scholarly journals A pilot study on molecular diagnosis of Hapalotrema mistroides (Digenea: Spirorchiidae) infection in blood samples of live loggerhead turtles Caretta caretta

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Erica Marchiori ◽  
Giorgia Dotto ◽  
Cinzia Tessarin ◽  
Mario Santoro ◽  
Andrea Affuso ◽  
...  
Keyword(s):  
Pilot Study ◽  
Molecular Diagnosis ◽  
Caretta Caretta ◽  
Blood Samples ◽  
Loggerhead Turtles

scholarly journals Effects of Delayed Sample Processing on Determination of Total and High Molecular Weight (HMW) Adiponectin in Serum and Plasma: A Pilot Study

2016 ◽  
Vol 8 (3) ◽  
pp. 19
Author(s):  
Choaping Ng ◽  
Felicity J Rose ◽  
Sahar Keshvari ◽  
Marina M Reeves ◽  
Goce Dimeski ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Pilot Study ◽  
High Molecular Weight ◽  
Accurate Determination ◽  
Mean Difference ◽  
Serum Samples ◽  
Blood Samples ◽  
Hmw Adiponectin ◽  
Total Adiponectin

<p>Adiponectin is a beneficial adipocyte-secreted hormone, which circulates in a variety of multimeric forms termed low and high molecular weight (LMW/HMW). Effectiveness of clinical therapeutic trials which target adiponectin rely on accurate determination of circulating total and HMW adiponectin levels but the accuracy may be influenced by variations in sample handling processes. The aim of this pilot study was to investigate the effects of delayed processing of blood samples on the concentration of total and HMW adiponectin.</p><p>Materials and Methods: Fasting blood samples were collected for analysis of total and HMW adiponectin concentrations in EDTA plasma and serum from eight healthy participants.  Samples were centrifuged post 15 min storage at 4<sup>o</sup>C as the comparative ‘ideal’ method or after up to 72 h of refrigerated storage or 6 h at room temperature. Total and HMW adiponectin concentrations were measured by ELISA.</p><p>Results: Under ideal handling conditions measurements of total and HMW adiponectin concentrations were significantly higher in serum than in plasma (mean difference: -1.3 µg/mL [95% CI: -1.6, -1.0], p&lt;0.001; and, -0.6 µg/mL [95% CI: -0.7, -0.5], p&lt;0.001, respectively).  Storage of blood samples at 4<sup>o</sup>C for 72 h resulted in significant reductions in concentration of total adiponectin in serum (mean difference: -1.4 µg/mL [95% CI: -2.0, -0.8], p=0.001) and HMW adiponectin in plasma (mean difference: -0.6 µg/mL [95%CI: -0.9, -0.2], p=0.007), compared with ideal conditions.  Further analysis of serum samples showed a significant decrease in total adiponectin concentration after 6 h storage at 4<sup>o</sup>C (mean difference: -1.4 µg/mL [95% CI: -2.0, -0.8], p=0.001) compared with ideal conditions.</p><p>Conclusions: Delayed processing of samples may have differential effects on the concentration of total and HMW adiponectin in serum or plasma. Larger studies are warranted for clinical intervention trials.</p>

Nest placement by loggerhead turtles, Caretta caretta

1993 ◽  
Vol 45 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Graeme C. Hays ◽  
John R. Speakman
Keyword(s):  
Caretta Caretta ◽  
Nest Placement ◽  
Loggerhead Turtles

scholarly journals Helminth communities of loggerhead turtles (Caretta caretta) from Central and Western Mediterranean Sea: The importance of host's ontogeny

2010 ◽  
Vol 59 (3) ◽  
pp. 367-375 ◽  
Author(s):  
Mario Santoro ◽  
Francisco J. Badillo ◽  
Simonetta Mattiucci ◽  
Giuseppe Nascetti ◽  
Flegra Bentivegna ◽  
...  
Keyword(s):  
Mediterranean Sea ◽  
Caretta Caretta ◽  
Western Mediterranean ◽  
Western Mediterranean Sea ◽  
Helminth Communities ◽  
Loggerhead Turtles

scholarly journals The practical use of computed tomography in evaluation of shell lesions in six loggerhead turtles (Caretta caretta)

2016 ◽  
Vol 61 (No. 7) ◽  
pp. 394-398 ◽  
Author(s):  
F. Spadola ◽  
G. Barillaro ◽  
M. Morici ◽  
A. Nocera ◽  
Z. Knotek
Keyword(s):  
Computed Tomography ◽  
Spinal Cord ◽  
Physical Examination ◽  
Lumbar Vertebra ◽  
Caretta Caretta ◽  
Tube Insertion ◽  
Computed Tomographic ◽  
Non Invasive ◽  
Loggerhead Turtles ◽  
Invasive Method

Six adult loggerhead turtles were found stranded and were rescued near Sicily within a period of 12 months. Macroscopically apparent lesions of the shell were present. After thorough physical examination, ketamine-dexmedetomidine-atipamezole induction and tracheal tube insertion all six patients underwent computed tomographic examination under inhalant anaesthesia with isoflurane. A vertebral lesion at the level of the 3<sup>rd</sup> thoracic-lumbar vertebra with vertebral lamina and the vertebral body being involved without compression of the spinal cord, a vertebral lesion at the level of the 7<sup>th</sup> thoracic-lumbar vertebra and a vertebral lesion at the level of the 8<sup>th</sup> thoracic-lumbar vertebra were recorded in the first female. Loss of the shell near the left carapace-plastron bridge, with massive haemorrhage and compression of organs were present in the second female. The remaining four turtles had only superficial lesions with no involvement of bones and organs of the coelom. Computed tomography was proved to be a valuable non-invasive method for clinical examination of stranded sea turtles.

Origin of juvenile loggerhead turtles (Caretta caretta) in a tropical developmental habitat in Caribbean Panamá

2002 ◽  
Vol 5 (2) ◽  
pp. 125-133 ◽  
Author(s):  
Tag N. Engstrom ◽  
Peter A. Meylan ◽  
Anne B. Meylan
Keyword(s):  
Caretta Caretta ◽  
Loggerhead Turtles
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