scholarly journals B- and N-doped carbon dots by one-step microwave hydrothermal synthesis: tracking yeast status and imaging mechanism

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Bo Tian ◽  
Tianxin Fu ◽  
Yang Wan ◽  
Yun Ma ◽  
Yanbo Wang ◽  
...  
Keyword(s):  
Yeast Cell ◽  
Carbon Dots ◽  
Biomedical Application ◽  
Basic Research ◽  
Growth Status ◽  
Dose Time ◽  
Microwave Hydrothermal ◽  
One Step ◽  
Doped Carbon Dots ◽  
Energy Dependent

Abstract Background Carbon dots (CDs) are widely used in cell imaging due to their excellent optical properties, biocompatibility and low toxicity. At present, most of the research on CDs focuses on biomedical application, while there are few studies on the application of microbial imaging. Results In this study, B- and N-doped carbon dots (BN-CDs) were prepared from citric acid, ethylenediamine, and boric acid by microwave hydrothermal method. Based on BN-CDs labeling yeast, the dead or living of yeast cell could be quickly identified, and their growth status could also be clearly observed. In order to further observe the morphology of yeast cell under different lethal methods, six methods were used to kill the cells and then used BN-CDs to label the cells for imaging. More remarkably, imaging of yeast cell with ultrasound and antibiotics was significantly different from other imaging due to the overflow of cell contents. In addition, the endocytosis mechanism of BN-CDs was investigated. The cellular uptake of BN-CDs is dose, time and partially energy-dependent along with the involvement of passive diffusion. The main mechanism of endocytosis is caveolae-mediated. Conclusion BN-CDs can be used for long-term stable imaging of yeast, and the study provides basic research for applying CDs to microbiol imaging. Graphical Abstract

Multifunctional Biomedical Applications of Nitrogen and Sulfur Co-Doped Carbon Dots

2021 ◽  
Vol 17 (8) ◽  
pp. 1598-1611
Author(s):  
Dan Zhao ◽  
Rui Zhang ◽  
Mengyu Xu ◽  
Xincai Xiao ◽  
Haiyan Zhao ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Biomedical Application ◽  
Carbon Sources ◽  
Sulfur Source ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
One Step ◽  
Onion Epidermal Cells ◽  
Doped Carbon Dots ◽  
Co Doped

Multifunctional carbon dots have drawn considerable attention due to their potential biomedical application value. We report the preparation of blue-green fluorescence-emitting, multifunctional, nitrogen-and-sulfur co-doped carbon dots (N, S-CDs) synthesized via a one-step process using 1-thioglycerol as a sulfur source, glucose and citric acid as carbon sources, and polyethyleneimine as a nitrogen source. Because of abundant amino and sulfur content, the CDs exhibited high sensibility and selectivity for detecting Cu2+ (detection limit: 0.01 μM, linear range: 0.025 to 50 μM). Fast and sensitive detection of tiopronin was also achieved on the basis of the fluorescence “off-on” mode considering the strong affinity between tiopronin and Cu2+. The N, S-CDs exhibited good biocompatibility as determined by fluorescence imaging using onion epidermal cells and gram-positive bacteria. The CDs also exhibited excellent antimicrobial ability against the gram-positive bacteria. Our results indicate that these novel N, S-CDs could be ideal candidates for several biochemical applications such as antibacterial treatment and detection of small biomolecules.

Fast one-step synthesis of N-doped carbon dots by pyrolyzing ethanolamine

2014 ◽  
Vol 2 (36) ◽  
pp. 7477-7481 ◽  
Author(s):  
Xinwei Dong ◽  
Yanjie Su ◽  
Huijuan Geng ◽  
Zhongli Li ◽  
Chao Yang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Carbon Dots ◽  
High Yield ◽  
One Step ◽  
Doped Carbon Dots

N-doped CDs can be obtained directly with high yield by pyrolyzing ethanolamine in air within just 7 minutes with the assistance of hydrogen peroxide.

