scholarly journals Molecular epidemiology of Streptococcus pneumoniae isolated from pediatric community-acquired pneumonia in pre-conjugate vaccine era in Western China

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Zhuoxin Liang ◽  
Jinjian Fu ◽  
Ling Li ◽  
Rongsong Yi ◽  
Shaolin Xu ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Multidrug Resistant ◽  
Community Acquired Pneumonia ◽  
Western China ◽  
Molecular Characteristics ◽  
High Coverage ◽  
Pediatric Infections ◽  
Coverage Rates ◽  
Sequence Types ◽  
High Coverage Rate

Abstract Background Streptococcus pneumoniae (S. pneumoniae) is one of the most common pathogens which can cause morbidity and mortality in pediatric infections worldwide. This study aimed to describe the phenotypic and molecular characteristics of community-acquired pneumonia (CAP)-causing S. pneumoniae recovered from children in Western China. Methods We retrospectively enrolled pediatric patients younger than 5 years diagnosed with CAP. All 419 S. pneumoniae isolates were tested for antibiotic susceptibility, serotypes, virulence genes, resistance genes, and sequence types. The potential relationships between molecular characteristics were tested by correspondence analysis. Results Most of S. pneumoniae isolates were resistant to erythromycin, tetracycline, clindamycin and trimethoprim-sulfamethoxazole, with 93.8% isolates classified as multidrug resistant. The dominant STs were ST271 (30.8%) and ST320 (12.2%), while the prevailing serotypes were 19F (46.8%), 6B (11.5%), 23F (9.5%) and 19A (9.3%). The coverage rates of PCV-7 and PCV-13 were 73.03% and 86.16%, while the coverage rates of PCV13 among children aged < 1 year and 1–2 years were high in 93.18% and 93.62%. We also observed that CC271 expressed more of mef (A/E), lytA, rlrA and sipA than non-CC271 isolates. Moreover, there were strong corresponding relationships between molecular characteristics. Conclusions The high coverage rate of PCV13 suggests the necessity of introducing the PCV13 vaccine in Western China. Our findings underscore the value of monitoring multiple molecular characteristics to provide new guidance for developing future pneumococcal vaccines.

scholarly journals Genotypic and phenotypic characteristics of Streptococcus pneumoniae from community-acquired pneumonia patients and healthy asymptomatic participants in Sichuan province, China

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shihui Peng ◽  
Hongyu Ren ◽  
Jianping Deng ◽  
Na Zhao ◽  
Yinan Li ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Antibiotic Susceptibility ◽  
Multidrug Resistant ◽  
Community Acquired Pneumonia ◽  
Upper Respiratory Tract ◽  
High Coverage ◽  
Asymptomatic Carriers ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Coverage Rates

Abstract Background Streptococcus pneumoniae (S. pneumoniae) is the common cause of community-acquired pneumonia (CAP) and is also found in the upper respiratory tract of healthy people. Hence, the study aimed to compare the serotypes, virulence/pili genes, and antibiotic susceptibility of S. pneumoniae from healthy asymptomatic participants and CAP patients. Methods Streptococcus pneumoniae were retrospectively collected from health asymptomatic participants and CAP patients in Sichuan, China. The serotypes were tested by multiplex polymerase chain reaction (PCR) or Quellung reaction. Antibiotic susceptibility testing was performed using the broth microdilution method. The molecular epidemiology of S. pneumoniae was analyzed by multilocus sequence typing (MLST). Additionally, the presence of virulence/pili genes were detected using PCR. Results A total of 83 pneumococcal isolates were collected in the current study. Of these, 52 and 31 isolates were from healthy asymptomatic participants and CAP patients, respectively. Most of S. pneumoniae were resistant to erythromycin (ERY), clindamycin (CLI), tetracycline (TET) and trimethoprim-sulfamethoxazole (SXT). 90.4% isolates were classified as multidrug resistant (MDR). The predominant serotypes were 3, 19F and 19A in the CAP carriers, whereas 3, 6 and 19F were the main serotypes among the asymptomatic carriers. The overall coverage rates of pneumococcal conjugate vaccine (PCV) 10 and PCV13 serotypes were 34.9% and 66.3%, respectively. The predominant sequence types (STs) were ST271, ST320, and ST3397. There were significant differences in some resistance and virulence characteristics between CAP patients and asymptomatic carriers. Additionally, clonal complex (CC) 271 strains had higher percentage in resistance to cefuroxime (CXM) and cefotaxime (CEF), meropenem (MER) and cefepime (CFP), which mainly carried the rlrA and sipA genes. Conclusions High coverage rate of PCV13 and high prevalence of MDR indicated the necessity to expand immunization with PCV13 and rationally use the antibiotics in Sichuan, China. Importantly, long-term surveillance should be conducted to assess effectiveness brought by vaccines. Our findings may supply new guidance for developing new pneumococcal vaccines.

