scholarly journals Proteomics and ultrastructural analysis of Hermetia illucens (Diptera: Stratiomyidae) larval peritrophic matrix

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yu-Bo Lin ◽  
Jing-Jing Rong ◽  
Xun-Fan Wei ◽  
Zhuo-Xiao Sui ◽  
Jinhua Xiao ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ultrastructural Analysis ◽  
Peritrophic Matrix ◽  
Molecular Architecture ◽  
Hermetia Illucens ◽  
Manual Curation ◽  
Liquid Chromatography Tandem Mass ◽  
Proteomics Research ◽  
Scanning Electron

Abstract Background The black soldier fly (Hermetia illucens) has significant economic potential. The larvae can be used in financially viable waste management systems, as they are voracious feeders able to efficiently convert low-quality waste into valuable biomass. However, most studies on H. illucens in recent decades have focused on optimizing their breeding and bioconversion conditions, while information on their biology is limited. Methods About 200 fifth instar well-fed larvae were sacrificed in this work. The liquid chromatography-tandem mass spectrometry and scanning electron microscopy were employed in this study to perform a proteomic and ultrastructural analysis of the peritrophic matrix (PM) of H. illucens larvae. Results A total of 565 proteins were identified in the PM samples of H. illucen, of which 177 proteins were predicted to contain signal peptides, bioinformatics analysis and manual curation determined 88 proteins may be associated with the PM, with functions in digestion, immunity, PM modulation, and others. The ultrastructure of the H. illucens larval PM observed by scanning electron microscopy shows a unique diamond-shaped chitin grid texture. Conclusions It is the first and most comprehensive proteomics research about the PM of H. illucens larvae to date. All the proteins identified in this work has been discussed in details, except several unnamed or uncharacterized proteins, which should not be ignored and need further study. A comparison of the ultrastructure between H. illucens larval PM and those of other insects as observed by SEM indicates that the PM displays diverse textures on an ultra-micro scale and we suscept a unique diamond-shaped chitin grid texture may help H. illucens larval to hold more food. This work deepens our understanding of the molecular architecture and ultrastructure of the H. illucens larval PM.

scholarly journals Fine Structure of Maxillary Palps in Adults of Hermetia illucens (Diptera: Stratiomyidae)

2020 ◽  
Author(s):  
M Pezzi ◽  
C Scapoli ◽  
M Bharti ◽  
M J Faucheux ◽  
M Chicca ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sexual Dimorphism ◽  
Hermetia Illucens ◽  
The Family ◽  
Sensory Structures ◽  
First Time ◽  
Maxillary Palps ◽  
Scanning Electron

Abstract A relevant species in waste management but also in forensic, medical, and veterinary sciences is the black soldier fly, Hermetia illucens (Linnaeus; Diptera: Stratiomyidae). An ultrastructural study by scanning electron microscopy (SEM) was conducted for the first time on maxillary palps of both sexes, describing in detail the morphology and distribution of sensilla and microtrichia. The maxillary palps, composed of two segments, show sexual dimorphism in length and shape. In both sexes, the first segment is covered only by microtrichia, but the second one is divided into two parts: the proximal one, covered only by microtrichia, and the distal one containing both microtrichia and sensory structures. These structures include two types of sensory pits and one of chaetic sensilla. Due to sexual dimorphism in palp size, females have a higher number of sensory pits. The sexual dimorphism of palps and the presence and role of sensilla in H. illucens was discussed in comparison to other species of the family Stratiomyidae and of other Diptera. This study may represent a base for further investigations on mouthpart structures of this species, involved in key physiological activities, such as feeding, mating and oviposition.

scholarly journals Three Dimensional Ultrastructural Analysis of CNS Axons Using Serial Ion-Abrasion Scanning Electron Microscopy (SIA-SEM)

2010 ◽  
Vol 16 (S2) ◽  
pp. 188-189
Author(s):  
G Kidd ◽  
A Avishai ◽  
N Ohno ◽  
X Yin ◽  
N Avishai ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Three Dimensional ◽  
Ultrastructural Analysis ◽  
Scanning Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.

Ultrastructural Analysis of Electrofused Protoplasts from Pansy and Wild Viola by Scanning Electron Microscopy

1998 ◽  
Vol 1 (4) ◽  
pp. 288-295
Author(s):  
Tadahiko Sato ◽  
Oh Chang Kwon ◽  
Yong Mo Chung ◽  
Chung Han Chung ◽  
Hiroshi Miyake ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ultrastructural Analysis ◽  
Scanning Electron

scholarly journals Experimental white piedra: a robust approach to ultrastructural analysis, scanning electron microscopy and etiological discoveries

2015 ◽  
Vol 25 (1) ◽  
pp. 79-81 ◽  
Author(s):  
Cicero P. Inácio ◽  
Ana Paula S. Rocha ◽  
Renan do N. Barbosa ◽  
Neiva T. Oliveira ◽  
Josineide C. Silva ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ultrastructural Analysis ◽  
Robust Approach ◽  
White Piedra ◽  
Scanning Electron

scholarly journals Potensi Tepung Magot Black Soldier Fly (Hermetia illucens) sebagai Agen Antibakteri dan Immunomodulator Pakan Ternak Unggas secara In vitro

2021 ◽  
Vol 39 (2) ◽  
pp. 112
Author(s):  
Desy Cahya Widianingrum ◽  
Melinda Erdya Krismaputri ◽  
Listya Purnamasari
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Scanning Electron

Tepung Magot dalam pakan unggas tidak hanya dapat digunakan sebagai alternatif sumber protein namun juga diharapkan memiliki efek antibakterial dan immunomodulator. Penelitian ini menggunakan metode in vitro untuk mengetahui efek antimikrobial dan immunomodulator tepung magot. Uji sensitivitas dilakukan dengan metode disc difusi agar, uji aktivitas fagositosis diamati pada makrofag peritoneum mencit Balb-C jantan berumur 8 minggu terhadap Staphylococcus aureus (S. aureus), serta uji tantang S. aureus terhadap tepung magot dilihat di bawah scanning electron microscopy (SEM). Data hasil uji sensitivitas dan pengamatan dengan teknik SEM dilaporkan secara deskriptif. Perbedaan aktivitas fagositosis makrofag antar perlakuan diuji dengan analisis varian satu arah dengan uji lanjut honestly significant difference (HSD) Berdasar hasil penelitian diketahui bahwa tepung memiliki 38,22% kandungan protein dengan profil asam amino yang lengkap. Kandungan asam amino tertinggi pada tepung magot adalah (7685,84 mg/kg), aspartat (5864,19 mg/kg), leusin (5034,31 mg/kg). Asam lemak esensial yang terkandung pada tepung magot adalah asam laurat (13,39%) Hasil uji sensitivitas diketahui tepung magot tidak memberikan zona hambat pada bakteri S. aureus. Introduksi tepung magot pada fagositosis secara in vitro dapat meningkatkan kinerja makrofag dengan perannya seperti opsonin berdasar pengamatan SEM. Kesimpulan dari penelitian ini adalah tepung magot potensial digunakan sebagai imunomodulator natural dan pengganti protein pakan unggas.

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