scholarly journals Comprehensive genomic survey, structural classification and expression analysis of C2H2-type zinc finger factor in wheat (Triticum aestivum L.)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yongliang Li ◽  
Aolong Sun ◽  
Qun Wu ◽  
Xiaoxiao Zou ◽  
Fenglin Chen ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Zinc Finger ◽  
Stress Responses ◽  
Molecular Breeding ◽  
Splice Variants ◽  
Triticum Aestivum L ◽  
Expression Data ◽  
Rt Pcr ◽  
Expression Pattern Analysis ◽  
Conserved Domain

Abstract Background The C2H2-type zinc finger proteins (C2H2-ZFPs) are one of major classes of transcription factors that play important roles in plant growth, development and stress responses. Limit information about the C2H2-ZF genes hinders the molecular breeding in bread wheat (Triticum aestivum). Results In this study, 457 C2H2-ZFP proteins (including 253 splice variants), which contain four types of conserved domain (named Q, M, Z, and D), could be further classified into ten subsets. They were identified to be distributed in 21 chromosomes in T. aestivum. Subset-specific motifs, like NPL-, SFP1-, DL- (EAR-like-motif), R-, PL-, L- and EK-, might make C2H2-ZFP diverse multifunction. Interestingly, NPL- and SFP1-box were firstly found to be located in C2H2-ZFP proteins. Synteny analyses showed that only 4 pairs of C2H2 family genes in T. aestivum, 65 genes in B. distachyon, 66 genes in A. tauschii, 68 genes in rice, 9 genes in Arabidopsis, were syntenic relationships respectively. It indicated that TaZFPs were closely related to genes in Poaceae. From the published transcriptome data, totally 198 of 204 TaC2H2-ZF genes have expression data. Among them, 25 TaC2H2-ZF genes were certificated to be significantly differentially expressed in 5 different organs and 15 different development stages by quantitative RT-PCR. The 18 TaC2H2-ZF genes were verified in response to heat, drought, and heat & drought stresses. According to expression pattern analysis, several TaZFPs, like Traes_5BL_D53A846BE.1, were not only highly expressed in L2DAAs, RTLS, RMS, but also endowed tolerance to drought and heat stresses, making them good candidates for molecular breeding. Conclusions This study systematically characterized the TaC2H2-ZFPs and their potential roles in T. aestivum. Our findings provide new insights into the C2H2-ZF genes in T. aestivum as well as a foundation for further studies on the roles of TaC2H2-ZF genes in T. aestivum molecular breeding.

scholarly journals Comprehensive genomic survey, structural classification and expression analysis of C2H2-type zinc finger factor in wheat (Triticum aestivum L.)

2021 ◽  
Author(s):  
Yongliang Li ◽  
Aolong Sun ◽  
Qun Wu ◽  
Xiaoxiao Zou ◽  
Fenglin Chen ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Zinc Finger ◽  
Stress Responses ◽  
Molecular Breeding ◽  
Pattern Analysis ◽  
Splice Variants ◽  
Expression Data ◽  
Rt Pcr ◽  
Expression Pattern Analysis ◽  
Conserved Domain

Abstract Background: The C2H2-type zinc finger proteins (C2H2-ZFPs) are one of major classes of transcription factors that play important roles during plant growth, development and stress responses. Limit information about the C2H2-ZF genes hinders the molecular breeding in bread wheat (Triticum aestivum). Results: In this study, 457 C2H2-ZFP proteins (including 253 splice variants), which contain four types of conserved domain (named Q, M, Z, and D), could be further classified into ten subsets. They were identified to be distributed on 21 chromosomes in T. aestivum. Subset-specific motifs, like NPL-, SFP1-, DL- (EAR-like-motif), R-, PL-, L- and EK-, might make C2H2-ZFP diverse multifunction. Interestingly, NPL- and SFP1-box were firstly found to be located in C2H2-ZFP proteins. Synteny analyses showed that only 4 pairs of C2H2 family genes in T. aestivum, 65 genes in B. distachyon, 66 genes in A. tauschii, 68 genes in rice, 9 genes in A. thaliana, were syntenic relationships respectively. It indicated that TaZFPs were closely related to genes in Poaceae. From the published transcriptome data, totally 198 of 204 TaC2H2-ZF genes have expression data. Among them, 25 TaC2H2-ZF genes were certificated to be significantly differentially expressed in 5 different organs and 15 different development stages by quantitative RT-PCR. The 18 TaC2H2-ZF genes were verified in response to heat, drought, and heat & drought stresses. According to expression pattern analysis, several TaZFPs, like Traes_5BL_D53A846BE.1, were not only highly expressed in L2DAAs, RTLS, RMS, but also endowed tolerance to drought and heat stresses, making them good candidates for molecular breeding. Conclusions: This study systematically characterized the TaC2H2-ZFPs and their potential roles in T. aestivum. Our findings provide new insights into the C2H2-ZF genes in T. aestivum as well as a foundation for further studies on the roles of TaC2H2-ZF genes in T. aestivum molecular breeding.

scholarly journals Comprehensive Genome-wide Identification, Characterization, and Expression Analysis of CCHC Zinc Finger Gene Family in Wheat (Triticum aestivum L.)

