cell Count

Establishment of Serum TSH Reference Intervals by an Indirect Method for Healthy Population in Southwest China

2021 ◽  
Vol 67 (09/2021) ◽  
Author(s):  
Guangjie Zhang ◽  
Qindong Liang ◽  
Yi Yang ◽  
Ping Leng ◽  
Enping He ◽  
...  
Keyword(s):  
Indirect Method ◽  
Southwest China ◽  
Reference Intervals ◽  
Healthy Population ◽  
Serum Tsh

scholarly journals Establishment of Normal Reference Intervals for CD3+, CD4+, CD8+, and CD4+to CD8+Ratio of T Lymphocytes in HIV Negative Adults from University of Gondar Hospital, North West Ethiopia

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Addisu Gize ◽  
Biniam Mathewos ◽  
Beyene Moges ◽  
Meseret Workineh ◽  
Lealem Gedefaw
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Reference Interval ◽  
Reference Intervals ◽  
University Hospital ◽  
T Lymphocyte Subsets ◽  
Cross Sectional ◽  
North West ◽  
Normal Reference

Background.Reference values for the CD3+, CD4+, CD8+, and CD4+to CD8+ratio T lymphocyte subsets are adopted from textbooks. But for appropriate diagnosis, treatment, and follow-up of patients, correct interpretations of the laboratory results from normal reference interval are mandatory. This study was, therefore, planned to establish normal reference interval for T lymphocytes subset count and CD4+to CD8+ratio.Methods.A cross-sectional study was conducted on apparently healthy adult individuals who visited voluntary counseling and HIV testing clinic Gondar University Hospital from April to May, 2013. Whole blood was analyzed using fluorescence-activated cell sorting (BD FACS, San Jose, CA) machine to enumerate the T-cell subpopulations.Results.Out of the total 320 study participants, 161 (50.3%) were men and 159 (49.7%) were women. The normal reference intervals were (655–2,823 cells/μL), (321–1,389 cells/μL), and (220–1,664 cells/μL) for CD3+, CD4+, and CD8+T lymphocyte subsets, respectively, and CD4+to CD8+ratio was 0.5–2.5.Conclusion.The overall CD3+T lymphocytes reference interval in the current study was wide; low CD4+T lymphocytes, CD4 to CD8 ratio, and high CD8+T lymphocytes values were observed.

scholarly journals Clinical Investigations. T-Lymphocyte Subset Absolute Counts in the Peripheral Blood of Mediterranean Spotted Fever Patients: Relations to Disease Severity / Абсолютное Количество Субпопуляций Т-Лимфоцитов В Перифери- Ческой Крови Пациентов Со Средиземноморской Пятнистой Лихо- Радкой: Установление Связи С Тяжестью Заболевания

Folia Medica ◽  
2015 ◽  
Vol 57 (2) ◽  
pp. 93-103 ◽  
Author(s):  
Ivan G. Baltadzhiev ◽  
Pavel I. Pavlov
Keyword(s):  
T Cell ◽  
Disease Severity ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Spotted Fever ◽  
Lymphocyte Subset ◽  
T Cell Subsets ◽  
Mediterranean Spotted Fever ◽  
T Lymphocyte Subsets ◽  
The Absolute

AbstractINTRODUCTION: Mediterranean spotted fever (MSF) in Bulgaria is caused by Rickettsia conorii conorii. Aim: This study aims at investigating the absolute counts of T-lymphocyte subsets in the peripheral blood of patients with MSF in order to establish relationships with disease severity. MATERIALS AND METHODS: The absolute counts of T-lymphocyte subsets were tested in the blood of 62 patients in the acute stage of MSF. They were assigned into three age and sex matched groups, based on the severity of disease - with mild, moderate or severe forms. Controls were 32 age and sex matched healthy individuals. The diagnosis was confirmed by an immunofluorescence assay. Immunophenotyping was performed using Epics XL-MCL Coulter, USA flow-cytometer. RESULTS: The absolute counts of immune competent (CD3+) cells, as well as the counts of helper/inducer (CD3+CD4+) and suppressor/ cytotoxic (CD3+CD8+) T-cell subsets decreased in parallel with disease severity. Naïve (CD4+CD45RA+) and activated memory (CD4+CD45RO+) T-cell subsets were reduced, particularly in severe MSF. Taken as a whole, the counts of activated (CD3+HLA-DR+) and that of presenting accessory (CD28+) or stimulatory (CD38+) molecules Т-cell subsets was increased, but in the first two subsets the trend from mild to severe forms of the disease was descending. CONCLUSION: Reduced T-lymphocyte subset counts are likely related to trans-migration into perivascular inflammatory foci. The increased number of T-lymphocytes bearing activation molecules reflects a mobilization of the cell-mediated immune response. An important issue of this study is the possible prognostic value of T-cell subsets counting, predicting the evolution of a clinical condition to clinical forms, according to the disease severity.