Incidence of childhood pneumonia and serotype and sequence-type distribution inStreptococcus pneumoniaeisolates in Japan

2011 ◽  
Vol 140 (6) ◽  
pp. 1111-1121 ◽  
Author(s):  
J. TANAKA ◽  
N. ISHIWADA ◽  
A. WADA ◽  
B. CHANG ◽  
H. HISHIKI ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Community Acquired Pneumonia ◽  
Sequence Type ◽  
Annual Incidence ◽  
Blood Isolate ◽  
Blood Samples ◽  
Number Of Children ◽  
Common Serotypes ◽  
Coverage Rates ◽  
Sequence Types

SUMMARYThe 7-valent pneumococcal conjugate vaccine (PCV7) is reported to decrease the incidence of community-acquired pneumonia (CAP) in children. To determine the annual incidence of CAP before the introduction of PCV7, we counted the number of children hospitalized with CAP between 2008 and 2009 in Chiba City, Japan. We investigated serotype and multilocus sequence typing (MLST) forStreptococcus pneumoniaeisolates in CAP cases. The annual incidence of hospitalized CAP in children aged <5 years was 17·6 episodes/1000 child-years. In 626 episodes,S. pneumoniaewas dominant in 14·7% and 0·8% of sputum and blood samples, respectively. The most common serotypes were 6B, 23F and 19F. The coverage rates of PCV7 were 66·7% and 80% in sputum samples and blood samples, respectively. MLST analysis revealed 37 sequence types. Furthermore, 54·1% of the sputum isolates and 40% of the blood isolate were related to international multidrug-resistant clones.

scholarly journals Antibiotic Resistance and Molecular Biological Characteristics of Non-13-Valent-Pneumococcal Conjugate Vaccine Serogroup 15 Streptococcus pneumoniae Isolated From Children in China

2022 ◽  
Vol 12 ◽  
Author(s):  
Wei Shi ◽  
Qianqian Du ◽  
Lin Yuan ◽  
Wei Gao ◽  
Qing Wang ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Streptococcus Pneumoniae ◽  
Conjugate Vaccine ◽  
Pneumococcal Conjugate Vaccine ◽  
Resistance Rate ◽  
Diffusion Method ◽  
Molecular Characteristics ◽  
Quellung Reaction ◽  
Susceptibility Rate ◽  
Sequence Types

Background: The isolation rate of serogroup 15 Streptococcus pneumoniae has been increasing since developing countries began administering the 13-valent pneumococcal conjugate vaccine.Methods: We detected the antibiotic resistance and molecular characteristics of 126 serogroup 15 S. pneumoniae strains isolated from children in China. Serotypes were determined via the Quellung reaction. Antibiotic resistance was tested using the E-test or disc diffusion method. Sequence types were assigned via multilocus sequence typing. Data were analyzed using WHONET 5.6 software.Results: The frequencies of S. pneumoniae serotypes 15A, 15B, 15C, and 15F were 29.37, 40.48, 28.57, and 1.59%, respectively. Continuous-monitoring data from Beijing showed that the annual isolation rates of serogroup 15 S. pneumoniae were 7.64, 7.17, 2.58, 4.35, 3.85, 7.41, and 10.53%, respectively, from 2013 to 2019. All 126 serogroup 15 strains were susceptible to vancomycin and ceftriaxone. The non-susceptibility rate to penicillin was 78.57%. All strains were resistant to erythromycin with high minimum inhibitory concentrations (MICs). The multidrug resistance rate was 78.57%. The most common clonal complexes were CC3397, CC6011, CC10088, CC9785, and ST8589.Conclusion: Serogroup 15 S. pneumoniae is common among children in China, and these strains should be continuously monitored.

scholarly journals The epidemiological characteristics of pediatric Streptococcus pneumoniae isolated from inpatients and outpatients at Beijing Children’s Hospital

