Prevalence of Escherichia coli O157:H7 in beef cattle at slaughter and beef carcasses at retail shops in Ethiopia

Two hundred steers and heifers from a large feedyard (65,000-head capacity) were used to determine the prevalence levels of enterohemorrhagic Escherichia coli O157 (EHEC O157) and Salmonella spp. prior to and after shipping to a commercial packing facility. Two samples, a ventral midline hide swab and a fecal sample, were aseptically collected from each animal 2 weeks prior to the date of transportation and at the packing plant immediately after exsanguination. Samples were collected from all trailers (n = 46) before animals were loaded for transport to the packing facility. The average prevalence levels of EHEC O157 on hides (18%) and in feces (9.5%) at the feedyard decreased (P > 0.05) at the packing plant to 4.5 and 5.5%, respectively. The average prevalence levels of Salmonella spp. on hides (6%) and in feces (18%) at the feedyard increased to 89 and 46%, respectively, upon arrival at the packing plant. Average prevalence levels for EHEC O157 and Salmonella spp. on the trailers were 5.43 and 59%, respectively. The results of this study demonstrate that transportation may be a potential stressor for cattle, as evidenced by the increased shedding of Salmonella spp.

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Reduced intestinal colonization of adult beef cattle by Escherichia coli O157:H7 tir deletion and nalidixic-acid-resistant mutants lacking flagellar expression

Veterinary Microbiology ◽

10.1016/j.vetmic.2007.06.009 ◽

2007 ◽

Vol 125 (3-4) ◽

pp. 381-386 ◽

Cited By ~ 15

Author(s):

Gustavo Bretschneider ◽

Emil M. Berberov ◽

Rodney A. Moxley

Keyword(s):

Escherichia Coli ◽

Beef Cattle ◽

Nalidixic Acid ◽

Escherichia Coli O157 ◽

Intestinal Colonization ◽

Resistant Mutants

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Longitudinal Study of Escherichia coli O157:H7 in a Beef Cattle Feedlot and Role of High-Level Shedders in Hide Contamination

Applied and Environmental Microbiology ◽

10.1128/aem.00081-09 ◽

2009 ◽

Vol 75 (20) ◽

pp. 6515-6523 ◽

Cited By ~ 93

Author(s):

Terrance M. Arthur ◽

James E. Keen ◽

Joseph M. Bosilevac ◽

Dayna M. Brichta-Harhay ◽

Norasak Kalchayanand ◽

...

Keyword(s):

Escherichia Coli ◽

Longitudinal Study ◽

Beef Cattle ◽

Intervention Studies ◽

High Density ◽

Escherichia Coli O157 ◽

E Coli ◽

High Level ◽

Cattle Feedlot

ABSTRACT The objectives of the study described here were (i) to investigate the dynamics of Escherichia coli O157:H7 fecal and hide prevalence over a 9-month period in a feedlot setting and (ii) to determine how animals shedding E. coli O157:H7 at high levels affect the prevalence and levels of E. coli O157:H7 on the hides of other animals in the same pen. Cattle (n = 319) were distributed in 10 adjacent pens, and fecal and hide levels of E. coli O157:H7 were monitored. When the fecal pen prevalence exceeded 20%, the hide pen prevalence was usually (25 of 27 pens) greater than 80%. Sixteen of 19 (84.2%) supershedder (>104 CFU/g) pens had a fecal prevalence greater than 20%. Significant associations with hide and high-level hide (≥40 CFU/100 cm2) contamination were identified for (i) a fecal prevalence greater than 20%, (ii) the presence of one or more high-density shedders (≥200 CFU/g) in a pen, and (iii) the presence of one or more supershedders in a pen. The results presented here suggest that the E. coli O157:H7 fecal prevalence should be reduced below 20% and the levels of shedding should be kept below 200 CFU/g to minimize the contamination of cattle hides. Also, large and unpredictable fluctuations within and between pens in both fecal and hide prevalence of E. coli O157:H7 were detected and should be used as a guide when preharvest studies, particularly preharvest intervention studies, are designed.