2022 ◽  
Author(s):  
Aolong Sun ◽  
Yongliang Li ◽  
Xiaoxiao Zou ◽  
Fenglin Chen ◽  
Ruqiong Cai ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Zinc Finger ◽  
Metal Ion ◽  
Triticum Aestivum L ◽  
Nucleic Acid Binding ◽  
Metal Ion Binding ◽  
Genome Wide ◽  
Gene Structures ◽  
Environmental Responses ◽  
Cchc Zinc Finger

Abstract Background: The CCHC zinc finger proteins (CCHC-ZFPs) are transcription factors that play versatile roles in plant growth, development, and responses to biotic/abiotic stress. However, little is known about the CCHC-ZF genes in bread wheat (Triticum aestivum), an important food crop.Results: In this study, 50 TaCCHC-ZF genes were identified and distributed unevenly on 21 wheat chromosomes. According to the phylogenetic features, the 50 TaCCHC-ZF genes were classified into eight groups with specific motifs and gene structures. 43 TaCCHC-ZF genes were identified as segmentally duplicated genes that formed 36 segmental duplication gene pairs. Additionally, the collinearity analyses between wheat and eight other representative plant species showed that wheat had closer phylogenetic relationships with monocots compared to dicots. A total of 636 cis-elements related to environmental stress and phytohormone responsiveness were identified in the promoter of TaCCHC-ZF genes. Moreover, GO enrichment results revealed that all 50 TaCCHC-ZF genes were annotated under metal ion binding and nucleic acid binding. 91 miRNA binding sites within the 34 TaCCHC-ZF genes were identified by miRNA targets analyses, indicating that the expression of TaCCHC-ZF genes could be regulated by the miRNAs. Based on published transcriptome data, 38 TaCCHC-ZF genes were identified as DEGs, and 15 TaCCHC-ZF genes among them were verified by qRT-PCR assays, which showed response to drought, heat, or simultaneous response of them.Conclusions: This study systematically explored the gene structures, evolutionary characteristics, and potential roles during environmental responses of TaCCHC-ZF genes, providing a foundation for further investigation and application of TaCCHC-ZF genes in the molecular breeding of T. aestivum.

scholarly journals Evidences of organic acids exudation in aluminium stress responses of two Madeiran wheat (Triticum aestivum L.) landraces

2019 ◽  
Vol 66 (4) ◽  
pp. 857-869 ◽  
Author(s):  
Marta Rodrigues ◽  
José Filipe T. Ganança ◽  
Emanuel M. da Silva ◽  
Teresa M. M. dos Santos ◽  
Jan J. Slaski ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Organic Acids ◽  
Stress Responses ◽  
Triticum Aestivum L

scholarly journals Genome-wide analysis of WRKY transcription factors in wheat (Triticum aestivum L.) and differential expression under water deficit condition

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3232 ◽  
Author(s):  
Pan Ning ◽  
Congcong Liu ◽  
Jingquan Kang ◽  
Jinyin Lv
Keyword(s):  
Triticum Aestivum ◽  
Water Deficit ◽  
Stress Responses ◽  
Segmental Duplication ◽  
Grain Filling ◽  
Triticum Aestivum L ◽  
Whole Wheat ◽  
Group I ◽  
Annotation Information ◽  
Specific Tissue

Background WRKY proteins, which comprise one of the largest transcription factor (TF) families in the plant kingdom, play crucial roles in plant development and stress responses. Despite several studies on WRKYs in wheat (Triticum aestivum L.), functional annotation information about wheat WRKYs is limited. Results Here, 171 TaWRKY TFs were identified from the whole wheat genome and compared with proteins from 19 other species representing nine major plant lineages. A phylogenetic analysis, coupled with gene structure analysis and motif determination, divided these TaWRKYs into seven subgroups (Group I, IIa–e, and III). Chromosomal location showed that most TaWRKY genes were enriched on four chromosomes, especially on chromosome 3B. In addition, 85 (49.7%) genes were either tandem (5) or segmental duplication (80), which suggested that though tandem duplication has contributed to the expansion of TaWRKY family, segmental duplication probably played a more pivotal role. Analysis of cis-acting elements revealed putative functions of WRKYs in wheat during development as well as under numerous biotic and abiotic stresses. Finally, the expression of TaWRKY genes in flag leaves, glumes, and lemmas under water-deficit condition were analyzed. Results showed that different TaWRKY genes preferentially express in specific tissue during the grain-filling stage. Conclusion Our results provide a more extensive insight on WRKY gene family in wheat, and also contribute to the screening of more candidate genes for further investigation on function characterization of WRKYs under various stresses.

scholarly journals Characterization of a common wheat (Triticum aestivum L.) TaSnRK2.7 gene involved in abiotic stress responses

2010 ◽  
Vol 62 (3) ◽  
pp. 975-988 ◽  
Author(s):  
Hongying Zhang ◽  
Xinguo Mao ◽  
Ruilian Jing ◽  
Xiaoping Chang ◽  
Huimin Xie
Keyword(s):  
Triticum Aestivum ◽  
Abiotic Stress ◽  
Common Wheat ◽  
Stress Responses ◽  
Triticum Aestivum L
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