scholarly journals Comparative proteomic analysis of Nicotiana benthamiana plants under Chinese wheat mosaic virus infection

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Long He ◽  
Peng Jin ◽  
Xuan Chen ◽  
Tian-Ye Zhang ◽  
Kai-Li Zhong ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Enrichment Analysis ◽  
Mapk Signaling ◽  
Host Proteins ◽  
Proteomics Analysis ◽  
Polymyxa Graminis ◽  
Localization Analysis ◽  
Chinese Wheat ◽  
Mosaic Virus Infection

Abstract Background Chinese wheat mosaic virus (CWMV) is a severe threat to winter wheat and is transmitted by Polymyxa graminis. The mechanisms of interactions between CWMV and plants are poorly understood. In this study, a comparative proteomics analysis based on nanoliquid chromatography mass spectrometry (MS)/MS was conducted to characterize proteomic changes in plants responding to CWMV infection. Results In total, 2751 host proteins were identified, 1496 of which were quantified and 146 up-regulated and 244 down-regulated proteins were identified as differentially expressed proteins (DEPs). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that DEPs were most strongly associated with photosynthesis antenna proteins, MAPK signaling plant and glyoxylate and dicarboxylate metabolism pathways. Subcellular localization analysis predicted that more than half of the DEPs were localized in the chloroplast, an organelle indispensable for abscisic acid (ABA) synthesis. Our results suggest that CWMV infection interrupts normal chloroplast functions and decreases ABA concentrations in Nicotiana benthamiana. Further analysis showed that the ABA pathway was suppressed during CWMV infection and that ABA treatment induced plant hosts defenses against CWMV. Conclusions We identified several candidate proteins expressed during CWMV infection, and the ABA pathway was strongly associated with responses to CWMV infection in N. benthamiana.

Comparative Proteomic Analysis of Nicotiana Benthamiana Seedings Under Chinese Wheat Mosaic Virus Infection

2020 ◽  
Author(s):  
Long He ◽  
Peng Jin ◽  
Xuan Chen ◽  
Tian-Ye Zhang ◽  
Kai-Li Zhong ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Enrichment Analysis ◽  
Mapk Signaling ◽  
Host Proteins ◽  
Proteomics Analysis ◽  
Polymyxa Graminis ◽  
Localization Analysis ◽  
Chinese Wheat ◽  
Mosaic Virus Infection

Abstract Background: Chinese wheat mosaic virus (CWMV) is a severe threat to winter wheat, and it is transmitted by Polymyxa graminis. However, the mechanisms of interactions between CWMV and plants are poorly understood. In this study, a comparative proteomics analysis based on nanoliquid chromatography (LC)- mass spectrometry (MS)/MS technique was conducted to characterize the proteomic changes of plants in response to CWMV infection. Results: A total of 2,751 host proteins were identified, 1,496 of which were quantified. 146 up-regulated and 248 down-regulated proteins were identified as differentially expressed proteins (DEPs). KEGG enrichment analysis showed that the DEPs were most strongly associated with Photosynthesis - antenna proteins, MAPK signaling – plant and Glyoxylate and dicarboxylate metabolism pathways. Subcellular localization analysis showed that more than half of DEPs were predicted to be localized in the chloroplast, an organelle indispensable for abscisic acid (ABA) synthesis. Our results suggested that CWMV infection interrupts normal chloroplast functions and decreased the contents of ABA in Nicotiana benthamiana. Further analysis showed that the ABA pathway was suppressed under CWMV infection and ABA treatment did induce plant hosts defense against CWMV. Conclusions: In summary, our results identified several candidate proteins under CWMV infection, and the ABA pathway was deeply involved in the responses to CWMV infection in N. benthamiana.

scholarly journals Genome-Wide Identification and Characterization of Long Noncoding RNAs Involved in Chinese Wheat Mosaic Virus Infection of Nicotiana benthamiana

Biology ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 232
Author(s):  
Weiran Zheng ◽  
Haichao Hu ◽  
Qisen Lu ◽  
Peng Jin ◽  
Linna Cai ◽  
...  
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Differentially Expressed ◽  
Genome Wide ◽  
Chinese Wheat

