Evaluation of clay/poly (l-lactide) microcomposites as anticancer drug, 6-mercaptopurine reservoir through in vitro cytotoxicity, oxidative stress markers and in vivo pharmacokinetics

Severalin vitroandin vivostudies have demonstrated that polyphenol-rich berries may counteract oxidative stress. In this review, we summarized the main finding from human intervention trials on the role of berries in the modulation of markers of oxidative lipid, protein and DNA damage.

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Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/6428498 ◽

2020 ◽

Vol 2020 ◽

pp. 1-13

Author(s):

Zhang Yifan ◽

Ning Benxiang ◽

Xu Zheng ◽

Xu Luwei ◽

Zhou Liuhua ◽

...

Keyword(s):

Oxidative Stress ◽

Kidney Injury ◽

Oxidative Stress Markers ◽

Stress Injury ◽

Stress Markers ◽

Oxidative Stress Injury ◽

Calcium Crystals ◽

And Oxidative Stress

Objective. To investigate the role of inflammatory reactions and oxidative stress injury in the mechanisms of ceftriaxone calcium crystal-induced acute kidney injury (AKI) both in vivo and in vitro. Methods. Male Sprague Dawley rats were randomly divided into five groups of ten each according to different concentrations of ceftriaxone and calcium. Based on the levels of serum creatinine (Scr) and blood urea nitrogen (BUN), the AKI group was chosen for the subsequent experiments. Kidney histological examination and immunohistochemistry were performed. The expression of NLRP3 and IL-1β protein and the concentrations of oxidative stress markers such as ROS, MDA, and H2O2 in kidney tissues were estimated. In parallel, HK-2 human renal proximal tubule cells were exposed to ceftriaxone calcium crystals. The mRNA expression levels of NLRP3 and IL-1β and the concentrations of oxidative stress markers were evaluated. Finally, cell viability and rat survival were also assessed. Results. The results showed that significantly increased Scr and BUN levels, consistent with morphological changes and kidney stones, were found in the rats that received the highest concentration of ceftriaxone (1000 mg/kg) combined with calcium (800 mg/kg). The activation of the NLRP3 inflammasome axis and the marked elevation of MDA, H2O2, and ROS levels were observed both in vivo and in vitro. High expression of Nrf2, HO-1, and NQO1 was also documented. In addition, cell apoptosis and rat mortality were promoted by ceftriaxone calcium crystals. Conclusions. Notably, we found that ceftriaxone-induced urolithiasis was associated with a high risk of AKI and NLRP3-mediated inflammasome and oxidative stress injury were of major importance in the pathogenesis.

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The effects of oral Cardax™ (disodium disuccinate astaxanthin) on multiple independent oxidative stress markers in a mouse peritoneal inflammation model: influence on 5-lipoxygenase in vitro and in vivo

Life Sciences ◽

10.1016/j.lfs.2005.12.052 ◽

2006 ◽

Vol 79 (2) ◽

pp. 162-174 ◽

Cited By ~ 11

Author(s):

Samuel F. Lockwood ◽

Marc S. Penn ◽

Stanley L. Hazen ◽

Zsolt Bikádi ◽

Ferenc Zsila

Keyword(s):

Oxidative Stress ◽

Oxidative Stress Markers ◽

Peritoneal Inflammation ◽

Inflammation Model ◽

Stress Markers ◽

Disodium Disuccinate Astaxanthin

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Measurement of Oxidative Stress Markers In Vitro Using Commercially Available Kits

Measuring Oxidants and Oxidative Stress in Biological Systems - Biological Magnetic Resonance ◽

10.1007/978-3-030-47318-1_4 ◽

2020 ◽

pp. 39-60 ◽

Cited By ~ 1

Author(s):

Bryan Gardiner ◽

Julie A. Dougherty ◽

Devasena Ponnalagu ◽

Harpreet Singh ◽

Mark Angelos ◽

...

Keyword(s):

Oxidative Stress ◽

Oxidative Stress Markers ◽

Stress Markers

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In vitro effect of vanadyl sulfate on cultured primary astrocytes: cell viability and oxidative stress markers.

