Integrated ionomic and transcriptomic dissection reveals the core transporter genes responsive to varying cadmium abundances in allotetraploid rapeseed

Abstract Background Oilseed rape (B. napus L.) has great potential for phytoremediation of cadmium (Cd)-polluted soils due to its large plant biomass production and strong metal accumulation. Soil properties and the presence of other soluble compounds or ions, cause a heterogeneous distribution of Cd. Results The aim of our study was to reveal the differential responses of B. napus to different Cd abundances. Herein, we found that high Cd (50 μM) severely inhibited the growth of B. napus, which was not repressed by low Cd (0.50 μM) under hydroponic culture system. ICP-MS assays showed that the Cd2+ concentrations in both shoots and roots under 50 μM Cd were over 10 times higher than those under 0.50 μM Cd. Under low Cd, the concentrations of only shoot Ca2+/Mn2+ and root Mn2+ were obviously changed (both reduced); under high Cd, the concentrations of most cations assayed were significantly altered in both shoots and roots except root Ca2+ and Mg2+. High-throughput transcriptomic profiling revealed a total of 18,021 and 1408 differentially expressed genes under high Cd and low Cd conditions, respectively. The biological categories related to the biosynthesis of plant cell wall components and response to external stimulus were over-accumulated under low Cd, whereas the terms involving photosynthesis, nitrogen transport and response, and cellular metal ion homeostasis were highly enriched under high Cd. Differential expression of the transporters responsible for Cd uptake (NRAMPs), transport (IRTs and ZIPs), sequestration (HMAs, ABCs, and CAXs), and detoxification (MTPs, PCR, MTs, and PCSs), and some other essential nutrient transporters were investigated, and gene co-expression network analysis revealed the core members of these Cd transporters. Some Cd transporter genes, especially NRAMPs and IRTs, showed opposite responsive patterns between high Cd and low Cd conditions. Conclusions Our findings would enrich our understanding of the interaction between essential nutrients and Cd, and might also provide suitable gene resources and important implications for the genetic improvement of plant Cd accumulation and resistance through molecular engineering of these core genes under varying Cd abundances in soils.

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Zinc limitation in Klebsiella pneumoniae profiled by quantitative proteomics influences transcriptional regulation and cation transporter-associated capsule production

BMC Microbiology ◽

10.1186/s12866-021-02091-8 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

A. Sukumaran ◽

S. Pladwig ◽

J. Geddes-McAlister

Keyword(s):

Klebsiella Pneumoniae ◽

Metal Ion ◽

Ion Homeostasis ◽

Cellular Protein ◽

Pcr Analysis ◽

Phenotypic Analysis ◽

Metal Ion Homeostasis ◽

Capsule Production ◽

The Impact

Abstract Background Microbial organisms encounter a variety of environmental conditions, including changes to metal ion availability. Metal ions play an important role in many biological processes for growth and survival. As such, microbes alter their cellular protein levels and secretion patterns in adaptation to a changing environment. This study focuses on Klebsiella pneumoniae, an opportunistic bacterium responsible for nosocomial infections. By using K. pneumoniae, we aim to determine how a nutrient-limited environment (e.g., zinc depletion) modulates the cellular proteome and secretome of the bacterium. By testing virulence in vitro, we provide novel insight into bacterial responses to limited environments in the presence of the host. Results Analysis of intra- and extracellular changes identified 2380 proteins from the total cellular proteome (cell pellet) and 246 secreted proteins (supernatant). Specifically, HutC, a repressor of the histidine utilization operon, showed significantly increased abundance under zinc-replete conditions, which coincided with an expected reduction in expression of genes within the hut operon from our validating qRT-PCR analysis. Additionally, we characterized a putative cation transport regulator, ChaB that showed significantly higher abundance under zinc-replete vs. -limited conditions, suggesting a role in metal ion homeostasis. Phenotypic analysis of a chaB deletion strain demonstrated a reduction in capsule production, zinc-dependent growth and ion utilization, and reduced virulence when compared to the wild-type strain. Conclusions This is first study to comprehensively profile the impact of zinc availability on the proteome and secretome of K. pneumoniae and uncover a novel connection between zinc transport and capsule production in the bacterial system.

