scholarly journals An operon consisting of a P-type ATPase gene and a transcriptional regulator gene responsible for cadmium resistances in Bacillus vietamensis 151–6 and Bacillus marisflavi 151–25

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Xiaoxia Yu ◽  
Zundan Ding ◽  
Yangyang Ji ◽  
Jintong Zhao ◽  
Xiaoqing Liu ◽  
...  
Keyword(s):  
Transcriptional Regulator ◽  
Regulator Gene ◽  
Atpase Gene ◽  
P Type

Transcriptional regulation of cytoskeletal functions and segmentation by a novel maternal pair-rule gene, lilliputian

Development ◽  
2001 ◽  
Vol 128 (5) ◽  
pp. 801-813 ◽  
Author(s):  
A.H. Tang ◽  
T.P. Neufeld ◽  
G.M. Rubin ◽  
H.A. Muller
Keyword(s):  
Transcriptional Control ◽  
Fragile X ◽  
Transcriptional Regulator ◽  
Regulator Gene ◽  
Embryonic Patterning ◽  
Fushi Tarazu ◽  
Fragile X Mental Retardation ◽  
Pair Rule Gene ◽  
Mutant Phenotypes ◽  
Pair Rule

Transcriptional control during early Drosophila development is governed by maternal and zygotic factors. We have identified a novel maternal transcriptional regulator gene, lilliputian (lilli), which contains an HMG1 (AT-hook) motif and a domain with similarity to the human fragile X mental retardation FMR2 protein and the AF4 proto-oncoprotein. Embryos lacking maternal lilli expression show specific defects in the establishment of a functional cytoskeleton during cellularization, and exhibit a pair-rule segmentation phenotype. These mutant phenotypes correlate with markedly reduced expression of the early zygotic genes serendipity alpha, fushi tarazu and huckebein, which are essential for cellularization and embryonic patterning. In addition, loss of lilli in adult photoreceptor and bristle cells results in a significant decrease in cell size. Our results indicate that lilli represents a novel pair-rule gene that acts in cytoskeleton regulation, segmentation and morphogenesis.

scholarly journals Activation of PLAG1 and HMGA2 by gene fusions involving the transcriptional regulator gene NFIB

2020 ◽  
Vol 59 (11) ◽  
pp. 652-660 ◽  
Author(s):  
Maryam Kakay Afshari ◽  
André Fehr ◽  
Paloma Tejera Nevado ◽  
Mattias K. Andersson ◽  
Göran Stenman
Keyword(s):  
Transcriptional Regulator ◽  
Gene Fusions ◽  
Regulator Gene

scholarly journals Genetic and Transcriptional Organization of the clpC Locus in Bifidobacterium breve UCC 2003

2005 ◽  
Vol 71 (10) ◽  
pp. 6282-6291 ◽  
Author(s):  
Marco Ventura ◽  
Gerald F. Fitzgerald ◽  
Douwe van Sinderen
Keyword(s):  
Promoter Region ◽  
Specific Binding ◽  
Transcriptional Regulator ◽  
Biologically Active ◽  
Slot Blot ◽  
Bifidobacterium Breve ◽  
Homologous Genes ◽  
Species Analysis ◽  
Atpase Gene

ABSTRACT A homolog of the clpC ATPase gene was identified in the genome of Bifidobacterium breve UCC 2003. Since this gene is very well conserved among eubacteria, we employed a PCR-based approach using primers based on highly conserved regions of ClpC proteins in order to identify homologous genes in other bifidobacterial species. Analysis by slot blot, Northern blot, and primer extension experiments showed that transcription of clpC is induced in response to moderate heat shock regimes. Moreover, we identified in the genome sequence of B. breve UCC 2003 a gene, designated clgR, which is predicted to encode a transcriptional regulator involved in regulation of the bifidobacterial clpC gene. The role of this protein in the regulation of B. breve UCC 2003 clpC gene expression was investigated by performing gel retardation experiments. We show that a biologically active ClgR molecule requires one or more proteinaceous coactivators to assist in the specific binding of ClgR to the clpC promoter region.