Recent studies have shown that a large number of long noncoding RNAs (lncRNAs) can regulate various biological processes in animals and plants. Although lncRNAs have been identified in many plants, they have not been reported in the model plant Nicotiana benthamiana. Particularly, the role of lncRNAs in plant virus infection remains unknown. In this study, we identified lncRNAs in N. benthamiana response to Chinese wheat mosaic virus (CWMV) infection by RNA sequencing. A total of 1175 lncRNAs, including 65 differentially expressed lncRNAs, were identified during CWMV infection. We then analyzed the functions of some of these differentially expressed lncRNAs. Interestingly, one differentially expressed lncRNA, XLOC_006393, was found to participate in CWMV infection as a precursor to microRNAs in N. benthamiana. These results suggest that lncRNAs play an important role in the regulatory network of N. benthamiana in response to CWMV infection.

Nicotiana benthamiana Argonaute10 plays a pro‐viral role in Bamboo mosaic virus infection

2019 ◽  
Vol 224 (2) ◽  
pp. 804-817 ◽  
Author(s):  
Ying Wen Huang ◽  
Chung Chi Hu ◽  
Ching Hsiu Tsai ◽  
Na Sheng Lin ◽  
Yau Heiu Hsu
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Mosaic Virus Infection

Engineered nanomaterials suppress Turnip mosaic virus infection in tobacco (Nicotiana benthamiana)

2018 ◽  
Vol 5 (7) ◽  
pp. 1685-1693 ◽  
Author(s):  
Yi Hao ◽  
Wen Yuan ◽  
Chuanxin Ma ◽  
Jason C. White ◽  
Zetian Zhang ◽  
...  
Keyword(s):  
Virus Infection ◽  
Viral Infection ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Turnip Mosaic Virus ◽  
Model System ◽  
Crop Growth ◽  
Engineered Nanomaterials ◽  
Resistance To Viral Infection ◽  
Mosaic Virus Infection

Tobacco (Nicotiana benthamiana) and Turnip mosaic virus (TuMV) were used as a model system to investigate the potential of engineered nanomaterials (ENMs) for promoting crop growth and resistance to viral infection.

scholarly journals NbWRKY40 Positively Regulates the Response of Nicotiana benthamiana to Tomato Mosaic Virus via Salicylic Acid Signaling

2021 ◽  
Vol 11 ◽  
Author(s):  
Yaoyao Jiang ◽  
Weiran Zheng ◽  
Jing Li ◽  
Peng Liu ◽  
Kaili Zhong ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Mosaic Virus ◽  
Transcriptional Activation ◽  
Nicotiana Benthamiana ◽  
Yeast Cells ◽  
Tomato Mosaic Virus ◽  
Plant Responses ◽  
Mobility Shift ◽  
Localization Analysis ◽  
Electrophoretic Mobility Shift Assays

WRKY transcription factors play important roles in plants, including responses to stress; however, our understanding of the function of WRKY genes in plant responses to viral infection remains limited. In this study, we investigate the role of NbWRKY40 in Nicotiana benthamiana resistance to tomato mosaic virus (ToMV). NbWRKY40 is significantly downregulated by ToMV infection, and subcellular localization analysis indicates that NbWRKY40 is targeted to the nucleus. In addition, NbWRKY40 activates W-box-dependent transcription in plants and shows transcriptional activation in yeast cells. Overexpressing NbWRKY40 (OEWRKY40) inhibits ToMV infection, whereas NbWRKY40 silencing confers susceptibility. The level of salicylic acid (SA) is significantly higher in OEWRKY40 plants compared with that of wild-type plants. In addition, transcript levels of the SA-biosynthesis gene (ICS1) and SA-signaling genes (PR1b and PR2) are dramatically higher in OEWRKY40 plants than in the control but lower in NbWRKY40-silenced plants than in the control. Furthermore, electrophoretic mobility shift assays show that NbWRKY40 can bind the W-box element of ICS1. Callose staining reveals that the plasmodesmata is decreased in OEWRKY40 plants but increased in NbWRKY40-silenced plants. Exogenous application of SA also reduces viral accumulation in NbWRKY40-silenced plants infected with ToMV. RT-qPCR indicates that NbWRKY40 does not affect the replication of ToMV in protoplasts. Collectively, our findings suggest that NbWRKY40 likely regulates anti-ToMV resistance by regulating the expression of SA, resulting in the deposition of callose at the neck of plasmodesmata, which inhibits viral movement.

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