Anti-allergic activities of Umbelliferone against histamine- and Picryl chloride-induced ear edema by targeting Nrf2/iNOS signaling in mice

Abstract Background The current study was aimed to investigate the anti-allergic activities of the Umbelliferone (UMB) against the acute Histamine and chronic Picryl chloride (PiCl)-induced allergy in mice. UMB is a coumarin derivative (isolated from Angelica decursiva) found in various parts of the plants such as flowers, roots and, stems isolated from the plants of Umbelliferae family. Methods The UMB (1, 10, 50 mg/kg) was administered intraperitoneally (i.p) half an h before or 2 h after the induction of allergic ear edema. The acute ear edema was induced by histamine (intradermally, i.d), while the chronic ear edema was induced by painting the PiCl (sensitized with the toluene) on the ear. The antioxidants and oxidative stress markers were assessed. The histological changes were assessed using Hematoxylin and eosin (H and E) and giemsa staining. The immunohistochemistry studies were performed to assess the expression of the nuclear factor erythroid 2-related factor 2 (Nrf2) and inducible nitric oxide synthase (iNOS). The data was analyzed using one-way ANOVA tests followed by Tukey’s test with p < 0.05 was chosen as criteria for statistical significance. Results UMB treatment markedly reduced the allergic ear edema and ear weight compared to the negative control. Furthermore, the UMB attenuated the oxidative stress markers, while induced the antioxidants enzymes. Similarly, the UMB treatment significantly attenuated the serum immunoglobulin E (IgE) level. The UMB treatment markedly improved the histological parameters using H and E staining and Giemsa staining. The UMB administration induced the Nrf2 expression, while attenuated the iNOS expression. Furthermore, the computational analysis was performed to assess the interaction of the UMB with the various protein targets and to determine the mechanism of interaction with the target proteins. Conclusion In conclusion, the UMB treatment significantly alleviated the allergic symptoms, attenuating the oxidative stress, improved the histological features using in vivo and computational approaches.

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Evaluation of clay/poly (l-lactide) microcomposites as anticancer drug, 6-mercaptopurine reservoir through in vitro cytotoxicity, oxidative stress markers and in vivo pharmacokinetics

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2013.07.008 ◽

2013 ◽

Vol 112 ◽

pp. 400-407 ◽

Cited By ~ 12

Author(s):

Bhavesh D. Kevadiya ◽

Shiva Shankaran Chettiar ◽

Shalini Rajkumar ◽

Hari C. Bajaj ◽

Kalpeshgiri A. Gosai ◽

...

Keyword(s):

Oxidative Stress ◽

Anticancer Drug ◽

In Vitro Cytotoxicity ◽

Oxidative Stress Markers ◽

Stress Markers ◽

In Vivo Pharmacokinetics

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Ochratoxin A presence in Cabernet Sauvignon wine changes antioxidant activity in vitro and oxidative stress markers in vivo

Food Additives & Contaminants Part A ◽

10.1080/19440049.2020.1802067 ◽

2020 ◽

Vol 37 (10) ◽

pp. 1755-1764

Author(s):

Luana Schmidt ◽

Natália de Vargas Heck ◽

Ingrid Ferreira ◽

Gabriela Göethel ◽

Sabrina Somacal ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Ochratoxin A ◽

Cabernet Sauvignon ◽

Oxidative Stress Markers ◽

Stress Markers ◽

And Oxidative Stress

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Does oxidative stress shorten telomeres?

Biology Letters ◽

10.1098/rsbl.2017.0164 ◽

2017 ◽

Vol 13 (5) ◽

pp. 20170164 ◽

Cited By ~ 49

Author(s):

Jelle J. Boonekamp ◽

Christina Bauch ◽

Ellis Mulder ◽

Simon Verhulst

Keyword(s):

Oxidative Stress ◽

Animal Cell ◽

High Rate ◽

Oxidative Stress Markers ◽

Base Pairs ◽

Telomere Shortening ◽

Telomere Attrition ◽

Stress Markers ◽

Corvus Monedula

Oxidative stress shortens telomeres in cell culture, but whether oxidative stress explains variation in telomere shortening in vivo at physiological oxidative stress levels is not well known. We therefore tested for correlations between six oxidative stress markers and telomere attrition in nestling birds (jackdaws Corvus monedula ) that show a high rate of telomere attrition in early life. Telomere attrition was measured between ages 5 and 30 days, and was highly variable (average telomere loss: 323 bp, CV = 45%). Oxidative stress markers were measured in blood at age 20 days and included markers of oxidative damage (TBARS, dROMs and GSSG) and markers of antioxidant protection (GSH, redox state, uric acid). Variation in telomere attrition was not significantly related to these oxidative stress markers (| r | ≤ 0.08, n = 87). This finding raises the question whether oxidative stress accelerates telomere attrition in vivo . The accumulation of telomere attrition over time depends both on the number of cell divisions and on the number of base pairs lost per DNA replication and, based on our findings, we suggest that in a growing animal cell proliferation, dynamics may be more important for explaining variation in telomere attrition than oxidative stress.