Changes in L-Selectin Expression by T Lymphocytes after Extracorporeal Photochemotherapy.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 1961-1961
Author(s):  
Manuel Ramirez ◽  
Marta Gonzalez-Vicent ◽  
Antonio Perez-Martinez ◽  
Julian Sevilla ◽  
Luis Madero ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Receptor Activation ◽  
P Value ◽  
T Lymphocyte Subsets ◽  
Extracorporeal Photochemotherapy ◽  
Steroid Refractory

Abstract Extracorporeal photochemotherapy (ECP) is an emerging treatment modality for steroid-refractory acute and chronic graft versus host disease (GVHD). The mechanisms by which ECP works are still not fully understood, and modulation of dendritic cell subpopulations, a shift of cytokine profile from Th1 to Th2 and an increase of T-cell regulatory (Treg) cells have been related to the ECP beneficial effect. Changes on T-lymphocyte subsets other than Treg have been reported after ECP (Biol Blood Marrow Transplant2006; 12(1 Suppl 2):22–30.). We analyzed the effect of ECP on the T lymphocyte subsets of sixteen children receiving this form of therapy. Steroid refractory GVHD was defined as failure to respond to 2 mg/Kg/day of methylprednisolone after 5 days (acute GVHD) or failure to respond to steroids or flare of disease activity upon tapering (chronic GVHD). ECP was performed by an alternative approach to that of the classical UVAR XTS system. This alternative method is based on a continuous-flow cell separator (COBE Spectra) that allow to process more mononuclear cells in a smaller volume and it is less time-consuming, both important advantages for pediatric patients. We studied the L-selectin (CD62L) and the CD45RA expression on CD4 and CD8 lymphocytes by flow cytometry. This combination allowed us to distinguish naive (TN, CD62L+CD45RA+), central memory (TCM, CD62L+CD45RA+), effector memory (TEM, CD62L+CD45RA+), and terminal differentiated T cells (TT, CD62L+CD45RA+), as described in Science2000;290:92–97. We compared the proportion of each of these four subpopulations, as well as the L-selectin positive and L-selectin negative ones, in samples collected from peripheral blood before the first (PRE) and after the last (POST) ECP procedures. Statistical analyses were done by the Wilcoxon signed-rank test. Results are shown in the following tables: CD4 SUBSETS & ECP PRE POST p value TN 6.58 ± 2.39 6.18 ± 2.96 0.2003 TCM 58.17 ± 4.49 43.85 ± 4.78 0.0268 TEM 34.64 ± 4.51 46.86 ± 4.89 0.0356 TT 0.6 ± 0.18 3.11 ± 1.46 0.1704 CD62Lpos 64.76 ± 4.4 50.03 ± 5.45 0.0268 CD62Lneg 35.23 ± 4.4 49.97 ± 5.45 0.0268 CD8 SUBSETS & ECP PRE POST p value TN 16.95 ± 3.94 12.53 ± 4.05 0.2343 TCM 28.8 ± 4.95 12.27 ± 2.86 0.0013 TEM 46.62 ± 5.79 51.4 ± 4.22 0.1477 TT 11.17 ± 3.34 23.52 ± 4.79 0.0105 CD62Lpos 45.75 ± 6.1 24.8 ± 5.34 0.0174 CD62Lneg 53.8 ± 6.07 74.92 ± 5.31 0.0174 The clinical outcome of the patients was always positive, with more than 50% achieving complete remission. The proportion of L-selectin expressing T lymphocytes significantly diminished after ECP, both in CD4 and in CD8 cells. The reason for these changes are currently unknown. L-selectin is an important T-cell homing receptor for T-cell entry into lymph nodes via high endothelial venules. Expression of CD62L is rapidly lost following T-cell receptor activation, leading to exit from the lymph node into the periphery and sites of inflammation. CD62Lneg and CD62Lpos also differ in their functional abilities, such as cytokine secretion and cytolytic potential. Our results suggest that ECP may have an impact in the trafficking patterns of T lymphocytes, redirectioning T cells from lymphoid to extralymphoid organs. In addition, ECP was associated to a redistribution of the pool of non-naive T lymphocytes.

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