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Metallothionein: A Multifunctional Protein from Toxicity to Cancer

The International Journal of Biological Markers ◽

10.1177/172460080301800302 ◽

2003 ◽

Vol 18 (3) ◽

pp. 162-169 ◽

Cited By ~ 46

Author(s):

S.E. Theocharis ◽

A.P. Margeli ◽

A. Koutselinis

Keyword(s):

Cell Proliferation ◽

Metal Ion ◽

Defense Mechanisms ◽

Ion Homeostasis ◽

Low Molecular Weight ◽

Multifunctional Protein ◽

Metal Ion Homeostasis ◽

Potential Marker ◽

Proliferation And Apoptosis ◽

Carcinogenic Process

The metallothionein (MT) family is a class of low molecular weight, intracellular and cysteine-rich proteins presenting high affinity for metal ions. Although the members of this family were discovered nearly 40 years ago, their functional significance remains obscure. Four major MT isoforms, MT-1, MT-2, MT-3 and MT-4, have been identified in mammals. MTs are involved in many pathophysiological processes such as metal ion homeostasis and detoxification, protection against oxidative damage, cell proliferation and apoptosis, chemoresistance and radiotherapy resistance. MT isoforms have been shown to be involved in several aspects of the carcinogenic process, cancer development and progression. MT expression has been implicated as a transient response to any form of stress or injury providing cytoprotective action. Although MT participates in the carcinogenic process, its use as a potential marker of tumor differentiation or cell proliferation, or as a predictor of poor prognosis remains unclear. In the present review the involvement of MT in defense mechanisms to toxicity and in carcinogenicity is discussed.

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Metallochaperones & Metal Ion Homeostasis

Encyclopedia of Inorganic Chemistry ◽

10.1002/0470862106.ia276 ◽

2006 ◽

Author(s):

Valeria C. Culotta

Keyword(s):

Metal Ion ◽

Ion Homeostasis ◽

Metal Ion Homeostasis

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Metal Ion Homeostasis

Reference Module in Chemistry, Molecular Sciences and Chemical Engineering ◽

10.1016/b978-0-12-409547-2.14675-x ◽

2019 ◽

Author(s):

Jiefei Wang ◽

Daiana A. Capdevila ◽

David P. Giedroc

Keyword(s):

Metal Ion ◽

Ion Homeostasis ◽

Metal Ion Homeostasis

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The novel ECF56 SigG1-RsfG system modulates morphological differentiation and metal-ion homeostasis in Streptomyces tsukubaensis

Scientific Reports ◽

10.1038/s41598-020-78520-x ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Rute Oliveira ◽

Matthew J. Bush ◽

Sílvia Pires ◽

Govind Chandra ◽

Delia Casas-Pastor ◽

...

Keyword(s):

Metal Ion ◽

Ion Homeostasis ◽

Morphological Differentiation ◽

The Novel ◽

Copper Trafficking ◽

Streptomyces Tsukubaensis ◽

Metal Ion Homeostasis ◽

Σ Factor ◽

Domain Organisation

AbstractExtracytoplasmic function (ECF) sigma factors are key transcriptional regulators that prokaryotes have evolved to respond to environmental challenges. Streptomyces tsukubaensis harbours 42 ECFs to reprogram stress-responsive gene expression. Among them, SigG1 features a minimal conserved ECF σ2–σ4 architecture and an additional C-terminal extension that encodes a SnoaL_2 domain, which is characteristic for ECF σ factors of group ECF56. Although proteins with such domain organisation are widely found among Actinobacteria, the functional role of ECFs with a fused SnoaL_2 domain remains unknown. Our results show that in addition to predicted self-regulatory intramolecular amino acid interactions between the SnoaL_2 domain and the ECF core, SigG1 activity is controlled by the cognate anti-sigma protein RsfG, encoded by a co-transcribed sigG1-neighbouring gene. Characterisation of ∆sigG1 and ∆rsfG strains combined with RNA-seq and ChIP-seq experiments, suggests the involvement of SigG1 in the morphological differentiation programme of S. tsukubaensis. SigG1 regulates the expression of alanine dehydrogenase, ald and the WhiB-like regulator, wblC required for differentiation, in addition to iron and copper trafficking systems. Overall, our work establishes a model in which the activity of a σ factor of group ECF56, regulates morphogenesis and metal-ions homeostasis during development to ensure the timely progression of multicellular differentiation.