Journal of Applied Toxicology ◽

10.1002/jat.3939 ◽

2020 ◽

Vol 40 (6) ◽

pp. 737-747

Author(s):

Agnieszka Ścibior ◽

Konrad A. Szychowski ◽

Iwona Zwolak ◽

Klaudia Dachowska ◽

Jan Gmiński

Keyword(s):

Oxidative Stress ◽

Cell Viability ◽

Vanadyl Sulfate ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Primary Astrocytes ◽

Vitro Effect ◽

And Oxidative Stress

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Has seasonality an effect on oxidative stress markers and trace elements levels in patients who undergo in vitro fertilization treatment?

Clinica Chimica Acta ◽

10.1016/j.cca.2019.03.1314 ◽

2019 ◽

Vol 493 ◽

pp. S628

Author(s):

V. Castañón Bernardo ◽

C. Barneo Caragol ◽

S. Rodríguez González ◽

I. Vega Naredo ◽

L. Sánchez Castro ◽

...

Keyword(s):

Oxidative Stress ◽

Trace Elements ◽

In Vitro Fertilization ◽

Oxidative Stress Markers ◽

Vitro Fertilization ◽

Stress Markers

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Anti-allergic activities of Umbelliferone against histamine- and Picryl chloride-induced ear edema by targeting Nrf2/iNOS signaling in mice

BMC Complementary Medicine and Therapies ◽

10.1186/s12906-021-03384-1 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Younas ◽

Ashrafullah Khan ◽

Omer Shehzad ◽

Eun Kyoung Seo ◽

Alev Onder ◽

...

Keyword(s):

Oxidative Stress ◽

Immunoglobulin E ◽

Statistical Significance ◽

Giemsa Staining ◽

Related Factor ◽

Oxidative Stress Markers ◽

Ear Edema ◽

Stress Markers ◽

Picryl Chloride

Abstract Background The current study was aimed to investigate the anti-allergic activities of the Umbelliferone (UMB) against the acute Histamine and chronic Picryl chloride (PiCl)-induced allergy in mice. UMB is a coumarin derivative (isolated from Angelica decursiva) found in various parts of the plants such as flowers, roots and, stems isolated from the plants of Umbelliferae family. Methods The UMB (1, 10, 50 mg/kg) was administered intraperitoneally (i.p) half an h before or 2 h after the induction of allergic ear edema. The acute ear edema was induced by histamine (intradermally, i.d), while the chronic ear edema was induced by painting the PiCl (sensitized with the toluene) on the ear. The antioxidants and oxidative stress markers were assessed. The histological changes were assessed using Hematoxylin and eosin (H and E) and giemsa staining. The immunohistochemistry studies were performed to assess the expression of the nuclear factor erythroid 2-related factor 2 (Nrf2) and inducible nitric oxide synthase (iNOS). The data was analyzed using one-way ANOVA tests followed by Tukey’s test with p < 0.05 was chosen as criteria for statistical significance. Results UMB treatment markedly reduced the allergic ear edema and ear weight compared to the negative control. Furthermore, the UMB attenuated the oxidative stress markers, while induced the antioxidants enzymes. Similarly, the UMB treatment significantly attenuated the serum immunoglobulin E (IgE) level. The UMB treatment markedly improved the histological parameters using H and E staining and Giemsa staining. The UMB administration induced the Nrf2 expression, while attenuated the iNOS expression. Furthermore, the computational analysis was performed to assess the interaction of the UMB with the various protein targets and to determine the mechanism of interaction with the target proteins. Conclusion In conclusion, the UMB treatment significantly alleviated the allergic symptoms, attenuating the oxidative stress, improved the histological features using in vivo and computational approaches.

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The Molecular Mechanisms of Hypoglycemic Properties and Safety Profiles of Swietenia Macrophylla Seeds Extract: A Review

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.6972 ◽

2021 ◽

Vol 9 (F) ◽

pp. 370-388

Author(s):

Ratih Dewi Yudhani ◽

Dwi Aris Agung Nugrahaningsih ◽

Eti Nurwening Sholikhah ◽

Mustofa Mustofa

Keyword(s):