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Vanadium and cadmium in vivo effects in teleost cardiac muscle: Metal accumulation and oxidative stress markers

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology ◽

10.1016/j.cbpc.2007.09.003 ◽

2008 ◽

Vol 147 (2) ◽

pp. 168-178 ◽

Cited By ~ 27

Author(s):

S.S. Soares ◽

H. Martins ◽

C. Gutiérrez-Merino ◽

M. Aureliano

Keyword(s):

Oxidative Stress ◽

Cardiac Muscle ◽

Metal Accumulation ◽

Oxidative Stress Markers ◽

Stress Markers ◽

In Vivo Effects ◽

And Oxidative Stress

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Berries and oxidative stress markers: an overview of human intervention studies

Food & Function ◽

10.1039/c5fo00657k ◽

2015 ◽

Vol 6 (9) ◽

pp. 2890-2917 ◽

Cited By ~ 43

Author(s):

Cristian Del Bo’ ◽

Daniela Martini ◽

Marisa Porrini ◽

Dorothy Klimis-Zacas ◽

Patrizia Riso

Keyword(s):

Oxidative Stress ◽

Intervention Studies ◽

Human Intervention ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Intervention Trials ◽

And Oxidative Stress

Severalin vitroandin vivostudies have demonstrated that polyphenol-rich berries may counteract oxidative stress. In this review, we summarized the main finding from human intervention trials on the role of berries in the modulation of markers of oxidative lipid, protein and DNA damage.

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The effect of the ALAnerv nutritional supplement on some oxidative stress markers in postacute stroke patients undergoing rehabilitation

Applied Physiology Nutrition and Metabolism ◽

10.1139/apnm-2012-0436 ◽

2013 ◽

Vol 38 (6) ◽

pp. 613-620 ◽

Cited By ~ 4

Author(s):

Eliza Oprea ◽

Mihai Berteanu ◽

Delia Cintezã ◽

Bogdan Nicolae Manolescu

Keyword(s):

Oxidative Stress ◽

Oxidized Ldl ◽

Statistical Significance ◽

Lipid Hydroperoxide ◽

Nutritional Supplement ◽

Protein Carbonyls ◽

Oxidative Stress Markers ◽

Stroke Patients ◽

Stress Markers ◽

Ldl Particles

Stroke is a pathologic condition associated with redox imbalance. This pilot study was designed to evaluate the effect of the consumption of the nutritional supplement ALAnerv on some oxidative stress markers in postacute stroke patients undergoing rehabilitation. To achieve this goal, we assigned 28 patients to 2 study groups: (−)ALA and (+)ALA. Patients in both groups participated in the same rehabilitation program and received comparable standard medications; however, patients in the (+)ALA group received ALAnerv for 2 weeks (2 pills per day). We assessed total and nonproteic thiols, protein carbonyls, ceruloplasmin, oxidized low-density lipoprotein (LDL) particles, lipid hydroperoxide concentrations, gamma-glutamyl transpeptidase activity, and total antioxidant capacity. Regression analysis indicated that supplementation with ALAnerv was responsible for the significant decrease in glucose (p = 0.002) and oxidized LDL particles (p < 0.001) during the study period. For both parameters, the variation in the percent of concentration between the 2 groups during the study period reached statistical significance (p = 0.012 and p < 0.001, respectively). Moreover, Barthel Index values at discharge were significantly influenced by ALAnerv treatment. These preliminary results indicate that ALAnerv might be helpful because it rapidly corrects plasma fasting glucose and corrects serum oxidized LDL particle concentrations, suggesting the need for longer treatment with 2 pills or more per day.

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Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/6428498 ◽

2020 ◽

Vol 2020 ◽

pp. 1-13

Author(s):

Zhang Yifan ◽

Ning Benxiang ◽

Xu Zheng ◽

Xu Luwei ◽

Zhou Liuhua ◽

...

Keyword(s):

Oxidative Stress ◽

Kidney Injury ◽

Oxidative Stress Markers ◽

Stress Injury ◽

Stress Markers ◽

Oxidative Stress Injury ◽

Calcium Crystals ◽

And Oxidative Stress

Objective. To investigate the role of inflammatory reactions and oxidative stress injury in the mechanisms of ceftriaxone calcium crystal-induced acute kidney injury (AKI) both in vivo and in vitro. Methods. Male Sprague Dawley rats were randomly divided into five groups of ten each according to different concentrations of ceftriaxone and calcium. Based on the levels of serum creatinine (Scr) and blood urea nitrogen (BUN), the AKI group was chosen for the subsequent experiments. Kidney histological examination and immunohistochemistry were performed. The expression of NLRP3 and IL-1β protein and the concentrations of oxidative stress markers such as ROS, MDA, and H2O2 in kidney tissues were estimated. In parallel, HK-2 human renal proximal tubule cells were exposed to ceftriaxone calcium crystals. The mRNA expression levels of NLRP3 and IL-1β and the concentrations of oxidative stress markers were evaluated. Finally, cell viability and rat survival were also assessed. Results. The results showed that significantly increased Scr and BUN levels, consistent with morphological changes and kidney stones, were found in the rats that received the highest concentration of ceftriaxone (1000 mg/kg) combined with calcium (800 mg/kg). The activation of the NLRP3 inflammasome axis and the marked elevation of MDA, H2O2, and ROS levels were observed both in vivo and in vitro. High expression of Nrf2, HO-1, and NQO1 was also documented. In addition, cell apoptosis and rat mortality were promoted by ceftriaxone calcium crystals. Conclusions. Notably, we found that ceftriaxone-induced urolithiasis was associated with a high risk of AKI and NLRP3-mediated inflammasome and oxidative stress injury were of major importance in the pathogenesis.

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