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Metal Ion Homeostasis Mediated by NRAMP Transporters in Plant Cells - Focused on Increased Resistance to Iron and Cadmium Ion

Crosstalk and Integration of Membrane Trafficking Pathways ◽

10.5772/30905 ◽

2012 ◽

Cited By ~ 5

Author(s):

Toshio Sano ◽

Toshihiro Yoshihara ◽

Koichi Handa ◽

Masa H. ◽

Toshiyuki Nagata ◽

...

Keyword(s):

Metal Ion ◽

Ion Homeostasis ◽

Plant Cells ◽

Cadmium Ion ◽

Metal Ion Homeostasis

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The Balance between Life and Death of Cells: Roles of Metallothioneins

Biomarker Insights ◽

10.1177/117727190600100016 ◽

2006 ◽

Vol 1 ◽

pp. 117727190600100 ◽

Cited By ~ 5

Author(s):

Allan Evald Nielsen ◽

Adam Bohr ◽

Milena Penkowa

Keyword(s):

Metal Ion ◽

Molecular Mechanisms ◽

Energy Levels ◽

Ion Homeostasis ◽

Cellular Survival ◽

Life And Death ◽

Cellular Energy ◽

Metal Ion Homeostasis ◽

Pathological Conditions ◽

Cysteine Rich Protein

Metallothionein (MT) is a highly conserved, low-molecular-weight, cysteine-rich protein that occurs in 4 isoforms (MT-I to MT-IV), of which MT-I+II are the major and best characterized proteins. This review will focus on mammalian MT-I+II and their functional impact upon cellular survival and death, as seen in two rather contrasting pathological conditions: Neurodegeneration and neoplasms. MT-I+II have analogous functions including: 1) Antioxidant scavenging of reactive oxygen species (ROS); 2) Cytoprotection against degeneration and apoptosis; 3) Stimulation of cell growth and repair including angiogenesis/revascularization, activation of stem/progenitor cells, and neuroregeneration. Thereby, MT-I+II mediate neuroprotection, CNS restoration and clinical recovery during neurodegenerative disorders. Due to the promotion of cell survival, increased MT-I+II levels have been associated with poor tumor prognosis, although the data are less clear and direct causative roles of MT-I+II in oncogenesis remain to be identified. The MT-I+II molecular mechanisms of actions are not fully elucidated. However, their role in metal ion homeostasis might be fundamental in controlling Zn-dependent transcription factors, protein synthesis, cellular energy levels/metabolism and cell redox state. Here, the neuroprotective and regenerative functions of MT-I+II are reviewed, and the presumed link to oncogenesis is critically perused.

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The SloR/Dlg Metalloregulator Modulates Streptococcus mutans Virulence Gene Expression

Journal of Bacteriology ◽

10.1128/jb.00155-06 ◽

2006 ◽

Vol 188 (14) ◽

pp. 5033-5044 ◽

Cited By ~ 50

Author(s):

Elizabeth Rolerson ◽

Adam Swick ◽

Lindsay Newlon ◽

Cameron Palmer ◽

Yong Pan ◽

...

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Streptococcus Mutans ◽

Metal Ion ◽

Ion Homeostasis ◽

Virulence Gene ◽

Wild Type ◽

Genetic Competence ◽

Virulence Gene Expression ◽

Metal Ion Homeostasis

ABSTRACT Metal ion availability in the human oral cavity plays a putative role in Streptococcus mutans virulence gene expression and in appropriate formation of the plaque biofilm. In this report, we present evidence that supports such a role for the DtxR-like SloR metalloregulator (called Dlg in our previous publications) in this oral pathogen. Specifically, the results of gel mobility shift assays revealed the sloABC, sloR, comDE, ropA, sod, and spaP promoters as targets of SloR binding. We confirmed differential expression of these genes in a GMS584 SloR-deficient mutant versus the UA159 wild-type progenitor by real-time semiquantitative reverse transcriptase PCR experiments. The results of additional expression studies support a role for SloR in S. mutans control of glucosyltransferases, glucan binding proteins, and genes relevant to antibiotic resistance. Phenotypic analysis of GMS584 revealed that it forms aberrant biofilms on an abiotic surface, is compromised for genetic competence, and demonstrates heightened incorporation of iron and manganese as well as resistance to oxidative stress compared to the wild type. Taken together, these findings support a role for SloR in S. mutans adherence, biofilm formation, genetic competence, metal ion homeostasis, oxidative stress tolerance, and antibiotic gene regulation, all of which contribute to S. mutans-induced disease.

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