Oxidative Stress ◽

Phosphoenolpyruvate Carboxykinase ◽

Molecular Mechanisms ◽

Glucose Transporter ◽

Density Lipoprotein ◽

Thiobarbituric Acid ◽

In Vitro Cytotoxicity ◽

Swietenia Macrophylla

BACKGROUND: Insulin resistance (IR) is known as the root cause of type 2 diabetes; hence, it is a substantial therapeutic target. Nowadays, studies have shifted the focus to natural ingredients that have been utilized as a traditional diabetes treatment, including Swietenia macrophylla. Accumulating evidence supports the hypoglycemic activities of S. macrophylla seeds extract, although its molecular mechanisms have yet to be well-established. AIM: This review focuses on the hypoglycemic molecular mechanisms of S. macrophylla seeds extract and its safety profiles. METHODS: An extensive search of the latest literature was conducted from four main databases (PubMed, Scopus, Science Direct, and Google Scholar) using several keywords: “swietenia macrophylla, seeds, and diabetes;” “swietenia macrophylla, seeds, and oxidative stress;” “swietenia macrophylla, seeds, and inflammation;” “swietenia macrophylla, seeds, and GLUT4;” and “swietenia macrophylla, seeds, and toxicities.” RESULTS: The hypoglycemic activities occur through modulating several pathways associated with IR and T2D pathogenesis. The seeds extract of S. macrophylla modulates oxidative stress by decreasing malondialdehyde (MDA), oxidized low-density lipoprotein, and thiobarbituric acid-reactive substances while increasing antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). Another propose mechanism is the modulating of the inflammatory pathway by attenuating nuclear factor kappa β, tumor necrosis factor α, inducible nitric oxide synthase, and cyclooxygenase 2. Some studies have shown that the extract can also control phosphatidylinositol-3-kinase/ Akt (PI3K/Akt) pathway by inducing glucose transporter 4, while suppressing phosphoenolpyruvate carboxykinase. Moreover, in vitro cytotoxicity and in vivo toxicity studies supported the safety profile of S. macrophylla seeds extract with the LD50 higher than 2000 mg/kg. CONCLUSION: The potential of S. macrophylla seeds as antidiabetic candidate is supported by many studies that have documented their non-toxic and hypoglycemic effects, which involve several molecular pathways.

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Ferulic acid attenuates osteoporosis induced by glucocorticoid through regulating the GSK-3β/Lrp-5/ERK signalling pathways

Physiology International ◽

10.1556/2060.2021.00180 ◽

2021 ◽

Author(s):

Wei Zhou ◽

Bo Chen ◽

Jingbo Shang ◽

Renbo Li

Keyword(s):

Oxidative Stress ◽

Ferulic Acid ◽

Saline Control ◽

Control Group ◽

Oxidative Stress Markers ◽

Expression Ratio ◽

Stress Markers ◽

Osteogenic Markers ◽

Gsk 3Β

Abstract Objective To evaluate in-vivo and in-vitro effects of ferulic acid (FA) on glucocorticoid-induced osteoarthritis (GIO) to establish its possible underlying mechanisms. Methods The effects of FA on cell proliferation, cell viability (MTT assay), ALP activity, and mineralization assay, and oxidative stress markers (ROS, SOD, GSH LDH and MDA levels) were investigated by MC3T3-E1 cell line. Wistar rats received standard saline (control group) or dexamethasone (GC, 2 mg−1 kg) or DEX+FA (50 and 100 mg−1 kg) orally for 8 weeks. Bone density, micro-architecture, bio-mechanics, bone turnover markers and histo-morphology were determined. The expression of OPG, RANKL, osteogenic markers, and other signalling proteins was assessed employing quantitative RT-PCR and Western blotting. Results The findings indicated the elevation of ALP mRNA expressions, osteogenic markers (Runx-2, OSX, Col-I, and OSN), and the β-Catenin, Lrp-5 and GSK-3β protein expressions. FA showed the potential to increase MC3T3-E1 cell differentiation, proliferation, and mineralization. FA increased oxidative stress markers (SOD, MDA, and GSH) while decreasing ROS levels and lactate dehydrogenase release in GIO rats. The OPG/RANKL mRNA expression ratio was increased by FA, followed by improved GSK-3β and ERK phosphorylation with enhanced mRNA expressions of Lrp-5 and β-catenin. Conclusion These findings showed that FA improved osteoblasts proliferation with oxidative stress suppression by controlling the Lrp-5/GSK-3β/ERK pathway in GIO, demonstrating the potential pathways involved in the mechanism of actions of FA in GIO therapy.

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