2021 ◽  
Author(s):  
shuang Lyu ◽  
Wei Shi ◽  
Fang Dong ◽  
Baoping Xu ◽  
Gang Liu ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Pneumococcal Vaccine ◽  
Epidemiological Data ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Treatment And Prevention ◽  
Coverage Rates ◽  
Epidemiological Characteristics ◽  
Sequence Types

Abstract Background : The epidemiological data of Streptococcus pneumoniae isolates are important for the practice of treatment and prevention. This research aimed to explore the epidemiological characteristics of pediatric S. pneumoniae isolated from outpatients and inpatients. Methods: S. pneumoniae were isolated from unsterile samples of inpatients and outpatients younger than five years old between March 2013 and February 2014. The serotypes were determined by diagnostic pneumococcal antisera, and resistance against 13 antibiotics was tested by either the E-test or the disc diffusion method. The sequence types (STs) were analyzed with multilocus sequence typing (MLST). Results: The five dominant serotypes obtained from inpatients were 19F(32.9%), 19A(20.7%), 23F(10.7%), 6A(10.0%), and 14 (8.6%), while those in the outpatients were 19F (13.6%), 23F (12.9%), 6A (10.0%), 6B (10.0%), and 19A (7.9%). The coverage rates of the 7-, 10- and 13-valent pneumococcal vaccine formulations were high. The non-susceptibility to penicillin, cefuroxime, imipenem, erythromycin, and trimethoprim-sulfamethoxazole among the inpatient isolates were 7.1%, 92.8%, 65.7%, 100%, and 85.0%, respectively, while those among the outpatient isolates were 0.7%, 50.0%, 38.6%, 96.4%, and 65.7%, respectively. There were 45 and 81 STs detected from the pneumococci isolated from inpatients and outpatients, respectively. CC271 was more prevalent in inpatients. Conclusions : The pneumococcal vaccine related serotypes were still prevalent either in inpatient department or in outpatient department, which with serious antibiotic resistance. These results might be helpful for understanding the epidemiology of S. pneumoniae in Beijing. PCVs can prevent vaccine related serotypes. Therefore, universal immunization of PCVs should be implemented to prevent the spread of vaccine related serotypes of S. pneumoniae .

scholarly journals The epidemiological characteristics of pediatric Streptococcus pneumoniae isolated from inpatients and outpatients at Beijing Children's Hospital

2020 ◽  
Author(s):  
Shuang Lyu
Keyword(s):  
Streptococcus Pneumoniae ◽  
Epidemiological Data ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Treatment And Prevention ◽  
Coverage Rates ◽  
Epidemiological Characteristics ◽  
Sequence Types ◽  
Inpatient Department

Abstract Background: The epidemiological data of Streptococcus pneumoniae isolates are important for the practice of treatment and prevention. This research aiming to explore the epidemiological characteristics of pediatric S. pneumoniae isolated from outpatients and inpatients.Methods: S. pneumoniae were isolated from unsterile samples of inpatients and outpatients younger than five years old between March 2013 and February 2014. The serotypes were determined by diagnostic pneumococcal antisera, and resistance against 13 antibiotics was tested by either the E-test or the disc diffusion method. The sequence types (STs) were analyzed with multilocus sequence typing (MLST).Results: The five dominant serotypes obtained from inpatients were 19F(32.9%), 19A(20.7%), 23F(10.7%), 6A(10.0%), and 14 (8.6%), while those in the outpatients were 19F (13.6%), 23F (12.9%), 6A (10.0%), 6B (10.0%), and 19A (7.9%). The coverage rates of the 7-, 10- and 13-valent pneumococcal vaccine formulations were high. The non-susceptibility to penicillin, cefuroxime, imipenem, erythromycin, and trimethoprim-sulfamethoxazole among the inpatient isolates were 7.1%, 92.8%, 65.7%, 100%, and 85.0%, respectively, while those among the outpatient isolates were 0.7%, 50.0%, 38.6%, 96.4%, and 65.7%, respectively. There were 45 and 81 STs detected from the pneumococci from inpatients and outpatients, respectively. CC271 was more prevalent in inpatients.Conclusions: The vaccine related serotypes were still prevalent either in inpatient department or in outpatient department, the prevalent serotypes and MLSTs with serious antibiotic resistance. More effective measures should be implemented to prevent S. pneumoniae diseases.

scholarly journals Pathogenic bacterial profile and drug resistance analysis of community-acquired pneumonia in older outpatients with fever