One step synthesis of N-doped carbon dots/hydroxyapatite:Eu,Gd composite with dual-emissive and solid-state photoluminescence

2020 ◽  
Vol 508 ◽  
pp. 144862 ◽  
Author(s):  
Qing Chang ◽  
Wei Xu ◽  
Qianqian Chen ◽  
Chaorui Xue ◽  
Ning Li ◽  
...  
Keyword(s):  
Solid State ◽  
Carbon Dots ◽  
One Step ◽  
Doped Carbon Dots

Fe3+ detection, bioimaging, and patterning based on bright blue-fluorescent N-doped carbon dots

The Analyst ◽  
2020 ◽  
Vol 145 (16) ◽  
pp. 5450-5457 ◽  
Author(s):  
Lin Li ◽  
Lihong Shi ◽  
Jing Jia ◽  
Dan Chang ◽  
Chuan Dong ◽  
...  
Keyword(s):  
Hydrothermal Treatment ◽  
Carbon Dots ◽  
Cellular Imaging ◽  
One Step ◽  
Doped Carbon Dots ◽  
Bright Blue

The bright blue fluorescent N-doped carbon dots (N-CDs) was successfully fabricated by one-step hydrothermal treatment of astragalus and was exploited for cellular imaging and patterning.

scholarly journals Aggregation induced red shift emission of phosphorus doped carbon dots

RSC Advances ◽  
2017 ◽  
Vol 7 (51) ◽  
pp. 32225-32228 ◽  
Author(s):  
Ying Li ◽  
Hechun Lin ◽  
Chunhua Luo ◽  
Yunqiu Wang ◽  
Chunli Jiang ◽  
...  
Keyword(s):  
Hydrothermal Method ◽  
Carbon Dots ◽  
Red Shift ◽  
One Step ◽  
Doped Carbon Dots ◽  
Phosphorus Doped ◽  
Method Show

Phosphorous doped carbon dots (P-CDs), prepared by a one-step hydrothermal method, show the phenomenon of aggregation induced red shift emission (AIRSE).

scholarly journals Detection of Melamine Based on the Fluorescence Changes of Nitrogen-Doped Carbon Dots

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Wenzhi Yin ◽  
Chaoqun Ma ◽  
Tuo Zhu ◽  
Jiao Gu ◽  
Chun Zhu ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Detection Method ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Mercury Ions ◽  
Measuring Range ◽  
Nitrogen Doped ◽  
One Step ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

In order to determine the concentration of melamine, nitrogen-doped carbon dots (NCDs) were synthesized in one step as a fluorescent probe. Uric acid and diethylenetriamine were used as carbon source and nitrogen source, respectively. The experimental results showed that the fluorescence of NCDs can be quenched by mercury ions (Hg2+). Due to the strong coordination affinity between the carbon-nitrogen heterocyclic of melamine and Hg2+, part of Hg2+ coordinated with melamine when melamine was mixed with Hg2+. Then, the fluorescence of the added NCDs was quenched by the remaining Hg2+. Therefore, the concentration of melamine could be determined. The results show that the method has high sensitivity in wide measuring range that the linear ranges are 50–400 μg/L and 800–2500 μg/L, and the R2 is 0.997 and 0.988, respectively, with the limit of detection (LOD) of 21.76 μg/L. The NCDs are easy to fabricate, and the detection method is easy to implement. In this study, a new method for melamine detection was established, and the proposed method for melamine detection can provide some insights for food safety detection.

Nitrogen and sulfur co-doped carbon dots synthesis via one step hydrothermal carbonization of green alga and their multifunctional applications

2019 ◽  
Vol 147 ◽  
pp. 1038-1047 ◽  
Author(s):  
Yuehai Li ◽  
Fengjiao Liu ◽  
Jiabai Cai ◽  
Xuguang Huang ◽  
Luxiu Lin ◽  
...  
Keyword(s):  
Green Alga ◽  
Carbon Dots ◽  
Hydrothermal Carbonization ◽  
One Step ◽  
Doped Carbon Dots ◽  
Co Doped
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