Identification and phylogenetic classification of eleven putative P-type calcium transport ATPase genes in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe

1996 ◽  
Vol 16 (2) ◽  
pp. 75-85 ◽  
Author(s):  
P. Catty ◽  
A. Goffeau
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Subcellular Location ◽  
Homologous Proteins ◽  
Yeast Saccharomyces Cerevisiae ◽  
Phylogenetic Classification ◽  
New Class ◽  
Atpase Gene ◽  
Protein Sequence Homology ◽  
P Type

Calcium is an essential second messenger in yeast metabolism and physiology. So far, only four genes coding for calcium translocating ATPases had been discovered in yeast. The recent completion of the yeast Saccharomyces cerevisiae genome allowed us to identify six new putative Ca++-ATPases encoding genes. Protein sequence homology analysis and phylogenetic classification of all putative Ca++-ATPase gene products from the yeasts Saccharomyces cerevisiae and Schizosacchraomyces pombe reveal three clusters of homologous proteins. Two of them comprises seven proteins which might belong to a new class of P-type ATPases of unknown subcellular location and of unknown physiological function.

scholarly journals An operon consisting of a P-type ATPase gene and a transcriptional regulator gene responsible for cadmium resistances in Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25

2020 ◽  
Author(s):  
Xiaoxia Yu ◽  
Zundan Ding ◽  
Yangyang Ji ◽  
Jintong Zhao ◽  
Xiaoqing Liu ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Biological Effects ◽  
Resistance Mechanism ◽  
Transcriptional Regulator ◽  
Cadmium Stress ◽  
Cadmium Resistance ◽  
Toxic Heavy Metal ◽  
Cad System ◽  
Atpase Gene ◽  
Similar Gene

Abstract Background: Cadmium (Cd) is a severely toxic heavy metal to most microorganisms. Many bacteria have developed Cd 2+ resistance. Results: In this study, we isolated two different Cd 2+ resistance Bacillus sp . strains, Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25 , which could be grown in the presence of Cd 2+ at concentration up to 0.3 mM and 0.8 mM, respectively. According to the genomic sequencing, transcriptome analysis under cadmium stress, and other related experiments, a gene cluster in plasmid p25 was found to be a major contributor to Cd 2+ resistance in B. marisflavi 151-25. The cluster in p25 contained orf4802 and orf4803 which encodes an ATPase transporter and a transcriptional regulator protein, respectively. Although 151-6 has much lower Cd 2+ resistance than 151-25, they contained similar gene cluster, but in different locations. A gene cluster on the chromosome containing orf4111 , orf4112 and orf4113 , which encodes an ATPase transporter, a cadmium efflux system accessory protein and a cadmium resistance protein, respectively, was found to play a major role on the Cd 2+ resistance for B. vietamensis 151-6. Conclusions: This work described cadmium resistance mechanisms in newly isolated Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25. Based on homologies to the cad system (CadA-CadC) in Staphylococcus aureus and analysis of transcriptome under Cd 2+ induction, we inferred that the mechanisms of cadmium resistance in B. marisflavi 151-25 was as same as the cad system in S. aureus . Although Bacillus vietamensis 151-6 also had the similar gene cluster to B. marisflavi 151-25 and S. aureus , its transcriptional regulatory mechanism of cadmium resistance was not same. This study explored the cadmium resistance mechanism for B. vietamensis 151-6 and B. marisflavi 151-25 and has expanded our understanding of the biological effects of cadmium.

scholarly journals ATP7B variant penetrance explains differences between genetic and clinical prevalence estimates for Wilson disease

2018 ◽  
Author(s):  
Daniel F. Wallace ◽  
James S. Dooley
Keyword(s):  
Wilson Disease ◽  
Genomic Sequence ◽  
Genetic Disorder ◽  
Copper Metabolism ◽  
Genetic Studies ◽  
Population Prevalence ◽  
Atpase Gene ◽  
Prevalence Estimates ◽  
The Uk ◽  
P Type

AbstractWilson disease (WD) is a genetic disorder of copper metabolism caused by variants in the copper transporting P-type ATPase gene ATP7B. Estimates for WD population prevalence vary with 1 in 30,000 generally quoted. However, some genetic studies have reported much higher prevalence rates. The aim of this study was to estimate the population prevalence of WD and the pathogenicity/penetrance of WD variants by determining the frequency of ATP7B variants in a genomic sequence database. A catalogue of WD-associated ATP7B variants was constructed and then frequency information for these was extracted from the gnomAD dataset. Pathogenicity of variants was assessed by (a) comparing gnomAD allele frequencies against the number of reports for variants in the WD literature and (b) using variant effect prediction algorithms. 231 WD-associated ATP7B variants were identified in the gnomAD dataset, giving an initial estimated population prevalence of around 1 in 2400. After exclusion of WD-associated ATP7B variants with predicted low penetrance, the revised estimate showed a prevalence of around 1 in 20,000, with higher rates in the Asian and Ashkenazi Jewish populations. Reanalysis of other recent genetic studies using our penetrance criteria also predicted lower population prevalences for WD in the UK and France than had been reported. Our results suggest that differences in variant penetrance can explain the discrepancy between reported epidemiological and genetic prevalences of WD. They also highlight the challenge in defining penetrance when assigning causality to some ATP7B variants.

Sign in / Sign up

Export Citation Format

Share Document