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Virulence Characterization and Molecular Subtyping of Typical and Atypical Escherichia coli O157:H7 and O157:H(−) Isolated from Fecal Samples and Beef Carcasses in Mexico

Journal of Food Protection ◽

10.4315/0362-028x.jfp-14-348 ◽

2015 ◽

Vol 78 (2) ◽

pp. 264-272 ◽

Cited By ~ 4

Author(s):

CLAUDIA NARVÁEZ-BRAVO ◽

ALEJANDRO ECHEVERRY ◽

MARKUS F. MILLER ◽

ARGENIS RODAS-GONZÁLEZ ◽

M. TODD BRASHEARS ◽

...

Keyword(s):

Escherichia Coli ◽

Virulence Genes ◽

Immunomagnetic Separation ◽

Latex Agglutination ◽

Escherichia Coli O157 ◽

High Genetic Diversity ◽

E Coli ◽

System A ◽

Metabolic Genes ◽

Beef Carcasses

The objective of the study was to characterize virulence genes and subtype Escherichia coli O157:H7 and O157:H(−) isolates obtained from a vertically integrated feedlot slaughter plant in Mexico. A total of 1,695 samples were collected from feedlots, holding pens, colon contents, hides, and carcasses. E. coli O157:H7 detection and confirmation was carried out using conventional microbiology techniques, immunomagnetic separation, latex agglutination, and the BAX system. A total of 97 E. coli O157 strains were recovered and screened for key virulence and metabolic genes using multiplex and conventional PCR. Eighty-eight (91.72%) of the strains carried stx2, eae, and ehxA genes. Ten isolates (8.25%) were atypical sorbitol-fermenting strains, and nine were negative for the flicH7 gene and lacked eae, stx1, stx2, and ehxA. One sorbitol-positive strain carried stx2, eae, tir, toxB, and iha genes but was negative for stx1 and ehxA. Pulsed-field gel electrophoresis (PFGE) analysis yielded 49 different PFGE subtypes, showing a high genetic diversity; however, the majority of the typical isolates were closely related (80 to 90% cutoff). Atypical O157 isolates were not closely related within them or to typical E. coli O157:H7 isolates. Identical PFGE subtypes were found in samples obtained from colon contents, feedlots, holding pens, and carcasses. Isolation of a sorbitol-fermenting E. coli O157 positive for a number of virulence genes is a novel finding in Mexico. These data showed that genetically similar strains of E. coli O157:H7 can be found at various stages of beef production and highlights the importance of preventing cross-contamination at the pre- and postharvest stages of processing.

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Identifikasi Bakteri Escherichia coli O157:H7 dalam Daging Sapi yang Berasal dari Rumah Potong Hewan Lubuk Buaya

Jurnal Kesehatan Andalas ◽

10.25077/jka.v5i3.586 ◽

2016 ◽

Vol 5 (3) ◽

Cited By ~ 1

Author(s):

Rizki Muhammad Rananda ◽

Aziz Djamal ◽

Julizar Julizar

Keyword(s):