2018 ◽  
Vol 46 (11) ◽  
pp. 4596-4604 ◽  
Author(s):  
Ying Luan ◽  
Yuling Sun ◽  
Shuhong Duan ◽  
Ping Zhao ◽  
Zhongying Bao
Keyword(s):  
Staphylococcus Aureus ◽  
Drug Resistance ◽  
Streptococcus Pneumoniae ◽  
Older Patients ◽  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Community Acquired Pneumonia ◽  
Gram Negative Bacteria ◽  
E Coli ◽  
Drug Sensitivity Test

Objectives To study the pathogenic bacterial profile and drug resistance in older patients with community-acquired pneumonia (CAP) in outpatients with fever, and provide evidence to diagnose and treat CAP timely and accurately. Methods We studied older (>60 years) patients with CAP in Beijing Shijitan Hospital from 2016 to 2017. Pathogenic bacteria from sputum of patients were isolated and identified and their resistance to antibiotics was tested. Risk factors for multidrug-resistant CAP (MDR-CAP) and clinical outcomes were analyzed. Results A total of 5563 outpatients with fever were recruited and 391 had CAP. A total of 117 isolates of pathogenic bacteria were obtained from 176 CAP cases. The main pathogenic bacteria were Klebsiella pneumoniae (27.4%), Escherichia coli (17.9%), Staphylococcus aureus (12.0%), Pseudomonas aeruginosa (10.3%), and Streptococcus pneumoniae (9.4%). A drug sensitivity test (DST) showed that K. pneumoniae, E. coli, and P. aeruginosa had good sensitivity to imipenem, cefoperazone/sulbactam, piperacillin/tazobactam, and amikacin. Staphylococcus aureus and Streptococcus pneumoniae had strong sensitivity to vancomycin, linezolid, and levofloxacin. Previous multiple antibiotic treatment was an independent risk factor for MDR-CAP. Conclusions Gram-negative bacteria are the main pathogenic bacteria in older patients with CAP. Identification and DSTs of pathogens could enable accurate diagnosis and treatment of CAP.

scholarly journals The Epidemiological Characteristics of Pediatric Streptococcus Pneumoniae Isolated From Inpatients And Outpatients At Beijing Children’s Hospital

2021 ◽  
Author(s):  
Shuang Lyu ◽  
Wei Shi ◽  
Fang Dong ◽  
Baoping Xu ◽  
Gang Liu ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Pneumococcal Vaccine ◽  
Epidemiological Data ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Treatment And Prevention ◽  
Coverage Rates ◽  
Epidemiological Characteristics ◽  
Sequence Types

Abstract Background: The epidemiological data of Streptococcus pneumoniae isolates are important for the practice of treatment and prevention. This research aimed to explore the epidemiological characteristics of pediatric S. pneumoniae isolated from outpatients and inpatients.Methods: S. pneumoniae were isolated from unsterile samples of inpatients and outpatients younger than five years old between March 2013 and February 2014. The serotypes were determined by diagnostic pneumococcal antisera, and resistance against 13 antibiotics was tested by either the E-test or the disc diffusion method. The sequence types (STs) were analyzed with multilocus sequence typing (MLST).Results: The five dominant serotypes obtained from inpatients were 19F(32.9%), 19A(20.7%), 23F(10.7%), 6A(10.0%), and 14 (8.6%), while those in the outpatients were 19F (13.6%), 23F (12.9%), 6A (10.0%), 6B (10.0%), and 19A (7.9%). The coverage rates of the 7-, 10- and 13-valent pneumococcal vaccine formulations were high. The non-susceptibility to penicillin, cefuroxime, imipenem, erythromycin, and trimethoprim-sulfamethoxazole among the inpatient isolates were 7.1%, 92.8%, 65.7%, 100%, and 85.0%, respectively, while those among the outpatient isolates were 0.7%, 50.0%, 38.6%, 96.4%, and 65.7%, respectively. There were 45 and 81 STs detected from the pneumococci isolated from inpatients and outpatients, respectively. CC271 was more prevalent in inpatients.Conclusions: The pneumococcal vaccine related serotypes were still prevalent either in inpatient department or in outpatient department, which with serious antibiotic resistance. These results might be helpful for understanding the epidemiology of S. pneumoniae in Beijing. PCVs can prevent vaccine related serotypes. Therefore, universal immunization of PCVs should be implemented to prevent the spread of vaccine related serotypes of S. pneumoniae.

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