Escherichia Coli ◽

Beef Cattle ◽

Hemolytic Uremic Syndrome ◽

Human Infection ◽

Escherichia Coli O157 ◽

Foodborne Disease ◽

Bowel Necrosis ◽

Slaughter House ◽

Uremic Syndrome ◽

Cooked Meat

AbstrakEscherichia coli O157:H7 adalah penyebab penting foodborne disease di banyak negara. Infeksi pada manusia oleh bakteri Escherichia coli O157:H7 sering dihubungkan dengan konsumsi daging sapi yang kurang matang dan dapat menyebabkan diare berdarah, nekrosis jaringan usus, hemorraghic colitis (HC) dan hemolytic uremic syndrome (HUS). Tujuan penelitian ini ialah mengidentifikasi bakteri Escherichia coli O157:H7 dalam daging sapi yang dipotong di RPH Lubuk Buaya. Penelitian deskriptif ini telah dilaksanakan dari Januari 2012 sampai Juni 2012. Untuk mengidentifikasi bakteri Escherichia coli O157:H7 digunakan medium CHROMagar O157. Tujuh dari sepuluh sampel yang diteliti menunjukkan kontaminasi bakteri Escherichia coli O157:H7. Disarankan pada masyarakat untuk memakan daging yang telah dimasak sampai benar-benar matang.Kata kunci: Escherichia coli O157:H7, daging sapi, rumah potong hewan AbstractEscherichia coli O157:H7 is an important cause of foodborne disease in many countries. Human infection by Escherichia coli O157:H7 is frequently associated with consumption of undercooked beef. This infection can cause bloody diarrhea, bowel necrosis, hemorraghic colitis (HC), and hemolytic uremic syndrome (HUS). The objective of this study was to identify Escherichia coli O157:H7 in beef cattle at Lubuk Buaya slaughter house. This descriptive study was conducted at Lubuk Buaya slaughter house. For identification of Escherichia coli O157:H7, samples were plated onto CHROMagar O157. Seven of ten samples were positively contaminated by Escherichia coli O157:H7. This research suggests to all community only to eat well-cooked meat. Keywords: Escherichia coli O157:H7, beef cattle, slaughter house

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Effects of Low-Dose, Low-Penetration Electron Beam Irradiation of Chilled Beef Carcass Surface Cuts on Escherichia coli O157:H7 and Meat Quality†

Journal of Food Protection ◽

10.4315/0362-028x-68.4.666 ◽

2005 ◽

Vol 68 (4) ◽

pp. 666-672 ◽

Cited By ~ 33

Author(s):

TERRANCE M. ARTHUR ◽

TOMMY L. WHEELER ◽

STEVEN D. SHACKELFORD ◽

JOSEPH M. BOSILEVAC ◽

XIANGWU NOU ◽

...

Keyword(s):

Escherichia Coli ◽

Electron Beam ◽

Low Dose ◽

Ground Beef ◽

Escherichia Coli O157 ◽

Beam Irradiation ◽

Beam Radiation ◽

Beef Carcasses ◽

Antimicrobial Intervention ◽

Potential Use

Low-dose, low-penetration electron beam (E-beam) irradiation was evaluated for potential use as an antimicrobial intervention on beef carcasses during processing. The objectives of this study were (i) to assess the efficacy of E-beam irradiation to reduce concentrations of Escherichia coli O157:H7 on a large beef surface and (ii) to evaluate the effect of the treatment on the sensory properties of the product. A 1-kGy dose of E-beam radiation reduced E. coli O157:H7 inoculated onto sections of cutaneous trunci at least 4 log CFU/cm2. In assessing organoleptic impact, flank steak was used as the model muscle. Flank steaks with various levels of penetration by radiation (5, 10, 25, 50, and 75%) were evaluated. None of the flank steak sensory attributes were affected (P > 0.05) by any penetration treatment. Ground beef formulations consisting of 100, 50, 25, 10, 5, and 0% surface-irradiated beef were tested. A trained sensory panel did not detect any difference between the control (0%) and either the 5 or 10% treatments. These results suggest that if chilled carcasses were subjected to low-dose E-beam irradiation, aroma and flavor of ground beef would not be impacted. The data presented here indicate that low-dose, low-penetration E-beam irradiation has potential use as an antimicrobial intervention on beef carcasses during processing and minimally impacts the organoleptic qualities of the treated beef products.

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Faculty Opinions recommendation of Occurrence of Escherichia coli O157:H7 in Pest Flies Captured in Leafy Greens Plots Grown Near a Beef Cattle Feedlot.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.736555876.793564833 ◽

2019 ◽

Author(s):

Marilyn Roberts

Keyword(s):

Escherichia Coli ◽

Beef Cattle ◽

Escherichia Coli O157 ◽

Leafy Greens ◽

Cattle Feedlot

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